Biotic and abiotic stress tolerance in plants

ABSTRACT

Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

RELATIONSHIP TO COPENDING APPLICATIONS

This application claims the benefit of Application No. 60/961,403, filed Jul. 20, 2007 (pending). This application is a continuation-in-part of application Ser. No. 10/286,264, filed Nov. 1, 2002 (pending), which is a divisional of application Ser. No. 09/533,030, filed Mar. 22, 2000 (abandoned), which claims the benefit of Application No. 60/125,814, filed Mar. 23, 1999. This application is a continuation-in-part of application Ser. No. 10/675,852, filed Sep. 30, 2003 (pending). This application is a continuation-in-part of application Ser. No. 11/479,226, filed Jun. 30, 2006 (pending), which is a continuation-in-part of application Ser. No. 09/713,994, filed Nov. 16, 2000 (abandoned), which claims the benefit of Application No. 60/166,228, filed Nov. 17, 1999, which also claims the benefit of Application No. 60/197,899, filed Apr. 17, 2000, which also claims the benefit of Application No. 60/227,439, filed Aug. 22, 2000. This application is a continuation-in-part of application Ser. No. 10/669,824, filed Sep. 23, 2003, which is a continuation-in-part of, 09/823,676, filed Mar. 30, 2001 (issued as U.S. Pat. No. 6,717,034). This application is a continuation-in-part of application Ser. No. 11/725,235, filed Mar. 16, 2007 (pending), which is a divisional of application Ser. No. 10/225,068, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,193,129), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, and also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, and also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; application Ser. No. 10/225,068 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). This application is a continuation-in-part of application Ser. No. 11/728,567, filed Mar. 26, 2007, which is a divisional of application Ser. No. 10/225,066, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,238,860), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, and also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, and also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001. application Ser. No. 10/225,066 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). This application is a continuation-in-part of application Ser. No. 11/375,241, filed Mar. 16, 2006 (pending), which claims the benefit of Application No. 60/713,952, filed Aug. 31, 2005. application Ser. No. 11/375,241 is also a continuation-in-part of application Ser. No. 10/225,067, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,135,616), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, which also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, and also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001. application Ser. No. 10/225,067 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). This application is a continuation-in-part of application Ser. No. 11/069,255, filed Feb. 28, 2005 (pending), which is a continuation-in-part of application Ser. No. 10/112,887, filed Mar. 18, 2002 (abandoned). This application is a continuation-in-part of application Ser. No. 10/374,780, filed Feb. 25, 2003 (pending), which is a continuation-in-part of application Ser. No. 09/934,455, filed Aug. 22, 2001 (abandoned), which is a continuation-in-part of application Ser. No. 09/713,994, 11/16/2000 (abandoned), which is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), which also claims priority to Application No. 60/227,439, filed Aug. 22, 2000. application Ser. No. 10/374,780 is also a continuation-in-part of application Ser. No. 10/225,068, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,193,129), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, and also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, and also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001. application Ser. No. 10/225,068 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). application Ser. No. 10/374,780 is also a continuation-in-part of application Ser. No. 10/225,066, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,238,860), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, which also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, which also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; application Ser. No. 10/225,066 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). application Ser. No. 10/374,780 is also a continuation-in-part of application Ser. No. 10/225,067, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,135,616), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, and also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, and also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; application Ser. No. 10/225,067 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). This application is a continuation-in-part of application Ser. No. 10/546,266, filed Aug. 19, 2005 (pending), which is a '371 National Stage filing of International Application No. PCT/US2004005654, filed Feb. 25, 2004 (converted), which is a continuation-in-part of application Ser. No. 10/374,780, filed Feb. 25, 2003 (pending), and is also a continuation-in-part of application Ser. No. 10/675,852, filed Sep. 30, 2003 (pending). This application is also a continuation-in-part of application Ser. No. 10/412,699, filed Apr. 10, 2003 (pending), which is a continuation-in-part of application Ser. No. 10/295,403, filed Nov. 15, 2002 (abandoned), which is a divisional of application Ser. No. 09/394,519, filed Sep. 13, 1999 (abandoned), which claims the benefit of Application No. 60/101,349, filed Sep. 22, 1998, which also claims the benefit of Application No. 60/103,312, filed Oct. 6, 1998, which also claims the benefit of Application No. 60/108,734, filed Nov. 17, 1998, which also claims the benefit of Application No. 60/113,409, filed Dec. 22, 1998. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 09/489,376, filed Jan. 21, 2000 (abandoned), which claimed priority to Application No. 60/116,841, filed Jan. 22, 1999. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/302,267, filed Nov. 22, 2002 (issued as U.S. Pat. No. 7,223,904), which is a divisional of application Ser. No. 09/506,720, filed Feb. 17, 2000 (abandoned), which claims the benefit of Application No. 60/120,880, filed Feb. 18, 1999, which also claims the benefit of Application No. 60/121,037, filed Feb. 22, 1999, which also claims the benefit of Application No. 60/124,278, filed Mar. 11, 1999, which also claims the benefit of Application No. 60/129,450, filed Apr. 15, 1999, which also claims the benefit of Application No. 60/135,134, filed May 20, 1999, which also claims the benefit of Application No. 60/144,153, filed Jul. 15, 1999, which also claims the benefit of Application No. 60/161,143, filed Oct. 22, 1999, which also claims the benefit of Application No. 60/162,656, filed Nov. 1, 1999. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/278,173, filed Oct. 21, 2002 (abandoned), which is a divisional of application Ser. No. 09/533,392, filed Mar. 22, 2000 (abandoned), which claims the benefit of Application No. 60/125,814, filed Mar. 23, 1999. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 09/533,029, filed Mar. 22, 2000 (issued as U.S. Pat. No. 6,664,446), which claims the benefit of Application No. 60/125,814, filed Mar. 23, 1999. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/278,536, filed Oct. 22, 2002 (abandoned), which is a divisional of application Ser. No. 09/532,591, filed Mar. 22, 2000 (abandoned), which claims priority to Application No. 60/125,814, filed Mar. 23, 1999. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 09/713,994, filed Nov. 16, 2000 (abandoned), which claims the benefit of Application No. 60/166,228, filed Nov. 17, 1999, which also claims the benefit of Application No. 60/197,899, filed Apr. 17, 2000, which also claims the benefit of Application No. 60/227,439, filed Aug. 22, 2000. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 09/819,142, filed Mar. 27, 2001. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 09/934,455, filed Aug. 22, 2001 (abandoned), which is a continuation-in-part of application Ser. No. 09/713,994, filed Nov. 16, 2000 (abandoned), which is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), which also claim the benefit of Application No. 60/227,439, filed Aug. 22, 2000. application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/225,068, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,193,129), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, which also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, which also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; and, application Ser. No. 10/225,068 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/225,066, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,238,860), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, which also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, which also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; and, application Ser. No. 10/225,066 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/225,067, filed Aug. 9, 2002 (issued as U.S. Pat. No. 7,135,616), which claims the benefit of Application No. 60/310,847, filed Aug. 9, 2001, which also claims the benefit of Application No. 60/336,049, filed Nov. 19, 2001, which also claims the benefit of Application No. 60/338,692, filed Dec. 11, 2001; and, application Ser. No. 10/225,067 is also a continuation-in-part of application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned), and is also a continuation-in-part of application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned). application Ser. No. 10/412,699 is also a continuation-in-part of application Ser. No. 10/374,780, filed Feb. 25, 2003 (pending). This application is a continuation-in-part of application Ser. No. 10/559,441, filed Dec. 2, 2005 (pending), which is a '371 National Stage filing of International Application No. PCT/US2004/017768, filed Jun. 4, 2004 (converted), which is a continuation-in-part of application Ser. No. 10/456,882, filed Jun. 6, 2003 (abandoned). This application is a continuation-in-part of application Ser. No. 11/642,814, filed Dec. 20, 2006 (pending), which is a divisional of application Ser. No. 10/666,642, filed Sep. 18, 2003 (issued as U.S. Pat. No. 7,196,245), which claims the benefit of Application No. 60/411,837, filed Sep. 18, 2002, and also claims the benefit of Application No. 60/434,166, filed Dec. 17, 2002, and also claims the benefit of Application No. 60/465,809, filed Apr. 24, 2003. This application is a continuation-in-part of application Ser. No. 10/714,887, filed Nov. 13, 2003 (pending), which is a continuation-in-part of application Ser. No. 10/456,882, filed Jun. 6, 2003 (abandoned); and application Ser. No. 10/714,887 is also a continuation-in-part of application Ser. No. 10/666,642, filed Sep. 18, 2003 (issued as U.S. Pat. No. 7,196,245), which claims the benefit of Application No. 60/411,837, filed Sep. 18, 2002, which also claims the benefit of Application No. 60/434,166, filed Dec. 17, 2002, which also claims the benefit of Application No. 60/465,809, filed Apr. 24, 2003. This application is a continuation-in-part of application Ser. No. 11/435,388, filed May 15, 2006 (pending), which is a continuation-in-part of International Application No. PCT/US04/37584, filed Nov. 12, 2004 (converted), which is a continuation-in-part of application Ser. No. 10/714,887, filed Nov. 13, 2003 (pending), and also claims the benefit of Application No. 60/527,658, filed Dec. 5, 2003, and also claims the benefit of Application No. 60/542,928, filed Feb. 5, 2004. This application is a continuation-in-part of application Ser. No. 11/632,390, filed Jan. 11, 2007 (pending), which is a '371 National Stage filing of International Application No. PCT/US2005/025010, filed Jul. 14, 2005 (converted), which claims the benefit of Application No. 60/588,405, filed Jul. 14, 2004. This application is a continuation-in-part of application Ser. No. 12/064,961, filed Feb. 26, 2008 (pending), which is a continuation-in-part of PCT application PCT/US06/34615, filed Aug. 31, 2006 (expired), which claims the benefit of Application No. 60/713,952, filed Aug. 31, 2006. This application is a continuation-in-part of International Application no. PCT/US2006/34615, filed Aug. 31, 2006 (pending), which claims the benefit of Application No. 60/713,952, filed Aug. 31, 2005. This application is a continuation-in-part of application Ser. No. 10/903,236, filed Jul. 30, 2004 (pending), which is a continuation-in-part of application Ser. No. 10/456,882, filed Jun. 6, 2003 (abandoned), and is also a continuation-in-part of application Ser. No. 10/666,642, filed Sep. 18, 2003 (issued as U.S. Pat. No. 7,196,245), which claims the benefit of Application No. 60/411,837, filed Sep. 18, 2002, and also claims the benefit of Application No. 60/465,809, filed Apr. 24, 2003. This application is a continuation-in-part of application Ser. No. 11/699,973, filed Jan. 29, 2007 (pending), which is a continuation-in-part of International Application No. PCT/US2005-027151, filed Jul. 29, 2005 (converted), which is a continuation-in-part of application Ser. No. 10/903,236, filed Jul. 30, 2004 (pending). This application is a continuation-in-part of application Ser. No. 10/870,198, filed Jun. 16, 2004 (pending), which claims the benefit of Application No. 60/565,948, filed Apr. 26, 2004, which also claims the benefit of Application No. 60/527,658, filed Dec. 5, 2003, which also claims the benefit of Application No. 60/542,928, filed Feb. 5, 2005; and, application Ser. No. 10/870,198 is also a continuation-in-part of application Ser. No. 10/669,824, filed Sep. 23, 2003 (pending), which is a continuation-in-part of application Ser. No. 09/823,676, filed Mar. 30, 2001 (issued as U.S. Pat. No. 6,717,034). This application is a continuation-in-part of application Ser. No. 10/838,616, filed May 4, 2004 (pending), which claims the benefit of Application No. 60/565,948, filed Apr. 26, 2004, and is a continuation-in-part of application Ser. No. 10/685,922, filed Oct. 14, 2003 (abandoned). This application is a continuation-in-part of International Application No. PCT/US2007/17321, filed Aug. 7, 2006 (pending), which claims the benefit of Application No. 60/836,243, filed Aug. 7, 2006. This application is a continuation-in-part of application Ser. No. 11/705,903, filed Feb. 12, 2007 (pending), which is a continuation-in-part of International Application No. PCT/US2006/34615, filed Aug. 31, 2006 (converted), which claims the benefit of Application No. 60/713,952, filed Aug. 31, 2005. This application is a continuation-in-part of application Ser. No. 11/821,448, filed Jun. 22, 2007 (pending), which claims priority to Application No. 60/817,886, filed Jun. 29, 2006. This application is a continuation-in-part of International Application No. PCT/US2007/09124, filed Apr. 12, 2007 (pending), which claims priority to Application No. 60/791,663, filed Apr. 12, 2006. This application is a continuation-in-part of application Ser. No. 11/986,992, filed Nov. 26, 2007 (pending), which is a division of application Ser. No. 10/412,699, filed Apr. 10, 2003 (pending). The contents of all applications herein are incorporated by referenced in their entirety.

ACKNOWLEDGEMENT

This invention was supported in part by NSF SBIR grants DMI-0450162, DMI-0349577, and DMI-0320074. The U.S. government may have certain rights in this invention.

JOINT RESEARCH AGREEMENT

The claimed invention, in the field of functional genomics and the characterization of plant genes for the improvement of plants, was made by or on behalf of Mendel Biotechnology, Inc. and Monsanto Company as a result of activities undertaken within the scope of a joint research agreement, and in effect on or before the date the claimed invention was made.

FIELD OF THE INVENTION

The present invention relates to plant genomics and plant improvement.

BACKGROUND OF THE INVENTION

Abiotic stress and impact on yield. Water deficit is a common component of many plant stresses. Water deficit occurs in plant cells when the whole plant transpiration rate exceeds the water uptake. In addition to drought, other stresses, such as salinity and low temperature, produce cellular dehydration (McCue and Hanson, 1990).

Salt (and drought) stress signal transduction consists of ionic and osmotic homeostasis signaling pathways. The ionic aspect of salt stress is signaled via the SOS pathway where a calcium-responsive SOS3-SOS2 protein kinase complex controls the expression and activity of ion transporters such as SOS1. The pathway regulating ion homeostasis in response to salt stress has been reviewed recently by Xiong and Zhu (2002a).

The osmotic component of salt-stress involves complex plant reactions that are possibly overlapping with drought- and/or cold-stress responses. Common aspects of drought-, cold- and salt-stress response have been reviewed by Xiong and Zhu (2002). These include:

Abscisic acid (ABA) biosynthesis is regulated by osmotic stress at multiple steps. Both ABA-dependent and -independent osmotic stress signaling first modify constitutively expressed transcription factors, leading to the expression of early response transcriptional activators, which then activate downstream stress tolerance effector genes.

Based on the commonality of many aspects of cold, drought, and salt stress responses, it can be concluded that genes that increase tolerance to cold or salt stress can also improve drought stress protection. In fact, this has already been demonstrated for transcription factors (in the case of AtCBF/DREB 1) and for other genes such as OsCDPK7 (Saijo et al. (2000)), or AVP1 (a vacuolar pyrophosphatase-proton-pump, Gaxiola et al. (2001)).

Heat stress often accompanies conditions of low water availability. Heat itself is seen as an interacting stress and adds to the detrimental effects caused by water deficit conditions. Evaporative demand exhibits near exponential increases with increases in daytime temperatures and can result in high transpiration rates and low plant water potentials (Hall et al. (2000)). High-temperature damage to pollen almost always occurs in conjunction with drought stress, and rarely occurs under well-watered conditions. Thus, separating the effects of heat and drought stress on pollination is difficult. Combined stress can alter plant metabolism in novel ways; therefore, understanding the interaction between different stresses may be important for the development of strategies to enhance stress tolerance by genetic manipulation.

Plant pathogens and impact on yield. While a number of plant pathogens exist that may significantly impact yield or affect the quality of plant products, specific attention is being given in this application to a small subset of these microorganisms. These include:

Sclerotinia. Sclerotinia sclerotiorum is a necrotrophic ascomycete that causes destructive rots of numerous plants (Agrios (1997)). Sclerotinia stem rot is a significant pathogen of soybeans in the northern U.S., and Canada.

Botrytis. Botrytis causes blight or gray mold, a disease of plants that infects a wide array of herbaceous annual and perennial plants. Environmental conditions favorable to this pathogen can significantly impact ornamental plants, vegetables and fruit. Botrytis infections generally occur in spring and summer months following cool, wet weather, and may be particularly damaging when these conditions persist for several days.

Fusarium. Fusarium or vascular wilt may affect a variety of plant host species. Seedlings of developing plants may be infected with Fusarium, resulting in the grave condition known as “damping-off”. Fusarium species also cause root, stem, and corn rots of growing plants and pink or yellow molds of fruits during post-harvest storage. The latter affect ornamentals and vegetables, particularly root crops, tubers, and bulbs.

Drought-Disease Interactions. Plant responses to biotic and abiotic stresses are governed by complex signal transduction networks. There appears to be significant interaction between these networks, both positive and negative. An understanding of the complexity of these interactions will be necessary to avoid unintended consequences when altering plant signal transduction pathways to engineer drought or disease resistance.

Transcription factors (TFs) and other genes involved in both abiotic and biotic stress resistance. Despite the evidence for negative cross-talk between drought and disease response pathways, a number of genes have been shown to function in both pathways, indicating possible convergence of the signal transduction pathways. There are numerous examples of genes that are inducible by multiple stresses. For instance, a global TxP (transcriptional profile) analysis revealed classes of transcription factor that are mainly induced by abiotic stresses or disease, but also a class of transcription factors induced both by abiotic stress and bacterial infection (Chen et al. (2002a)).

Implications for crop improvement. Plant responses to drought and disease interact at a number of levels. Although dry conditions do not favor most pathogens, plant defenses may be weakened by metabolic stress or hormonal cross-talk, increasing vulnerability to pathogens that can infect under drought conditions. However, there is also evidence for convergence of abiotic and biotic stress response pathways, based on genes that confer tolerance to multiple stresses. Given our incomplete understanding of these signaling interactions, plants with positive alterations in one stress response should be examined carefully for possible alterations in other stress responses.

SUMMARY OF THE INVENTION

The present invention pertains to expression vectors, transgenic plants comprising the expression vectors of the invention, and methods for making and using the transgenic plants of the invention. The expression vectors and transgenic plants each comprise a recombinant polynucleotide of the invention that encodes a transcription factor polypeptide. The polypeptide is encompassed by the present invention in that it shares an amino acid or nucleotide percentage identity with any of SEQ ID NO: 1 to 5086 or SEQ ID NO: 5102-5107, or a polypeptide sequence of any of SEQ ID NO: SEQ ID NO 2n−1, where n=1 to 1186, or SEQ ID NO: 2373-3791, or SEQ ID NO: 5107-5111, or SEQ ID NO: 5113-5114, or SEQ ID NO: 5116-5117, or SEQ ID NO: 5119-5120, or SEQ ID NO: 5122-5123, or SEQ ID NO: 5125-5143, or SEQ ID NO: 5145-5149, or SEQ ID NO: 5151-5153, or SEQ ID NO: 5155-5157, or SEQ ID NO: 5159-5160, or SEQ ID NO: 5162-5163, and said percentage identity may be at least about 54%, at least about 55%, at least about 56%, at least about 57%, at least about 58%, at least about 59%, at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or about 100%; or

the recombinant nucleic acid sequence the encodes the polypeptide specifically hybridizes to the complement of a DNA sequence set forth in the Sequence Listing, such as SEQ ID NO 2n−1, where n=1 to 1186, or SEQ ID NO: 3792-5086 or 5102-5106, under stringent conditions comprising two wash steps at least as stringent as 6×SSC at 65° C. of 10-30 minutes for each wash step; or 0.2× to 2×SSC and 0.1% SDS at 50° C. to 65° C. for 10-30 minutes per wash step.

When the polypeptide is overexpressed in a plant, the polypeptide is capable of regulating transcription in the plant and confers to the plant at least one regulatory activity. This results in the plant having an altered trait, as compared to a control plant (e.g., a wild-type plant of the same species, or a non-transformed plant, or a plant transformed with an “empty vector” that does not comprise a recombinant nucleic acid sequence encoding a polypeptide of the invention). The altered trait that is conferred to the plant as a result of expressing the polypeptide may be one (or more) of the following, or any trait listed in Table 36: greater resistance to Erysiphe; greater resistance to Sclerotinia; greater resistance to Botrytis; greater resistance to Fusarium; greater susceptibility to Sclerotinia; greater susceptibility to Botrytis; greater tolerance to Pseudomonas; greater tolerance to dehydration; greater tolerance to drought; greater tolerance to salt; greater tolerance to water deficit conditions; greater tolerance to hyperosmotic stress; greater tolerance to low nitrogen conditions; greater tolerance to low phosphate conditions; greater tolerance to low potassium conditions; greater tolerance to cold; greater tolerance to heat; greater tolerance to sucrose; greater tolerance to mannitol; greater tolerance to glucose; greater tolerance to polyethylene glycol; greater tolerance to glyphosate; greater tolerance to oxidative stress; greater tolerance to freezing; better recovery from drought; more sensitive to cold; more sensitive to low nitrogen conditions; more sensitive to low phosphate conditions; more sensitive to sucrose; more sensitive to mannitol; more sensitive to glucose; more sensitive to drought; more sensitive to heat; more sensitive to hyperosmotic stress; more sensitive to oxidative stress; more sensitive to ethylene; ethylene insensitive when germinated in the dark on 1-aminocyclopropane 1-carboxylic acid; hypersensitive to 1-aminocyclopropane 1-carboxylic acid; decreased sensitivity to ABA; altered C/N sensing; higher starch level; higher proline level; decreased proline level; darker green color; lighter green color; gray color; greater photosynthetic capacity; reduced photosynthesis; increased chlorophyll level; more chlorophyll a and b; higher total nitrogen concentration level; decreased chlorophyll level; more pigment; greater anthocyanin level; greater leaf anthocyanin level; more anthocyanin in leaf petioles; decreased anthocyanin level; greater carotenoid level; greater ABA level; greater seed oil content; greater seed protein content; greater seed oil content; greater seed protein content; greater total seed oil and protein content; increased seed alpha-tocopherol level; higher seed lutein content; decreased seed lutein content; increased seed xanthophyll 1 level; increase in seed 16:1 fatty acids level; increased seed 18:1 fatty acids level; increased seed 18:2 fatty acids and decrease in seed 18:3 fatty acids level; increased seed 18:1 and 18:2 fatty acids level; increased seed 16:0, 18:0, 20:0, and 18:3 fatty acids, decreased seed 18:2, 20:1, 22:1 fatty acids level; decreased seed 20:1 and 22:1 fatty acids level; decrease in seed 18:1 seed fatty acids level; decrease in 18:2 fatty acids level; altered seed glucosinolate profile; up-regulation of genes involved in secondary metabolism; altered leaf prenyl lipids; reduced chlorophyll a and b levels; increased leaf insoluble sugars level; decreased leaf insoluble sugars level; increased galactose level in leaf cell wall; increased leaf xanthophyll; increased leaf rhamnose level; increased leaf mannose; increased leaf fucose level; increased leaf glucosinolate M39480 level; increased leaf glucosinolate M39481 level; decreased leaf rhamnose level; decreased leaf lutein level; more leaf fatty acids; altered leaf fatty acid composition; reduced leaf 16:3 fatty acids; increased in percentage of 16:0 leaf fatty acids; leaf 16:0 level decreased and leaf 16:3 level increased; greater seedling vigor; faster seedling growth; slower growth; late flowering; late developing; early flowering; early developing; glossy leaves; waxy leaves; more lignin; reduced lignin; reduced internode elongation; short internodes; long internodes; defect in cell elongation; greater internode distance; altered cotyledon shape; elongated cotyledons; cotyledon fusion; thicker stem; altered distribution of stem vascular bundles; reduced branching; curled leaves; serrated leaves; curled leaves; ovoid leaves; flat leaves; heart-shaped leaves; longer leaves; narrower leaves; wrinkled leaves; lobed leaves; light green leaves; larger, flatter leaves at late stage of development; greater number of leaves; altered flowers; abnormal flowers; sporadic defects in flower development; reduced fertility; flowers that do not open; floral organs with bract-like features; bolts that terminate without an inflorescence; aerial rosettes; reduced floral organ abscission; delayed floral organ abscission; reductions in flower organ size; larger floral organs; long flower organs; long sepal and petal; poor another dehiscence; little pollen production; no pollen production; poor filament elongation; homeotic transformations; bushy inflorescences; altered inflorescences; flowers bunched together; short inflorescence stems; stunted inflorescence growth; numerous secondary inflorescence meristems; altered inflorescence determinacy; homeotic transformation; terminal flower formation; increased carpel size; wider carpels; ectopic carpel tissue; filamentous carpelloid growths on flower pedicels; loss of flower determinacy; floral organ abscission delayed; altered seed color; pale seeds; smaller seeds; rounded seeds; wrinkled seeds; wrinkled sickle-shaped siliques; reduced flower petal number; reduced flower sepal number; reduced flower stamen number; smaller petals and sepals; delayed senescence; premature senescence; premature leaf senescence; premature flower senescence; trilocular silique; more root mass; reduced secondary root growth; greater leaf and hypocotyl necrosis; short pedicels; short inflorescence stems; altered leaf cell expansion; reduced cell differentiation in meristem; increased necrosis; lethal when constitutively overexpressed; embryo lethal; altered light response; long cotyledons; open cotyledons; oval cotyledons; long hypocotyls; long petioles; leaves in a more upright orientation; constitutive photomorphogensis; more root growth in the dark; greater biomass; larger plants; large darker green rosettes at late stage of development; larger seeds; larger leaves; smaller plants; more root hairs; fewer trichomes; greater trichome size and density; greater trichome density; ectopic trichome formation; ectopic formation of trichomes on abaxial leaf surfaces; greater trichome density on sepals and ectopic trichomes on carpels.

For the methods encompassed by the present invention, an expression vector of the invention may be introduced into a target plant, thus transforming the target plant and producing a transgenic plant having the altered trait as compared to the control plant.

BRIEF DESCRIPTION OF THE SEQUENCE LISTING AND DRAWINGS

The Sequence Listing provides exemplary polynucleotide and polypeptide sequences of the invention. The traits associated with the use of the sequences are included in the Examples.

CD-ROMs Copy 1—Sequence Listing Part, Copy 2—Sequence Listing Part, and Copy 3 (the CRF copy of the Sequence Listing), are read-only memory computer-readable compact discs. Each contains a copy of the Sequence Listing in ASCII text format. The Sequence Listing is named “MBI-0065CIP_ST25.txt”, was created on 14 Mar. 2008, and is 24,895 kilobytes in size. The copies of the Sequence Listing on the CD-ROM discs are hereby incorporated by reference in their entirety.

Where applicable, the figures associated with this application include SEQ ID NOs: in parentheses.

FIG. 1 shows a conservative estimate of phylogenetic relationships among the orders of flowering plants (modified from Soltis et al. (1997)). Those plants with a single cotyledon (monocots) are a monophyletic lade nested within at least two major lineages of dicots; the eudicots are further divided into rosids and asterids. Arabidopsis is a rosid eudicot classified within the order Brassicales; rice is a member of the monocot order Poales. FIG. 1 was adapted from Daly et al. (2001).

For the phylogenetic trees presented in the present Figures, the trees were generally based on a ClustalW alignment of full-length proteins using Mega 2 software (protein sequences are provided in the Sequence Listing). The parameters used include a Gap Opening Penalty:10.00; a Gap Extension Penalty:0.20; Delay divergent sequences:30%; DNA Transitions Weight:0.50; Protein weight matrix:Gonnet series; DNA weight matrix IUB; Use negative matrix:OFF. A FastA formatted alignment was then used to generate each phylogenetic tree in MEGA2 using the neighbor joining algorithm and a p-distance model. A test of phylogeny was done via bootstrap with 1000 replications and Random Seed set to default. Cut off values of the bootstrap tree were set to 50%.

For alignments presented in the Figures, SEQ ID NOs are shown in parentheses.

FIG. 2 shows a phylogenetic tree of CCAAT family proteins. There are three main sub-classes within the family: the HAP2 (also known as the NF-YA subclass), HAP3 (NF-YB subclass) and HAP5 (NF-YC subclass) related proteins. Three additional proteins were identified that did not clearly cluster with any of the three main groups and we have designated these as “HAP-like” proteins.

FIGS. 3A-3B are an alignment of various G481 clade member conserved B domains.

FIG. 4 is a phylogenetic tree of G682 clade member sequences. A node representing a common ancestral sequence to the G682 clade (arrow) defines sequences with potentially related functions to G682.

FIG. 5 shows an alignment of various G682 clade member conserved MYB-like domains.

FIG. 6 depicts a phylogenetic tree of G867 clade member sequences.

FIGS. 7A-7B present an alignment of various G867 clade member conserved AP2 domains.

FIGS. 8A-8B present an alignment of a major portion of various G867 clade member conserved B3 domains.

FIG. 9 is a phylogenetic tree of G1073 clade member sequences and include numerous sequences within the clade that have similar functions of conferring, for example, greater biomass and hyperosmotic stress tolerance. The clade is represented by the bracket.

FIGS. 10A-10C show an alignment of a major portion of various G1073 clade member second conserved domains.

FIG. 11 illustrates a phylogenetic tree of G28 clade member AP2 sequences.

FIGS. 12A-12B show an alignment of various G28 clade member conserved AP2 domains.

FIG. 13 presents a portion of a phylogenetic tree showing the ancestral relationships of the G47 clade and other related AP2 sequences.

FIG. 14 provides an alignment of various G47 clade member conserved AP2 domains.

FIG. 15 shows a phylogenetic tree of G1274 clade member sequences. Clade member WRKY sequences are found within the large box.

FIGS. 16A-16B show an alignment of various G1274 clade member conserved WRKY domains.

FIG. 17 illustrates phylogenetic relationships in the G1792 clade. Clade member AP2 sequences are found within the large box.

FIGS. 18A-18B show an alignment of various G1792 clade member conserved AP2 domains.

FIG. 19 shows an alignment of various G1792 clade member conserved EDLL domains, said domains being characteristic of these related sequences.

FIG. 20 shows a phylogenetic tree of G2999 clade member ZF-HD (zinc finger-homeodomain) sequences.

FIGS. 21A-21B show an alignment of various G2999 clade member conserved ZF domains.

FIG. 22A-22B show an alignment of various G2999 clade member conserved HD domains.

FIG. 23 is a phylogenetic tree of G3086 clade member HLH/MYC sequences.

FIGS. 24A-24B present an alignment of various G3086 clade member conserved bHLH domains.

FIG. 25 is a phylogenetic tree of G1988 clade member Z-CO-like (CONSTANS-like) sequences.

FIG. 26 provides an alignment of various G1988 clade member conserved B-box domains.

FIGS. 27A-27B provide an alignment of various G207 clade member conserved MYB domains.

FIGS. 28A-28C provide an alignment of various G922 clade member conserved SCR domains. FIG. 28A is an alignment of the first SCR domains in each of these proteins, FIG. 28B is an alignment of the second SCR domains in each of these proteins, and FIG. 28C provides an alignment of the third SCR domains in each of these proteins.

FIG. 29 is a phylogenetic tree of G1760 clade member MADS-box sequences.

FIGS. 30A-30B present an alignment of various G1760 clade member conserved MADS domains.

FIG. 31 shows an alignment of various G2053 clade member conserved NAC domains.

FIG. 32 is a phylogenetic tree of 913 clade member AP2 sequences.

FIG. 33 shows an alignment of various G913 clade member conserved AP2 domains.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to polynucleotides and polypeptides for modifying phenotypes of plants, particularly those associated with greater biomass, increased disease resistance, and/or abiotic stress tolerance. Throughout this disclosure, various information sources are referred to and/or are specifically incorporated. The information sources include scientific journal articles, patent documents, textbooks, and World Wide Web browser-inactive page addresses. While the reference to these information sources clearly indicates that they can be used by one of skill in the art, each and every one of the information sources cited herein are specifically incorporated in their entirety, whether or not a specific mention of “incorporation by reference” is noted. The contents and teachings of each and every one of the information sources can be relied on and used to make and use embodiments of the invention.

As used herein and in the appended claims, the singular forms “a”, “an”, and “the” include the plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to “a host cell” includes a plurality of such host cells, and a reference to “a stress” is a reference to one or more stresses and equivalents thereof known to those skilled in the art, and so forth.

DEFINITIONS

“Nucleic acid molecule” refers to an oligonucleotide, polynucleotide or any fragment thereof. It may be DNA or RNA of genomic or synthetic origin, double-stranded or single-stranded, and combined with carbohydrate, lipids, protein, or other materials to perform a particular activity such as transformation or form a useful composition such as a peptide nucleic acid (PNA).

“Polynucleotide” is a nucleic acid molecule comprising a plurality of polymerized nucleotides, e.g., at least about 15 consecutive polymerized nucleotides. A polynucleotide may be a nucleic acid, oligonucleotide, nucleotide, or any fragment thereof. In many instances, a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof. Additionally, the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5′ or 3′ untranslated regions, a reporter gene, a selectable marker, or the like. The polynucleotide can be single-stranded or double-stranded DNA or RNA. The polynucleotide optionally comprises modified bases or a modified backbone. The polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can be combined with carbohydrate, lipids, protein, or other materials to perform a particular activity such as transformation or form a useful composition such as a peptide nucleic acid (PNA). The polynucleotide can comprise a sequence in either sense or antisense orientations. “Oligonucleotide” is substantially equivalent to the terms amplimer, primer, oligomer, element, target, and probe and is preferably single-stranded.

“Gene” or “gene sequence” refers to the partial or complete coding sequence of a gene, its complement, and its 5′ or 3′ untranslated regions. A gene is also a functional unit of inheritance, and in physical terms is a particular segment or sequence of nucleotides along a molecule of DNA (or RNA, in the case of RNA viruses) involved in producing a polypeptide chain. The latter may be subjected to subsequent processing such as chemical modification or folding to obtain a functional protein or polypeptide. A gene may be isolated, partially isolated, or found with an organism's genome. By way of example, a transcription factor gene encodes a transcription factor polypeptide, which may be functional or require processing to function as an initiator of transcription.

Operationally, genes may be defined by the cis-trans test, a genetic test that determines whether two mutations occur in the same gene and that may be used to determine the limits of the genetically active unit (Rieger et al. (1976)). A gene generally includes regions preceding (“leaders”; upstream) and following (“trailers”; downstream) the coding region. A gene may also include intervening, non-coding sequences, referred to as “introns”, located between individual coding segments, referred to as “exons”. Most genes have an associated promoter region, a regulatory sequence 5′ of the transcription initiation codon (there are some genes that do not have an identifiable promoter). The function of a gene may also be regulated by enhancers, operators, and other regulatory elements.

A “recombinant polynucleotide” is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity. For example, the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.

An “isolated polynucleotide” is a polynucleotide, whether naturally occurring or recombinant, that is present outside the cell in which it is typically found in nature, whether purified or not. Optionally, an isolated polynucleotide is subject to one or more enrichment or purification procedures, e.g., cell lysis, extraction, centrifugation, precipitation, or the like.

A “polypeptide” is an amino acid sequence comprising a plurality of consecutive polymerized amino acid residues e.g., at least about 15 consecutive polymerized amino acid residues. In many instances, a polypeptide comprises a polymerized amino acid residue sequence that is a transcription factor or a domain or portion or fragment thereof. Additionally, the polypeptide may comprise: (i) a localization domain; (ii) an activation domain; (iii) a repression domain; (iv) an oligomerization domain; (v) a DNA-binding domain; or the like. The polypeptide optionally comprises modified amino acid residues, naturally occurring amino acid residues not encoded by a codon, non-naturally occurring amino acid residues.

“Protein” refers to an amino acid sequence, oligopeptide, peptide, polypeptide or portions thereof whether naturally occurring or synthetic.

“Portion”, as used herein, refers to any part of a protein used for any purpose, but especially for the screening of a library of molecules which specifically bind to that portion or for the production of antibodies.

A “recombinant polypeptide” is a polypeptide produced by translation of a recombinant polynucleotide. A “synthetic polypeptide” is a polypeptide created by consecutive polymerization of isolated amino acid residues using methods well known in the art. An “isolated polypeptide,” whether a naturally occurring or a recombinant polypeptide, is more enriched in (or out of) a cell than the polypeptide in its natural state in a wild-type cell, e.g., more than about 5% enriched, more than about 10% enriched, or more than about 20%, or more than about 50%, or more, enriched, i.e., alternatively denoted: 105%, 110%, 120%, 150% or more, enriched relative to wild type standardized at 100%. Such an enrichment is not the result of a natural response of a wild-type plant. Alternatively, or additionally, the isolated polypeptide is separated from other cellular components with which it is typically associated, e.g., by any of the various protein purification methods herein.

“Homology” refers to sequence similarity between a reference sequence and at least a fragment of a newly sequenced clone insert or its encoded amino acid sequence.

“Identity” or “similarity” refers to sequence similarity between two polynucleotide sequences or between two polypeptide sequences, with identity being a more strict comparison. The phrases “percent identity” and “% identity” refer to the percentage of sequence similarity found in a comparison of two or more polynucleotide sequences or two or more polypeptide sequences. Closely-related polynucleotides of the invention encoded presently disclosed transcription factors that will have at least about 38% sequence identity including conservative substitutions, or at least about 55% sequence identity, or at least about 56%, or at least about 57%, or at least about 58%, or at least about 59%, or at least about 60%, or at least about 61%, or at least about 62% sequence identity, or at least about 63%, or at least about 64%, or at least about 65%, or at least about 66%, or at least about 67%, or at least about 68%, or at least about 69%, or at least about 70%, or at least about 71%, or at least about 72%, or at least about 73%, or at least about 74%, or at least about 75%, or at least about 76%, or at least about 77%, or at least about 78%, or at least about 79%, or at least about 80%, or at least about 81%, or at least about 82%, or at least about 83%, or at least about 84%, or at least about 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 89%, or at least about 90%, or at least about 91%, or at least about 92%, or at least about 93%, or at least about 94%, or at least about 95%, or at least about 96%, or at least about 97%, or at least about 98%, or at least about 99%, or 100% amino acid residue sequence identity, to a polypeptide of the invention listed in the Sequence Listing or in the present Tables 3-33.

“Sequence similarity” refers to the percent similarity in base pair sequence (as determined by any suitable method) between two or more polynucleotide sequences. Two or more sequences can be anywhere from 0-100% similar, or any integer value therebetween. Identity or similarity can be determined by comparing a position in each sequence that may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same nucleotide base or amino acid, then the molecules are identical at that position. A degree of similarity or identity between polynucleotide sequences is a function of the number of identical, matching or corresponding nucleotides at positions shared by the polynucleotide sequences. A degree of identity of polypeptide sequences is a function of the number of identical amino acids at corresponding positions shared by the polypeptide sequences. A degree of homology or similarity of polypeptide sequences is a function of the number of amino acids at corresponding positions shared by the polypeptide sequences.

“Alignment” refers to a number of nucleotide bases or amino acid residue sequences aligned by lengthwise comparison so that components in common (i.e., nucleotide bases or amino acid residues at corresponding positions) may be visually and readily identified. The fraction or percentage of components in common is related to the homology or identity between the sequences. Alignments such as those of FIGS. 18A-18B may be used to identify conserved domains and relatedness within these domains. An alignment may suitably be determined by means of computer programs known in the art, such as MACVECTOR software (1999) (Accelrys, Inc., San Diego, Calif.).

Two or more sequences may be “optimally aligned” with a similarity scoring method using a defined amino acid substitution matrix such as the BLOSUM62 scoring matrix. The preferred method uses a gap existence penalty and gap extension penalty that arrives at the highest possible score for a given pair of sequences. See, for example, Dayhoff et al. (1978) and Henikoff and Henikoff(1992). The BLOSUM62 matrix is often used as a default scoring substitution matrix in sequence alignment protocols such as Gapped BLAST 2.0. The gap existence penalty is imposed for the introduction of a single amino acid gap in one of the aligned sequences, and the gap extension penalty is imposed for each additional empty amino acid position inserted into an already opened gap. The alignment is defined by the amino acids positions of each sequence at which the alignment begins and ends, and optionally by the insertion of a gap or multiple gaps in one or both sequences, so as to arrive at the highest possible score. Optimal alignment may be accomplished manually or with a computer-based alignment algorithm, such as gapped BLAST 2.0 (Altschul et al, (1997); or at www.ncbi.nlm.nih.gov. See U.S. Patent Application US20070004912.

A “conserved domain” or “conserved region” as used herein refers to a region in heterologous polynucleotide or polypeptide sequences where there is a relatively high degree of sequence identity between the distinct sequences. For example, an “AT-hook” domain”, such as is found in a polypeptide member of AT-hook transcription factor family, is an example of a conserved domain. An “AP2” domain”, such as is found in a polypeptide member of AP2 transcription factor family, is another example of a conserved domain. With respect to polynucleotides encoding presently disclosed transcription factors, a conserved domain is preferably at least nine base pairs (bp) in length. A conserved domain with respect to presently disclosed polypeptides refers to a domain within a transcription factor family that exhibits a higher degree of sequence homology, such as at least about 38% amino acid sequence identity including conservative substitutions, or at least about 42% sequence identity, or at least about 45% sequence identity, or at least about 48% sequence identity, or at least about 50% sequence identity, or at least about 51% sequence identity, or at least about 52% sequence identity, or at least about 53% sequence identity, or at least about 54% sequence identity, or at least about 55% sequence identity, or at least about 56% sequence identity, or at least about 57% sequence identity, or at least about 58% sequence identity, or at least about 59% sequence identity, or at least about 60% sequence identity, or at least about 61% sequence identity, or at least about 62% sequence identity, or at least about 63% sequence identity, or at least about 64% sequence identity, or at least about 65% sequence identity, or at least about 66% sequence identity, or at least about 67% sequence identity, or at least about 68% sequence identity, or at least about 69% sequence identity, or at least about 70% sequence identity, or at least about 71% sequence identity, or at least about 72% sequence identity, or at least about 73% sequence identity, or at least about 74% sequence identity, or at least about 75% sequence identity, or at least about 76% sequence identity, or at least about 77% sequence identity, or at least about 78% sequence identity, or at least about 79% sequence identity, or at least about 80% sequence identity, or at least about 81% sequence identity, or at least about 82% sequence identity, or at least about 83% sequence identity, or at least about 84% sequence identity, or at least about 85% sequence identity, or at least about 86% sequence identity, or at least about 87% sequence identity, or at least about 88% sequence identity, or at least about 89% sequence identity, or at least about 90% sequence identity, or at least about 91% sequence identity, or at least about 92% sequence identity, or at least about 93% sequence identity, or at least about 94% sequence identity, or at least about 95% sequence identity, or at least about 96% sequence identity, or at least about 97% sequence identity, or at least about 98% sequence identity, or at least about 99% sequence identity, or 100% amino acid residue sequence identity, to a conserved domain of a polypeptide of the invention, such as those listed in the present tables or Sequence Listing (e.g., SEQ ID NOs: 2373-3791 or SEQ ID NO: 5107-5111, or SEQ ID NO: 5114, or SEQ ID NO: 5117, or SEQ ID NO: 5120, or SEQ ID NO: 5123, or SEQ ID NO: 5126-5143, or SEQ ID NO: 5146-5149, or SEQ ID NO: 5152-5153, or SEQ ID NO: 5156-5157, or SEQ ID NO: 5160, or SEQ ID NO: 5163). Sequences that possess or encode for conserved domains that meet these criteria of percentage identity, and that have comparable biological activity to the present transcription factor sequences, thus being members of a clade of transcription factor polypeptides, are encompassed by the invention. A fragment or domain can be referred to as outside a conserved domain, outside a consensus sequence, or outside a consensus DNA-binding site that is known to exist or that exists for a particular transcription factor class, family, or sub-family. In this case, the fragment or domain will not include the exact amino acids of a consensus sequence or consensus DNA-binding site of a transcription factor class, family or sub-family, or the exact amino acids of a particular transcription factor consensus sequence or consensus DNA-binding site. Furthermore, a particular fragment, region, or domain of a polypeptide, or a polynucleotide encoding a polypeptide, can be “outside a conserved domain” if all the amino acids of the fragment, region, or domain fall outside of a defined conserved domain(s) for a polypeptide or protein. Sequences having lesser degrees of identity but comparable biological activity are considered to be equivalents.

As one of ordinary skill in the art recognizes, conserved domains may be identified as regions or domains of identity to a specific consensus sequence (see, for example, Riechmann et al. (2000a, 2000b)). Thus, by using alignment methods well known in the art, the conserved domains of the plant transcription factors, for example, for the AT-hook proteins (Reeves and Beckerbauer (2001); and Reeves (2001)), may be determined.

The conserved domains for many of the transcription factor sequences of the invention are listed in Tables 3-33. Also, the polypeptides of Tables 3-33 have conserved domains specifically indicated by amino acid coordinate start and stop sites. A comparison of the regions of these polypeptides allows one of skill in the art (see, for example, Reeves and Nissen (1995)) to identify domains or conserved domains for any of the polypeptides listed or referred to in this disclosure.

“Complementary” refers to the natural hydrogen bonding by base pairing between purines and pyrimidines. For example, the sequence A-C-G-T (5′->3′) forms hydrogen bonds with its complements A-C-G-T (5′->3′) or A-C-G-U (5′->3′). Two single-stranded molecules may be considered partially complementary, if only some of the nucleotides bond, or “completely complementary” if all of the nucleotides bond. The degree of complementarity between nucleic acid strands affects the efficiency and strength of hybridization and amplification reactions. “Fully complementary” refers to the case where bonding occurs between every base pair and its complement in a pair of sequences, and the two sequences have the same number of nucleotides.

The terms “highly stringent” or “highly stringent condition” refer to conditions that permit hybridization of DNA strands whose sequences are highly complementary, wherein these same conditions exclude hybridization of significantly mismatched DNAs. Polynucleotide sequences capable of hybridizing under stringent conditions with the polynucleotides of the present invention may be, for example, variants of the disclosed polynucleotide sequences, including allelic or splice variants, or sequences that encode orthologs or paralogs of presently disclosed polypeptides. Nucleic acid hybridization methods are disclosed in detail by Kashima et al. (1985), Sambrook et al. (1989), and by Haymes et al. (1985), which references are incorporated herein by reference.

In general, stringency is determined by the temperature, ionic strength, and concentration of denaturing agents (e.g., formamide) used in a hybridization and washing procedure (for a more detailed description of establishing and determining stringency, see the section “Identifying Polynucleotides or Nucleic Acids by Hybridization”, below). The degree to which two nucleic acids hybridize under various conditions of stringency is correlated with the extent of their similarity. Thus, similar nucleic acid sequences from a variety of sources, such as within a plant's genome (as in the case of paralogs) or from another plant (as in the case of orthologs) that may perform similar functions can be isolated on the basis of their ability to hybridize with known transcription factor sequences. Numerous variations are possible in the conditions and means by which nucleic acid hybridization can be performed to isolate transcription factor sequences having similarity to transcription factor sequences known in the art and are not limited to those explicitly disclosed herein. Such an approach may be used to isolate polynucleotide sequences having various degrees of similarity with disclosed transcription factor sequences, such as, for example, encoded transcription factors having 38% or greater identity with the conserved domain of disclosed transcription factors.

The terms “paralog” and “ortholog” are defined below in the section entitled “Orthologs and Paralogs”. In brief, orthologs and paralogs are evolutionarily related genes that have similar sequences and functions. Orthologs are structurally related genes in different species that are derived by a specification event. Paralogs are structurally related genes within a single species that are derived by a duplication event.

The term “equivalog” describes members of a set of homologous proteins that are conserved with respect to function since their last common ancestor. Related proteins are grouped into equivalog families, and otherwise into protein families with other hierarchically defined homology types. This definition is provided at the Institute for Genomic Research (TIGR) World Wide Web (www) website, “tigr.org” under the heading “Terms associated with TIGRFAMs”.

In general, the term “variant” refers to molecules with some differences, generated synthetically or naturally, in their base or amino acid sequences as compared to a reference (native) polynucleotide or polypeptide, respectively. These differences include substitutions, insertions, deletions or any desired combinations of such changes in a native polynucleotide of amino acid sequence.

With regard to polynucleotide variants, differences between presently disclosed polynucleotides and polynucleotide variants are limited so that the nucleotide sequences of the former and the latter are closely similar overall and, in many regions, identical. Due to the degeneracy of the genetic code, differences between the former and latter nucleotide sequences may be silent (i.e., the amino acids encoded by the polynucleotide are the same, and the variant polynucleotide sequence encodes the same amino acid sequence as the presently disclosed polynucleotide. Variant nucleotide sequences may encode different amino acid sequences, in which case such nucleotide differences will result in amino acid substitutions, additions, deletions, insertions, truncations or fusions with respect to the similar disclosed polynucleotide sequences. These variations may result in polynucleotide variants encoding polypeptides that share at least one functional characteristic. The degeneracy of the genetic code also dictates that many different variant polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing.

Also within the scope of the invention is a variant of a transcription factor nucleic acid listed in the Sequence Listing, that is, one having a sequence that differs from the one of the polynucleotide sequences in the Sequence Listing, or a complementary sequence, that encodes a functionally equivalent polypeptide (i.e., a polypeptide having some degree of equivalent or similar biological activity) but differs in sequence from the sequence in the Sequence Listing, due to degeneracy in the genetic code. Included within this definition are polymorphisms that may or may not be readily detectable using a particular oligonucleotide probe of the polynucleotide encoding polypeptide, and improper or unexpected hybridization to allelic variants, with a locus other than the normal chromosomal locus for the polynucleotide sequence encoding polypeptide.

“Allelic variant” or “polynucleotide allelic variant” refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations may be “silent” or may encode polypeptides having altered amino acid sequence. “Allelic variant” and “polypeptide allelic variant” may also be used with respect to polypeptides, and in this case the terms refer to a polypeptide encoded by an allelic variant of a gene.

“Splice variant” or “polynucleotide splice variant” as used herein refers to alternative forms of RNA transcribed from a gene. Splice variation naturally occurs as a result of alternative sites being spliced within a single transcribed RNA molecule or between separately transcribed RNA molecules, and may result in several different forms of mRNA transcribed from the same gene. Thus, splice variants may encode polypeptides having different amino acid sequences, which may or may not have similar functions in the organism. “Splice variant” or “polypeptide splice variant” may also refer to a polypeptide encoded by a splice variant of a transcribed mRNA.

As used herein, “polynucleotide variants” may also refer to polynucleotide sequences that encode paralogs and orthologs of the presently disclosed polypeptide sequences. “Polypeptide variants” may refer to polypeptide sequences that are paralogs and orthologs of the presently disclosed polypeptide sequences.

Differences between presently disclosed polypeptides and polypeptide variants are limited so that the sequences of the former and the latter are closely similar overall and, in many regions, identical. Presently disclosed polypeptide sequences and similar polypeptide variants may differ in amino acid sequence by one or more substitutions, additions, deletions, fusions and truncations, which may be present in any combination. These differences may produce silent changes and result in a functionally equivalent transcription factor. Thus, it will be readily appreciated by those of skill in the art, that any of a variety of polynucleotide sequences is capable of encoding the transcription factors and transcription factor homolog polypeptides of the invention. A polypeptide sequence variant may have “conservative” changes, wherein a substituted amino acid has similar structural or chemical properties. Deliberate amino acid substitutions may thus be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues, as long as a significant amount of the functional or biological activity of the transcription factor is retained. For example, negatively charged amino acids may include aspartic acid and glutamic acid, positively charged amino acids may include lysine and arginine, and amino acids with uncharged polar head groups having similar hydrophilicity values may include leucine, isoleucine, and valine; glycine and alanine; asparagine and glutamine; serine and threonine; and phenylalanine and tyrosine. More rarely, a variant may have “non-conservative” changes, e.g., replacement of a glycine with a tryptophan. Similar minor variations may also include amino acid deletions or insertions, or both. Related polypeptides may comprise, for example, additions and/or deletions of one or more N-linked or O-linked glycosylation sites, or an addition and/or a deletion of one or more cysteine residues. Guidance in determining which and how many amino acid residues may be substituted, inserted or deleted without abolishing functional or biological activity may be found using computer programs well known in the art, for example, DNASTAR software (see U.S. Pat. No. 5,840,544).

“Fragment”, with respect to a polynucleotide, refers to a clone or any part of a polynucleotide molecule that retains a usable, functional characteristic. Useful fragments include oligonucleotides and polynucleotides that may be used in hybridization or amplification technologies or in the regulation of replication, transcription or translation. A “polynucleotide fragment” refers to any subsequence of a polynucleotide, typically, of at least about 9 consecutive nucleotides, preferably at least about 30 nucleotides, more preferably at least about 50 nucleotides, of any of the sequences provided herein. Exemplary polynucleotide fragments are the first sixty consecutive nucleotides of the transcription factor polynucleotides listed in the Sequence Listing. Exemplary fragments also include fragments that comprise a region that encodes an conserved domain of a transcription factor. Exemplary fragments also include fragments that comprise a conserved domain of a transcription factor. Exemplary fragments include fragments that comprise an conserved domain of a transcription factor, for example, amino acid residues 30-126 of G3866 (SEQ ID NO: 3677).

Fragments may also include subsequences of polypeptides and protein molecules, or a subsequence of the polypeptide. Fragments may have uses in that they may have antigenic potential. In some cases, the fragment or domain is a subsequence of the polypeptide which performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA-binding site or domain that binds to a DNA promoter region, an activation domain, or a domain for protein-protein interactions, and may initiate transcription. Fragments can vary in size from as few as 3 amino acid residues to the full length of the intact polypeptide, but are preferably at least about 30 amino acid residues in length and more preferably at least about 60 amino acid residues in length.

The invention also encompasses production of DNA sequences that encode transcription factors and transcription factor derivatives, or fragments thereof, entirely by synthetic chemistry. After production, the synthetic sequence may be inserted into any of the many available expression vectors and cell systems using reagents well known in the art. Moreover, synthetic chemistry may be used to introduce mutations into a sequence encoding transcription factors or any fragment thereof.

“Derivative” refers to the chemical modification of a nucleic acid molecule or amino acid sequence. Chemical modifications can include replacement of hydrogen by an alkyl, acyl, or amino group or glycosylation, pegylation, or any similar process that retains or enhances biological activity or lifespan of the molecule or sequence.

The term “plant” includes whole plants, shoot vegetative organs/structures (for example, leaves, stems and tubers), roots, flowers and floral organs/structures (for example, bracts, sepals, petals, stamens, carpels, anthers and ovules), seed (including embryo, endosperm, and seed coat) and fruit (the mature ovary), plant tissue (for example, vascular tissue, ground tissue, and the like) and cells (for example, guard cells, egg cells, and the like), and progeny of same. The class of plants that can be used in the method of the invention is generally as broad as the class of higher and lower plants amenable to transformation techniques, including angiosperms (monocotyledonous and dicotyledonous plants), gymnosperms, ferns, horsetails, psilophytes, lycophytes, bryophytes, and multicellular algae.

A “control plant” as used in the present invention refers to a plant cell, seed, plant component, plant tissue, plant organ or whole plant used to compare against transgenic or genetically modified plant for the purpose of identifying an enhanced phenotype in the transgenic or genetically modified plant. A control plant may in some cases be a transgenic plant line that comprises an empty vector or marker gene, but does not contain the recombinant polynucleotide of the present invention that is expressed in the transgenic or genetically modified plant being evaluated. In general, a control plant is a plant of the same line or variety as the transgenic or genetically modified plant being tested. A suitable control plant would include a genetically unaltered or non-transgenic plant of the parental line used to generate a transgenic plant herein.

A “transgenic plant” refers to a plant that contains genetic material not found in a wild-type plant of the same species, variety or cultivar. The genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty. Typically, the foreign genetic material has been introduced into the plant by human manipulation, but any method can be used as one of skill in the art recognizes.

A transgenic plant may contain an expression vector or cassette. The expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the controlled expression of polypeptide. The expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant. A plant refers to a whole plant as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.

“Wild type” or “wild-type”, as used herein, refers to a plant cell, seed, plant component, plant tissue, plant organ or whole plant that has not been genetically modified or treated in an experimental sense. Wild-type cells, seed, components, tissue, organs or whole plants may be used as controls to compare levels of expression and the extent and nature of trait modification with cells, tissue or plants of the same species in which a transcription factor expression is altered, e.g., in that it has been knocked out, overexpressed, or ectopically expressed.

A “trait” refers to a physiological, morphological, biochemical, or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye, such as seed or plant size, or can be measured by biochemical techniques, such as detecting the protein, starch, or oil content of seed or leaves, or by observation of a metabolic or physiological process, e.g. by measuring tolerance to water deprivation or particular salt or sugar concentrations, or by the observation of the expression level of a gene or genes, e.g., by employing Northern analysis, RT-PCR, microarray gene expression assays, or reporter gene expression systems, or by agricultural observations such as hyperosmotic stress tolerance or yield. Any technique can be used to measure the amount of, comparative level of, or difference in any selected chemical compound or macromolecule in the transgenic plants, however.

“Trait modification” refers to a detectable difference in a characteristic in a plant ectopically expressing a polynucleotide or polypeptide of the present invention relative to a plant not doing so, such as a wild-type plant. In some cases, the trait modification can be evaluated quantitatively. For example, the trait modification can entail at least about a 2% increase or decrease, or an even greater difference, in an observed trait as compared with a control or wild-type plant. It is known that there can be a natural variation in the modified trait. Therefore, the trait modification observed entails a change of the normal distribution and magnitude of the trait in the plants as compared to control or wild-type plants.

When two or more plants have “similar morphologies”, “substantially similar morphologies”, “a morphology that is substantially similar”, or are “morphologically similar”, the plants have comparable forms or appearances, including analogous features such as overall dimensions, height, width, mass, root mass, shape, glossiness, color, stem diameter, leaf size, leaf dimension, leaf density, internode distance, branching, root branching, number and form of inflorescences, and other macroscopic characteristics, and the individual plants are not readily distinguishable based on morphological characteristics alone.

“Modulates” refers to a change in activity (biological, chemical, or immunological) or lifespan resulting from specific binding between a molecule and either a nucleic acid molecule or a protein.

The term “transcript profile” refers to the expression levels of a set of genes in a cell in a particular state, particularly by comparison with the expression levels of that same set of genes in a cell of the same type in a reference state. For example, the transcript profile of a particular transcription factor in a suspension cell is the expression levels of a set of genes in a cell knocking out or overexpressing that transcription factor compared with the expression levels of that same set of genes in a suspension cell that has normal levels of that transcription factor. The transcript profile can be presented as a list of those genes whose expression level is significantly different between the two treatments, and the difference ratios. Differences and similarities between expression levels may also be evaluated and calculated using statistical and clustering methods.

With regard to transcription factor gene knockouts as used herein, the term “knockout” refers to a plant or plant cell having a disruption in at least one transcription factor gene in the plant or cell, where the disruption results in a reduced expression or activity of the transcription factor encoded by that gene compared to a control cell. The knockout can be the result of, for example, genomic disruptions, including transposons, tilling, and homologous recombination, antisense constructs, sense constructs, RNA silencing constructs, or RNA interference. A T-DNA insertion within a transcription factor gene is an example of a genotypic alteration that may abolish expression of that transcription factor gene.

“Ectopic expression or altered expression” in reference to a polynucleotide indicates that the pattern of expression in, e.g., a transgenic plant or plant tissue, is different from the expression pattern in a wild-type plant or a reference plant of the same species. The pattern of expression may also be compared with a reference expression pattern in a wild-type plant of the same species. For example, the polynucleotide or polypeptide is expressed in a cell or tissue type other than a cell or tissue type in which the sequence is expressed in the wild-type plant, or by expression at a time other than at the time the sequence is expressed in the wild-type plant, or by a response to different inducible agents, such as hormones or environmental signals, or at different expression levels (either higher or lower) compared with those found in a wild-type plant. The term also refers to altered expression patterns that are produced by lowering the levels of expression to below the detection level or completely abolishing expression. The resulting expression pattern can be transient or stable, constitutive or inducible. In reference to a polypeptide, the term “ectopic expression or altered expression” further may relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.

The term “overexpression” as used herein refers to a greater expression level of a gene in a plant, plant cell or plant tissue, compared to expression of that gene in a wild-type plant, cell or tissue, at any developmental or temporal stage. Overexpression can occur when, for example, the genes encoding one or more transcription factors are under the control of a regulatory control element such as a strong or constitutive promoter (e.g., the cauliflower mosaic virus 35S transcription initiation region). Overexpression may also be achieved by placing a gene of interest under the control of an inducible or tissue specific promoter, or may be achieved through integration of transposons or engineered T-DNA molecules into regulatory regions of a target gene. Thus, overexpression may occur throughout a plant, in specific tissues of the plant, or in the presence or absence of particular environmental signals, depending on the promoter or overexpression approach used.

Overexpression may take place in plant cells normally lacking expression of polypeptides functionally equivalent or identical to the present transcription factors. Overexpression may also occur in plant cells where endogenous expression of the present transcription factors or functionally equivalent molecules normally occurs, but such normal expression is at a lower level. Overexpression thus results in a greater than normal production, or “overproduction” of the transcription factor in the plant, cell or tissue.

The term “transcription regulating region” refers to a DNA regulatory sequence that regulates expression of one or more genes in a plant when a transcription factor having one or more specific binding domains binds to the DNA regulatory sequence. Transcription factors of the present invention possess an conserved domain. The transcription factors of the invention also comprise an amino acid subsequence that forms a transcription activation domain that regulates expression of one or more abiotic stress tolerance genes in a plant when the transcription factor binds to the regulating region.

Transcription Factors Modify Expression of Endogenous Genes

A transcription factor may include, but is not limited to, any polypeptide that can activate or repress transcription of a single gene or a number of genes. As one of ordinary skill in the art recognizes, transcription factors can be identified by the presence of a region or domain of structural similarity or identity to a specific consensus sequence or the presence of a specific consensus DNA-binding site or DNA-binding site motif (see, for example, Riechmann et al. (2000a)). The plant transcription factors of the present invention belong to particular transcription factor families indicated in the Tables found herein (see, for example, Riechmann (2000a, 2000b), Reeves and Beckerbauer (2001); and Reeves (2001)).

Generally, the transcription factors encoded by the present sequences are involved in cell differentiation and proliferation and the regulation of growth. Accordingly, one skilled in the art would recognize that by expressing the present sequences in a plant, one may change the expression of autologous genes or induce the expression of introduced genes. By affecting the expression of similar autologous sequences in a plant that have the biological activity of the present sequences, or by introducing the present sequences into a plant, one may alter a plant's phenotype to one with improved traits related to osmotic stresses. The sequences of the invention may also be used to transform a plant and introduce desirable traits not found in the wild-type cultivar or strain. Plants may then be selected for those that produce the most desirable degree of over- or under-expression of target genes of interest and coincident trait improvement.

The sequences of the present invention may be from any species, particularly plant species, in a naturally occurring form or from any source whether natural, synthetic, semi-synthetic or recombinant. The sequences of the invention may also include fragments of the present amino acid sequences. Where “amino acid sequence” is recited to refer to an amino acid sequence of a naturally occurring protein molecule, “amino acid sequence” and like terms are not meant to limit the amino acid sequence to the complete native amino acid sequence associated with the recited protein molecule.

In addition to methods for modifying a plant phenotype by employing one or more polynucleotides and polypeptides of the invention described herein, the polynucleotides and polypeptides of the invention have a variety of additional uses. These uses include their use in the recombinant production (i.e., expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids); as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like; as substrates for cloning e.g., including digestion or ligation reactions; and for identifying exogenous or endogenous modulators of the transcription factors. The polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can comprise a sequence in either sense or antisense orientations.

Expression of genes that encode transcription factors that modify expression of endogenous genes, polynucleotides, and proteins are well known in the art. In addition, transgenic plants comprising isolated polynucleotides encoding transcription factors may also modify expression of endogenous genes, polynucleotides, and proteins. Examples include Peng et al. (1997) and Peng et al. (1999). In addition, many others have demonstrated that an Arabidopsis transcription factor expressed in an exogenous plant species elicits the same or very similar phenotypic response. See, for example, Fu et al. (2001); Nandi et al. (2000); Coupland (1995); and Weigel and Nilsson (1995)).

In another example, Mandel et al. (1992), and Suzuki et al. (2001), teach that a transcription factor expressed in another plant species elicits the same or very similar phenotypic response of the endogenous sequence, as often predicted in earlier studies of Arabidopsis transcription factors in Arabidopsis (see Mandel et al. (1992); Suzuki et al. (2001)). Other examples include Müller et al. (2001); Kim et al. (2001); Kyozuka and Shimamoto (2002); Boss and Thomas (2002); He et al. (2000); and Robson et al. (2001).

In yet another example, Gilmour et al. (1998) teach an Arabidopsis AP2 transcription factor, CBF1, which, when overexpressed in transgenic plants, increases plant freezing tolerance. Jaglo et al. (2001) further identified sequences in Brassica napus which encode CBF-like genes and that transcripts for these genes accumulated rapidly in response to low temperature. Transcripts encoding CBF-like proteins were also found to accumulate rapidly in response to low temperature in wheat, as well as in tomato. An alignment of the CBF proteins from Arabidopsis, B. napus, wheat, rye, and tomato revealed the presence of conserved consecutive amino acid residues, PKK/RPAGRxKFxETRHP and DSAWR, which bracket the AP2/EREBP DNA binding domains of the proteins and distinguish them from other members of the AP2/EREBP protein family. (Jaglo et al. (2001))

Transcription factors mediate cellular responses and control traits through altered expression of genes containing cis-acting nucleotide sequences that are targets of the introduced transcription factor. It is well appreciated in the art that the effect of a transcription factor on cellular responses or a cellular trait is determined by the particular genes whose expression is either directly or indirectly (e.g., by a cascade of transcription factor binding events and transcriptional changes) altered by transcription factor binding. In a global analysis of transcription comparing a standard condition with one in which a transcription factor is overexpressed, the resulting transcript profile associated with transcription factor overexpression is related to the trait or cellular process controlled by that transcription factor. For example, the PAP2 gene and other genes in the MYB family have been shown to control anthocyanin biosynthesis through regulation of the expression of genes known to be involved in the anthocyanin biosynthetic pathway (Bruce et al. (2000); and Borevitz et al. (2000)). Further, global transcript profiles have been used successfully as diagnostic tools for specific cellular states (e.g., cancerous vs. non-cancerous; Bhattacharjee et al. (2001); and Xu et al. (2001)). Consequently, it is evident to one skilled in the art that similarity of transcript profile upon overexpression of different transcription factors would indicate similarity of transcription factor function.

Polypeptides and Polynucleotides of the Invention

The present invention provides, among other things, transcription factors (TFs), and transcription factor homolog polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides, or novel sequence variant polypeptides or polynucleotides encoding novel variants of transcription factors derived from the specific sequences provided in the Sequence Listing. Also provided are methods for modifying a plant's biomass by modifying the size or number of leaves or seed of a plant by controlling a number of cellular processes, and for increasing a plant's resistance or tolerance to disease or abiotic stresses, respectively. These methods are based on the ability to alter the expression of critical regulatory molecules that may be conserved between diverse plant species. Related conserved regulatory molecules may be originally discovered in a model system such as Arabidopsis and homologous, functional molecules then discovered in other plant species. The latter may then be used to confer increased biomass, disease resistance or abiotic stress tolerance in diverse plant species.

Exemplary polynucleotides encoding the polypeptides of the invention were identified in the Arabidopsis thaliana GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. In addition, further exemplary polynucleotides encoding the polypeptides of the invention were identified in the plant GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. Polynucleotide sequences meeting such criteria were confirmed as transcription factors.

Additional polynucleotides of the invention were identified by screening Arabidopsis thaliana and/or other plant cDNA libraries with probes corresponding to known transcription factors under low stringency hybridization conditions. Additional sequences, including full length coding sequences, were subsequently recovered by the rapid amplification of cDNA ends (RACE) procedure using a commercially available kit according to the manufacturer's instructions. Where necessary, multiple rounds of RACE are performed to isolate 5′ and 3′ ends. The full-length cDNA was then recovered by a routine end-to-end polymerase chain reaction (PCR) using primers specific to the isolated 5′ and 3′ ends. Exemplary sequences are provided in the Sequence Listing.

Many of the sequences in the Sequence Listing, derived from diverse plant species, have been ectopically expressed in overexpressor plants. The changes in the characteristic(s) or trait(s) of the plants were then observed and found to confer increased disease resistance, increase biomass and/or increased abiotic stress tolerance. Therefore, the polynucleotides and polypeptides can be used to improve desirable characteristics of plants.

The polynucleotides of the invention were also ectopically expressed in overexpressor plant cells and the changes in the expression levels of a number of genes, polynucleotides, and/or proteins of the plant cells observed. Therefore, the polynucleotides and polypeptides can be used to change expression levels of a genes, polynucleotides, and/or proteins of plants or plant cells.

The data presented herein represent the results obtained in experiments with transcription factor polynucleotides and polypeptides that may be expressed in plants for the purpose of reducing yield losses that arise from biotic and abiotic stress.

The G482 Clade, Including G481 and Related Sequences

G481 (SEQ ID NOs: 9 and 10; AT2G38880; also known as HAP3A and NF-YB1) from Arabidopsis is a member of the HAP3/NF-YB sub-group of the CCAAT binding factor family (CCAAT) of transcription factors (FIG. 2).

Structural features and assembly of the NF-Y subunits. NF-Y is one of the most heavily studied transcription factor complexes and an extensive literature has accumulated regarding its structure, regulation, and putative roles in various different organisms. Each of the three subunits comprises a region which has been evolutionarily conserved (Li et al. (1992); Mantovani (1999)). In the NF-YA subunits, this conserved region is at the C-terminus, in the NF-YB proteins it is centrally located, and in the NF-YC subunits it is at the N-terminus. The NF-YA and NF-YC subunits also have regions which are rich in glutamine (Q) residues that also show some degree of conservation; these Q-rich regions have an activation domain function. In fact it has been shown that NF-Y contains two transcription activation domains: a glutamine-rich, serine-threonine-rich domain present in the CBF-B (HAP2, NF-YA) subunit and a glutamine-rich domain in the CBF-C(HAP5, CBF-C) subunit (Coustry et al. (1995); Coustry et al. (1996); Coustry et al. (1998); Coustry et al. (2001)).

The NF-YB and NF-YC subunits bear some similarity to histones; the conserved regions of both these subunits contain a histone fold motif (HFM), which is an ancient domain of about 65 amino acids. The HFM has a high degree of structural conservation across all histones and comprises three or four α-helices (four in the case of the NF-Y subunits) which are separated by short loops (L)/strand regions (Arents and Moudrianakis (1995)). In the histones, this HFM domain mediates dimerization and formation of non sequence-specific interactions with DNA (Arents and Moudrianakis (1995)).

Considerable knowledge has now accumulated regarding the biochemistry of NF-Y subunit association and DNA binding. The NF-YB-NF-YC subunits first form a tight dimer, which offers a complex surface for NF-YA association. The resulting trimer can then bind to DNA with high specificity and affinity (Kim and Sheffrey (1990); Bi et al. (1997); Mantovani (1999)). In addition to the NF-Y subunits themselves, a number of other proteins have been implicated in formation of the complex (Mantovani (1999)).

HAP3 (NF-YB) proteins have a modular structure and are comprised of three distinct domains: an amino-terminal A domain, a central B domain and a carboxy-terminal C domain. There is very little sequence similarity between HAP3 proteins within the A and C domains suggesting that those regions could provide a degree of functional specificity to each member of the HAP3 subfamily. The B domain is a highly conserved region that specifies DNA binding and subunit association. Lee et al. (2003) performed an elegant series of domain swap experiments between the LEC1 and a non-LEC1 like HAP3 protein (At4g14540, G485) to demonstrate that the B domain of LEC1 is necessary and sufficient, within the context of the rest of the protein, to confer its activity in embryogenesis. Furthermore, these authors identified a specific defining residue within the B domain (Asp-55) that is required for LEC1 activity and which is sufficient to confer LEC1 function to a non-LEC1 like B domain.

In FIGS. 3A-3B, HAP3 protein B domains from Arabidopsis, soybean, rice and corn are aligned with G481.

G634, the G634 Clade, and Related Sequences

G634 (SEQ ID NO: 505) encodes a TH family protein (SEQ ID NO: 506). This gene was initially identified from public partial cDNAs sequences for GTL1 and GTL2 which are splice variants of the same gene (Smalle et al (1998)). The published expression pattern of GTL1 shows that G634 is highly expressed in siliques and not expressed in leaves, stems, flowers or roots.

G1073, the G1073 Clade, and Related Sequences

G1073 (SEQ ID NO: 18, encoded by SEQ ID NO: 17) is a member of the At-hook family of transcription factors. We have now designated this locus as HERCULES 1 (HRC1), in recognition of the increased organ size seen in 35S::G1073 lines.

G1073 contains a single typical AT-hook DNA-binding motif (RRPRGRPAG; SEQ ID NO: 2382) at amino acids 63 to 71. A highly conserved 129 AA domain, with unknown function, can be identified in the single AT-hook domain subgroup. Comprised within this “second conserved domain is the DUF296 domain, which in G1073 occupies amino acids 90-209. According to the National Center for Biotechnology Information (NCBI; www.ncbi.nlm.nih.gov/), “[t]his putative domain is found in proteins that contain AT-hook motifs pfam02178, which strongly suggests a DNA-binding function for the proteins as a whole, however the function of this domain is unknown”. Following the second conserved domain, a potential acidic domain spans from position 200 to 219. Additionally, analysis of the protein using PROSITE reveals three potential protein kinase C phosphorylation sites at Ser61, Thr112 and Thr131, and three potential casein kinase II phosphorylation sites at Ser35, Ser99 and Ser276. Additional structural features of G1073 include 1) a short glutamine-rich stretch in the C-terminal region distal to the conserved acidic domain, and 2) possible PEST sequences in the same C-terminal region.

The G1073 clade generally comprises the consensus sequence:

(SEQ ID NO: 5107) RPRGRPXG, or Arg-Pro-Arg-Gly-Arg-Pro-Xaa-Gly

where X or Xaa can be any of a number of amino acid residues; in the examples that have thus far been shown to confer abiotic stress tolerance, Xaa has been shown to represent an alanine, leucine, proline, or serine residue.

Also within the G1073 clade, a second conserved domain exists that generally comprises the consensus sequence: Pro-(Xaa)₅-Leu-(Xaa)₂-Tyr (SEQ ID NO: 5108), or alternatively Pro-(Xaa)₅-Leu-(Xaa)₂-Phe (SEQ ID NO: 5109). The tenth position of these latter two sequences is an aromatic residue, specifically tyrosine or phenylalanine, in the G1073 clade sequences that have thus far been examined.

Thus, the AT-hook family transcription factors of the invention each possess an AT-hook domain and a second conserved domain, and include paralogs and orthologs of G1073 found by BLAST analysis, as described below. The AT-hook domains of G1073 and related sequences examined thus far are at least 85% identical to the At-Hook domains of G1073, and the second conserved domains of these related sequences are at least 61% identical to the second conserved domain found in G1073. These transcription factors rely on the binding specificity of their AT-hook domains; many have been shown to have similar or identical functions in plants by increasing the size and biomass of a plant.

Role of At-hook proteins. The At-hook is a short, highly-conserved, DNA binding protein motif that comprises a conserved nine amino acid peptide (Nieto-Sotelo, Ichida and Quail (1994)), the seminal domain of which contains KRPRGRPKK (SEQ ID NO: 5110; Reeves and Nissen, 1990) and is capable of binding to the minor groove of DNA (Reeves and Nissen (1990)). At the center of this AT-hook motif is a short, strongly conserved tripeptide (GRP) comprised of glycine-arginine-proline (Aravind and Landsman (1998)). At-hook motifs were first recognized in the non-histone chromosomal protein HMG-I(Y) but have since been found in other DNA binding proteins from a wide range of organisms. In general, it appears that the AT-hook motif is an auxiliary protein motif cooperating with other DNA-binding activities and facilitating changes in the structure of the chromatin (Aravind and Landsman (1998)). The AT-hook motif can be present in a variable number of copies (1-15) in a given AT-hook protein. For example, the mammalian HMG-I(Y) proteins have three copies of this motif.

Overexpression of G1073 in Arabidopsis. We established that overexpression of G1073 leads to increased vegetative biomass and seed yield compared to control plants. As a result of these phenotypes we assigned the gene name HERCULES1 (HRC1) to G1073. Drought tolerance was observed in 35S::G1073 transgenic lines. We have also observed hyperosmotic stress-tolerance phenotypes, such as tolerance to high salt and high sucrose concentrations, in plate-based assays performed on 35S::G1073 plants.

Due to increased cell size and number, 35S::G1073 Arabidopsis lines display enlarged organs. We also conducted some preliminary analyses into the basis of the enhanced biomass of 35S::G1073 Arabidopsis lines. We found that the increased mass of 35S::G1073 transgenic plants could be attributed to enlargement of multiple organ types including leaves, stems, roots and floral organs. Petal size in the 35S::G1073 lines was increased by 40-50% compared to wild type controls. Petal epidermal cells in those same lines were approximately 25-30% larger than those of the control plants. Furthermore, we found 15-20% more epidermal cells per petal, compared to wild type. Thus, at least in petals, the increase in size was associated with an increase in cell size as well as in cell number. Additionally, images from the stem cross-sections of 35S::G1073 plants revealed that cortical cells were large and that vascular bundles contained more cells in the phloem and xylem relative to wild type.

Advantages of Root-Specific Expression:

Plants often respond to stresses such as limited water or nutrients by altering their root-shoot ratios, root architecture, or root growth. These changes are mediated through transcriptional responses in both the root and shoot. Since there is evidence that G1073 has a native role in the root, this gene and other genes encoding related proteins from the plant At-hook family may confer drought tolerance by controlling root development or other root responses. Root specific expression of G1073 and other sequences that encode plant At-hook proteins (for example: G1067, G1069, G1073, G1075, G1076, G1667, G1945, G2153, G2155, G2156, G2157, G3399, G3400, G3401, G3406, G3407, G3408, G3456, G3459, G3460, G3556, G597, G605, G1068, G1128, G1399, G1944, G2522 (SEQ ID NOs: 798, 802, 18, 804, 806, 1116, 28, 1420, 1422, 1424, 1426, 1870, 1872, 1874, 5145, 1876, 1878, 1916, 1918, 1920, 2034, 476, 484, 800, 828, 964, 1286, 1552) under the regulatory control of a promoter that drives root specific or root enhanced expression, such as, for example, ARSK1, NAS2, or others [such as the regulator regions from genes discussed recently by Birnbaum et al. (2003) or Brady et al. (2007) as having root specific expression patterns], may be used to produce transformed plants that are water deficit tolerant but lack undesirable developmental effects that may be associated with constitutive overexpression (e.g., for some applications, large plants, or changes in plant organ size or shape may be disadvantageous).

G682, the G682 Clade, and Related Sequences

We identified G682, SEQ ID NO: 550, as a transcription factor from the Arabidopsis BAC AF007269 based on sequence similarity to other members of the MYB-related family within the conserved domain. The gene corresponds to At4G01060, annotated by the Arabidopsis Genome initiative. G682 is member of a clade of related proteins that range in size from 75 to 112 amino acids. These proteins contain a single MYB repeat, which is not uncommon for plant MYB transcription factors. Information on gene function has been published for four of the genes in this clade, CAPRICE (CPC/G225), TRIPTYCHON (TRY/G1816), ENHANCER of TRY and CPC 1 (ETC1/G2718) and ENHANCER of TRY and CPC 2 (ETC2/G226). Members of the G682 clade were found to promote epidermal cell type alterations when overexpressed in Arabidopsis. These changes include both increased numbers of root hairs compared to wild type plants, as well as a reduction in trichome number. In addition, overexpression lines for the first five members of the clade showed a reduction in anthocyanin accumulation in response to stress, and enhanced tolerance to hyperosmotic stress. In the case of 35S::G682 transgenic lines, an enhanced tolerance to high heat conditions was also observed.

MYB (Myeloblastosis) transcription factors. MYB proteins are functionally diverse transcription factors found in both plants and animals. They share a signature DNA-binding domain of approximately 50 amino acids that contains a series of highly conserved residues with a characteristic spacing (Graf (1992)). Critical in the formation of the tertiary structure of the conserved Myb motif is a series of consistently spaced tryptophan residues (Frampton et al. (1991)). Animal Mybs contain three repeats of the Myb domain: R1, R2, and R3. Plant Mybs usually contain two imperfect Myb repeats near their amino termini (R2 and R3), although there is a small subgroup of three repeat (R1R2R3) mybs similar to those found in animals, numbering approximately eight in the Arabidopsis genome. A subset of plant Myb-related proteins contain only one repeat (Martin and Paz-Ares (1997)). Each Myb repeat has the potential to form three alpha-helical segments, resembling a helix-turn-helix structure (Frampton et al. (1991)). Although plant Myb proteins share a homologous Myb domain, differences in the overall context of their Myb domain and in the specific residues that contact the DNA produce distinct DNA-binding specificities in different members of the family. Once bound, MYB proteins function to facilitate transcriptional activation or repression, and this sometimes involves interaction with a protein partner (Goff et al. (1992)). MYB transcription factors are divided into two families; the MYB (R1)R2R3 family which contains transcription factors that typically have two imperfect MYB repeats, and the MYB-related family which contains transcription factors that contain a single MYB-DNA binding motif.

The MYB-related family (Single-repeat MYB transcription factors). There are approximately 50 members of this family in Arabidopsis. The MYB-related DNA-binding domain contains approximately 50 amino acids with a series of highly conserved residues arranged with a characteristic spacing. The single-repeat MYB proteins do not contain a typical transcriptional activation domain and this suggests that they may function by interfering with the formation or activity of transcription factors or transcription factor complexes (Wada et al. (1997); Schellmann et al. (2002)). In addition to the G682 clade, two well characterized transcription factors, CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) represent additional well-characterized MYB-related proteins that contain single MYB repeats (Wang et al. (1997); Schaffer et al. (1998)).

Protein structure and properties. G682 and its paralogs and orthologs are composed (almost entirely) of a single MYB-repeat DNA binding domain that is highly conserved across plant species. An alignment of the G682-like protein MYB domains from Arabidopsis, soybean, rice and corn that are being analyzed is shown in FIG. 5.

Because the G682 clade members are short proteins that are comprised almost exclusively of a DNA binding motif, it is likely that they function as repressors. This is consistent with in expression analyses indicating that CPC represses its own transcription as well as that of WER and GL2 (Wada et al. (2002); Lee and Schiefelbein (2002)). Repression may occur at the level of DNA binding through competition with other factors at target promoters, although repression via protein-protein interactions cannot be excluded.

G867, the G867 Clade, and Related Sequences

We first identified G867, SEQ ID NO: 16, encoded by SEQ ID NO: 15, as a transcription factor encoded by public EST sequence (GenBank accession N37218). Kagaya et al. (Kagaya et al. (1999)) later assigned the gene the name Related to ABI3/VP1 I (RAV1) based on the presence of a B3 domain in the C-terminal portion of the encoded protein. In addition to the B3 domain, G867 contains a second DNA binding region, an AP2 domain, nearer to its N terminus. There are a total of six RAV related proteins with this type of structural organization in the Arabidopsis genome: G867 (AT1G13260, RAV1), G9 (AT1G68840, which has been referenced as both RAP2.8, Okamuro et al. (1997), and as RAV2, Kagaya et al. (1999)), G1930, SEQ ID NO: 1275 (AT3G25730), G993, SEQ ID NO: 745 (AT1G25560), AT1G50680 and AT1G51120. Recently, G867 was identified by microarray as one of 53 genes down-regulated by brassinosteroids in a det2 (BR-deficient) cell culture. This down-regulation was not dependent on BR11, and mild down-regulation of G867 also occurred in response to cytokinins (Hu et al. (2004). These authors also showed that overexpression of G867 reduces both root and leaf growth, and causes a delay in flowering. A G867 knockout displays early flowering time, but no other obvious effect. A detailed genetic characterization has not been published for any of the other related genes.

On the basis of the AP2 domain, the six RAV-like proteins were categorized as part of the AP2 family. It should be noted, however, that the B3 domain is characteristic of proteins related to ABI3/VP1 (Suzuki et al. (1997)).

Protein structure and properties. G867 lacks introns and encodes a 344 amino acid protein with a predicted molecular weight of 38.6 kDa. Analysis of the binding characteristics of RAV1 (G867) revealed that the protein binds as a monomer to a bipartite target consisting of a “CAACA motif” and a “CACCTG motif” which can be separated by 2-8 nucleotides, and can be present in different relative orientations (Kagaya et al. (1999)). Gel shift analysis using different deletion variants of RAV1 have shown that the AP2 domain recognizes the “CAACA” motif while the B3 domain interacts with the CACCTG sequence. Although both binding domains function autonomously, the affinity for the target DNA is greatly enhanced when both domains are present (Kagaya et al. (1999)), suggesting that the target DNA can act as an allosteric effector (Lefstin and Yamamoto (1998)).

AP2 DNA binding domain. The AP2 domain of G867 is localized in the N-terminal region of the protein. The “CAACA element” recognized by G867 differs from the “GCCGCC motif” present in ERF (ethylene response factors, Hao et al. (1998); Hao et al. (2002)) target promoters, and from the “CCGAC motif” involved in regulation of dehydration responsive genes by the CBF/DREB1 and DREB2 group of transcription factors (Sakuma et al. (2002)). In case of the CBF proteins, regions flanking the AP2 domain are very specific and are not found in other Arabidopsis transcription factors. Furthermore, those regions are highly conserved in CBF proteins across species (Jaglo et al. (2001)). The regions flanking the AP2 domain are also highly conserved in G867 and the paralogs G9, G1930, and G993 (SEQ ID NOs: 16, 44, 1276 and 746, respectively).

B3 DNA binding domain. The B3 domain is present in several transcription factor families: RAV, ABI3/VP1, and ARF. It has been shown for all three families that the B3 domain is sufficient for DNA binding (Table 1). However, the binding specificity varies significantly. These differences in target specificity are also reflected at the protein level. Although all B3 domains share certain conserved amino acids, there is significant variation between families. The B3 domain of the RAV proteins G867 (RAV1), G9 (RAV2), G1930, and G993 is highly conserved, and substantially more closely related to the ABI3 than to the ARF family. Despite the fact that the B3 domain can bind DNA autonomously (Kagaya et al. (1999); Suzuki et al. (1997)), in general, B3 domain transcription factors interact with their targets via two DNA binding domains (Table 1). In case of the RAV and ABI3 family, the second domain is located on the same protein. It has been shown for ABI3 (G621) that cooperative binding increases not only the specificity but also the affinity of the interaction (Ezcurra et al. (2000)).

TABLE 1 Binding sites for different B3 domains 2nd Domain present Family Binding site Element in protein Reference RAV CACCTG — AP2 Kagaya et al. (1997) ABI3 CATGCATG RY/G-box B2 Ezcurra et al. (2000) ARF TGTCTC AuxRE other TxF Ulmasov et al. (1997)

Other protein features. A potential bipartite nuclear localization signal has been identified in the G867 protein. A protein scan also revealed several potential phosphorylation sites.

Examination of the alignment of only those sequences in the G867 clade (having monocot and eudicot subnodes), indicates 1) a high degree of conservation of the AP2 domains in all members of the clade, 2) a high degree of conservation of the B3 domains in all members of the clade; and 3) a high degree of conservation of an additional motif, the DML motif found between the AP2 and B3 domains in all members of the clade: (H/R S K Xa E/G I/V V D M L R K/R H T Y Xa E/D/N E L/F Xa O/H S/N/R/G (SEQ ID NO: 5111), constituting positions 135-152 in G867 (SEQ ID NO: 16). As a conserved motif found in G867 and its paralogs, the DML motif was used to identify additional orthologs of SEQ ID NO: 16. A significant number of sequences were found that had a minimum of 71% identity to the 22 residue DML motif of G867. The DML motif between the AP2 and B3 DNA binding domain is predicted to have a particularly flexible structure. This could explain the observation that binding of the bipartite motif occurs with similar efficiency, irrespective of the spacing and the orientation of the two motifs (the distance between both elements can vary from 2-8 bp, Kagaya et al. (1999)). Importantly, the DML motif located between the AP2 domain and the B3 domain is not conserved between the G867 clade and other RAV polypeptides that have been examined. This motif presumably has a role in determining the unique function of the G867 clade of RAV-like proteins.

G28, the G28 Clade, and Related Sequences

G28 (SEQ ID NO: 2, encoded by SEQ ID NO: 1) corresponds to AtERF1 (GenBank accession number AB008103) (Fujimoto et al. (2000)). G28 appears as gene At4 g17500 in the annotated sequence of Arabidopsis chromosome 4 (AL161546.2). G28 has been shown to confer resistance to both necrotrophic and biotrophic pathogens. The G28 polypeptide (SEQ ID NO: 2) is a member of the B-3a subgroup of the ERF subfamily of AP2 transcription factors, defined as having a single AP2 domain and having specific residues in the DNA binding domain that distinguish this large subfamily (65 members) from the DREB subfamily (see below). AtERF1 is apparently orthologous to the AP2 transcription factor Pti4, identified in tomato, which has been shown by Martin and colleagues to function in the Pto disease resistance pathway, and to confer broad-spectrum disease resistance when overexpressed in Arabidopsis (Zhou et al. (1997); Gu et al. (2000); Gu et al. (2002)).

AP2 domain transcription factors. This large transcription factor gene family includes 145 transcription factors (Weigel (1995); Okamuro et al. (1997); Riechmann and Meyerowitz (1998); Riechmann et al. (2000)). Based on the results of our earlier genomics screens it is clear that this family of proteins affect the regulation of a wide range of morphological and physiological processes, including the acquisition of abiotic and biotic stress tolerance. The AP2 family includes the AP2/ERF group which contain a single AP2 domain. This AP2/ERF class can be further categorized into three subgroups:

The DREB (“A”) (dehydration responsive element binding) sub-family which comprises 56 genes. Many of the DREBs are involved in regulation of abiotic stress tolerance pathways (Stockinger et al. (1997); Jaglo-Ottosen et al. (1998); Finkelstein et al. (1998); Sakuma et al. (2002)).

The ERF (ethylene response factor) sub-family (“B”) which includes 65 genes, several of which are involved in regulation of biotic stress tolerance pathways (Ohme-Takagi and Shinshi (1995); Zhou et al. (1997)). The DREB and ERF sub-groups are distinguished by the amino acids present at position 14 and 19 of the AP2 domain: while DREBs are characterized by Val-14 and Glu-19, ERFs typically have Ala-14 and Asp-19. Recent work indicates that those two amino acids have a key function in determining the target specificity (Sakuma et al. (2002), Hao et al. (2002)).

[3] The RAV class (6 genes) all of which have a B3 DNA binding domain in addition to the AP2 DNA binding domain, and which also regulate abiotic stress tolerance pathways.

The role of ERF transcription factors in stress responses: ERF transcription factors in disease resistance. The first indication that members of the ERF group might be involved in regulation of plant disease resistance pathways was the identification of Pti4, Pti5 and Pti6 as interactors with the tomato disease resistance protein Pto in yeast 2-hybrid assays (Zhou et al. (1997)). Since that time, many ERF genes have been shown to enhance disease resistance when overexpressed in Arabidopsis or other species. These ERF genes include ERF1 (G 1266) of Arabidopsis (Berrocal-Lobo et al. (2002); Berrocal-Lobo and Molina, (2004)); Pti4 (Gu et al. (2002)) and Pti5 (He et al. (2001)) of tomato; Tsi1 (Park et al. (2001); Shin et al. (2002)), NtERF5 (Fischer and Droge-Laser (2004)), and OPBP1 (Guo et al. (2004)) of tobacco; CaERFLP1 (Lee et al. (2004)) and CaPF1 (Yi et al. (2004)) of hot pepper; and AtERF1 (G28) and TDR1 (G1792) of Arabidopsis (our data).

Protein structure and properties. G28 lacks introns and encodes a 266 amino acid protein with a predicted molecular weight of 28.9 kDa. Specific conserved motifs have been identified through alignments with other related ERFs. The AP2 domain of G28 is relatively centrally positioned in the intact protein. G28 has been shown to bind specifically to the AGCCGCC motif (GCC box: Hao et al. (1998); Hao et al. (2002)). Our analysis of the G28 regulon by global transcript profiling is consistent with this, as the 5′ regions of genes up-regulated by G28 are enriched for the presence of AGCCGCC motifs. The AP2 domain of AtERF1 (G28) was purified and used by Allen et al. (1998) in solution NMR studies of the AP2 domain and its interaction with DNA. This analysis indicated that certain residues in three beta-strands are involved in DNA recognition, and that an alpha helix provides structural support for the DNA binding domain.

A potential bipartite nuclear localization signal has been reported in the G28 protein. A protein scan also revealed several potential phosphorylation sites, but the conserved motifs used for those predictions are small, have a high probability of occurrence. However, the orthologous Pti4 sequence has been shown to be phosphorylated in multiple locations, which have yet to be mapped in detail. A protein alignment of closely related ERF sequences indicates the presence of conserved domains unique to B-3a ERF proteins. For example, a motif not found in other Arabidopsis transcription factors is found directly C-terminal to the AP2 domain in eudicot sequences, but is not found in monocot sequences. Another conserved motif is found 40-50 amino acids N-terminal to the AP2 DNA binding domain. The core of this motif is fairly well conserved in both eudicots and monocots, but extensions of the motif are divergent between eudicots and monocots. The identification of specific motifs unique to small clades of ERF transcription factors suggests that these motifs may be involved in specific interactions with other protein factors involved in transcriptional control, and thereby may determine functional specificity. Known transcriptional activation domains are either acidic, proline rich or glutamine rich (Liu et al. (1999)). The G28 protein contains one acid-enriched region (overlapping with the first eudicot-specific motif). There is also evidence that regions rich in serine, threonine, and proline may function in transcriptional activation (Silver et al. (2003)). There are two ser/pro-enriched regions in the region N-terminal to the AP2 domain. None of these domains has yet to be demonstrated directly to have a role in transcriptional activation.

G1792, the G1792 Clade, and Related Sequences

We first identified G1792 (AT3G23230; SEQ ID NO: 23, 24) as a transcription factor in the sequence of BAC clone K14B15 (AB025608, gene K14B15.14). We have assigned the name TRANSCRIPTIONAL REGULATOR OF DEFENSE RESPONSE 1 (TDR1) to this gene, based on its apparent role in disease responses. The G1792 protein contains a single AP2 domain and belongs to the ERF class of AP2 proteins. A review of the different sub-families of proteins within the AP2 family is provided in the information provided for G28, above. The G28 disclosure provided herein includes description of target genes regulated by ERF transcription factors, the role of ERF transcription factors in stress responses: ERF transcription factors in disease resistance, ERF transcription factors in abiotic stress responses, regulation of ERF transcription factors by pathogen and small molecule signaling, etc., which also pertain to G1792.

G1792 overexpression increases survivability in a soil-based drought assay. 35S::G1792 lines exhibited markedly enhanced drought tolerance in a soil-based drought screen compared to wild-type, both in terms of their appearance at the end of the drought period, and in survival following re-watering.

G1792 overexpression increases tolerance to growth on nitrogen-limiting conditions. 35S::G1792 transformants showed more tolerance to growth under nitrogen-limiting conditions. In a root growth assay under conditions of limiting N, 35S::G1792 lines were slightly less stunted. In an germination assay that monitors the effect of carbon on nitrogen signaling through anthocyanin production (with high sucrose +/−glutamine; Hsieh et al. (1998)), the 35S::G1792 lines made less anthocyanin on high sucrose (+glutamine), suggesting that the gene could be involved in the plant's ability to monitor carbon and nitrogen status.

G1792 overexpression causes morphological alterations. Plants overexpressing G1792 showed several mild morphological alterations: leaves were darker green and shiny, and plants bolted, and subsequently senesced, slightly later than wild-type controls. Among the T1 plants, additional morphological variation (not reproduced later in the T2 plants) was observed: many showed reductions in size as well as aberrations in leaf shape, phyllotaxy, and flower development.

G1792 overexpression produces disease resistance. 35S::G1792 plants were more resistant to the fungal pathogens Fusarium oxysporum and Botrytis cinerea: they showed fewer symptoms after inoculation with a low dose of each pathogen. This result was confirmed using individual T2 lines. The effect of G1792 overexpression in increasing resistance to pathogens received further, incidental confirmation. T2 plants of 35S::G1792 lines 5 and 12 were being grown (for other purposes) in a room that suffered a serious powdery mildew infection. For each line, a pot of 6 plants was present in a flat containing 9 other pots of lines from unrelated genes. In either of the two different flats, the only plants that were free from infection were those from the 35S::G1792 line. This observation suggested that G1792 overexpression increased resistance to powdery mildew.

G1792 has three paralogs, G30, G1791 and G1795 (SEQ ID NO: 66, 1172 and 26, respectively), which were not assayed for disease resistance in an earlier genomics program because their overexpression caused severe negative side effects. Some evidence suggested that these genes might play a role in disease resistance: expression of G1795 and G1791 was induced by Fusarium, and G1795 by salicylic acid, in RT-PCR experiments, and the lines shared the glossy phenotype observed for G1792. Phylogenetic trees based on whole protein sequences do not always make the relationship of these proteins to G1792 clear; however, the close relationship of these proteins is evident in an alignment and in a phylogenetic analysis based on the conserved AP2 domain and a second conserved motif, the EDLL domain described below.

In this study G1792, G1791, G1795 and G30 were expressed under the control of four different promoters using the two-component system. The promoters chosen were 35S, RBCS3 (mesophyll or photosynthetic-specific), LTP1 (epidermal-specific), and 35S::LexA:GAL4:GR (dexamethasone-inducible). All promoters other than 35S produced substantial amelioration of the negative side effects of transcription factor overexpression.

Five lines for each combination were tested with Sclerotinia, Botryis, or Fusarium. Interestingly, G1791 and G30 conferred significant resistance to Sclerotinia when expressed under RBCS3 or 35S::LexA:GAL4:GR, even though G1792 does not confer Sclerotinia resistance. These results support the hypothesis that genes of this clade confer disease resistance when expressed under tissue specific or inducible promoters.

TABLE 2 Disease screening of G1792 and paralogs under different promoters G1792 G1791 G1795 G30 SEQ ID NO: 24 1172 26 66 B S F B S F B S F B S F 35S ++ wt + nd nd nd nd nd nd nd nd nd RBCS3 + wt + wt wt wt ++ ++ wt + + wt LTP1 wt wt nd + wt wt ++ + wt + wt wt 35S, ++ wt + ++ ++ wt ++ ++ wt ++ ++ wt Dexametha- sone-induced Abbreviations and symbols: B, Botrytis S, Sclerotinia F, Fusarium Scoring: wt, wild-type (susceptible) phenotype +, mild to moderate resistance ++, strong resistance nd, not determined

Domains. In addition to the AP2 domain (domains of G1792 clade members are shown in Table 23), G1792 contains a putative activation domain. This domain has been designated the “EDLL domain” based on four amino acids that are highly conserved across paralogs and orthologs of G11792 (FIG. 19).

Tertiary Structure. The solution structure of an ERF type transcription factor domain in complex with the GCC box has been determined (Allen et. al., 1998). It consists of a β-sheet composed of three strands and an β-helix. Flanking sequences of the AP2 domain of this protein were replaced with the flanking sequences of the related CBF1 protein, and the chimeric protein was found to contain the same arrangement of secondary structural elements as the native ERF type protein (Allen et al. (1998)). This implies that the secondary structural motifs may be conserved for similar ERF type transcription factors within the family.

DNA Binding Motifs. Two amino acid residues in the AP2 domain, Ala-14 and Asp-19, are definitive of the ERF class transcription factors Sakuma et al. (2002). Recent work indicates that these two amino acids have a key function in determining binding specificity (Sakuma et al. (2002), Hao et al. (2002)) and interact directly with DNA. The 3-dimensional structure of the GCC box complex indicates the interaction of the second strand of the β-sheet with the DNA.

G47, the G47 Clade, and Related Sequences

G47 (SEQ ID NO: 5, AT1G22810) encodes a member of the AP2 class of transcription factors (SEQ ID NO: 6) and was included based on the resistance to drought-related abiotic stress exhibited by 35S::G47 Arabidopsis lines and by overexpression lines for the closely related paralog, G2133 (SEQ ID NO: 7 and polypeptide SEQ ID NO: 8, AT1G71520). A detailed genetic characterization has not been reported for either of these genes in the public literature.

AP2 family transcription factors. Based on the results of our earlier genomics screens, it is clear that this family of proteins affect the regulation of a wide range of morphological and physiological processes, including the acquisition of stress tolerance. The AP2 family can be further divided into subfamilies as detailed in the G28 section, above.

G47 and G2133 protein structure. G47 and G2133 and other highly related AP2 proteins (FIG. 14) and are members of the AP2/ERF subfamily. Both proteins possess an AP2 domain at the amino terminus and a somewhat acidic region at the C-terminus that might constitute an activation domain. A putative bipartite NLS is located at the start of the AP2 domain in both proteins. Sakuma et al. (Sakuma et al. (2002)) categorized these factors within the A-5 class of the DREB related sub-group based on the presence of a V residue at position 14 within the AP2 domain. Importantly, however, position 19 within the AP2 domain is occupied by a V residue in both G2133 and G47, rather than an E residue, as is the case in the majority of DREBs. Additionally, the “RAYD-box” within the AP2 domains of these two proteins is uniquely occupied by the sequence that substitutes a “V” for the “R” and an “H” for the “Y” in the RAYD-box (within SEQ ID NO: 2375, and near the right margin of the top group of subsequences in FIG. 14), a combination not found in any other Arabidopsis AP2/ERF protein (Sakuma et al. (2002)). These differences to other AP2 proteins could confer unique DNA binding properties on G2133 and G47.

Morphological effects of G47 and G2133 overexpression. A number of striking morphological effects were observed in 35S::G47 lines. At early stages, the plants were somewhat reduced in size. However, these lines flowered late and eventually developed an apparent increase in rosette size compared to mature wild-type plants. Additionally, the 35S::G47 plants showed a marked difference in aerial architecture; inflorescences displayed a short stature, had a reduction in apical dominance, and developed thick fleshy stems. When sections from these stems were stained and examined, it was apparent that the vascular bundles were grossly enlarged compared to wild-type. Similar morphological changes were apparent in shoots of 35S::G2133 lines, but most of the 35S::G2133 lines exhibited much more severe dwarfing at early stages compared to 35S::G47 lines. Nevertheless, at later stages, a number of 35S::G2133 lines showed a very similar reduction of apical dominance and a fleshy appearance comparable to that seen in 35S::G47 lines.

Physiological effects of G47 and G2133 overexpression. Both 35S::G2133 lines and 35S::G47 lines exhibited abiotic stress resistance phenotypes in the screens performed during our earlier genomics program. 35S::G47 lines displayed increased tolerance to hyperosmotic stress (PEG) whereas 35S::G2133 lines were more tolerant to the herbicide glyphosate compared to wild type.

The increased tolerance of 35S::G47 lines to PEG, combined with the fleshy appearance and altered vascular structure of the plants, led us to test these lines in a soil drought screen. 35S::G2133 lines were also included in that assay, given the close similarity between the two proteins and the comparable morphological effects obtained. Both 35S::G47 and 35S::G2133 lines showed a strong performance in that screen and exhibited markedly enhanced drought tolerance compared to wild-type, both in terms of their appearance at the end of the drought period, and in survivability following re-watering. In fact, of the approximately 40 transcription factors tested in that screen, 35S::G2133 lines showed the top performance in terms of each of these criteria.

G1274, the G1274 Clade, and Related Sequences

G1274 (SEQ ID NO: 19) from Arabidopsis encodes a member of the WRKY family of transcription factors (SEQ ID NO: 20) and was included based primarily on soil-based drought tolerance exhibited by 35S::G1274 Arabidopsis lines. G1274 corresponds to AtWRKY51 (At5g64810), a gene for which there is currently no published information.

WRKY transcription factors. In Arabidopsis alone, there are more than 70 members of the WRKY superfamily. The defining feature of the family is the ˜57 amino acid DNA binding domain that contains a conserved heptapeptide motif. Additionally, all WRKY proteins have a novel zinc-finger motif contained within the DNA binding domain. There are three distinct groups within the superfamily, each principally defined by the number of WRKY domains and the structure of the zinc-finger domain (reviewed by Eulgem et al. (2000)). Group I members have two WRKY domains, while Group II members contain only one. Members of the Group II family can be further split into five distinct subgroups (IIa-e) based on conserved structural motifs. Group III members have only one WRKY domain, but contain a zinc finger domain that is distinct from Group II members. The majority of WRKY proteins are Group II members, including G1274 and the related genes being studied here. An additional common feature found among WRKY genes is the existence of a conserved intron found within the region encoding the C-terminal WRKY domain of group I members or the single WRKY domain of group II/III members. In G1274, this intron occurs between the sequence encoding amino acids R130 and N131.

Structural features of G1274. The G1274 sequence possesses a potential serine-threonine-rich activation domain and putative nuclear localization signals, the “WRKY” (DNA binding) domain, and zinc finger motif, with the pattern of potential zinc ligands C—X₄₋₅—C—X₂₂₋₂₃—H-X₁-H (SEQ ID NO: 5164).

G2999, the G2999 Clade, and Related Sequences

G2999 (SEQ ID NO: 1793, AT2G18350) encodes a member of the ZF-HD class of transcription factors (SEQ ID NO: 1794) and was included based on the resistance to drought-related abiotic stress exhibited by 35S::G2999 lines.

Identification of ZF-HD transcription factors and their role in plants. The ZF-HD family of transcriptional regulators was identified by Windhovel et al. (2001). At the C-termini, a region was apparent that had many of the features of a homeodomain, whereas at the N-termini, two zinc finger motifs were present. Given the presence of zinc fingers and the potential homeodomain, Windhovel et al. (2001), named the new family of proteins as the ZF-HD group.

Using BLAST searches we have identified a variety of ZF-HD proteins from a variety of other species, including rice and corn (FIGS. 21A-21B, and FIGS. 22A-22B).

Structural features of ZF-HD proteins. G2999 comprises an acidic region at the N-terminus which might represent an activation domain and a number of motifs which might act as nuclear localization signals.

It is well established that homeodomain proteins are transcription factors, and that the homeodomain is responsible for sequence specific recognition and binding of DNA (Affolter et al. (1990); Hayashi and Scott (1990), and references therein). Genetic and structural analysis indicate that the homeodomain operates by fitting the most conserved of three alpha helices, helix 3, directly into the major groove of the DNA (Hanes and Brent (1989); Hanes and Brent (1991); Kissinger et al. (1990); Wolberger et al. (1991); Duboule (1994)). A large number of homeodomain proteins have been identified in a range of higher plants (Burglin (1997); Burglin (1998)), and we will define these as containing the ‘classical’ type of homeodomain. These all contain the signature WFXNX[RK] (X=any amino acid, [RK] indicates either an R or K residue at this position; SEQ ID NO: 5165) within the third helix.

Data from the Genome Initiative indicate that there are around 90 “classical” homeobox genes in Arabidopsis. These are now being implicated in the control of a host of different processes. In many cases, plant homeodomains are found in proteins in combination with additional regulatory motifs such as leucine zippers. Classical plant homeodomain proteins can be broadly categorized into the following different classes based on homologies within the family, and the presence of other types of domain: KNOX class I, KNOX class II, HD-BEL1, HD-ZIP class I, HD-ZIP class II, HD-ZIP class III, HD-ZIP class IV (GL2 like), PHD finger type, and WUSCHEL-like (Freeling and Hake (1985); Vollbrecht et al. (1991); Schindler et al. (1993); Sessa et al. (1994); Kerstetter et al. (1994); Kerstetter et al. (1997); Burglin (1997); Burglin (1998); Schoof et al. (2000)). A careful examination of the ZF-HD proteins reveals a number of striking differences to other plant homeodomains. The ZF-HD proteins all lack the conserved F residue within the conserved WFXNX[RK] (X=any amino acid, [RK] indicates either an R or K residue at this position; SEQ ID NO: 5165) motif of the third helix. Additionally, there are four amino acids inserted in the loop between first and second helices of the ZF-HD proteins, whereas in other HD proteins there are a maximum of three amino acids inserted in this position (Burglin (1997)). When these homeodomains are aligned with classical homeodomains from plants, they form a very distinct clade within the phylogeny. Thus, these structural distinctions within the homeodomain could confer functional properties on ZF-HD proteins that are different to those found in other HD proteins.

The zinc finger motif at the N-terminus is highly conserved across the ZF-HD family. An alignment showing this region from the 14 Arabidopsis ZF-HD proteins and selected ZF-HD proteins from other species is shown in FIGS. 21A-21B. Yeast two-hybrid experiments performed by Windhovel et al. (2001) demonstrated that ZF-HD proteins form homo and heterodimers through conserved cysteine residues within this region.

G3086, the G3086 Clade, and Related Sequences

G3086 (SEQ ID NO: 1835 and 1836, AT1G51140) confers tolerance to drought related stress as exhibited by 35S::G3086 Arabidopsis lines. G3086 belongs to the basic/helix-loop-helix (bHLH) family of transcription factors. This family is defined by the bHLH signature domain, which consists of 60 amino acids with two functionally distinct regions. The basic region, located at the N-terminal end of the domain, is involved in DNA binding and consists of 15 amino acids with a high number of basic residues. The HLH region, at the C-terminal end, functions as a dimerization domain (Murre et al. (1989); Ferre-D'Amare et al. (1994)) and is constituted mainly of hydrophobic residues that form two amphipathic helices separated by a loop region of variable sequence and length (Nair and Burley (2000)). Outside of the conserved bHLH domain, these proteins exhibit considerable sequence divergence (Atchley et al. (1999)). Cocrystal structural analysis has shown that the interaction between the HLH regions of two separate polypeptides leads to the formation of homodimers and/or heterodimers and that the basic region of each partner binds to half of the DNA recognition sequence (Ma et al. (1994); Shimizu et al. (1997)). Some bHLH proteins form homodimers or restrict their heterodimerization activity to closely related members of the family. On the other hand, some can form heterodimers with one or several different partners (Littlewood and Evan (1998).

The core DNA sequence motif recognized by the bHLH proteins is a consensus hexanucleotide sequence known as the E-box (5′-CANNTG-3′). There are different types of E-boxes, depending on the identity of the two central bases. One of the most common is the palindromic G-box (5′-CACGTG-3′). Certain conserved amino acids within the basic region of the protein provide recognition of the core consensus site, whereas other residues in the domain dictate specificity for a given type of E-box (Robinson et al. (2000)). In addition, flanking nucleotides outside of the hexanucleotide core have been shown to play a role in binding specificity (Littlewood and Evan (1998); Atchley et al. (1999); Massari and Murre (2000)), and there is evidence that a loop residue in the protein plays a role in DNA binding through elements that lie outside of the core recognition sequence (Nair and Burley (2000)).

Protein structure. There are two important functional activities determined by the amino acid sequence of the bHLH domain: DNA binding and dimerization. The basic region in the bHLH domain determines the DNA binding activity of the protein (Massari and Murre (2000)). The DNA binding bHLH category can be subdivided further into two subcategories based on the predicted DNA binding sequence: (1) the E-box binders and (2) the non-E-box binders (Toledo-Ortiz et al. (2003)) based on the presence or absence of two specific residues in the basic region: Glu-319 and Arg-321. These residues constitute the E-box recognition motif, because they are conserved in the proteins known to have E-box binding capacity (Fisher and Goding (1992); Littlewood and Evan (1998)). The analysis of the crystal structures of USF, E47, Max, MyoD, and Pho4 (Ellenberger et al. (1994); Ferre-D'Amare et al. (1994); Ma et al. (1994); Shimizu et al. (1997); Fuji et al. (2000)) have shown that Glu-319 is critical because it contacts the first CA in the E-box DNA binding motif (CANNTG). Site-directed mutagenesis experiments with Pho4, in which other residues (Gln, Asp, and Leu) were substituted for Glu-13, demonstrated that the substitution abolished DNA binding (Fisher and Goding (1992)). Meanwhile, the role of Arg-16 is to fix and stabilize the position of the critical Glu-13; therefore, it plays an indirect role in DNA binding (Ellenberger et al. (1994); Shimizu et al. (1997); Fuji et al. (2000)).

The E-box binding bHLHs can be categorized further into subgroups based on the type of E-box recognized. Crystal structures show that the type of E-box binding preferences are established by residues in the basic region, with the best understood case being that of the G-box binders (Ellenberger et al. (1994); Ferre-D'Amare et al. (1994); Shimizu et al. (1997)). Toledo-Ortiz et al. (2003) have subdivided the Arabidopsis E-box binding bHLHs into (1) those predicted to bind G-boxes and (2) those predicted to recognize other types of E-boxes (non-G-box binders). There are three residues in the basic region of the bHLH proteins: His/Lys, Glu, and Arg at positions 315, 319, and 322 which constitute the classic G-box (CACGTG) recognition motif. Glu-319 is the key Glu involved in DNA binding, and analysis of the crystal structures of Max, Pho4, and USF indicates that Arg-322 confers specificity for CACGTG versus CAGCTG E-boxes by directly contacting the central G of the G-box. His-315 has an asymmetrical contact and also interacts with the G residue complementary to the first C in the G-box (Ferre-D'Amare et al. (1994); Shimizu et al. (1997); Fuji et al. (2000)).

Based on this analysis, G3086 is predicted to be an E-box binding protein. However, since it lacks a histidine or lysine at position 315, it is not predicted to be a G-box binding protein.

bHLH proteins are well known to dimerize, but the critical molecular determinants involved are not well defined (Shirakata et al. (1993); Littlewood and Evan (1998); Ciarapica et al. (2003)). On the other hand, the leucine residue at the position equivalent to residue 333 in G3086 has been shown to be structurally necessary for dimer formation in the mammalian Max protein (Brownlie et al. (1997)). This leucine is the only invariant residue in all bHLH proteins, consistent with a similar essential function in plant bHLH protein dimerization. Current information indicates that dimerization specificity is affected by multiple parameters, including hydrophobic interfaces, interactions between charged amino acids in the HLH region, and partner availability, but no complete explanation for partner recognition specificity has been documented (Ciarapica et al. (2003)). Thus, although empirically it seems logical that bHLH proteins most closely related in sequence in the HLH region are the most likely to form heterodimers, there has been no systematic investigation of this possibility to date.

Beyond the bHLH domain, additional functional domains have been identified in the bHLH proteins in other eukaryotes. These additional domains play roles in protein-protein interactions (e.g., PAS, WRPW, and COE in groups C, E, and F, respectively; Dang et al. (1992); Atchley and Fitch (1997); Ledent and Vervoort (2001)) and in bHLH dimerization specificity (e.g., the zipper domain, part of group B). G3086 does not appear to contain any of these functional domains apart from two nuclear localization signal (NLS) motifs. One NLS motif appears to be a simple localization signal, while the other has a bipartite structure based on the occurrence of lysine and arginine clusters.

An alignment of the full-length proteins for genes in the G3086 study group compared with a selection of other proteins from the HLH/MYC family, and a phylogenetic tree based on that alignment is shown in FIG. 23.

Abiotic stress related phenotypes. G3086 was initially included as a candidate for the drought program based on the enhanced tolerance to salt and heat exhibited by overexpression lines. 35S::G3086 lines were subsequently tested in a soil drought assay. Lines for this gene showed improved drought resistance compared to wild-type in terms of both their appearance at the end of a drought treatment and survivability to drought treatment compared to controls following re-watering.

Effects on flowering time. Lines overexpressing G3086 or G592 show a very marked acceleration in the onset of flowering. Reflecting this rapid progression through the life cycle, overexpression lines for either gene tend to have a rather spindly appearance and reduced size compared to controls.

Tables 3-33 list a number of polypeptides of the invention and include the amino acid residue coordinates for the conserved domains, the conserved domain sequences of the respective polypeptides, (sixth column); the identity in percentage terms to the conserved domain of the lead Arabidopsis sequence (the first transcription factor listed in each table), and whether the given sequence in each row was shown to confer greater biomass and yield or stress tolerance in plants (+) or has thus far not been shown to confer stress tolerance (−) for each given promoter::gene combination in our experiments. Percentage identities to the sequences listed in Tables 3-33 were determined using BLASTP analysis with defaults of wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix Henikoff & Henikoff (1992). When the conserved domain sequences found in Tables 3-33 are optimally aligned using the BLOSUM62 matrix, a gap existence penalty of 11, and a gap extension penalty of 1, similar conserved domains may be identified by virtue of having a minimum specified percentage identity. Said minimum percentage identity may be determined by the percentage identities found within a given clade of transcription factors. Examples of percentage identities to Arabidopsis sequences that are clade members are provided in Tables 3-33, although it is anticipated and expected that other percentage identities may be determined by related clade sequences to another Arabidopsis sequence, or a sequence from another plant species, where that sequence is a functional clade member.

TABLE 3 Conserved domains of G481 (TF family: CCAAT-binding) and closely related HAP3 sequences Percent ID of conserved Species Conserved B domain SEQ from which Gene B domain Conserved to G481 ID SEQ ID NO: ID amino acid B domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved B domain B domain ′′10 Arabidopsis G481 20-109 2377 REQDRYLPIANISRIMKKALPPNGK 100 thaliana IGKDAKDTVQECVSEFISFITSEAS DKCQKEKRKTVNGDDLLWAMATLGF EDYLEPLKIYLARYR 1922 Glycine max G3470 27-116 3527 REQDRYLPIANISRIMKKALPPNGK 93 IAKDAKDTMQECVSEFISFITSEAS EKCQKEKRKTINGDDLLWAMATLGF EDYIEPLKVYLARYR 1924 Glycine max G3471 26-115 3528 REQDRYLPIANISRMIKKALPPNGK 93 IAKDAKDTMQECVSEFISFITSEAS EKCQKEKRKTINGDDLLWAMATLGF EDYIEPLKVYLARYR 2188 Glycine max G3875 25-114 3680 REQDRYLPIANISRIMKKALPANGK 91 IAKDAKETVQECVSEFISFITSEAS DKCQREKRKTINGDDLLWAMATLGF EDYIDPLKLIYLTRYR 2190 Zea mays G3876 30-119 3681 REQDRFLPIANISRIMKKAIPANGK 87 IAKDAKETVQECVSEFISFITSEAS DKCQREKRKTINGDDLLWAMATLGF EDYIEPLKVYLQKYR 1860 Oryza G3394 38-126 3483 REQDRFLPIANISRIMKKAIPANGK 87 sativa IAKDAKETVQECVSEFISFITSEAS DKCQREKRKTINGDDLLWAMATLGF EDYIEPLKVYLQKYR 1886 Zea mays G3434 18-107 3502 REQDRFLPIANISRIMKKAVPANGK 85 IAKDAKETLQECVSEFISFVTSEAS DKCQKEKRKTINGDDLLWAMATLGF EEYVEPLKIYLQKYK ′952 Arabidopsis G1364 29-118 2941 REQDRFLPIANISRIMKRGLPANGK 85 thaliana IAKDAKEIVQECVSEFISFVTSEAS DKCQREKRKTINGDDLLWAMATLGF EDYMEPLKVYLMRYR 1932 Glycine max G3475 23-112 3532 REQDRFLPIANVSRIMKKALPANAK 84 ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVEPLKGYLQRFR 394 Arabidopsis G485 20-109 2616 REQDRFLPIANVSRIMKKALPANAK 84 thaliana ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVEPLKVYLQKYR 1934 Glycine max G3476 26-115 3533 REQDRFLPIANVSRIMKKALPANAK 84 ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EEYVEPLKIYLQRFR 1476 Arabidopsis G2345 28-117 3234 REQDRFLPIANISRIMKRGLPLNGK 84 thaliana IAKDAKETMQECVSEFISFVTSEAS DKCQREKRKTINGDDLLWAMATLGF EDYIDPLKVYLMRYR 1930 Glycine max G3474 25-114 3531 REQDRFLPIANVSRIMKKALPANAK 84 ISKEAKETVQECVSEFISFITGEAS DKCQKEKRKTINGDDLLWAMTTLGF EDYVDPLKIYLHKYR 1936 Glycine max G3478 23-112 3634 REQDRFLPIANVSRIMKKALPANAK 84 ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVEPLKGYLQRFR ′′12 Arabidopsis G482 26-115 2378 REQDRFLPIANVSRIMKKALPANAK 83 thaliana ISKDAKETMQECVSEFISFVTGEAS DKCQKEKRKTINGDDLLWAMTTLGF EDYVEPLKVYLQRFR 1888 Zea mays G3435 22-111 3503 REQDRFLPIANVSRIMKKALPANAK 83 ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVEPLKHYLHKFR 1926 Glycine max G3472 25-114 3529 REQDRFLPIANVSRIMKKALPANAK 83 ISKEAKETVQECVSEFISFITGEAS DKCQKEKRKTINGDDLLWAMTTLGF EEYVEPLKVYLHKYR 1890 Zea mays G3436 20-109 3504 REQDRFLPIANVSRIMKKALPANAK 83 ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVEPLKLYLHKFR 1866 Oryza G3397 23-112 3486 REQDRFLPIANVSRIMKKALPANAK 82 sativa ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYVDPLKHYLHKFR 1862 Oryza G3395 19-108 3484 REQDRFLPIANISRIMKKAVPANGK 82 sativa IAKDAKETLQECVSEFISFVTSEAS DKCQKEKRKTINGEDLLFAMGTLGF EEYVDPLKIYLHKYR 2182 Zea mays G3866 30-126 3677 REQDRFLPIANISRIMKKAIPANGK 81 TIPANGKIAKDAKETVQECVSEFIS FITSEASDKCQREKRKTINGDDLLW AMATLGFEDYIEPLKVYLQKYR 1868 Oryza G3398 21-110 3487 REQDRFLPIANVSRIMKRALPANAK 81 sativa ISKDAKETVQECVSEFISFITGEAS DKCQREKRKTINGDDLLWAMTTLGF EDYIDPLKLYLHKFR 1864 Oryza G3396 21-110 3485 KEQDRFLPIANIGRIMRRAVPENGK 77 sativa IAKDSKESVQECVSEFISFITSEAS DKCLKEKRKTINGDDLIWSMGTLGF EDYVEPLKLYLRLYR 1880 Oryza G3429 40-124 3498 ELPMANLVRLIKKVLPGKAKIGGAA 42 sativa KGLTHDCAVEFVGFVGDEASEKAKA EHRRTVAPEDYLGSFGDLGFDRYVD PMDAYIHGYR 2184 Glycine max G3873 29-118 3678 REQDRFLPIANISRIMKKALPPNGK 86 IAKDAKETVQECVSEFISFVTSEAS DKCQREKRKTINGDDLLWAMTTLGF EEYIDPLKVYLAAYR 2186 Glycine max G3874 26-114 3679 REQDRYLPIANISRIMKKALPANGK 91 IAKDAKETVQECVSEFISFITSEAS DKCQREKRKTINGDDLLWAMATLGF EDYMDPLKIYLTRYR

TABLE 4 Conserved domains of G1248 (TF family: CCAAT-binding) and closely related HAP3 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G1248 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  878 Arabidopsis G1248 50-139 2899 KEQDRLLPIANVGRIMKNILPANA 100 thaliana KVSKEAKETMQECVSEFISFVTGE ASDKCHKEKRKTVNGDDICWAMAN LGFDDYAAQLKKYLHRYR 2152 Glycine max G3837 34-123 3662 KEQDRLLPIANVGRIMKQILPPNA 88 KISKEAKETMQECVSEFISFVTGE ASDKCHKEKRKTVNGDDICWALAT LGFDDYSEPLKRYLHKYR 2150 Oryza G3835  3-92 3661 NGQDNLLPIANVGRIMKDGLPPQA 69 sativa KISKRAKETIQECATEFISFVTGE ASERCRRERRKTVNGDDVCHAMRS LGLDHYADAMHRYLQRYR 5113 Oryza G3836 33-122 5114 KEQDRLLPIANVGRIMKQILPPNA 83 sativa KISKEAKETMQECVSEFISFVTGE ASDKCHKEKRKTVNGDDVCWAFGA LGFDDYVDPMRRYLNKYR 2234 Zea mays G3931 23-111 3704 EQDRLLPIANVGRIMKQILPPNAK 83 ISKEAKETMQECVSEFIGFVTGEA SDKCHKEKRKTVNGDDLCWAFGAL GFDDYVDPMRGYLHKYR 2340 Zea mays G4273 28-117 3757 KEQDRLLPIANVGRIMKQILPPNA 83 KISKEAKETMQECVSEFISFVTGE ASDKCHKEKRKTVNGDDVCCAFGA LGFDDYVDPMRRYLHKYR

TABLE 5 Conserved domains of G620 (TF family: CCAAT-binding) and closely related HAP3 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G620 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  494 Arabidopsis G620 28-117 2668 REQDQYMPIANVIRIMRKTLPSHA 100 thaliana KISDDAKETIQECVSEYISFVTGE ANERCQREQRKTITAEDILWAMSK LGFDNYVDPLTVFINRYR 1202 Arabidopsis G1821 57-146 3080 REQDRFMPIANVIRIMRRILPAHA 83 thaliana KISDDSKETIQECVSEYISFITGE ANERCQREQRKTITAEDVLWAMSK LGFDDYIEPLTLYLHRYR 2238 Oryza G3939 31-120 3706 REQDRLMPIANVIRIMRRVLPAHA 84 sativa KISDDAKETIQECVSEYISFITGE ANERCQREQRKTITAEDVLWAMSR LGFDDYVEPLGVYLHRYR 2154 Zea mays G3839 48-137 3663 REQDRLMPVANVSRIMRQVLPPYA 73 KISDDAKEVIQECVSEFISFVTGE ANERCHTERRKTVTSEDIVWAMSR LGFDDYVAPLGAFLQRMR 2236 Zea mays G3937 35-124 3705 REQDRLMPIANVIRIMRRVLPAHA 83 KISDDAKETIQECVSEYISFITGE ANERCQREQRKTITAEDVLWAMSR LGFDDYVEPLGAYLHRYR

TABLE 6 Conserved domains of G484 (TF family: CCAAT-binding) and closely related HAP3 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G484 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  392 Arabidopsis G484 11-99 2615 KEDASLPKATMTKIIKEMLPPDVR 100 thaliana VARDAQDLLIECCVEFINLVSSES NDVCNKEDKRTIAPEHVLKALQVL GFGEYIEEVYAAYEQHK 1612 Arabidopsis G2631 11-99 3313 KEDASLPKATMTKIIKEMLPADVR 95 thaliana VARDAQDLLIECCVEFINLISSES NEVCNKEDKRTIAPEHVLKALQVL GFGEYVEEVYAAYEQHK 2240 Oryza G3940 11-99 3707 KEDVSLPKSTMFKIIKEMLPPDVR 84 sativa VARDAQDLLVECCVEFINLLSSES NEVCSREDKKTIAPEHVLRALQDL GFREYIEEVQAAYEHHK 2342 Zea mays G4275 11-99 3758 KEDVSLPKSTMVKIIKEMLPPDVR 84 VARDAQDLLVECCVEFINLLSSES NEVCSREEKKTIAPEHVIKALSDL GFREYIEEVYAAYEQHK

TABLE 7 Conserved domains of G928 (TF family: CCAAT-binding) and closely related HAP2 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G928 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  696 Arabidopsis G928 179-238 2790 DPVFVNAKQYHAIMRRRQQRAKL 100 thaliana EAQNKLIRARKPYLHESRHVHAL KRPRGSGGRFLNTK  700 Arabidopsis G931 172-231 2792 EPVFVNAKQFHAIMRRRQQRAKL 95 thaliana EAQNKLIKARKPYLHESRHVHAL KRPRGSGGRFLNTK 2230 Oryza G3926 164-222 3702 EPIFVNAKQYNAILRRRQTRAKL 78 sativa EAQNKAVKGRKPYLHESRHHHAM KRARGSGGRFLTK 2224 Zea mays G3921 148-207 3699 EPIYVNAKQYHAILRRRQTRAKL 80 EAQNKMVKGRKPYLHESRHRHAM KRARGSGGRFLNTK 2326 Zea mays G4264 155-214 3750 EPIYVNAKQYHAILRRRQTRAKL 80 EAQNKMVKNRKPYLHESRHRHAM KRARGSGGRFLNTK 2328 Zea mays G4265 149-208 3751 EPIYVNAKQYHAILRRRQTRAKL 76 EAQNKMVKGRKPYLHESRHRHAM KRARGSGGRFPHTK 2334 Zea mays G4269 103-162 3754 EPIYVNPKQYHGILRRRQLRAKL 81 EAQNKLVRARKPYLHESRHLHAM KRARGSGGRFLNTK

TABLE 8 Conserved domains of G2632 (TF family: CCAAT-binding) and closely related HAP2 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G2632 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 1614 Arabidopsis G2632 166-223 3314 EPVFVNAKQYQAILRRRQARAKA 100 thaliana ELEKKLIKSRKPYLHESRHQHAM RRPRGTGGRFAK  692 Arabidopsis G926 172-229 2788 EPVYVNAKQYEGILRRRKARAKA 79 thaliana ELERKVIRDRKPYLHESRHKHAM RRARASGGRFAK 2226 Oryza G3924 163-222 3700 EPVYVNAKQYHGILRRRQSRAKA 87 sativa ELEKKVVKSRKPYLHESRHQHAM RRARGTGGRFLNTK 2320 Zea mays G4261 175-234 3747 EPVYVNAKQYHGILRRRQSRAKA 83 ELEKKVVKARKPYLHESRHQHAM RRARGNGGRFLNTK

TABLE 9 Conserved domains of G1782 (TF family: CCAAT-binding) and closely related HAP2 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G1782 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 1162 Arabidopsis G1782 178-237 3060 EPIFVNAKQYHAILRRRKHRAKLE 100 thaliana AQNKLIKCRKPYLHESRHLHALKR ARGSGGRFLNTK  950 Arabidopsis G1363 171-230 2940 EPIFVNAKQYQAILRRRERRAKLE 91 thaliana AQNKLIKVRKPYLHESRHLHALKR VRGSGGRFLNTK 2222 Glycine max G3920 149-208 3698 EPVYVNAKQYHGILRRRQSRAKAE 76 IEKKVIKNRKPYLHESRHLHAMRR ARGNGGRFLNTK 2228 Oryza G3925 138-197 3701 EPIYVNAKQYHAILRRRQLRAKLE 85 sativa AENKLVKNRKPYLHESRHQHAMKR ARGTGGRFLNTK 5116 Zea mays G3922 171-230 5117 EPIYVNAKQYHAILRRRQTRAKLE 86 AQNKMVKNRKPYLHESRHRHAMKR ARGSGGRFLNTK 2322 Zea mays G4262 142-201 3748 EPIYVNAKQYHAILRRRQLRAKLE 86 AENKLVKSRKPYLHESRHLHAMKR ARGTGGRFLNTK 2324 Zea mays G4263 137-196 3749 EPIYVNAKQYHAILRRRQLRAKLE 86 AENKLVKSRKPYLHESRHLHAMKR ARGTGGRFLNTK 2336 Zea mays G4270 131-191 3755 EAPIYVNAKQYDAIMRRRCARAKA 76 ERENRLVKGRKPYLHESRHQHALR RPRGSGGRFLNTK

TABLE 10 Conserved domains of G1334 (TF family: CCAAT-binding) and closely related HAP2 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G1334 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 936 Arabidopsis G1334 133-190 2930 DGTIYVNSKQYHGIIRRRQSRAKA 100 thaliana EKLSRCRKPYMHHSRHLHAMRRPR GSGGRFLNTK 694 Arabidopsis G927 136-199 2789 STIYVNSKQYHGIIRRRQSRAKAA 82 thaliana AVLDQKKLSSRCRKPYMHHSRHLH ALRRPRGSGGRFLNTK

TABLE 11 Conserved domains of G929 (TF family: CCAAT-binding) and closely related HAP2 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G929 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  698 Arabidopsis G929  98-157 2791 EPVFVNAKQYHGILRRRQSRAKL 100 thaliana EARNRAIKAKKPYMHESRHLHAI RRPRGCGGRFLNAK 1474 Arabidopsis G2344 100-159 3233 EPVFVNAKQYHGILRRRQSRARL 86 thaliana ESQNKVIKSRKPYLHESRHLHAI RRPRGCGGRFLNAK 2330 Zea mays G4267 110-169 3752 EPVYVNAKQYNAILRRRQSRAKA 68 ESERKLVKGRKPYLHESRHQHAL KRARGAGGRTLNSK 2332 Zea mays G4268 110-169 3753 EPVYVNAKQYNAILRRRQSRAKA 70 ESERKLIKGRKPYLHESRHQHAL KRARGAGGRFLNSK

TABLE 12 Conserved domains of G3546 (TF family: CCAAT-binding) and closely related HAP5 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G3546 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 2012 Oryza G3546 78-175 3572 REIEHTTDFKNHNLPLARIKKIMK 100 sativa ADEDVRMIAAEAPVVFARACEMFI LELTHRGWAHAEENKRRTLQKSDI AAAIARTEVFDFLVDIVPRDEAKD AE 5119 Sorghum G3910 73-170 5120 REIEATTDFKNHNLPLARIKKIMK 95 bicolor ADEDVRMIAAEAPVVFARACEMFI LELTHRGWAHAEENKRRTLQKSDI AAAVARTEVFDFLVDIVPRDEAKE AD 2218 Zea mays G3911 70-167 3695 REIEATTDFKNHNLPLARIKKIMK 95 ADEDVRMIAAEAPVVFARACEMFI LELTHRGWAHAEENKRRTLQKSDI AAAIARTEVFDFLVDIVPRDDGKD AD 2216 Zea mays G3909 73-170 3694 REIEATTDFKNHNLPLARIKKIMK 95 ADEDVRMIAAEAPVVFSRACEMFI LELTHRGWAHAEENKRRTLQKSDI AAAVARTEVFDFLVDIVPRDEAKD AD 2316 Zea mays G4258 70-167 3744 REIEATTDFKNHNLPLARIKKIMK 95 ADEDVRMIAAEAPVVFARACEMFI LELTHRGWAHAEENKRRTLQKSDI AAAVARTEVFDFLVDIVPRDEARD AD

TABLE 13 Conserved domains of G489 (TF family: CCAAT-binding) and closely related HAP5 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G3546 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  398 Arabidopsis G489 68-164 2618 KEIEKTTDFKKHSLPLARIKKIM 100 thaliana KADEDVRMISAEAPVVFARACEM FILELTLRSWNHTEENKRRTLQK NDIAAAVTRTDIFDFLVDIVPRE DLRDE  554 Arabidopsis G714 58-150 2705 KEIEKTTDFKNHSLPLARIKKIM 98 thaliana KADEDVRMISAEAPVVFARACEM FILELTLRSWNHTEENKRRTLQK NDIAAAVTRTDIFDFLVDIVPRE D 2016 Glycine max G3547 89-185 3573 QEIEKVTDFKNHSLPLARIKKIM 93 KADEDVRMISAEAPVIFARACEM FILELTLRSWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2020 Glycine max G3549 93-189 3576 QEIEQTIDFKNHSLPLARIKKIM 83 KADEDVRMISAEAPVIFAKACEM FILELTLRSWIHTEENKRRTLQK NDIAAAISRNDVFDFLVDIIPRD ELKEE 2022 Glycine max G3550 94-190 3577 QEIEQTIDFKNHSLPLARIKKIM 83 KADEDVRMISAEAPVIFAKACEM FILELTLRSWIHTEENKRRTLQK NDIAAAISRNDVFDFLVDIIPRD ELKEE 2208 Medicago G3896 89-185 3690 QEIEKVTDFKNHSLPLARIKKIM 93 truncatula KADEDVRMISAEAPVIFARACEM FILELTLRSWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2004 Oryza G3542 93-189 3568 EEIEQTTDFKNHSLPLARIKKIM 86 sativa KADEDVRMISAEAPVVFAKACEV FILELTLRSWMHTEENKRRTLQK NDIAAAITRTDIYDFLVDIVPRD EMKEE 2008 Oryza G3544 89-185 3570 VDIEQTTDFKNHSLPLARIKKIM 86 sativa KADEDVRMISAEAPVIFAKACEI FILELTLRSWMHTEENKRRTLQK NDIAAAITRTDMYDFLVDIVPRD DLKEE 2010 Oryza G3545 89-189 3571 EVEQMTEFKLPNLPLARIKKIMK 64 sativa ADEDVKMIAGEAPALFAKACEMF ILDMTLRSWQHTEEGRRRTLQRS DVEAVIKKTDIFDFLVDIITDDK MKDDGMGSQ 5122 Physcomitrella G3867 53-149 5123 QEMEQVNDFKTHQLPLARIKKIM 77 patens KSDEDVKMIAAEAPVLFSKACEM FILELTLRSWIHTEENKRRTLQR NDIAGAITRGDIFDFLVDIVPRD ELKEE 2028 Solanum G3553 62-158 3580 QEIEHVTDFKNHSLPLARIKKIM 92 lycopersicum KADEDVRMISAEAPVVFARACEM FILELTLRAWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2030 Solanum G3554 90-186 3581 QEIEHVTDFKNHSLPLARIKKIM 92 lycopersicum KADEDVRMISAEAPVVFARACEM FILELTLRAWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2032 Solanum G3555 54-150 3582 QEIEQVNDFKNHQLPLARIKKIM 82 lycopersicum KADEDVRMISAEAPVLFAKACEL FILELTIRSWLHAEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRD EIKDE 2206 Solanum G3894 90-186 3689 QEIEHVTDFKNHSLPLARIKKIM 92 lycopersicum KADEDVRMLSAEAPVVFARACEM FILELTLRAWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2202 Solanum G3892 62-158 3687 QEIEHVTDFKNHSLPLARIKKIM 92 tuberosum KADEDVRMISAEAPVVFARACEM FILELTLRAWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2204 Solanum G3893 88-184 3688 QEIEHVTDFKNHSLPLARIKKIM 92 tuberosum KADEDVRMISAEAPVVFARACEM FILELTLRAWNHTEENKRRTLQK NDIAAAITRTDIFDFLVDIVPRE DLKDE 2024 Zea mays G3551 87-187 3578 TEIEATTDFKNHNLPLARIKKIM 84 KADEDVRMISAEAPVVFAKACEI FILELTLRSWMHTEVNKRRTLQK NDIAAAITRTDIYDFLVDIVPRD EMKEDGIGL 2026 Zea mays G3552 87-183 3579 TEIEATTDFKNHNLPLARIKKIM 85 KADEDVRMISAEAPVVFAKACEI FILELTLRSWMHTEENKRRTLQK NDIAAAITRTDIYDFLVDIVPRD EMKED 2310 Zea mays G4256 84-180 3742 DEIKQANDFKIHTLPLARIKKIM 82 KADEDVRMISAEAPVVFAKACEV FILELTLRSWMHTEENKRRTLQK NDIAAAITRTDIYDFLVDIIPRD EMKEE 2313 Zea mays G4257 90-186 3743 TEIEATADFRNHNLPLARIKKIM 84 KADEDVRMISAEAPVVFAKACEI FILELTLRSWMHTEENKRRTLQK NDIAAAITRTDIYDFLVDIVPRD EMKDD

TABLE 14 Conserved domains of G1836 (TF family: CCAAT-binding) and closely related HAP5 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G1836 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 1212 Arabidopsis G1836 24-110 3077 KEMEGNLDFKNHDLPITRIKKIM 100 thaliana KYDPDVTMIASEAPILLSKACEM FIMDLTMRSWLHAQESKRVTLQK SNVDAAVAQTVIFDFLLD 1196 Arabidopsis G1818 24-116 3087 KGMEGDLNVKNHEFPISRIKRIM 75 thaliana KFDPDVSMIAAEAPNLLSKACEM FVMDLTMRSWLHAQESNRLTIRK SDVDAVVSQTVIFDFLRDDVPKD E

TABLE 15 Conserved domains of G483 (TF family: CCAAT-binding) and closely related HAP5 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G483 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain  390 Arabidopsis G483 64-160 QEIEHTTDFKNHTLPLARIKKIM 100 thaliana KADEDVRMISAEAPVIFAKACEM FILELTLRAWIHTEENKRRTLQK NDIAAAISRTDVFDFLVDIIPRD ELKEE 2210 Daucus G3899 89-185 QEIGQTPDFKNHSLPLARIKKIM 89 carota KADEDVRMISSEAPVIFAKACEM FILELTMRSWLLTEENKRRTLQK NDIAAAISRTDIFDFLVDIIPRD ELKEE 2212 Daucus G3900 70-166 QEIEQTTDFKNHSLPLARIKKIMK 86 carota ADEDVRMISSEAPVVFAKACEMFI MDLTMRSWSHTEENKRRTLQKNDI AAAVSRTDVFDFLVDIIPKDEMKE D 2018 Glycine max G3548 77-173 QEIEQTIDFKNHSLPLARIKKIMK 94 ADEDVRMISAEAPVIFAKACEMFI LELTLRSWIHTEENKRRTLQKNDI AAAISRNDVFDFLVDIIPRDELKE E 2214 Gossypium G3907 92-184 HEIEQTTDFKNHSLPLARIKKIMK 93 arboreum ADEDVRMISAEAPVIFAKACEMFV LELTLRSWIHTEENKRRTLQKNDI AAAISRTDVFDFLVDIIPGTE

TABLE 16 Conserved domains of G3074 (TF family: CCAAT-binding) and closely related HAP-like sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G3074 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 1826 Arabidopsis G3074  3-86 3460 KKLDTRFPAARIKKIMQADEDV 100 thaliana GKIALAVPVLVSKSLELFLQDL CDRTYEITLERGAKTVSSLHLK HCVERYNVFDFLREVVSK 2304 Zea mays G4253 10-86 3739 PAARIKKIMQADEDVGKIALAV 84 PVLVSRSLELFLQDLIDRTYEI TLQSGAKTLNSFHLKQCVKRYS SFDFLTEVVSK 2306 Zea mays G4254 10-86 3740 PAARIKKIMQADEDVGKIALAV 83 PVLVSRALELFLQDLIDRTYEI TLQSGAKTLNSFHLKQCVKRYS SFDFLTEVVSK 2308 Zea mays G4255 10-86 3741 PAPRIKKIMQTDEDVGKIAQAV 79 PVLVSKALELFLQDLCDRTYDI TIRKGVKTVGSSHLKQCIQTYN VYDFLREVVSK

TABLE 17 Conserved domains of G1646 (TF family: CCAAT-binding) and closely related HAP5 sequences Percent ID of conserved Species Conserved domain SEQ from which Gene domain Conserved to G1646 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved domain domain 1100 Arabidopsis G1646 66-162 3026 QEIEQVNDFKNHQLPLARIKKI 100 thaliana MKADEDVRMISAEAPILFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPREEIKEE  556 Arabidopsis G715 53-149 2706 QEIEQVNDFKNHQLPLARIKKI 97 thaliana MKADEDVRMISAEAPILFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPRDEIKDE 2198 Glycine max G3886 59-155 3685 QEIEHVNDFKNHQLPLARIKKI 95 MKADEDVRMISAEAPILFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPRDEIKDD 2192 Gossypium G3883 54-150 3682 QEIEQVNDFKNHQLPLARIKKI 97 raimondii MKADEDVRMISAEAPILFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPRDEIKDE 2194 Nicotiana G3884 47-143 3683 QEIEQVNDFKNHQLPLARIKKI 98 benthamiama MKADEDVRMISAEAPILFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPRDEIKEE 2006 Oryza G3543 55-153 3569 QEAERASASDFKNHQLPLARIK 87 sativa KIMKADEDVRMISAEAPVLFAK ACELFILELTIRSWLHAEENKR RTLQRNDVAAAIARTDVFDFLV DIVPREEAKEE 2196 Solanum G3885 54-150 3684 QEIEQVNDFKNHQLPLARIKKI 96 tuberosum MKADEDVRMISAEAPVLFAKAC ELFILELTIRSWLHAEENKRRT LQKNDIAAAITRTDIFDFLVDI VPRDEIKDE 2200 Zea mays G3889 54-152 3686 QEAERASASDFKNHQLPLARIK 87 KIMKADEDVRMISAEAPVLFAK ACELFILELTIRSWLHAEENKR RTLQRNDVAAAIARTDVFDFLV DIVPREEAKEE 2317 Zea mays G4259 55-153 3746 QEAERASASDFKNHQLPLARIK 87 KIMKADEDVRMISAEAPVLFAK ACELFILELTIRSWLHAEENKR RTLQRNDVAAAIARTDVFDFLV DIVPREEAKEE

TABLE 18 Conserved domains of G682 (TF family: MYB-related) and closely related MYB sequences Percent ID of conserved Species Conserved MYB domain SEQ from which Gene MYB domain Conserved to G682 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved MYB domain MYB domain  550 Arabidopsis G682 33-77 2703 VNMSQEEEDLVSRMHKLVG 100 thaliana DRWELIAGRIPGRTAGEIE RFWVMKN  196 Arabidopsis G226 38-82 2477 ISMTEQEEDLISRMYRLVG 62 thaliana NRWDLIAGRVVGRKANEIE RYWIMRN 1654 Arabidopsis G2718 32-76 3340 IAMAQEEEDLICRMYKLVG 80 thaliana ERWDLIAGRIPGRTAEEIE RFWVMKN  194 Arabidopsis G225 36-80 2476 VKMSEEEEDLISRMYKLVG 80 thaliana DRWELIAGRIPGRTPEEIE RYWLMKH 1858 Oryza G3393 31-75 3482 VHFTEEEEDLVFRMHRLVG 71 sativa NRWELIAGRIPGRTAKEVE MFWAVKH 1882 Zea mays G3431 31-75 3499 VDFTEAEEDLVSRMHRLVG 70 NRWEIIAGRIPGRTAEEVE MFWSKKY 1894 Zea mays G3444 31-75 3506 VDFTEAEEDLVSRMHRLVG 70 NRWEIIAGRIPGRTAEEVE MFWSKKY   14 Oryza G3392 32-76 2379 VHFTEEEEDIVFRMHRLVG 68 sativa NRWELIAGRIPGRTAEEVE KFWAIKH 1906 Glycine max G3450 20-64 3512 IHMSEQEEDLIRRMYKLVG 68 GDKWNLIAGRIPGRKAEEI ERFWIMRH 1194 Arabidopsis G1816 30-74 3076 INMTEQEEDLIFRMYRLVG 64 thaliana DRWDLIAGRVPGRQPEEIE RYWIMRN 1904 Glycine max G3449 26-70 3511 VEFSEDEETLIIRMYKLVG 63 ERWSLIAGRIPGRTAEEIE KYWTSRF 2232 Arabidopsis G3930 33-77 3703 INMTEQEEDLIFRMYRLVG 62 thaliana DRWDLIARRVVGREAKEIE RYWIMRN 1902 Glycine max G3448 26-70 3510 VEFSEDEETLIIRMYKLVG 61 ERWSIIAGRIPGRTAEEIE KYWTSRF 1898 Glycine max G3446 26-70 3508 VEFSEAEEILIAMVYNLVG 56 ERWSLIAGRIPGRTAEEIE KYWTSRF 1896 Glycine max G3445 25-69 3507 VEFSEAEEILIAMVYNLVG 56 ERWSLIAGRIPGRTAEEIE KYWTSRF 1900 Glycine max G3447 26-70 3509 VEFSEAEEILIAMVYNLVG 56 ERWSLIAGRIPGRTAEEIE KYWTSRF

TABLE 19 Conserved domains of G207 (TF family: MYB-(R1)R2R3) and closely related MYB sequences Percent ID of conserved Species Conserved MYB domain SEQ from which Gene MYB domain Conserved to G207 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved MYB domain MYB domain  178 Arabidopsis G207  6-106 2468 KGPWSQEEDEQLRRMVEKYGP 100 thaliana RNWSAISKSIPGRSGKSCRLR WCNQLSPEVEHRPFSPEEDET IVTARAQFGNKWATIARLLNG RTDNAVKNHWNSTLKRK  214 Arabidopsis G242  6-106 2486 KGPWSPEEDEQLRRLVVKYGP 90 thaliana RNWTVISKSIPGRSGKSCRLR WCNQLSPQVEHRPFSAEEDET IARAHAQFGNKWATIARLLNG RTDNAVKNHWNSTLKRK  198 Arabidopsis G227 13-113 2478 KGPWSPEEDDLLQRLVQKHGP 84 thaliana RNWSLISKSIPGRSGKSCRLR WCNQLSPEVEHRAFSQEEDET IIRAHARFGNKWATISRLLNG RTDNAIKNHWNSTLKRK 2268 Glycine max G4220 15-115 3721 KGPWSPEEDEALRALVQAHGP 84 RNWSVISKSIPGRSGKSCRLR WCNQLSPQVAHRPFSQEEDEA IIMAHAKFGNKWATIARLLNG RTDNAVKNHWNSTLKRK 2270 Glycine max G4221  5-106 3722 KGPWSPEEDEALRRLVQAHGP 84 RNWSVISKSVPGRSGKSCRLR WCNQLSPQVAHRPFSPDEDEA IVRAHARFGNKWATIARLLNN GRTDNAVKNHWNSTLKRK 2276 Glycine max G4224  5-105 3725 KGPWSPEEDEALRRLVQTYGP 84 RNWSVISKSIPGRSGKSCRLR WCNQLSPEVERRPFTAEEDEA ILKAHARFGNKWATIARFLNG RTDNAIKNHWNSTLKRK  202 Arabidopsis G230 13-113 2480 KGPWSPEEDDLLQSLVQKHGP 82 thaliana RNWSLISKSIPGRSGKSCRLR WCNQLSPEVEHRGFTAEEDDT IILAHARFGNKWATIARLLNG RTDNALKNHWNSTLKRK 2272 Glycine max G4222  5-105 3723 KGPWSPEEDEALQKLVEKHGP 82 RNWSLISKSIPGRSGKSCRLR WCNQLSPQVEHRAFTAEEDDT IIRAHARFGNKWATIARLLHG RTDNAIKNHWNSTLKRK 2274 Glycine max G4223 11-111 3724 KGPWSPEEDEALQKLVEKHGP 82 RNWSLISKSIPGRSGKSCRLR WCNQLSPQVEHRAFTHEEDDT IIRAHARFGNKWATIARLLHG RTDNAIKNHWNSTLKRK 2294 Oryza G4234 17-117 3734 KGPWSPEEDEALQRLVGRHGA 82 sativa RNWSLISKSIPGRSGKSCRLR WCNQLSPQVEHRPFTPEEDDT ILRAHARFGNKWATIARLLAG RTDNAIKNHWNSTLKRK 2284 Zea mays G4228 21-121 3729 KGPWSPEEDEALQRLVARHGA 80 RNWSLISRSIPGRSGKSCRLR WCNQLSPQVEHRPFTAEEDDT ILRAHARFGNKWATIARLLSG RTDNAIKNHWNSTLKRK 2264 Glycine max G4218 31-131 3719 KGPWSAEEDRILTGLVERYGP 78 RNWSLISRYIKGRSGKSCRLR WCNQLSPAVEHRPFSAQEDDT IIAAHAQYGNRWATIARLLPG RTDNAVKNHWNSTLKRR 2266 Glycine max G4219 31-131 3720 KGPWSAQEDRILTRLVEQYGP 78 RNWSLISRYIKGRSGKSCRLR WCNQLSPTVEHRPFSTQEDET IIAAHARYGNRWATIARLLPG RTDNAVKNHWNSTLKRR 2286 Zea mays G4229 21-121 3730 KGPWSPEEDEALQRLVRRHGA 77 RNWSLISRSVPGRSGKSCRLR WCNQLSPRVEHRPFTPDEDDA ILRAHARFGNKWATIARLLSG RTDNAIKNHWNSTLKRE 2280 Zea mays G4226 11-111 3727 KGPWSPEEDEALRRLVERHGA 76 RNWTAIGRGIPGRSGKSCRLR WCNQLSPQVERRPFTPEEDAA ILAAHARLGNRWAAIARLLPG RTDNAVKNHWNSSLKRK 2302 Oryza G4238 11-111 3738 KGPWSPEEDEALRRLVERHGA 75 sativa RNWTAIGRGIPGRSGKSCRLR WCNQLSPQVERRPFTAEEDAA ILRAHARLGNRWAAIARLLPG RTDNAVKNHWNSSLKRK 2296 Oryza G4235 15-115 3735 RGPWSPEEDEALRRLVERHGA 74 sativa RNWTAIGREIPGRSGKSCRLR WCNQLSPQVERRPFTAEEDAT ILRAHARLGNRWAAIARLLQG RTDNAVKNHWNCSLKRK 2298 Oryza G4236 20-120 3736 KGSWSPEEDEQLRGAVARHGP 74 sativa RNWTAISEEVPGRSGKSCRLR WCNQLSPGVHRRPFTPDEDAL IVAAHAKYGNKWATIARLLDG RTDNSVKNHWNSSLRRN 2278 Glycine max G4225 39-139 3726 KGPWSAKEDRILTGLVEAHGP 74 RNWASISRHIKGRSGKSCRLR WCNQLSPTVEHRPFSTREDEV ILHAHARFGNKWATIARMLPG RTDNAVKNHWNATLKRR 2288 Zea mays G4230 11-111 3731 RGPWSPEEDDALRRLVERHGA 73 RNWTAIGREIPGRSGKSCRLR WCNQLSPQVERRPFTAEEDAA IVRAHARLGNRWAAIARLLPG RTDNAVKNHWNCSLKRK 2282 Zea mays G4227 20-120 3728 KGSWSPEEDALLTRLVEQHGP 71 HRWSLISAPIPGRSGKSCRLR WCNQLSPDVHHRPFTPHEDAL ILAAHARYGNKWATIARLLPG RTDNSIKNHWNSNLRRC 2292 Zea mays G4232 11-103 3733 RGPWSPEEDDALRRLVERHGA 70 RNWTAIGREIPGRSGKSCPLR WCNQLSPQVERPPFTPEEDAA ILAAHARLGNRWAAIARLLPG RTDNAVKNH 2300 Oryza G4237 10-110 3737 KGSWRAEEDALLTRLVAQHGP 69 sativa HRWSIISGAIPGRSGKSCRLR WCNQLSPAVQHRPFTPQEDAL LAAAHARHGNKWATIARLLPG RTDNSVKNHWNSNLRRC 2290 Zea mays G4231 12-112 3732 RGPWSPEEDEALRRLVERHGA 67 RNWTAIGRGVPGRSGKSCRLR WCNQLGRGGARRPFTADEDAA IARAHARLGNRWAAIARLLPG RTDNAVKNHWNCSLKRK

TABLE 20 Conserved domains of G867 (TF family: AP2) and closely related AP2 sequences Percent ID of conserved AP2 or B3 Conserved domain Species AP2 or B3 to G867 SEQ from which Gene domain Conserved AP2 or B3 ID SEQ ID NO: ID amino acid domain domain, NO: is derived (GID) coordinates SEQ ID NO: AP2 or B3 conserved domains respectively   16 Arabidopsis G867 AP2: 2380 AP2 domain sequence: 100 thaliana  59-124 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEDEAARAYDVA VHRFRRRDAVTNFKDVKMDEDE B3: 2381 B3 domain sequence: 100 AP2: 184-276 AEALFEKAVTPSDVGKLNRLVI PKHHAEKHFPLPSSNVSVKGVL LNFEDVNGKVWRFRYSYWNSSQ SYVLTKGWSRFVKEKNLRAGDV VSFSR  746 Arabidopsis G993 AP2: 2824 AP2 domain sequence: 89 thaliana  69-134 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEEEAASSYDIA VRRFRGRDAVTNFKSQVDGNDA B3: 2825 B3 domain sequence: 77 191-290 REVLFEKTVTPSDVGKLNRLVI PKQHAEKHFPLPAMTTAMGNPS PTKGVLINLEDRTGKVWRFRYS YWNSSQSYVLTKGWSRFVKEKN LRAGDVVCFER 1276 Arabidopsis G1930 AP2: 3123 AP2 domain sequence: 86 thaliana  59-124 SSRFKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEDEAARAYDVA AHRFRGRDAVTNFKDTTFEEEV B3: 3124 B3 domain sequence: 86 179-273 AELLFEKTVTPSDVGKLNRLVI PKHQAEKHFPLPLGNNNVSVKG MLLNFEDVNGKVWRFRYSYWNS SQSYVLTKGWSRFVKEKRLCAG DLISFKR 1856 Oryza G3391 AP2: 3480 AP2 domain sequence: 84 sativa  79-148 SSKFKGVVPQPNGRWGAQIYER HQRVWLGTFAGEDDAARAYDVA AQRFRGRDAVTNFRPLAEADPD AAAE B3: 3481 B3 domain sequence: 83 215-300 LFDKTVTPSDVGKLNRLVIPKQ HAEKHFPLQLPSAGGESKGVLL NFEDAAGKVWRFRYSYWNSSQS YVLTKGWSRFVKEKGLHADG 1914 Glycine max G3455 AP2: 3519 AP2 domain sequence: 83  74-143 SSKYKGVVPQPNGRWGSQIYEK HQRVWLGTFNEEDEAARAYDVA VQRFRGKDAVTNFKPLSGTDDD DGES B3: 3520 B3 domain sequence: 79 201-300 REQLFQKAVTPSDVGKLNRLVI PKQHAEKHFPLQSAANGVSATA TAAKGVLLNFEDVGGKVWRFRY SYWNSSQSYVLTKGWSRFVKEK NLKAGDTVCFQR 1910 Glycine max G3452 AP2: 3515 AP2 domain sequence: 83  51-116 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEDEAARAYDIA ALRFRGPDAVTNFKPPAASDDA B3: 3516 B3 domain sequence: 78 171-266 LFEKTVTPSDVGKLNRLVIPKQ HAEKHFPLSGSGDESSPCVAGA SAAKGMLLNFEDVGGKVWRFRY SYWNSSQSYVLTKGWSRFVKEK NLRAGDAV 1912 Glycine max G3453 AP2: 3517 AP2 domain sequence: 83  57-122 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEDEAVRAYDIV AHRFRGRDAVTNFKPLAGADDA B3: 3518 B3 domain sequence: 77 177-272 LVEKTVTPSDVGKLNRLVIPKQ HAEKHFPLSGSGGGALPCMAAA AGAKGMLLNFEDVGGKVWRFRY SYWNSSQSYVLTKGWSRFVKEK NLRAGDAV 1884 Zea mays G3432 AP2: 3500 AP2 domain sequence: 82  75-140 SSRYKGVVPQPNGRWGAQIYER HQRVWLGTFAGEADAARAYDVA AQRFRGRDAVTNFRPLADADPD B3: 3501 B3 domain sequence: 82 212-299 LFDKTVTPSDVGKLNRLVIPKQ HAEKHFPLQLPSAGGESKGVLL NLEDAAGKVWRFRYSYWNSSQS YVLTKGWSRFVKEKGLQAGDVV 1854 Oryza G3389 AP2: 3478 AP2 domain sequence: 82 sativa  64-129 SSRYKGVVPQPNGRWGAQIYER HARVWLGTFPDEEAAARAYDVA ALRFRGRDAVTNRAPAAEGAS B3: 3479 B3 domain sequence: 78 171-266 LFEKAVTPSDVGKLNRLVVPKQ QAERHFPFPLRRHSSDAAGKGV LLNFEDGDGKVWRFRYSYWNSS QSYVLTKGWSRFVREKGLRPGD TV   44 Arabidopsis G9 AP2: 2398 AP2 domain sequence: 81 thaliana  62-127 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEQEEAARSYDIA ACRFRGRDAVVNFKNVLEDGDL B3: 2399 B3 domain sequence: 89 184-277 REVLFEKAVTPSDVGKLNRLVI PKQHAEKHFPLPSPSPAVTKGV LINFEDVNGKVWRFRYSYWNSS QSYVLTKGWSRFVKEKNLRAGD VVTFER 1908 Glycine max G3451 AP2: 3513 AP2 domain sequence: 91  80-141 SSKYKGVVPQPNGRWGAQIYEK HQRVWLGTFNEEDEAARAYDIA AQRFRGKDAVTNFKPLAG B3: 3514 B3 domain sequence: 78 209-308 LFEKAVTPSDVGKLNRLVIPKQ HAEKHFPLQSSNGVSATTIAAV TATPTAAKGVLLNFEDVGGKVW RFRYSYWNSSQSYVLTKGWSRF VKEKNLKAGDTV 1852 Oryza G3388 AP2: 3476 AP2 domain sequence: 78 sativa  66-129 SSRYKGVVPQPNGRWGAQIYER HARVWLGTFPDEEAAARAYDVA ALRYRGRDAATNFPGAAASA B3: 3477 B3 domain sequence: 76 181-274 LFEKAVTPSDVGKLNRLVVPKQ HAEKHFPLRRAASSDSASAAAT GKGVLLNFEDGEGKVWRFRYSY WNSSQSYVLTKGWSRFVREKGL RAGDTI 5125 Oryza G3390 AP2: 5126 AP2 domain sequence: 77 sativa  66-131 SSKYKGVVPQPNGRWGAQIYER HQRVWLGTFTGEAEAARAYDVA AQRFRGRDAVTNFRPLAESDPE B3: 5127 B3 domain sequence: 70 192-294 LFDKTVTPSDVGKLNRLVIPKQ HAEKHFPLQLPPPTTTSSVAAA ADAAAGGGDCKGVLLNFEDAAG KVWKFRYSYWNSSQSYVLTKGW SRFVKEKGLHAGDAV

TABLE 21 Conserved domains of G28 (TF family: AP2) and closely related AP2 sequences Percent ID of conserved Species Conserved AP2 domain SEQ from which Gene AP2 domain Conserved to G28 ID SEQ ID NO: ID amino acid AP2 domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved AP2 domain AP2 domain    2 Arabidopsis G28 144-208 2373 KGKHYRGVRQRPWGKFAAEIRD 100 thaliana PAKNGARVWLGTFETAEDAALA YDRAAFRMRGSRALLNFPLRV 2054 Brassica G3659 130-194 3594 KGKHYRGVRQRPWGKFAAEIRD 100 oleracea PAKNGARVWLGTFETAEDAALA YDRAAFRMRGSRALLNFPLRV  752 Arabidopsis G1006 113-177 2828 KAKHYRGVRQRPWGKFAAEIRD 98 thaliana PAKNGARVWLGTFETAEDAALA YDLAAFRMRGSRALLNFPLRV 2076 Glycine max G3717 130-194 3613 KGKHYRGVRQRPWGKFAAEIRD 98 PAKNGARVWLGTFETAEDAALA YDRAAYRMRGSRALLNFPLRV 2078 Glycine max G3718 139-203 3614 KGKHYRGVRQRPWGKFAAEIRD 96 PAKNGARVWLGTFETAEDAALA YDRAAYRMRGSRALLNFPLRI 2056 Brassica G3660 119-183 3595 KGKHYRGVRQRPWGKFAAEIRD 96 oleracea PAKKGAREWLGTFETAEDAALA YDRAAFRMRGSRALLNFPLRV 2168 Oryza G3848 149-213 3670 RGKHYRGVRQRPWGKFAAEIRD 95 sativa PAKNGARVWLGTFDTAEDAALA YDRAAYRMRGSRALLNFPLRI 2058 Zea mays G3661 126-190 3596 RGKHYRGVRQRPWGKFAAEIRD 92 PARNGARVWLGTYDTAEDAALA YDRAAYRMRGSRALLNFPLRI 2178 Triticum G3864 127-191 3675 RGKHFRGVRQRPWGKFAAEIRD 90 aestivum PAKNGARVWLGTFDSAEDAAVA YDRAAYRMRGSRALLNFPLRI 2172 Zea mays G3856 140-204 3672 RGKHYRGVRQRPWGKFAAEIRD 90 PAKNGARVWLGTYDSAEDAAVA YDRAAYRMRGSRALLNFPLRI    4 Oryza G3430 145-209 2374 RGKHYRGVRQRPWGKFAAEIRD 90 sativa PAKNGARVWLGTFDSAEEAAVA YDRAAYRMRGSRALLNFPLRI 2158 Solanum G3841 102-166 3665 KGRHYRGVRQRPWGKFAAEIRD 84 lycopersicum PAKNGARVWLGTYETAEEAAIA YDKAAYRMRGSKAHLNFPHRI   56 Arabidopsis G22  88-152 2406 KGMQYRGVRRRPWGKFAAEIRD 81 thaliana PKKNGARVWLGTYETPEDAAVA YDRAAFQLRGSKAKLNFPHLI

TABLE 22 Conserved domains of G47 (TF family: AP2) and closely related AP2 sequences Percent ID of conserved Species Conserved AP2 domain SEQ from which Gene AP2 domain Conserved to G47 ID SEQ ID NO: ID amino acid AP2 domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved AP2 domain AP2 domain    6 Arabidopsis G47 10-75 2375 SQSKYKGIRRRKWGKWVSEIRV 100 thaliana PGTRDRLWLGSFSTAEGAAVAH DVAFFCLHQPDSLESLNFPHLL    8 Arabidopsis G2133 10-77 2376 DQSKYKGIRRRKWGKWVSEIRV 89 thaliana PGTRQRLWLGSFSTAEGAAVAH DVAFYCLHRPSSLDDESFNFPH LL 2046 Oryza G3649 15-87 3590 EMMRYRGVRRRRWGKWVSEIRV 72 sativa PGTRERLWLGSYATAEAAAVAH DAAVCLLRLGGGRRAAAGGGGG LNFPARA 2038 Oryza G3644 52-122 3586 ERCRYRGVRRRRWGKWVSEIRV 72 sativa PGTRERLWLGSYATPEAAAVAH DTAVYFLRGGAGDGGGGGATLN FPERA 2036 Glycine max G3643 13-78 3585 TNNKLKGVRRRKWGKWVSEIRV 68 PGTQERLWLGTYATPEAAAVAH DVAVYCLSRPSSLDKLNFPETL 2048 Zea mays G3650 75-139 3591 RRCRYRGVRRRAWGKWVSEIRV 65 PGTRERLWLGSYAAPEAAAVAH DAAACLLRGCAGRRLNFPGRA

TABLE 23 Conserved domains of G1792 (TF family: AP2) and closely related AP2 sequences Percent ID of conserved AP2 or EDLL domain Conserved to G1792 Species AP2 and Conserved conserved SEQ from which Gene EDLL domain AP2 and AP2 or ID SEQ ID NO: ID amino acid EDLL domain AP2 and EDLL EDLL domain, NO: is derived (GID) coordinates SEQ ID NO: conserved domains respectively   24 Arabidopsis G1792 AP2: 2386 AP2: 100 thaliana  16-80 KQARFRGVRRRPWGKFAAEIRD PSRNGARLWLGTFETAEEAARA YDRAAFNLRGHLAILNFPNEY EDLL: 5128 EDLL: 100 117-132 VFEFEYLDDKVLEELL   26 Arabidopsis G1795 AP2: 2387 AP2: 69 thaliana  11-75 EHGKYRGVRRRPWGKYAAEIRD SRKHGERVWLGTFDTAEEAARA YDQAAYSMRGQAAILNFPHEY EDLL: 5129 EDLL: 93 104-119 VFEFEYLDDSVLEELL   66 Arabidopsis G30 AP2: 2411 AP2: 70 thaliana  16-80 EQGKYRGVRRRPWGKYAAEIRD SRKHGERVWLGTFDTAEDAARA YDRAAYSMRGKAAILNFPHEY EDLL: 5130 EDLL: 87 100-115 VFEFEYLDDSVLDELL 1850 Oryza G3383 AP2: 3475 AP2: 79 sativa   9-73 TATKYRGVRRRPWGKFAAEIRD PERGGARVWLGTFDTAEEAARA YDRAAYAQRGAAAVLNFPAAA EDLL: 5131 EDLL: 85 101-116 KIEFEYLDDKVLDDLL 1172 Arabidopsis G1791 AP2: 3064 AP2: 73 thaliana  10-74 NEMKYRGVRKRPWGKYAAEIRD SARHGARVWLGTFNTAEDAARA YDRAAFGMRGQRAILNFPHEY EDLL: 5132 EDLL: 81 108-123 VIEFEYLDDSLLEELL 1984 Glycine max G3519 AP2: 3558 AP2: 78  13-77 CEVRYRGIRRRPWGKFAAEIRD PTRKGTRIWLGTFDTAEQAARA YDAAAFHFRGHRAILNFPNEY EDLL: 5133 EDLL: 80 128-143 TFELEYLDNKLLEELL 1848 Oryza G3381 AP2: 3474 AP2: 76 sativa  14-78 LVAKYRGVRRRPWGKFAAEIRD SSRHGVRVWLGTFDTAEEAARA YDRSAYSMRGANAVLNFPADA EDLL: 5134 EDLL: 78 109-124 PIEFEYLDDHVLQEML 2104 Oryza G3737 AP2: 3627 AP2: 76 sativa   8-72 AASKYRGVRRRPWGKFAAEIRD PERGGSRVWLGTFDTAEEAARA YDRAAFAMKGAMAVLNFPGRT EDLL: 5135 EDLL: 78 101-116 KVELVYLDDKVLDELL 1976 Oryza G3515 AP2: 3554 AP2: 75 sativa  11-75 SSSSYRGVRKRPWGKFAAEIRD PERGGARVWLGTFDTAEEAARA YDRAAFAMKGATAMLNFPGDH EDLL: 5136 EDLL: 78 116-131 KVELECLDDKVLEDLL 1978 Zea mays G3516 AP2: 3555 AP2: 74   6-70 KEGKYRGVRKRPWGKFAAEIRD PERGGSRVWLGTFDTAEEAARA YDRAAFAMKGATAVLNFPASG EDLL: 5137 EDLL: 78 107-122 KVELECLDDRVLEELL 1986 Glycine max G3520 AP2: 3559 AP2: 80  14-78 EEPRYRGVRRRPWGKFAAEIRD PARHGARVWLGTFLTAEEAARA YDRAAYEMRGALAVLNFPNEY EDLL: 5138 EDLL: 75 109-124 VIEFECLDDKLLEDLL 1980 Zea mays G3517 AP2: 3556 AP2: 72  13-77 EPTKYRGVRRRPWGKYAAEIRD SSRHGVRIWLGTFDTAEEAARA YDRSANSMRGANAVLNFPEDA EDLL: 5139 EDLL: 75 103-118 VIEFEYLDDEVLQEML 1982 Glycine max G3518 AP2: 3557 AP2: 78  13-77 VEVRYRGIRRRPWGKFAAEIRD PTRKGTRIWLGTFDTAEQAARA YDAAAFHFRGHRAILNFPNEY EDLL: 5140 EDLL: 73 135-150 TFELEYFDNKLLEELL 2106 Zea mays G3739 AP2: 3628 AP2: 72  13-77 EPTKYRGVRRRPWGKYAAEIRD SSRHGVRIWLGTFDTAEEAARA YDRSAYSMRGANAVLNFPEDA EDLL: 5141 EDLL: 68 107-122 VIELEYLDDEVLQEML 1846 Oryza G3380 AP2: 3473 AP2: 77 sativa  18-82 ETTKYRGVRRRPSGKFAAEIRD SSRQSVRVWLGTFDTAEEAARA YDRAAYAMRGHLAVLNFPAEA EDLL: 5142 EDLL: 62 103-118 VIELECLDDQVLQEML 2132 Zea mays G3794 AP2: 3641 AP2: 73   6-70 EPTKYRGVRRRPSGKFAAEIRD SSRQSVRMWLGTFDTAEEAARA YDRAAYAMRGQIAVLNFPAEA EDLL: 5143 EDLL: 62 102-117 VIELECLDDQVLQEML

TABLE 24 Conserved domains of G913 (TF family: AP2) and closely related AP2 sequences Percent ID of conserved Species Conserved AP2 domain SEQ from which Gene AP2 domain Conserved to G913 ID SEQ ID NO: ID amino acid AP2 domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved AP2 domain AP2 domain Arabidopsis G913 62-128 2781 HSIFRGIRLRNGKWVSEIREPR 100 thaliana KYITRIWLGTYPVPEMAAAAYD VAALALKGPDAVLNFPGLALTY VA Arabidopsis G2514 16-82 3277 DPVYRGIRCRSGKWVSEIREPR 84 thaliana KTTRIWLGTYPMAEMAAAAYDV AAMALKGREAVLNFPGSVGSYP V Arabidopsis G976 87-153 2806 NPVYRGIRCRSGKWVSEIREPK 82 thaliana KTTRVWLGTYPTPEMAAAAYDV AALALKGGDTLLNFPDSLGSYP I Arabidopsis G1753 12-80 3046 HPLYRGVRQRKNSNKWVSEIR 70 thaliana EPRKPNRIWLGTFSTPEMAAI AYDVAALALKGSQAELNFPNS VSSLPA

TABLE 25 Conserved domains of G1073 (TF family: AT-hook) and closely related AT-hook sequences Percent ID of conserved Conserved AT-hook or AT-hook 2nd domain and 2nd AT-hook to G1073 Species conserved and 2nd AT-hook or SEQ from which Gene domain conserved 2nd conserved ID SEQ ID NO: ID amino acid domain AT-hook and 2nd domains, NO: is derived (GID) coordinates SEQ ID NO: conserved domains respectively   18 Arabidopsis G1073 AT-hook: 2382 AT-hook: 100 thaliana  63-71 RRPRGRPAG 2nd domain: 2383 2nd conserved domain: 100  71-216 GSKNKPKPPTIITRDSPNVLRSHV LEVTSGSDISEAVSTYATRRGCGV CIISGTGAVTNVTIRQPAAPAGGG VITLHGRFDILSLTGTALPPPAPP GAGGLTVYLAGGQGQVVGGNVAGS LIASGPVVLMAASFANAVYDRLPI EE 5145 Oryza G3406 AT-hook: 5146 AT-hook: 100 sativa  82-90 RRPRGRPPG 2nd domain: 5147 2nd conserved domain: 70  90-232 GSKNKPKPPVIITRiESANTLRAH ILEVGSGCDVFECVSTYARRRQRG VCVLSGSGVVTNVTLRQPSAPAGA VVSLHGRFEILSLSGSFLPPPAPP GATSLTIFLAGGQGQVVGGNVVGA LYAAGPVIVIAASFANVAYERLPL 1870 Oryza G3399 AT-hook: 3488 AT-hook: 100 sativa  99-107 RRPRGRPPG 2nd domain: 3489 2nd conserved domain: 71 107-253 GSKNKPKPPIIVTRDSPNALHSHV LEVAGGADVVDCVAEYARRRGRGV CVLSGGGAVVNVALRQPGASPPGS MVATLRGRFEILSLTGTVLPPPAP PGASGLTVFLSGGQGQVIGGSVVG PLVAAGPVVLMAASFANAVYERLP LEG  798 Arabidopsis G1067 AT-hook: 2852 AT-hook: 85 thaliana  86-94 KRPRGRPPG 2nd domain: 2853 2nd conserved domain: 71  94-247 GSKNKAKPPIIVTRDSPNALRSHV LEVSPGADIVESVSTYARRRGRGV SVLGGNGTVSNVTLRQPVTPGNGG GVSGGGGVVTLHGRFEILSLTGTV LPPPAPPGAGGLSIFLAGGQGQVV GGSVVAPLIASAPVILMAASFSNA VFERLPIEE 1918 Glycine max G3459 AT-hook: 3523 AT-hook: 100  77-85 RRPRGRPPG 2nd domain: 3524 2nd conserved domain: 67  85-228 GSKNKPKPPVIITRESANTLRAHI LEVGSGSDVFDCVTAYARRRQRGI CVLSGSGTVTNVSLRQPAAAGAVV TLHGRFEILSLSGSFLPPPAPPGA TSLTIYLAGGQGQVVGGNVIGELT AAGPVIVIAASFTNVAYERLPLEE 1872 Oryza G3400 AT-hook: 3490 AT-hook: 100 sativa  83-91 RRPRGRPLG 2nd domain: 3491 2nd conserved domain: 69  91-237 GSKNKPKPPIIVTRDSPNAFHSHV LEVAAGTDIVECVCEFARRRGRGV SVLSGGGAVANVALQGSPPGSLVA TMRGQFEILSLTGTVLPPPAPPSA SGLTVFLSGGQGQVVGGSVAGQLI AAGPVFLMAASFANAVYERLPLDG 1694 Arabidopsis G2789 AT-hook: 3364 AT-hook: 100 thaliana  59-67 RRPRGRIPAG 2nd domain: 3365 2nd conserved domain: 65  67-208 GSKNKPKAPIIVTRDSANAFRCHV MEITNACDVMESLAVFARRRQRGV CVLTGNGAVTNVTVRQPGGGVVSL HGRFELLSLSGSFLPPPAPPAASG LKVYLAGGQGQVIGGSVVGPLTAS SPVVVMAASFGNASYERLPLEE 1920 Glycine max G3460 AT-hook: 3525 AT-hook: 100  74-82 RRPRGRPSG 2nd domain: 3526 2nd conserved domain: 67  82-225 GSKNKPKPPVIITRESANTLRAHI LEVGSGSDVFDCVTAYARRRQRGI CVLSGSGTVTNVSLRQPAAAGAVV RLHGRFEILSLSGSFLPPPAPPGA TSLTIYLAGGQGQVVGGNVVGELT AAGPVIVIAASFTNVAYERLPLEE 1116 Arabidopsis G1667 AT-hook: 3035 AT-hook: 85 thaliana  53-61 KRPRGRPAG 2nd domain: 5148 2nd conserved domain: 65  61-204 GSKNKPKPPIIVTHDSPNSLRANA VEISSGCDICETLSDFARRKQRGL CILSANGCVTNVTLRQPASSGAIV TLHGRYEILSLLGSILPPPAPLGI TGLTIYLAGPQGQVVGGGVVGGLI ASGPVVLMAASFMNAVFDRLPMDD 1424 Arabidopsis G2156 AT-hook: 3205 AT-hook: 85 thaliana  72-80 KRPRGRPPG 2nd domain: 3206 2nd conserved domain: 68  80-232 GSKNKPKPPVIVTRDSPNVLRSHV LEVSSGADIVESVTTYARRRGRGV SILSGNGTVANVSLRQPATTAAHG ANGGTGGVVALHGRFEILSLTGTV LPPPAPPGSGGLSIFLSGVQGQVI GGNVVAPLVASGPVILMAASFSNA TFERLPLED 1916 Glycine max G3456 AT-hook: 3521 AT-hook: 100  44-52 RRPRGRPPG 2nd domain: 3522 2nd conserved domain: 64  52-195 GSRNKPKPPIFVTRDSPNALRSHV MEIAVGADIADCVAQFARRRQRGV SILSGSGTVVNVNLRQPTAPGAVM ALHGRFDILSLTGSFLPGPSPPGA TGLTIYLAGGQGQIVGGGVVGPLV AAGPVLVMAATFSNATYERLPLED 1876 Oryza G3407 AT-hook: 3494 AT-hook: 100 sativa  63-71 RRPRGRPPG 2nd domain: 3495 2nd conserved domain: 64  71-220 GSKNKPKPPVIITRESANALRAHI LEVAAGCDVFEALTAYARRRQRGV CVLSAAGTVANVTLRQPQSAQPGP ASPAVATLHGRFEILSLAGSFLPP PAPPGATSLAAFLAGGQGQVVGGS VAGALIAAGPVVVVAASFSNVAYE RLPLED 1874 Oryza G3401 AT-hook: 3492 AT-hook: 100 sativa  35-43 RRIPRGRPPG 2nd domain: 3493 2nd conserved domain: 64  43-186 GSKNKPKPPIFVTRDSPNALRSHV MEVAGGADVAESIAHFARRRQRGV CVLSGAGTVTDVALRQPAAPSAVV ALRGRFEILSLTGTFLPGPAPPGS TGLTVYLAGGQGQVVGGSVVGTLT AAGPVMVIASTFANATYERLPLDQ 1420 Arabidopsis G2153 AT-hook: 3202 AT-hook: 100 thaliana  80-88 RRPRGRPAG 2nd domain: 3203 2nd conserved domain: 63  88-239 GSKNKPKPPIFVTRDSPNALKSHV MEIASGTDVIETLATFARRRQRGI CILSGNGTVANVTLRQPSTAAVAA APGGAAVLALQGRFEILSLTGSFL PGPAPPGSTGLTIYLAGGQGQVVG GSVVGPLMAAGPVMLIAATFSNAT YERLPLEE  802 Arabidopsis G1069 AT-hook: 2855 AT-hook: 100 thaliana  67-75 RRPRGRPPG 2nd domain: 5149 2nd conserved domain: 63  75-218 GSKNKPKAPIFVTRDSPNALRSHV LEISDGSDVADTIAHFSRRRQRGV CVLSGTGSVANVTLRQAAAPGGVV SLQGRFEILSLTGAFLPGPSPPGS TGLTVYLAGVQGQVVGGSVVGPLL AIGSVMVIAATFSNATYERLPMEE 2034 Oryza G3556 AT-hook: 3583 AT-hook: 100 sativa  45-53 RRPRGRPPG 2nd domain: 3584 2nd conserved domain: 64  53-196 GSKNKPKPPVVVTRESPNAMRSHV LEIASGADIVEAIAGFSRRRQRGV SVLSGSGAVTNVTLRQPAGTGAAA VALRGRFEILSMSGAFLPAPAPPG ATGLAVYLAGGQGQVVGGSVMGEL IASGPVMVIAATFGNATYERLPLD 1426 Arabidopsis G2157 AT-hook: 3207 AT-hook: 100 thaliana  88-96 RRPRGRPPG 2nd domain: 3208 2nd conserved domain: 61 GSKNKPKSPVVVTKESPNSLQSHV LEIATGADVAESLNAFARRRGRGV SVLSGSGLVTNVTLRQPAASGGVV SLRGQFEILSMCGAFLPTSGSPAA AAGLTIYLAGAQGQVVGGGVAGPL IASGPVIVIAATFCNATYERLPIE E 1878 Oryza G3408 AT-hook: 3496 AT-hook: 57 sativa  82-90 KKRRGRPPG 2nd domain: 3497 2nd conserved domain: 45  90-247 GSKNKPKPPVVITREAEPAAAMRP HVIEIPGGRDVAEALARFSSRRNL GICVLAGTGAVANVSLRHPSPGVP GSAPAAIVFHGRYEILSLSATFLP PAMSSVAPQAAVAAAGLSISLAGP HGQIVGGAVAGPLYAATTVVVVAA AFTNPTFHRLPADD

TABLE 26 Conserved domains of G1274 (TF family: WRKY) and closely related WRKY sequences Percent ID of conserved Species Conserved WRKY domain SEQ from which Gene WRKY domain Conserved to G1274 ID SEQ ID NO: ID amino acid WRKY domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved WRKY domain WRKY domain   20 Arabidopsis G1274 110-166 2384 DDGFKWRKYGKKSVKNNINK 100 thaliana RNYYKCSSEGCSVKKRVERD GDDAAYVITTYEGVHNH 2090 Glycine max G3724 107-163 3620 DDGYKWRKYGKKSVKSSPNL 84 RNYYKCSSGGCSVKKRVERD RDDYSYVITTYEGVHNH 2098 Zea mays G3728 108-164 3624 DDGFKWRKYGKKAVKNSPNP 82 RNYYRCSSEGCGVKKRVERD RDDPRYVITTYDGVHNH 2136 Zea mays G3804 108-164 3643 DDGFKWRKYGKKAVKNSPNP 82 RNYYRCSSEGCGVKKRVERD RDDPRYVITTYDGVHNH 2134 Glycine max G3803 111-167 3642 DDGYKWRKYGKKTVKNNPNP 80 RNYYKCSGEGCNVKKRVERD RDDSNYVLTTYDGVHNH 2096 Zea mays G3727 102-158 3623 DDGFKWRKYGKKAVKSSPNP 80 RNYYRCSSEGCGVKKRVERD RDDPRYVITTYDGVHNH 2084 Oryza G3721  96-152 3617 DDGFKWRKYGKKAVKNSPNP 78 sativa RNYYRCSTEGCNVKKRVERD REDHRYVITTYDGVHNH 2086 Zea mays G3722 129-185 3618 DDGYKWRKYGKKSVKNSPNP 78 RNYYRCSTEGCNVKKRVERD RDDPRYVVTMYEGVHNH 2094 Oryza G3726 135-191 3622 DDGYKWRKYGKKSVKNSPNP 78 sativa RNYYRCSTEGCNVKKRVERD KDDPSYVVTTYEGTHNH 2082 Zea mays G3720 135-191 3616 DDGYKWRKYGKKSVKNSPNP 78 RNYYRCSTEGCNVKKRVERD KDDPSYVVTTYEGMHNH 2088 Glycine max G3723 112-168 3619 DDGYKWRKYGKKTVKSSPNP 77 RNYYKCSGEGCDVKKRVERD RDDSNYVLTTYDGVHNH 2080 Arabidopsis G1275 113-169 2908 DDGFKWRKYGKKMVKNSPHP 77 thaliana RNYYKCSVDGCPVKKRVERD RDDPSFVITTYEGSHNH 2102 Oryza G3730 107-163 3626 DDGFKWRKYGKKAVKSSPNP 77 sativa RNYYRCSAAGCGVKKRVERD GDDPRYVVTTYDGVHNH 2080 Zea mays G3719  98-154 3615 DDGFKWRKYGKKTVKSSPNP 77 RNYYRCSAEGCGVKKRVERD SDDPRYVVTTYDGVHNH 2092 Oryza G3725 158-214 3621 DDGYKWRKYGKKSVKNSPNP 75 sativa RNYYRCSTEGCNVKKRVERD KNDPRYVVTMYEGIHNH 2100 Oryza G3729 137-193 3625 DDGYRWRKYGKKMVKNSPNP 75 sativa RNYYRCSSEGCRVKKRVERA RDDARFVVTTYDGVHNH

TABLE 27 Conserved domains of G2999 (TF family: ZF-HD) and closely related ZF-HD sequences Percent ID of conserved ZF or HD domain Conserved to G2999 Species HD and ZF Conserved conserved SEQ from which Gene domain HD and ZF ZF or HD ID SEQ ID NO: ID amino acid domain domains, NO: is derived (GID) coordinates SEQ ID NO: ZF and HD conserved domains respectively 1794 Arabidopsis G2999 ZF: 3436 ZF: 100 thaliana  80-133 ARYRECQKNHAASSGGHVVDGC GEFMSSGEEGTVESLLCAACDC HRSFHRKEID HD: 3437 HD: 100 198-261 KKRFRTKFNEEQKEKMMEFAEK IGWRMTKLEDDEVNRFCREIKV KRQVFKVWMHNNKQAAKKKD 1792 Arabidopsis G2998 ZF: 3434 ZF: 81 thaliana  74-127 VRYRECLKNHAASVGGSVHDGC GEFMPSGEEGTIEALRCAACDC HRNFHRKEMD HD: 3435 HD: 72 240-303 KKRFRTKFTITDQKERMMDFAE KLGWRMNKQDEEELKRFCGEIG VKRQVFKVWMHNNKNNAKKPP 1796 Arabidopsis G3000 ZF: 3438 ZF: 79 thaliana  58-111 AKYRECQKNHAASTGGHVVDGC CEFMAGGEEGTLGALKCAACNC HRSFHRKEVY HD: 3439 HD: 65 181-244 KKRVRTKINEEQKEKMKEFAER LGWRMQKKDEEEIDKFCRMVNL RRQVFKVWMHNNKQAMKRNN 2074 Oryza G3690 ZF: 3611 ZF: 70 sativa 161-213 WRYRECLKNHAARMGAHVLDGC GEFMSSPGDGAAALACAACGCH RSFHRREPA HD: 3612 HD: 59 318-381 KKRFRTKFTAEQKERMREFAHR VGWRIIHKPDAAAVDAFCAQVG VSRRVLKVWMHNNKHLAKTPP 1790 Arabidopsis G2997 ZF: 3432 ZF: 69 thaliana  47-100 IRYRECLKNHAVNIGGHAVDGC CEFMPSGEDGTLDALKCAACGC HRNFHRKETE HD: 3433 HD: 61 157-220 TKRFRTKFTAEQKEKMLAFAER LGWRIQKHDDVAVEQFCAETGV RRQVLKIWMHNNKNSLGKKP 2062 Zea mays G3676 ZF: 3599 ZF: 69  40-89 ARYHECLRNHAAALGGHVVDGC GEFMPGDGDSLKCAACGCHRSF HRKDDA HD: 3600 HD: 57 162-225 RKRFRTKFTPEQKEQMLAFAER LGWRLQKQDDALVQHFCDQVGV RRQVFKVWMHNNKHTGRRQQ 2072 Oryza G3686 ZF: 3609 ZF: 68 sativa  38-88 CRYHECLRNHAAASGGHVVDGC GEFMPASTEEPLACAACGCHRS FHRRDPS HD: 3610 HD: 50 159-222 RRRSRTTFTREQKEQMLAFAER VGWRIQRQEEATVEHFCAQVGV RRQALKVWMHNNKHSFKQKQ 1788 Arabidopsis G2996 ZF: 3430 ZF: 67 thaliana  73-126 FRFRECLKNQAVNIGGHAVDGC GEFMPAGIEGTIDALKCAACGC HRNFHRKELP HD: 3431 HD: 54 191-254 RKRHRTKFTAEQKERMLALAER IGWRIQRQDDEVIQRFCQETGV PRQVLKVWLHNNKHTLGKSP 5151 Arabidopsis G3001 ZF: 5152 ZF: 63 thaliana  62-113 PHYYECRKNHAADIGTTAYDGC GEFVSSTGEEDSLNCAACGCHR NFHREELI HD: 5153 HD: 48 179-242 VKRLKTKFTAEQTEKMRDYAEK LRWKVRPERQEEVEEFCVEIGV NRKNFRIWMNNHKDKIIIDE 2070 Oryza G3685 ZF: 3607 ZF: 62 sativa  43-95 VRYHECLRNHAAAMGGHVVDGC REFMPMPGDAADALKCAACGCH RSFHRKDDG HD: 3608 HD: 61 171-235 RKRFRTKFTPEQKEQMLAFAER VGWRMQKQDEALVEQFCAQVGV RRQVFKVWMHNNKSSIGSSS 1784 Arabidopsis G2993 ZF: 3426 ZF: 62 thaliana  85-138 IKYKECLKNHAATMGGNAIDGC GEFMPSGEEGSIEALTCSVCNC HRNFHRRETE HD: 3427 HD: 58 222-285 KKRFRTKFTQEQKEKMISFAER VGWKIQRQEESVVQQLCQEIGI RRRVLKVWMHNNKQNLSKKS 2066 Zea mays G3681 ZF: 3603 ZF: 62  22-77 PLYRECLKNHAASLGGHAVDGC GEFMPSPGANPADPTSLKCAAC GCHRNFHRRTVE HD: 3604 HD: 54 208-271 RKRFRTKFTAEQKQRMQELSER LGWRLQKRDEAVVDEWCRDMGV GKGVFKVWMHNNKHNFLGGH 1776 Arabidopsis G2989 ZF: 3418 ZF: 61 thaliana  50-105 VTYKECLKNHAAAIGGHALDGC GEFMPSPSSTPSDPTSLKCAAC GCHRNFHRRETD HD: 3419 HD: 62 192-255 RKRFRTKFSSNQKEKMHEFADR IGWKIQKRDEDEVRDFCREIGV DKGVLKVWMHNNKNSFKFSG 1780 Arabidopsis G2991 ZF: 3422 ZF: 60 thaliana  54-109 ATYKECLKNHAAGIGGHALDGC GEFMPSPSFNSNDPASLTCAAC GCHRNFHRREED HD: 3423 HD: 66 179-242 RKRFRTKFSQYQKEKMFEFSER VGWRMPKADDVVVKEFCREIGV DKSVFKVWMHNNKISGRSGA 1778 Arabidopsis G2990 ZF: ZF: 59 thaliana  54-109 FTYKECLKNHAAALGGHALDGC GEFMPSPSSISSDPTSLKCAAC GCHRNFHRRDPD HD: HD: 57 200-263 RKRFRTKFSQFQKEKMHEFAER VGWKMQKRDEDDVRDFCRQIGV DKSVLKVWMHNNKNTFNRRD 1782 Arabidopsis G2992 ZF: ZF: 59 thaliana  29-84 VCYKECLKNHAANLGGHALDGC GEFMPSPTATSTDPSSLRCAAC GCHRNFHRRDPS HD: HD: 54 156-219 RKRTRTKFTPEQKIKMRAFAEK AGWKINGCDEKSVREFCNEVGI ERGVLKVWMHNNKYSLLNGK 5155 Arabidopsis G2995 ZF: 5156 ZF: 54 thaliana   3-58 VLYNECLKNHAVSLGGHALDGC GEFTPKSTTILTDPPSLRCDAC GCHRNFHRRSPS HD: 5157 HD: 50 115-178 KKHKRTKFTAEQKVKMRGFAER AGWKINGWDEKWVREFCSEVGI ERKVLKVWIHNNKYFNNGRS 1798 Arabidopsis G3002 ZF: 3440 ZF: 49 thaliana   5-53 CVYRECMRNHAAKLGSYAIDGC REYSQPSTGDLCVACGCHRSYH RRIDV HD: 3441 HD: 38 106-168 QRRRKSKFTAEQREAMKDYAAK LGWTLKDKRALREEIRVFCEGI GVTRYHFKTWVNNNKKFYH

TABLE 28 Conserved domains of G3086 (TF family:HLH/MYC) and closely related HLH/MYC sequences Percent ID of conserved Species Conserved bHLH domain SEQ from which Gene bHLH domain Conserved to G3086 ID SEQ ID NO: ID amino acid bHLH domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved bHLH domain bHLH domain 1836 Arabidopsis G3086 307-365 3468 KRGCATHPRSIAERVRRTKLS 100 thaliana ERMRKLQDLVPNMDTQTNTAD MLDLAVQYIKDLQEQVK 2126 Glycine max G3768 190-248 3638 KRGCATHPRSIAERVRRTKIS 93 ERMRKLQDLVPNMDKQTNTAD MLDLAVDYIKDLQKQVQ 2128 Glycine max G3769 240-298 3639 KRGCATHPRSIAERVRRTKIS 93 ERMRKLQDLVPNMDKQTNTAD MLDLAVEYIKDLQNQVQ 2124 Glycine max G3767 146-204 3637 KRGCATHPRSIAERVRRTKIS 93 ERMRKLQDLVPNMDKQTNTAD MLDLAVDYIKDLQKQVQ 2110 Oryza G3744  71-129 3630 KRGCATHPRSIAERVRRTRIS 89 sativa ERIRKLQELVPNMDKQTNTAD MLDLAVDYIKDLQKQVK 2116 Zea mays G3755  97-155 3633 KRGCATHPRSIAERVRRTKIS 89 ERIRKLQELVPNMDKQTNTSD MLDLAVDYIKDLQKQVK 2122 Glycine max G3766  35-93 3636 KRGCATHPRSIAERVRRTRIS 88 ERMRKLQELVPHMDKQTNTAD MLDLAVEYIKDLQKQFK  472 Arabidopsis G592 282-340 2656 KRGCATHPRSIAERVRRTRIS 88 thaliana ERMRKLQELVPNMDKQTNTSD MLDLAVDYIKDLQRQYK 2108 Oryza G3742 199-257 3629 KRGCATHPRSLAERVRRTRIS 86 sativa ERIRKLQELVPNMEKQTNTAD MLDLAVDYIKELQKQVK 2112 Oryza G3746 312-370 3631 KRGCATHPRSIAERERRTRIS 79 sativa KRLKKLQDLVPNMDKQTNTSD MLDIAVTYIKELQGQVE 2130 Glycine max G3771  84-142 3640 KRGCATHPRSIAERVRRTRIS 79 DRIRKLQELVPNMDKQTNTAD MLDEAVAYVKFLQKQIE 2120 Glycine max G3765 147-205 3635 KRGFATHPRSIAERVRRTRIS 79 ERIRKLQELVPTMDKQTSTAE MLDLALDYIKDLQKQFK  834 Arabidopsis G1134 187-245 2874 KRGCATHPRSIAERVRRTRIS 77 thaliana DRIRKLQELVPNMDKQTNTAD MLEEAVEYVKVLQRQIQ 1570 Arabidopsis G2555 184-242 3291 KRGCATHPRSIAERVRRTRIS 76 thaliana DRIRRLQELVPNMDKQTNTAD MLEEAVEYVKALQSQIQ 5159 Arabidopsis G2149 286-344 5160 KRGCATHPRSIAERERRTRIS 74 thaliana GKLKKLQDLVPNMDKQTSYSD MLDLAVQHIKGLQHQLQ 1676 Arabidopsis G2766 234-292 3353 KRGFATHPRSIAERERRTRIS 72 thaliana GKLKKLQELVPNMDKQTSYAD MLDLAVEHIKGLQHQVE 2118 Zea mays G3760 243-300 3634 RRGQATDPHSIAERLRRERIA 59 ERMKALQELVPNANKTDKASM LDEIVDYVKFLQLQVK 2114 Oryza G3750 136-193 3632 RRGQATDPHSIAERLRRERIA 57 sativa ERMRALQELVPNTNKTDRAAM LDEILDYVKFLRLQVK

TABLE 29 Conserved domains of G1988 (TF family: Z-CONSTANS-like) and closely related Z-CO-like sequences Percent ID of conserved Species Conserved B-box domain SEQ from which Gene B-box domain Conserved to G1988 ID SEQ ID NO: ID amino acid B-box domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved B-box domain B-box domain   30 Arabidopsis G1988  5-50 2389 CELCGAEADLHCAADSAFLCRSCD 100 thaliana AKFHASNFLFARHFRRVICPNC 2348 Zea mays G4297 14-55 3761 CELCGGAAAVHCAADSAFLCPRCD 70 AKVHGANFLASRHVRRRL 2262 Oryza G4012 15-56 3718 CELCGGVAAVHCAADSAFLCLVCD 67 sativa DKVHGANFLASRHRRRRL 2350 Oryza G4298 15-56 3762 CELCGGVAAVHCAADSAFLCLVCD 67 sativa DKVHGANFLASRHPRRRW 2250 Zea mays G4000 20-61 3712 CELCGGAAAVHCAADSAFLCLRCD 70 AKVHGANFLASRHVRRRL 2260 Oryza G4011  8-49 3717 CALCGAAAAVHCEADAAFLCAACD 65 sativa AKVHGANFLASRHHRRRV 2254 Glycine max G4005  6-51 3714 CELCDQQASLYCPSDSAFLCSDCD 60 AAVHAANFLVARHLRRLLCSKC 2252 Glycine max G4004  6-51 3713 CELCHQLASLYCPSDSAFLCFHCD 60 AAVHAANFLVARHLRRLLCSKC 2256 Citrus G4007  5-50 3715 CELCSQEAALHCASDEAFLCFDCD 58 sinensis DRVHKANFLVARHVRQTLCSQC 2352 Solanum G4299  9-54 3763 CELCNDQAALFCPSDSAFLCFHCD 58 lycopersicum AKVHQANFLVARHLRLTLCSHC 2258 Populus G4009  6-51 3716 CELCKGEAGVYCDSDAAYLCFDCD 56 trichocarpa SNVHNANFLVARHIRRVICSGC

TABLE 30 Conserved domains of G922 (TF family: SCR) and closely related SCR sequences Percent ID SCR of conserved Species Conserved SCR domain SEQ from which Gene domain Conserved to G922 ID SEQ ID NO: ID amino acid domain conserved NO: is derived (GID) coordinates SEQ ID NO: SCR conserved domains SCR domain  690 Arabidopsis G922 1st 2785 1st SCR domain: 100 thaliana 134-199 RRLFFEMFPILKVSYLLTNRAI LEAMEGEKMVHVIDLDASEPAQ WLALLQAFNSRPEGPPHLRITG 2nd: 2786 2nd SCR domain: 100 332-401 FLNAIWGLSPKVMVVTEQDSDH NGSTLMERLLESLYTYAALFDC LETKVPRTSQDRIKVEKMLFGE EIKN 3rd: 3787 3rd SCR domain: 100 405-478 CEGFERRERHEKLEKWSQRIDL AGFGNVPLSYYAMLQARRLLQG CGFDGYRIKEESGCAVICWQDR PLYSVSAW 2146 Solanum G3824 1st: 3656 1st SCR domain: 69 lycopersicum  42-107 RKMFFEIFPFLKVAFVVTNQAI IEAMEGEKMVHIVDLNAAEPLQ WRALLQDLSARPEGPPHLRITG 2nd: 3657 2nd SCR domain: 78 235-304 FLNALWGLSPKVMVVTEQDANH NGTFLMERLSESLHFYAALFDC LESTLPRTSLERLKVEKMLLGE EIRN 3rd: 3658 3rd SCR domain: 77 308-381 CEGIERKERHEKLEKWFQRFDT SGFGNVPLSYYAMLQARRLLQS YSCEGYKIKEDNGCVVICWQD RPLFSVSSW 2140 Glycine max G3811 1st: 3647 1st SCR domain: 68 103-168 QKLFFELLPFLKFSYILTNQAI VEAMEGEKMVHIVDLYGAGPAQ WISLLQVLSARPEGPPHLRITG 2nd: 3648 2nd SCR domain: 74 296-365 FLNALWGLSPKVMVVTEQDFNH NCLTMMERLAEALFSYAAYFDC LESTVSRASMDRLKLEKMLFGE EIKN 3rd: 3649 3rd SCR domain: 60 369-442 CEGCERKERHEKMDRWIQRLD LSGFANVPISYYGMLQGRRFL QTYGCEGYKMREECGRVMICW QERSLFSITAW 2138 Glycine max G3810 1st: 3644 1st SCR domain: 66 106-171 QKLFFELFPFLKVAFVLTEQA IIEAMEGEKVIHIIDLNAAEA AQWIALLRVLSAHPEGPPHLR ITG 2nd: 3645 2nd SCR domain: 80 305-374 FLNALWGLSPKVMVVTEQDCN HNGPTLMDRLLEALYSYAALF DCLESTVSRTSLERLRVEKML FGEEIKN 3rd: 3646 3rd SCR domain: 71 378-451 CEGSERKERHEKLEKWFQRFD LAGFGNVPLSYFGMVQARRFL QSYGCEGYRMRDENGCVLICW EDRPMYSISAW 2144 Oryza G3814 1st: 3653 1st SCR domain: 61 sativa 123-190 RRHMFDVLPFLKLAYLTTNHA ILEAMEGERFVHVVDFSGPAA NPVQWIALFHAFRGRREGPPH LRITA 2nd: 3654 2nd SCR domain: 49 332-400 FLSAVRSLSPKIMVMTEQEAN HNGGAFQERFDEALNYYASLF DCLQRSAAAAAERARVERVLL GEEIRG 3rd: 3655 3rd SCR domain: 46 404-480 CEGAERVERHERARQWAARME AAGMERVGLSYSGAMEARKLL QSCGWAGPYEVRHDAGGHGFF FCWHKRPLYAVTAW 2142 Oryza G3813 1st: 3650 1st SCR domain: 53 sativa 129-194 RRHFLDLCPFLRLAGAAANQS ILEAMESEKIVHVIDLGGADA TQWLELLHLLAARPEGPPHLR LTS 2nd: 3651 2nd SCR domain: 61 290-359 FLGALWGLSPKVMVVAEQEAS HNAAGLTERFVEALNYYAALF DCLEVGAARGSVERARVERWL LGEEIKN 3rd: 3652 3rd SCR domain: 64 363-436 CDGGERRERHERLERWARRLE GAGFGRVPLSYYALLQARRVA QGLGCDGFKVREEKGNFFLCW QDRALFSVSAW 2148 Oryza G3827 n/a n/a (no corresponding 1st n/a sativa SCR domain) 2nd: 3659 2nd SCR domain: 56 226-295 DVESLRGLSLKVMVVTEQEVS HNAAGLTERFVEALNYYAALF DCLEVGGARGSVERTRVERWL LGEEIKN 3rd: 3660 3rd SCR domain: 62 299-365 CDGGERRERHERLEGAGFGRV PLSYYALLQARRVAQGLGCDG FKVREEKGNFFLCWQDRALFS VSAW

TABLE 31 Conserved domains of G1760 (TF family: MADS) and closely related MADS box sequences Percent ID of conserved MADS DNA binding Conserved domain MADS DNA Conserved to G1760 Species binding MADS DNA conserved SEQ from which Gene domain binding MADS DNA ID SEQ ID NO: ID amino acid domain Conserved MADS DNA binding NO: is derived (GID) coordinates SEQ ID NO: binding domain domain   22 Arabidopsis G1760 2-57 2385 GRGKIVIQRIDDSTSRQVTF 100 thaliana SKRRKGLIKKAKELAILCDA EVGLIIFSSTGKLYDF  110 Arabidopsis G152 2-57 2433 GRGKIVIQKIDDSTSRQVTF 92 thaliana SKRRKGLIKKAKELAILCDA EVCLIIFSNTDKLYDF 5162 Antirrhinum G3982 2-57 5163 GRGKIVIQRIDKSTSRQVTF 89 majus SKRRSGLLKKAKELAILCDA EVGVVIFSSTGKLYEF 1950 Glycine max G3485 2-57 3541 GRGKLVIRRIDNSTSRQVTF 89 SKRRNGLLKKAKELAILCDA EVGVMIFSSTGKLYDF 2246 Glycine max G3980 2-57 3710 GRGKIVIRRIDNSTSRQVTF 89 SKRRNGLLKKAKELAILCDA EVGVMIFSSTGKLYDF 2248 Glycine max G3981 2-57 3711 GRGKIVIRRIDNSTSRQVTF 89 SKRRNGLLKKAKELAILCDA EVGVMIFSSTGKLYDF  112 Arabidopsis G153 2-57 2434 GRGKIVIRRIDNSTSRQVTF 87 thaliana SKRRSGLLKKAKELSILCDA EVGVIIFSSTGKLYDY  640 Arabidopsis G860 2-57 2756 GRGKIAIKRINNSTSRQVTF 85 thaliana SKRRNGLLKKAKELAILCDA EVGVIIFSSTGRLYDF 1938 Oryza G3479 2-57 3535 GRGKIVIRRIDNSTSRQVTF 83 sativa SKRRNGIFKKAKELAILCDA EVGLVIFSSTGRLYEY 1940 Oryza G3480 2-57 3536 GRGKIVIRRIDNSTSRQVTF 83 sativa SKRRNGIFKKAKELAILCDA EVGLMIFSSTGRLYEY 1942 Oryza G3481 2-57 3537 GRGKIVIRRIDNSTSRQVTF 83 sativa SKRRNGLLKKAKELSILCDA EVGLVVFSSTGRLYEF 1956 Zea mays G3489 2-57 3544 GRGKIVIRRIDNSTSRQVTF 83 SKRRNGIFKKAKELAILCDA EVGLVIFSSTGRLYEY 1948 Glycine max G3484 2-57 3540 GRGKIAIRRIDNSTSRQVTF 82 SKRRNGLLKKARELSILCDA EVGLMVFSSTGKLYDY 1952 Zea mays G3487 2-57 3542 GRGKIEIKRIDNATSRQVTF 82 SKRRGGLFKKAKELAILCDA EVGLVVFSSTGRLYHF 1954 Zea mays G3488 2-57 3543 GRGKIVIRRIDNSTSRQVTF 82 SKRRNGIFKKARELAILCDA EVGLVIFSSTGRLYEY 1946 Oryza G3483 2-57 3539 GRGKIEIKRIDNATSRQVTF 78 sativa SKRRSGLFKKARELSILCDA EVGLLVFSSTSRLYDF

TABLE 32 Conserved domains of G2053 (TF family: NAC) and closely related NAC sequences Percent ID of conserved Species Conserved NAC domain SEQ from which Gene NAC domain Conserved to G2053 ID SEQ ID NO: ID amino acid NAC domain conserved NO: is derived (GID) coordinates SEQ ID NO: Conserved NAC domain NAC domain 1336 Arabidopsis G2053 6-152 GLRFRPTDKEIVVDYLRPKNSD 100 thaliana RDTSHVDRVISTVTIRSFDPWE LPGQSRIKLKDESWCFFSPKEN KYGRGDQQIRKTKSGYWKITGK PKPILRNRQEIGEKKVLMFYMS KELGGSKSDWVMHEYHAFSPTQ MMMTYTICKVMFKGD  410 Arabidopsis G515 6-149 2624 GLRFCPTDEEIVVDYLWPKNSD 79 thaliana RDTSHVDRFINTVPVCRLDPWE LPGQSRIKLKDVAWCFFRLPKE NKYGRGDQQMRKTKSGFWKSTG RPKPIMRNRQQIGEKKILMFYT SKESKSDWVIHEYHGFSHNQMM MTYTLGKVMFNGG  412 Arabidopsis G516 6-141 2625 GFRFRPTDGEIVDIYLRPKNLE 64 thaliana SNTSHVDEVISTVDICSFDPWD LPSHSRMKTRDQVWYFFGRKEN KYGKGDRQIRKTKSGFWKKTGV TMDIMRKTGDREKIGEKRVLVF KNHGGSKSDWAMHEYHATFSSP NQGE  414 Arabidopsis G517 6-153 2626 GFRFRPNDEEIVDHYLRPKNLD 63 thaliana SDTSHVDEVISTVDICSFEPWD LPSKSMIKSRDGVWYFFSVKEM KYNRGDQQRRRTNSGFWKKTGK TMTVMRKRGNREKIGEKRVLVF KNRDGSKTDWVMHEYHATSLFP NQMMTYTVCKVEFKGE

TABLE 33 Conserved domains of miscellaneous sequences Species/ % ID to GID No., Domain in conserved domain SEQ Accession Gene Amino Acid of canonical ID No., or ID Coordinates SEQ ID NO: sequence NO: Identifier (GID) (Family) of Domain Conserved Domain (canonical GID)   40 Arabidopsis G7 AP2: 62-128 2395 PKKYRGVRQRPWGKWAAEIR 100% (G7) thaliana DPHKATRVWLGTFETAEAAA RAYDAAALRFRGSKAKLNFP ENVGTQTI  506 Arabidopsis G634 TH: 59-147 2674 SGNRWPREETLALLRIRSDM 100% (G634) thaliana DSTFRDATLKAPLWEHVSRK LLELGYKRSSKKCKEKFENV QKYYKRTKETRGGRIHDGKA YKFFSQLEAL  506 Arabidopsis G634 TH: 187-256 2675 SSRWPKAEILALINLRSGME 100% (G634) thaliana PRYQDNVPKGLLWEEISTSM KRMGYNRNAKRCKEKWENIN KYYKKVKESN  510 Arabidopsis G636 TH: 57-145 2677 GGNRWPRQETLALLKIRSDM  71% (G634) thaliana GIAFRDASVKGPLWEEVSRK MAEHGYIRINAKKCKEKFEN VYKYHKRTKEGRTGKSEGKT YRFFDQLEAL  510 Arabidopsis G636 TH: 405-474 2678 SSRWPKVEIEALIKLRTNLD  72% (G634) thaliana SKYQENGPKGPLWEEISAGM RRLGFNRNSKRCKEKWENIN KYFKKVKESN 2220 Oryza G3917 TH: 194-282 3696 GGNRWPRQETLALLKIRSDM  80% (G634) sativa DAAFRDATLKGPLWEEVSRK LAEEGYRRSAKKCKEKFENV HKYYKRTKESRAGRNDGKTY RFFTQLEAL 2220 Arabidopsis G3917 TH: 508-577 3697 SSRWPKAEVHALIQLRSNLD  72% (G634) thaliana NRYQEAGPKGPLWEEISAGM RRLGYSRSSKRCKEKWENIN KYFKKVKESN

Orthologs and Paralogs

Homologous sequences as described above can comprise orthologous or paralogous sequences. Several different methods are known by those of skill in the art for identifying and defining these functionally homologous sequences. Three general methods for defining orthologs and paralogs are described; an ortholog or paralog, including equivalogs, may be identified by one or more of the methods described below.

As described by Eisen (1998) Genome Res. 8: 163-167, evolutionary information may be used to predict gene function. It is common for groups of genes that are homologous in sequence to have diverse, although usually related, functions. However, in many cases, the identification of homologs is not sufficient to make specific predictions because not all homologs have the same function. Thus, an initial analysis of functional relatedness based on sequence similarity alone may not provide one with a means to determine where similarity ends and functional relatedness begins. Fortunately, it is well known in the art that protein function can be classified using phylogenetic analysis of gene trees combined with the corresponding species. Functional predictions can be greatly improved by focusing on how the genes became similar in sequence (i.e., by evolutionary processes) rather than on the sequence similarity itself (Eisen, supra). In fact, many specific examples exist in which gene function has been shown to correlate well with gene phylogeny (Eisen, supra). Thus, “[t]he first step in making functional predictions is the generation of a phylogenetic tree representing the evolutionary history of the gene of interest and its homologs. Such trees are distinct from clusters and other means of characterizing sequence similarity because they are inferred by techniques that help convert patterns of similarity into evolutionary relationships . . . . After the gene tree is inferred, biologically determined functions of the various homologs are overlaid onto the tree. Finally, the structure of the tree and the relative phylogenetic positions of genes of different functions are used to trace the history of functional changes, which is then used to predict functions of [as yet] uncharacterized genes” (Eisen, supra).

Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and often similar function known as paralogs. A paralog is therefore a similar gene formed by duplication within the same species. Paralogs typically cluster together or in the same clade (a group of similar genes) when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al. (1994); Higgins et al. (1996)). Groups of similar genes can also be identified with pair-wise BLAST analysis (Feng and Doolittle (1987)). For example, a clade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al. (2001)), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al. (1998)). Analysis of groups of similar genes with similar function that fall within one clade can yield sub-sequences that are particular to the clade. These sub-sequences, known as consensus sequences, can not only be used to define the sequences within each clade, but define the functions of these genes; genes within a clade may contain paralogous sequences, or orthologous sequences that share the same function (see also, for example, Mount (2001))

Speculation, the production of new species from a parental species, can also give rise to two or more genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species. Once a phylogenic tree for a gene family of one species has been constructed using a program such as CLUSTAL (Thompson et al. (1994); Higgins et al. (1996)) potential orthologous sequences can be placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Orthologous sequences can also be identified by a reciprocal BLAST strategy. Once an orthologous sequence has been identified, the function of the ortholog can be deduced from the identified function of the reference sequence.

Transcription factor gene sequences are conserved across diverse eukaryotic species lines (Goodrich et al. (1993); Lin et al. (1991); Sadowski et al. (1988)). Plants are no exception to this observation; diverse plant species possess transcription factors that have similar sequences and functions.

Orthologous genes from different organisms have highly conserved functions, and very often essentially identical functions (Lee et al. (2002); Remm et al. (2001)). Paralogous genes, which have diverged through gene duplication, may retain similar functions of the encoded proteins. In such cases, paralogs can be used interchangeably with respect to certain embodiments of the instant invention (for example, transgenic expression of a coding sequence). An example of such highly related paralogs is the CBF family, with three well-defined members in Arabidopsis and at least one ortholog in Brassica napus, all of which control pathways involved in both freezing and drought stress (Gilmour et al. (1998); Jaglo et al. (2001)).

Distinct Arabidopsis transcription factors, including G28 (found in U.S. Pat. No. 6,664,446), G482 (found in US Patent Application 20040045049), G867 (found in US Patent Application 20040098764), and G1073 (found in U.S. Pat. No. 6,717,034), have been shown to confer stress tolerance or increased biomass when the sequences are overexpressed. The polypeptides sequences belong to distinct clades of transcription factor polypeptides that include members from diverse species. In each case, a significant number of clade member sequences derived from both eudicots and monocots have been shown to confer greater biomass or tolerance to stress when the sequences were overexpressed (unpublished data). These references may serve to represent the many studies that demonstrate that conserved transcription factor genes from diverse species are likely to function similarly (i.e., regulate similar target sequences and control the same traits), and that transcription factors may be transformed into diverse species to confer or improve traits.

As shown in Tables 3-33, transcription factors that are phylogenetically related to the transcription factors of the invention may have conserved domains that share at least 38% amino acid sequence identity, and have similar functions.

At the nucleotide level, the sequences of the invention will typically share at least about 30% or 40% nucleotide sequence identity, preferably at least about 50%, about 60%, about 70% or about 80% sequence identity, and more preferably about 85%, about 90%, about 95% or about 97% or more sequence identity to one or more of the listed full-length sequences, or to a listed sequence but excluding or outside of the region(s) encoding a known consensus sequence or consensus DNA-binding site, or outside of the region(s) encoding one or all conserved domains. The degeneracy of the genetic code enables major variations in the nucleotide sequence of a polynucleotide while maintaining the amino acid sequence of the encoded protein.

Percent identity can be determined electronically, e.g., by using the MEGALIGN program (DNASTAR, Inc. Madison, Wis.). The MEGALIGN program can create alignments between two or more sequences according to different methods, for example, the clustal method (see, for example, Higgins and Sharp (1988) The clustal algorithm groups sequences into clusters by examining the distances between all pairs. The clusters are aligned pairwise and then in groups. Other alignment algorithms or programs may be used, including FASTA, BLAST, or ENTREZ, FASTA and BLAST, and which may be used to calculate percent similarity. These are available as a part of the GCG sequence analysis package (University of Wisconsin, Madison, Wis.), and can be used with or without default settings. ENTREZ is available through the National Center for Biotechnology Information. In one embodiment, the percent identity of two sequences can be determined by the GCG program with a gap weight of 1, e.g., each amino acid gap is weighted as if it were a single amino acid or nucleotide mismatch between the two sequences (see U.S. Pat. No. 6,262,333).

Software for performing BLAST analyses is publicly available, e.g., through the National Center for Biotechnology Information (see internet website at www.ncbi.nlm.nih.gov/). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul (1993); Altschul et al. (1990)). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff (1992). Unless otherwise indicated for comparisons of predicted polynucleotides, “sequence identity” refers to the % sequence identity generated from a tblastx using the NCBI version of the algorithm at the default settings using gapped alignments with the filter “off” (see, for example, internet website at www.ncbi.nlm.nih.gov/).

Other techniques for alignment are described by Doolittle (1996). Preferably, an alignment program that permits gaps in the sequence is utilized to align the sequences. The Smith-Waterman is one type of algorithm that permits gaps in sequence alignments (see Shpaer (1997). Also, the GAP program using the Needleman and Wunsch alignment method can be utilized to align sequences. An alternative search strategy uses MPSRCH software, which runs on a MASPAR computer. MPSRCH uses a Smith-Waterman algorithm to score sequences on a massively parallel computer. This approach improves ability to pick up distantly related matches, and is especially tolerant of small gaps and nucleotide sequence errors. Nucleic acid-encoded amino acid sequences can be used to search both protein and DNA databases.

The percentage similarity between two polypeptide sequences, e.g., sequence A and sequence B, is calculated by dividing the length of sequence A, minus the number of gap residues in sequence A, minus the number of gap residues in sequence B, into the sum of the residue matches between sequence A and sequence B, times one hundred. Gaps of low or of no similarity between the two amino acid sequences are not included in determining percentage similarity. Percent identity between polynucleotide sequences can also be counted or calculated by other methods known in the art, e.g., the Jotun Hein method (see, for example, Hein (1990)) Identity between sequences can also be determined by other methods known in the art, e.g., by varying hybridization conditions (see US Patent Application No. 20010010913).

Thus, the invention provides methods for identifying a sequence similar or paralogous or orthologous or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an internet or intranet) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.

In addition, one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to search against a BLOCKS (Bairoch et al. (1997)), PFAM, and other databases which contain previously identified and annotated motifs, sequences and gene functions. Methods that search for primary sequence patterns with secondary structure gap penalties (Smith et al. (1992)) as well as algorithms such as Basic Local Alignment Search Tool (BLAST; Altschul (1993); Altschul et al. (1990)), BLOCKS (Henikoff and Henikoff (1991)), Hidden Markov Models (HMM; Eddy (1996); Sonnhammer et al. (1997)), and the like, can be used to manipulate and analyze polynucleotide and polypeptide sequences encoded by polynucleotides. These databases, algorithms and other methods are well known in the art and are described in Ausubel et al. (1997), and in Meyers (1995).

A further method for identifying or confirming that specific homologous sequences control the same function is by comparison of the transcript profile(s) obtained upon overexpression or knockout of two or more related transcription factors. Since transcript profiles are diagnostic for specific cellular states, one skilled in the art will appreciate that genes that have a highly similar transcript profile (e.g., with greater than 50% regulated transcripts in common, or with greater than 70% regulated transcripts in common, or with greater than 90% regulated transcripts in common) will have highly similar functions. Fowler et al. (2002), have shown that three paralogous AP2 family genes (CBF1, CBF2 and CBF3), each of which is induced upon cold treatment, and each of which can condition improved freezing tolerance, have highly similar transcript profiles. Once a transcription factor has been shown to provide a specific function, its transcript profile becomes a diagnostic tool to determine whether paralogs or orthologs have the same function.

Furthermore, methods using manual alignment of sequences similar or homologous to one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to identify regions of similarity and conserved domains. Such manual methods are well-known of those of skill in the art and can include, for example, comparisons of tertiary structure between a polypeptide sequence encoded by a polynucleotide that comprises a known function and a polypeptide sequence encoded by a polynucleotide sequence that has a function not yet determined. Such examples of tertiary structure may comprise predicted alpha helices, beta-sheets, amphipathic helices, leucine zipper motifs, zinc finger motifs, proline-rich regions, cysteine repeat motifs, and the like.

Orthologs and paralogs of presently disclosed transcription factors may be cloned using compositions provided by the present invention according to methods well known in the art. cDNAs can be cloned using mRNA from a plant cell or tissue that expresses one of the present transcription factors. Appropriate mRNA sources may be identified by interrogating Northern blots with probes designed from the present transcription factor sequences, after which a library is prepared from the mRNA obtained from a positive cell or tissue. Transcription factor-encoding cDNA is then isolated using, for example, PCR, using primers designed from a presently disclosed transcription factor gene sequence, or by probing with a partial or complete cDNA or with one or more sets of degenerate probes based on the disclosed sequences. The cDNA library may be used to transform plant cells. Expression of the cDNAs of interest is detected using, for example, microarrays, Northern blots, quantitative PCR, or any other technique for monitoring changes in expression. Genomic clones may be isolated using similar techniques to those.

Examples of orthologs of the Arabidopsis polypeptide sequences and their functionally similar orthologs are listed in the Sequence Listing. In addition to the sequences in the Sequence Listing, the invention encompasses isolated nucleotide sequences that are phylogenetically and structurally similar to sequences listed in the Sequence Listing) and can function in a plant by increasing biomass, disease resistance and/or and abiotic stress tolerance when ectopically expressed in a plant. These polypeptide sequences represent transcription factors that show significant sequence similarity the polypeptides of the Sequence Listing particularly in their respective conserved domains, as identified in Tables 3-33.

Since a significant number of these sequences are phylogenetically and sequentially related to each other and have been shown to increase a plant's biomass, disease resistance and/or abiotic stress tolerance, one skilled in the art would predict that other similar, phylogenetically related sequences falling within the present clades of transcription factors would also perform similar functions when ectopically expressed.

Identifying Polynucleotides or Nucleic Acids by Hybridization

Polynucleotides homologous to the sequences illustrated in the Sequence Listing and tables can be identified, e.g., by hybridization to each other under stringent or under highly stringent conditions. Single stranded polynucleotides hybridize when they associate based on a variety of well characterized physical-chemical forces, such as hydrogen bonding, solvent exclusion, base stacking and the like. The stringency of a hybridization reflects the degree of sequence identity of the nucleic acids involved, such that the higher the stringency, the more similar are the two polynucleotide strands. Stringency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc. present in both the hybridization and wash solutions and incubations (and number thereof), as described in more detail in the references cited below (e.g., Sambrook et al. (1989); Berger and Kimmel (1987); and Anderson and Young (1985)).

Encompassed by the invention are polynucleotide sequences that are capable of hybridizing to the claimed polynucleotide sequences, including any of the transcription factor polynucleotides within the Sequence Listing, and fragments thereof under various conditions of stringency (see, for example, Wahl and Berger (1987); and Kimmel (1987)). In addition to the nucleotide sequences listed in the Sequence Listing, full length cDNA, orthologs, and paralogs of the present nucleotide sequences may be identified and isolated using well-known methods. The cDNA libraries, orthologs, and paralogs of the present nucleotide sequences may be screened using hybridization methods to determine their utility as hybridization target or amplification probes.

With regard to hybridization, conditions that are highly stringent, and means for achieving them, are well known in the art. See, for example, Sambrook et al. (1989); Berger (1987), pages 467-469; and Anderson and Young (1985).

Stability of DNA duplexes is affected by such factors as base composition, length, and degree of base pair mismatch. Hybridization conditions may be adjusted to allow DNAs of different sequence relatedness to hybridize. The melting temperature (T_(m)) is defined as the temperature when 50% of the duplex molecules have dissociated into their constituent single strands. The melting temperature of a perfectly matched duplex, where the hybridization buffer contains formamide as a denaturing agent, may be estimated by the following equations:

(I) DNA-DNA:

T_(m)(° C.)=81.5+16.6(log [Na+])+0.41(% G+C)−0.62(% formamide)−500/L

(II) DNA-RNA:

T_(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(% G+C)²-0.5(% formamide)−820/L

(III) RNA-RNA:

T_(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(% G+C)²−0.35(% formamide)−820/L

where L is the length of the duplex formed, [Na+] is the molar concentration of the sodium ion in the hybridization or washing solution, and % G+C is the percentage of (guanine+cytosine) bases in the hybrid. For imperfectly matched hybrids, approximately 1° C. is required to reduce the melting temperature for each 1% mismatch.

Hybridization experiments are generally conducted in a buffer of pH between 6.8 to 7.4, although the rate of hybridization is nearly independent of pH at ionic strengths likely to be used in the hybridization buffer (Anderson and Young (1985)). In addition, one or more of the following may be used to reduce non-specific hybridization: sonicated salmon sperm DNA or another non-complementary DNA, bovine serum albumin, sodium pyrophosphate, sodium dodecylsulfate (SDS), polyvinyl-pyrrolidone, ficoll and Denhardt's solution. Dextran sulfate and polyethylene glycol 6000 act to exclude DNA from solution, thus raising the effective probe DNA concentration and the hybridization signal within a given unit of time. In some instances, conditions of even greater stringency may be desirable or required to reduce non-specific and/or background hybridization. These conditions may be created with the use of higher temperature, lower ionic strength and higher concentration of a denaturing agent such as formamide.

Stringency conditions can be adjusted to screen for moderately similar fragments such as homologous sequences from distantly related organisms, or to highly similar fragments such as genes that duplicate functional enzymes from closely related organisms. The stringency can be adjusted either during the hybridization step or in the post-hybridization washes. Salt concentration, formamide concentration, hybridization temperature and probe lengths are variables that can be used to alter stringency (as described by the formula above). As a general guidelines high stringency is typically performed at T_(m)-5° C. to T_(m)-20° C., moderate stringency at T_(m)-20° C. to T_(m)-35° C. and low stringency at T_(m)-35° C. to T_(m)-50° C. for duplex >150 base pairs. Hybridization may be performed at low to moderate stringency (25-50° C. below T_(m)), followed by post-hybridization washes at increasing stringencies. Maximum rates of hybridization in solution are determined empirically to occur at T_(m)-25° C. for DNA-DNA duplex and T_(m)-15° C. for RNA-DNA duplex. Optionally, the degree of dissociation may be assessed after each wash step to determine the need for subsequent, higher stringency wash steps.

High stringency conditions may be used to select for nucleic acid sequences with high degrees of identity to the disclosed sequences. An example of stringent hybridization conditions obtained in a filter-based method such as a Southern or Northern blot for hybridization of complementary nucleic acids that have more than 100 complementary residues is about 5° C. to 20° C. lower than the thermal melting point (T_(m)) for the specific sequence at a defined ionic strength and pH. Conditions used for hybridization may include about 0.02 M to about 0.15 M sodium chloride, about 0.5% to about 5% casein, about 0.02% SDS or about 0.1% N-laurylsarcosine, about 0.001 M to about 0.03 M sodium citrate, at hybridization temperatures between about 50° C. and about 70° C. More preferably, high stringency conditions are about 0.02 M sodium chloride, about 0.5% casein, about 0.02% SDS, about 0.001 M sodium citrate, at a temperature of about 50° C. Nucleic acid molecules that hybridize under stringent conditions will typically hybridize to a probe based on either the entire DNA molecule or selected portions, e.g., to a unique subsequence, of the DNA.

Stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate. Increasingly stringent conditions may be obtained with less than about 500 mM NaCl and 50 mM trisodium citrate, to even greater stringency with less than about 250 mM NaCl and 25 mM trisodium citrate. Low stringency hybridization can be obtained in the absence of organic solvent, e.g., formamide, whereas high stringency hybridization may be obtained in the presence of at least about 35% formamide, and more preferably at least about 50% formamide. Stringent temperature conditions will ordinarily include temperatures of at least about 30° C., more preferably of at least about 37° C., and most preferably of at least about 42° C. with formamide present. Varying additional parameters, such as hybridization time, the concentration of detergent, e.g., sodium dodecyl sulfate (SDS) and ionic strength, are well known to those skilled in the art. Various levels of stringency are accomplished by combining these various conditions as needed.

The washing steps that follow hybridization may also vary in stringency; the post-hybridization wash steps primarily determine hybridization specificity, with the most critical factors being temperature and the ionic strength of the final wash solution. Wash stringency can be increased by decreasing salt concentration or by increasing temperature. Stringent salt concentration for the wash steps will preferably be less than about 30 mM NaCl and 3 mM trisodium citrate, and most preferably less than about 15 mM NaCl and 1.5 mM trisodium citrate.

Thus, hybridization and wash conditions that may be used to bind and remove polynucleotides with less than the desired homology to the nucleic acid sequences or their complements that encode the present transcription factors include, for example:

0.2× to 2×SSC and 0.1% SDS at 50° C., 55° C., 60° C., 65° C., or 50° C. to 65° C.;

6×SSC at 65° C.;

50% formamide, 4×SSC at 42° C.; or 0.5×, 1×, or 1.5×SSC, 0.1% SDS at 50° C., 55° C., 60° C., or 65° C.;

with, for example, two wash steps of 10-30 minutes each. Useful variations on these conditions will be readily apparent to those skilled in the art. A formula for “SSC, 20×” may be found, for example, in Ausubel et al., 1997, in Appendix A1.

A person of skill in the art would not expect substantial variation among polynucleotide species encompassed within the scope of the present invention because the highly stringent conditions set forth in the above formulae yield structurally similar polynucleotides.

If desired, one may employ wash steps of even greater stringency, including about 0.2×SSC, 0.1% SDS at 65° C. and washing twice, each wash step being about 30 minutes, or about 0.1×SSC, 0.1% SDS at 650 C and washing twice for 30 minutes. The temperature for the wash solutions will ordinarily be at least about 25° C., and for greater stringency at least about 42° C. Hybridization stringency may be increased further by using the same conditions as in the hybridization steps, with the wash temperature raised about 3° C. to about 5° C., and stringency may be increased even further by using the same conditions except the wash temperature is raised about 6° C. to about 9° C. For identification of less closely related homologs, wash steps may be performed at a lower temperature, e.g., 50° C.

An example of a low stringency wash step employs a solution and conditions of at least 25° C. in 30 mM NaCl, 3 mM trisodium citrate, and 0.1% SDS over 30 minutes. Greater stringency may be obtained at 42° C. in 15 mM NaCl, with 1.5 mM trisodium citrate, and 0.1% SDS over 30 minutes. Even higher stringency wash conditions are obtained at 65° C.-68° C. in a solution of 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. Wash procedures will generally employ at least two final wash steps. Additional variations on these conditions will be readily apparent to those skilled in the art (see, for example, US Patent Application No. 20010010913).

Stringency conditions can be selected such that an oligonucleotide that is perfectly complementary to the coding oligonucleotide hybridizes to the coding oligonucleotide with at least about a 5-10× higher signal to noise ratio than the ratio for hybridization of the perfectly complementary oligonucleotide to a nucleic acid encoding a transcription factor known as of the filing date of the application. It may be desirable to select conditions for a particular assay such that a higher signal to noise ratio, that is, about 15× or more, is obtained. Accordingly, a subject nucleic acid will hybridize to a unique coding oligonucleotide with at least a 2× or greater signal to noise ratio as compared to hybridization of the coding oligonucleotide to a nucleic acid encoding known polypeptide. The particular signal will depend on the label used in the relevant assay, e.g., a fluorescent label, a calorimetric label, a radioactive label, or the like. Labeled hybridization or PCR probes for detecting related polynucleotide sequences may be produced by oligolabeling, nick translation, end-labeling, or PCR amplification using a labeled nucleotide.

Encompassed by the invention are polynucleotide sequences that are capable of hybridizing to the claimed polynucleotide sequences, including any of the transcription factor polynucleotides within the Sequence Listing, and fragments thereof under various conditions of stringency (see, for example, Wahl and Berger (1987), pages 399-407; and Kimmel (1987)). In addition to the nucleotide sequences in the Sequence Listing, full length cDNA, orthologs, and paralogs of the present nucleotide sequences may be identified and isolated using well-known methods. The cDNA libraries, orthologs, and paralogs of the present nucleotide sequences may be screened using hybridization methods to determine their utility as hybridization target or amplification probes.

EXAMPLES

It is to be understood that this invention is not limited to the particular devices, machines, materials and methods described. Although particular embodiments are described, equivalent embodiments may be used to practice the invention.

The invention, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention and are not intended to limit the invention. It will be recognized by one of skill in the art that a transcription factor that is associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait which was not predicted by the first trait.

Example 1 Project Types

A variety of constructs were used to modulate the activity of lead transcription factors, and to test the activity of orthologs and paralogs in transgenic plant material. This platform provided the material for all subsequent analysis.

Transgenic lines from each particular transformation “project” were examined for morphological and physiological phenotypes. An individual project was defined as the analysis of lines for a particular construct or knockout (for example this might be 35S lines for a lead gene, 35S lines for a paralog or ortholog, lines for an RNAi construct, lines for a GAL4 fusion construct, lines in which expression is driven from a particular tissue specific promoter, etc.) In the current lead advancement program, four main areas of analysis were pursued, spanning a variety of different project types (e.g., promoter-gene combinations).

(1) Overexpression/Tissue-Specific/Conditional Expression

The promoters used in our experiments were selected in order to provide for a range of different expression patterns. Details of promoters being used are provided in Example II.

Expression of a given TF from a particular promoter was achieved either by a direct-promoter fusion construct in which that TF was cloned directly behind the promoter of interest or by a two component system. Details of transformation vectors used in these studies are shown in the Vector and Cloning Information (Example III). A list of all constructs used in these analyses (PIDs), including compilations of the sequences of promoter fragments and the expressed transgene sequences within the PIDs, are provided in the Sequence Listing.

The Two-Component Expression System

For the two-component system, two separate constructs were used: Promoter::LexA-GAL4TA and opLexA::TF. The first of these (Promoter::LexA-GAL4TA) comprised a desired promoter cloned in front of a LexA DNA binding domain fused to a GAL4 activation domain. The construct vector backbone (pMEN48, also known as P5375) also carried a kanamycin resistance marker, along with an opLexA::GFP (green fluorescent protein) reporter. Transgenic lines were obtained containing this first component, and a line was selected that shows reproducible expression of the reporter gene in the desired pattern through a number of generations. A homozygous population was established for that line, and the population was supertransformed with the second construct (opLexA::TF) carrying the TF of interest cloned behind a LexA operator site. This second construct vector backbone (pMEN53, also known as P5381) also contained a sulfonamide resistance marker.

Each of the above methods offers a number of pros and cons. A direct fusion approach allows for much simpler genetic analysis if a given promoter-TF line is to be crossed into different genetic backgrounds at a later date. The two-component method, on the other hand, potentially allows for stronger expression to be obtained via an amplification of transcription. Additionally, a range of two-component constructs were available at the start of the Lead Advancement program which had been built using funding from an Advanced Technology Program (ATP) grant.

In general, Arabidopsis TFs from different study groups were expressed from a range of different promoters, often with a two component method. Arabidopsis paralogs were also generally analyzed by the two-component method, but were typically analyzed using the only 35S promoter. However, an alternative promoter was sometimes used for paralogs when there was a specific indication that a different promoter might afford a more useful approach (such as when use of the 35S promoter was known to generate deleterious effects). Putative orthologs from other species were usually analyzed by overexpression from a 35S CaMV promoter via a direct promoter-fusion construct. The vector backbone for most of the direct promoter-fusion overexpression constructs was pMEN65, but pMEN1963 and pMEN20 were sometimes used.

(2) Knock-Out/Knock-Down

Where available, T-DNA insertion lines from either the public or the in-house collections were analyzed.

In cases where a T-DNA insertion line was unavailable, an RNA interference (RNAi) strategy was sometimes used. At the outset of the program, the system was tested with two well-characterized genes [LEAFY (Weigel et al., 1992) and CONSTANS (Putterill et al., 1995)] that gave clear morphological phenotypes when mutated. In each case, RNAi lines were obtained that exhibited characters seen in the null mutants.

(3) Protein Modifications

Deletion Variants

Truncated versions or fragments of the leads were sometimes overexpressed to test hypotheses regarding particular parts of the proteins. Such an approach can result in dominant negative alleles.

Point Mutation and Domain Swap Variants

In order to assess the role of particular conserved residues or domains, mutated versions of lead proteins with substitutions at those residues were overexpressed. In some cases, we also overexpressed chimeric variants of the transcription factor in which one or domains have been exchanged with another transcription factor.

(4) Analytical Tools for Pathway Analysis

Promoter-Reporter Constructs

Promoters were primarily cloned in front of a GUS reporter system. These constructs were used to identify putative upstream transcriptional activators via a transient assay. In most cases approximately 2 kb of the sequence immediately 5′ to the ATG of the gene was included in the construct.

In addition to being used in transient assays, the promoter-reporter constructs were transformed into Arabidopsis. The lines were then used to characterize the expression patterns of the lead genes in planta over a variety of tissue types and stress conditions. As well as GUS, a number of fluorescent reporter proteins were used in Promoter-reporter constructs including GFP, YFP (yellow fluorescent protein), CFP (cyan fluorescent protein) and anchored variants of YFP such as YFP-LTI6.

Protein Fusions to Fluorescent Tags

To examine sub-cellular localization of TFs, translational fusions to fluorescent markers such as GFP, CFP, and YFP were used.

Dexamethasone Inducible Lines

Glucocorticoid receptor fusions at the N and C termini of the primary TFs were constructed to allow the identification of their immediate/early targets during array-based studies. We also produced dexamethasone inducible lines via a two-component approach.

TABLE 34 Definitions of particular project types Project type Definition Direct promoter- A full-length wild-type version of a gene was directly fused to a promoter that drove its fusion (DPF) expression in transgenic plants. Such a promoter could be the native promoter or that gene, 35S, or a promoter that drove tissue specific or conditional expression. 2-components- A full-length wild-type version of a gene was expressed via the 2 component, supTfn (TCST) promoter::LexA-GAL4; opLexA::TF system. In this case, a stable transgenic line was first established containing one of the components and was later supertransformed with the second component. splice_variant_* A splice variant of a gene was directly fused to a promoter that drove its expression in transgenic plants. Such a promoter was the native promoter or that gene, 35S, or a promoter that drove tissue specific or conditional expression. Direct GR-fusion C- A construct contained a TF with a direct C-terminal fusion to a glucocorticoid receptor. term Direct GR-fusion N- A construct contained a TF with a direct N-terminal fusion to a glucocorticoid receptor. term Direct GR-fusion A construct contains a TF with a direct C-terminal fusion to a glucocorticoid receptor in HA C-term combination with an HA (hemagglutinin) epitope tag in the conformation: TF-GR-HA Direct GR-fusion A construct contained a TF with a direct N-terminal fusion to a glucocorticoid receptor in HA N-term combination with an HA (hemagglutinin) epitope tag in the conformation: GR-TF-HA GAL4 C-term A TF with a C-terminal fusion to a GAL4 activation domain was overexpressed. GAL4 N-term A TF with an N-terminal fusion to a GAL4 activation domain was overexpressed. TF dominant A truncated variant or fragment of a TF was (over)expressed, often with the aim of producing negative deletion a dominant negative phenotype. Usually the truncated version comprised the DNA binding domain. TF dom neg deln A truncated variant or fragment of a TF was (over)expressed, often with the aim of producing 2ndry domain a dominant negative phenotype. In this case, the truncated version contained a conserved secondary domain (rather than the main DNA binding domain) or a secondary DNA binding domain alone, in the case when a TF had two potential binding domains (e.g. B3 &AP2). deletion_* A variant of a TF was (over)expressed in which one or more regions had been deleted. site-directed A form of the protein was overexpressed which had had one or more residues changed by site mutation_* directed mutagenesis. domain swap_* A form of the protein was overexpressed in which a particular fragment had been substituted with a region from another protein. KO Describes a line that harbored a mutation in an Arabidopsis TF at its endogenous locus. In most cases this was caused by a T-DNA insertion. RNAi (clade) An RNAi construct designed to knock-down a clade of related genes. RNAi (GS) An RNAi construct designed to knock-down a specific gene. Promoter-reporter A construct used to determine the expression pattern of a gene, or in transient assay experiments. This was typically a promoter-GUS or promoter-GFP (or a derivative of GFP) fusion. Protein-GFP-C- Overexpression of a translational fusion in which the TF had GFP fused to the C-terminus. fusion Protein-YFP-C- Overexpression of a translational fusion in which the TF had YFP fused to the C-terminus. fusion Protein-CFP-C- Overexpression of a translational fusion in which the TF had CFP fused to the C-terminus. fusion 2-components- Overexpression of a translational fusion in which the TF had a TAP tag (Tandem affinity supTfn-TAP-C-term purification epitope, see Rigaut et al., 1999 and Rohila et al., 2004) fused to the C-terminus. This fusion was expressed via the two-component system: promoter::LexA- GAL4; opLexA::TF-TAP. In this case, a stable transgenic line was first established containing the promoter component and was later supertransformed with the TF-TAP component). 2-components- Overexpression of a translational fusion in which the TF had an HA (hemagglutinin) epitope supTfh-HA-C-term tag fused to the C-terminus. This fusion was expressed via the two-component system: promoter::LexA-GAL4; opLexA::TF-HA. In this case, a stable transgenic line was first established containing the promoter component and was later supertransformed with the TF- HA component). 2-components- Overexpression of a translational fusion in which the TF had an HA (hemagglutinin) epitope supTfn-HA-N-term tag fused to the N-terminus. This fusion was expressed via the two-component system: promoter::LexA-GAL4; opLexA::HA-TF. In this case, a stable transgenic line was first established containing the promoter component and was later supertransformed with the HA- TF component). Double A transgenic line harboring two different overexpression constructs, created by a genetic Overexpression crossing approach. (Double OE) Cross Triple A transgenic line harboring three different overexpression constructs, created by a genetic Overexpression crossing approach. (Triple OE) Cross *designates any numeric value

Example II Promoter Analysis

A major component of the program was to determine the effects of ectopic expression of transcription factors in a variety of different tissue types, and in response to the onset of stress conditions. Primarily this was achieved by using a panel of different promoters via a two-component system.

Component 1: promoter driver lines (Promoter::LexA/GAL4). In each case, the first component (Promoter::LexA/GAL4) comprised a LexA DNA binding domain fused to a GAL4 activation domain, cloned behind the desired promoter. These constructs were contained within vector backbone pMEN48 (Example III) which also carried a kanamycin resistance marker, along with an opLexA::GFP reporter. The GFP was EGFP, an variant available from Clontech (Mountain View, Calif.) with enhanced signal. EGFP is soluble in the cytoplasm. Transgenic “driver lines” were first obtained containing the Promoter::LexA/GAL4 component. For each promoter driver, a line was selected which showed reproducible expression of the GFP reporter gene in the desired pattern, through a number of generations. We also tested the plants in our standard plate based physiology assays to verify that the tissue specific pattern was not substantially altered by stress conditions. A homozygous population was then established for that line.

Component 2: TF construct (opLexA::TF). Having established a promoter panel, it was possible to overexpress any transcription factor in the precise expression pattern conferred by the driver lines, by super-transforming or crossing in a second construct (opLexA::TF) carrying the TF of interest cloned behind a LexA operator site. In each case this second construct carried a sulfonamide selectable marker and was contained within vector backbone pMEN53 (see Example III).

Arabidopsis promoter driver lines are shown in Table 35.

TABLE 35 Expression patterns conferred by promoters used for one (i.e., in some 35S overexpressing lines) and two-component studies. Promoter Expression pattern conferred Reference 35S Constitutive, high levels of expression in all Odell et al. (1985) throughout the plant and fruit SUC2 Vascular/Phloem Truernit and Sauer (1995) ARSK1 Root Hwang and Goodman (1995) CUT1 Shoot epidermal/guard cell enhanced Kunst et al. (2000) RBCS3 Photosynthetic tissue; expression Wanner and Gruissem (1991) predominately in highly photosynthetic vegetative tissues. Fruit expression predominately in the pericarp RD29A* Drought/Cold/ABA inducible Yamaguchi-Shinozaki and Shinozaki (1993) LTP1 Shoot epidermal/trichome enhanced; in Thoma et al. (1994) vegetative tissues, expression is predominately in the epidermis. Low levels of expression are also evident in vascular tissue. In the fruit, expression is strongest in the pith-like columella/placental tissue RSI1 Root meristem and root vascular; expression Taylor and Scheuring (1994) generally limited to roots. Also expressed in the vascular tissues of the fruit. AP1 Flower primordia/flower; light expression in Hempel et al. (1997); Mandel et al. (1992) leaves increases with maturation. Highest expression in flower primordia and flower organs. In fruits, predominately in pith-like columella/placental tissue STM Expressed in meristematic tissues, including Long and Barton (2000); Long et al. (1996) apical meristems, cambium. Low levels of expression also in some differentiating tissues. In fruit, most strongly expressed in vascular tissues and endosperm. AS1 Primordia and young organs; expressed Byrne et al. (2000) predominately in differentiating tissues. In fruit, most strongly expressed in vascular tissues and in endosperm PG Phytoene desaturase; high expression Nicholass et al.(1995) throughout the fruit, comparable to 35S. Montgomery et al. (1993) Strongest late in fruit development PD Phytoene desaturase; moderate expression in Corona et al. (1996) fruit tissues CRU Cruciferin 1; expressed at low levels in fruit Breen and Crouch (1992) vascular tissue and columella. Seed and Sjodahl et al. (1995) endosperm expression Notes: *Two different RD29A promoter lines, lines 2 and 5, were used. Line 2 has a higher level of background expression than line 5. Expression from the line 2 promoter was expected to produce constitutive moderate basal transcript levels of any gene controlled by it, and to generate an increase in levels following the onset of stress. In contrast, line 5 was expected to produce lower basal levels and a somewhat sharper up-regulation of any gene under its control, following the onset ofstress. Although RD29A exhibits up-regulation in response to cold and drought in mature tissues, this promoter produces relatively high levels of expression in embryos and young seedlings.

Validation of the Promoter-driver line patterns. To demonstrate that each of the promoter driver lines could generate the desired expression pattern of a second component target at an independent locus arranged in trans, crosses were made to an opLexA::GUS line. Typically, it was confirmed that the progeny exhibited GUS activity in an equivalent region to the GFP seen in the parental promoter driver line. However, GFP can move from cell-to-cell early in development and in meristematic tissues, and hence patterns of GFP in these tissues do not strictly report gene expression.

It was clear that the 35S promoter induces much higher levels of expression compared to the other promoters presently in use.

Example III Vector and Cloning Information Vector and Cloning Information: Expression Vectors.

A list of nucleic acid constructs (PIDs) included in this application, indicating the promoter fragment that was used to drive the transgene, along with the cloning vector backbone, is provided in the Sequence listing as SEQ ID NOs 3792-5086 and 5102-5106.

Target sequences were selected to be 100 bp long or longer. For constructs designed against a clade rather than a single gene, the target sequences had at least 85% identity to all clade members. Where it is not possible to identify a single 100 bp sequence with 85% identity to all clade members, hybrid fragments composed of two shorter sequences were used.

Cloning Methods.

Arabidopsis transcription factor clones were created in one of three ways: isolation from a library, amplification from cDNA, or amplification from genomic DNA. The ends of the Arabidopsis transcription factor coding sequences were generally confirmed by RACE PCR or by comparison with public cDNA sequences before cloning.

Clones of transcription factor orthologs from rice, maize, and soybean were all made by amplification from cDNA. The ends of the coding sequences were predicted based on homology to Arabidopsis or by comparison to public and proprietary cDNA sequences; RACE PCR was not done to confirm the ends of the coding sequences. For cDNA amplification, KOD Hot Start DNA Polymerase (Novagen, Madison, Wis.) was used in combination with 1M betaine and 3% DMSO. This protocol was found to be successful in amplifying cDNA from GC-rich species such as rice and corn, along with some non-GC-rich species such as soybean and tomato, where traditional PCR protocols failed. Primers were designed using at least 30 bases specific to the target sequence, and were designed close to, or overlapping, the start and stop codons of the predicted coding sequence.

Clones were fully sequenced. In the case of rice, high-quality public genomic sequences were available for comparison, and clones with sequence changes that result in changes in amino acid sequence of the encoded protein were rejected. For corn and soy, however, it was often unclear whether sequence differences represent an error or polymorphism in the source sequence or a PCR error in the clone. Therefore, in the cases where the sequence of the clone we obtained differed from the source sequence, a second clone was created from an independent PCR reaction. If the sequences of the two clones agreed, then the clone was accepted as a legitimate sequence variant.

Transformation. Agrobacterium strain ABI was used for all plant transformations. This strain is chloramphenicol, kanamycin and gentamicin resistant.

Example IV Transformation

Transformation of Arabidopsis was performed by an Agrobacterium-mediated protocol based on the method of Bechtold and Pelletier (1998). Unless otherwise specified, all experimental work was done using the Columbia ecotype.

Plant preparation. Arabidopsis seeds were sown on mesh covered pots. The seedlings were thinned so that 6-10 evenly spaced plants remained on each pot 10 days after planting. The primary bolts were cut off a week before transformation to break apical dominance and encourage auxiliary shoots to form. Transformation was typically performed at 4-5 weeks after sowing.

Bacterial culture preparation. Agrobacterium stocks were inoculated from single colony plates or from glycerol stocks and grown with the appropriate antibiotics and grown until saturation. On the morning of transformation, the saturated cultures were centrifuged and bacterial pellets were re-suspended in Infiltration Media (0.5×MS, 1× B5 Vitamins, 5% sucrose, 1 mg/ml benzylaminopurine riboside, 200 μl/L Silwet L77) until an A600 reading of 0.8 is reached.

Transformation and seed harvest. The Agrobacterium solution was poured into dipping containers. All flower buds and rosette leaves of the plants were immersed in this solution for 30 seconds. The plants were laid on their side and wrapped to keep the humidity high. The plants were kept this way overnight at 4° C. and then the pots were turned upright, unwrapped, and moved to the growth racks.

The plants were maintained on the growth rack under 24-hour light until seeds were ready to be harvested. Seeds were harvested when 80% of the siliques of the transformed plants were ripe (approximately 5 weeks after the initial transformation). This seed was deemed T0 seed, since it was obtained from the T0 generation, and was later plated on selection plates (either kanamycin or sulfonamide, see Example VI). Resistant plants that were identified on such selection plates comprised the T1 generation.

Example V Morphology

Arabidopsis is used as a model plant for the study of plant growth and development. In addition to providing ornamental utility, altered morphological or developmental features may affect stress tolerance and ultimately plant quality or yield. For example, alterations to appendages such as hairs and trichomes, stomata, and the deposition of waxes may enhance a plant's ability to take up nutrients or resist disease or pathogens. Dark color may also contribute to oxidative stress tolerance or enhanced photosynthetic capacity, which in turn could result in yield increases.

Thus, morphological analysis was performed to determine whether changes in transcription factor levels affect plant growth and development. This was primarily carried out on the T1 generation, when at least 10-20 independent lines were examined. However, in cases where a phenotype required confirmation or detailed characterization, plants from subsequent generations were also analyzed.

Primary transformants were typically selected on MS medium with 0.3% sucrose and 50 mg/l kanamycin. T2 and later generation plants were selected in the same manner, except that kanamycin was used at 35 mg/l. In cases where lines carry a sulfonamide marker (as in all lines generated by super-transformation), seeds were selected on MS medium with 0.3% sucrose and 1.5 mg/l sulfonamide. KO lines were usually germinated on plates without a selection. Seeds were cold-treated (stratified) on plates for 3 days in the dark (in order to increase germination efficiency) prior to transfer to growth cabinets. Initially, plates were incubated at 22° C. under a light intensity of approximately 100 microEinsteins for 7 days. At this stage, transformants were green, possessed the first two true leaves, and were easily distinguished from bleached kanamycin or sulfonamide-susceptible seedlings. Resistant seedlings were then transferred onto soil (Sunshine potting mix). Following transfer to soil, trays of seedlings were covered with plastic lids for 2-3 days to maintain humidity while they became established. Plants were grown on soil under fluorescent light at an intensity of 70-95 microEinsteins and a temperature of 18-23° C. Light conditions consisted of a 24-hour photoperiod unless otherwise stated. In instances where alterations in flowering time was apparent, flowering was typically re-examined under both 12-hour and 24-hour light to assess whether the phenotype was photoperiod dependent. Under our 24-hour light growth conditions, the typical generation time (seed to seed) was approximately 14 weeks.

Because many aspects of Arabidopsis development are dependent on localized environmental conditions, in all cases plants were evaluated in comparison to controls in the same flat. As noted below, controls for transgenic lines were wild-type plants, plants overexpressing CBF4, or transgenic plants harboring an empty transformation vector selected on kanamycin or sulfonamide. Careful examination was made at the following stages: seedling (1 week), rosette (2-3 weeks), flowering (4-7 weeks), and late seed set (8-12 weeks). Seed was also inspected. Seedling morphology was assessed on selection plates. At all other stages, plants were macroscopically evaluated while growing on soil. All significant differences (including alterations in growth rate, size, leaf and flower morphology, coloration and flowering time) were recorded, but routine measurements were not be taken if no differences were apparent. In certain cases, stem sections were stained to reveal lignin distribution. In these instances, hand-sectioned stems were mounted in phloroglucinol saturated 2M HCl (which stains lignin pink) and viewed immediately under a dissection microscope.

Note that for a given project (gene-promoter combination, GAL4 fusion lines, RNAi lines etc.), ten lines were typically examined in subsequent plate based physiology assays.

Example VI Physiology Experimental Methods

Plate Assays. Twelve different plate-based physiological assays (shown below), representing a variety of drought-stress related conditions, were used as a pre-screen to identify top performing lines from each project (i.e. lines from transformation with a particular construct), that may be tested in subsequent soil based assays. Typically, ten lines were subjected to plate assays, from which the best three lines were selected for subsequent soil based assays. However, in projects where significant stress tolerance was not obtained in plate based assays, lines were not submitted for soil assays.

In addition, transgenic lines were subjected to nutrient limitation studies. A nutrient limitation assay was intended to find genes that allow more plant growth upon deprivation of nitrogen. Nitrogen is a major nutrient affecting plant growth and development that ultimately impacts yield and stress tolerance. These assays monitor primarily root but also rosette growth on nitrogen deficient media. In all higher plants, inorganic nitrogen is first assimilated into glutamate, glutamine, aspartate and asparagine, the four amino acids used to transport assimilated nitrogen from sources (e.g. leaves) to sinks (e.g. developing seeds). This process is regulated by light, as well as by C/N metabolic status of the plant. We used a C/N sensing assay to look for alterations in the mechanisms plants use to sense internal levels of carbon and nitrogen metabolites which could activate signal transduction cascades that regulate the transcription of N-assimilatory genes. To determine whether these mechanisms are altered, we exploited the observation that wild-type plants grown on media containing high levels of sucrose (3%) without a nitrogen source accumulate high levels of anthocyanins. This sucrose induced anthocyanin accumulation can be relieved by the addition of either inorganic or organic nitrogen. We used glutamine as a nitrogen source since it also serves as a compound used to transport N in plants.

G1792 and N

The performance of two G1792-overexpressing lines, G1792-311-9 and G1792-312-8, was examined under limited nitrogen growth conditions. Plants were grown in pots filled with fritted clay, sub-irrigated every two hours with a hydroponic growth solution containing 0.1 mM ammonium nitrate as the sole nitrogen source. These conditions represent nitrogen-limited conditions for Arabidopsis growth. Plants were harvested at the rosette stage after 7 weeks of growth under 10 hour light. Chlorophyll content was measured with a SPAD meter, fresh weight was determined, and percent total nitrogen content was determined by dry combustion (Micro-Dumas combustion analysis). As shown in Table 36 provided below, the two G1792 lines were found to have higher chlorophyll content and total nitrogen concentration. One line produced significantly less biomass than controls.

Germination assays. NaCl (150 mM), mannitol (300 mM), glucose (5%), sucrose (9.4%), PEG (10%, with Phytogel as gelling agent), ABA (0.3 μM), Heat (32° C.), Cold (8° C.), −N is basal media minus nitrogen plus 3% sucrose and −N/+Gln is basal media minus nitrogen plus 3% sucrose and 1 mM glutamine. In addition to being stresses in their own right, salt, mannitol, heat, PEG and high sugar concentrations (e.g., 9.4% sucrose, 300 mM mannitol, 5% glucose), may contribute to hyperosmotic stress in plants and may also be used to assess tolerance to water deficit.

Growth assays. Growth assays consisted of water deficit assays, including severe dehydration assays such as desiccation (plate-based drought assays), or heat (32° C. for 5 days followed by recovery at 22° C.), chilling (8° C.), root development (visual assessment of lateral and primary roots, root hairs and overall growth). For the nitrogen limitation assay, all components of MS medium remained constant except nitrogen was reduced to 20 mg/L of NH₄NO₃. Note that 80% MS had 1.32 g/L NH₄NO₃ and 1.52 g/L KNO₃.

Unless otherwise stated, all experiments were performed with the Arabidopsis thaliana ecotype Columbia (col-0). Assays were usually performed on non-selected segregating T2 populations (in order to avoid the extra stress of selection). Control plants for assays on lines containing direct promoter-fusion constructs were Col-0 plants transformed an empty transformation vector (pMEN65). Controls for 2-component lines (generated by supertransformation) were the background promoter-driver lines (i.e. promoter::LexA-GAL4TA lines), into which the supertransformations were initially performed.

All assays were performed in tissue culture. Growing the plants under controlled temperature and humidity on sterile medium produced uniform plant material that had not been exposed to additional stresses (such as water stress) which could cause variability in the results obtained. All assays were designed to detect plants that were more tolerant or less tolerant to the particular stress condition and were developed with reference to the following publications: Jang et al. (1997), Smeekens (1998), Liu and Zhu (1997), Saleki et al. (1993), Wu et al. (1996), Zhu et al. (1998), Alia et al. (1998), Xin and Browse, (1998), Leon-Kloosterziel et al. (1996). Where possible, assay conditions were originally tested in a blind experiment with controls that had phenotypes related to the condition tested.

Procedures

Prior to plating, seed for all experiments were surface sterilized in the following manner: (1) 5 minute incubation with mixing in 70% ethanol, (2) 20 minute incubation with mixing in 30% bleach, 0.01% triton-X 100, (3) 5× rinses with sterile water, (4) Seeds were re-suspended in 0.1% sterile agarose and stratified at 4° C. for 3-4 days.

All germination assays follow modifications of the same basic protocol. Sterile seeds were sown on the conditional media that had a basal composition of 80% MS+Vitamins. Plates were incubated at 22° C. under 24-hour light (120-130 μE m⁻² s⁻¹) in a growth chamber. Evaluation of germination and seedling vigor was performed 5 days after planting. For assessment of root development, seedlings germinated on 80% MS+Vitamins+1% sucrose were transferred to square plates at 7 days. Evaluation was done 5 days after transfer following growth in a vertical position. Qualitative differences were recorded including lateral and primary root length, root hair number and length, and overall growth.

For chilling (8° C.) and heat sensitivity (32° C.) growth assays, seeds were germinated and grown for 7 days on MS+Vitamins+1% sucrose at 22° C. and then were transferred to chilling or heat stress conditions. Heat stress was applied for 5 days, after which the plants were transferred back to 22° C. for recovery and evaluated after a further 5 days. Plants were subjected to chilling conditions (8° C.) and evaluated at 10 days and 17 days.

For plate-based severe dehydration assays (sometimes referred to as desiccation assays), seedlings were grown for 14 days on MS+Vitamins+1% Sucrose at 22° C. Plates were opened in the sterile hood for 3 hr for hardening and then seedlings were removed from the media and dried for 2 h in the hood. After this time they were transferred back to plates and incubated at 22° C. for recovery. Plants were evaluated after another 5 days.

Data Interpretation

At the time of evaluation, plants were given one of the following scores:

-   (++) Substantially enhanced performance compared to controls. The     phenotype was very consistent and growth was significantly above the     normal levels of variability observed for that assay. -   (+) Enhanced performance compared to controls. The response was     consistent but was only moderately above the normal levels of     variability observed for that assay. -   (wt) No detectable difference from wild-type controls. -   (−) Impaired performance compared to controls. The response was     consistent but was only moderately above the normal levels of     variability observed for that assay. -   (−−) Substantially impaired performance compared to controls. The     phenotype was consistent and growth was significantly above the     normal levels of variability observed for that assay. -   (n/d) Experiment failed, data not obtained, or assay not performed.

Example VII Soil Drought Clay Pot

The soil drought assay (performed in clay pots) was based on that described by Haake et al. (2002).

Experimental Procedure.

Previously, we performed clay-pot assays on segregating T2 populations, sown directly to soil. However, in the current procedure, seedlings were first germinated on selection plates containing either kanamycin or sulfonamide.

Seeds were sterilized by a 2 minute ethanol treatment followed by 20 minutes in 30% bleach/0.01% Tween and five washes in distilled water. Seeds were sown to MS agar in 0.1% agarose and stratified for 3 days at 4° C., before transfer to growth cabinets with a temperature of 22° C. After 7 days of growth on selection plates, seedlings were transplanted to 3.5 inch diameter clay pots containing 80 g of a 50:50 mix of vermiculite:perlite topped with 80 g of ProMix. Typically, each pot contains 14 seedlings, and plants of the transgenic line being tested were in separate pots to the wild-type controls. Pots containing the transgenic line versus control pots were interspersed in the growth room, maintained under 24-hour light conditions (18-23° C., and 90-100 μE m⁻² s⁻¹) and watered for a period of 14 days. Water was then withheld and pots were placed on absorbent paper for a period of 8-10 days to apply a drought treatment. After this period, a visual qualitative “drought score” from 0-6 was assigned to record the extent of visible drought stress symptoms. A score of “6” corresponded to no visible symptoms whereas a score of “0” corresponded to extreme wilting and the leaves having a “crispy” texture. At the end of the drought period, pots were re-watered and scored after 5-6 days; the number of surviving plants in each pot was counted, and the proportion of the total plants in the pot that survived was calculated.

Split-pot method. A variation of the above method was sometimes used, whereby plants for a given transgenic line were compared to wild-type controls in the same pot. For those studies, 7 wild-type seedlings were transplanted into one half of a 3.5 inch pot and 7 seedlings of the line being tested were transplanted into the other half of the pot.

Analysis of results. In a given experiment, we typically compared six or more pots of a transgenic line with 6 or more pots of the appropriate control. (In the split pot method, 12 or more pots were used.) The mean drought score and mean proportion of plants surviving (survival rate) were calculated for both the transgenic line and the wild-type pots. In each case a p-value* was calculated, which indicated the significance of the difference between the two mean values. The results for each transgenic line across each planting for a particular project were then presented in a results table.

Calculation of p-values. For the assays where control and experimental plants were in separate pots, survival was analyzed with a logistic regression to account for the fact that the random variable was a proportion between 0 and 1. The reported p-value was the significance of the experimental proportion contrasted to the control, based upon regressing the logit-transformed data.

Drought score, being an ordered factor with no real numeric meaning, was analyzed with a non-parametric test between the experimental and control groups. The p-value was calculated with a Mann-Whitney rank-sum test.

For the split-pot assays, matched control and experimental measurements were available for both variables. In lieu of a direct transformed regression technique for these data, the logit-transformed proportions were analyzed by parametric methods. The p-value was derived from a paired-t-test on the transformed data. For the paired score data, the p-value from a Wilcoxon test was reported.

Example VIII Soil Drought Single Pot

These experiments determined the physiological basis for the drought tolerance conferred by each lead and were typically performed under soil grown conditions. Usually, the experiment was performed under photoperiodic conditions of 10-hr or 12-hr light. Where possible, a given project (gene/promoter combination or protein variant) was represented by three independent lines. Plants were usually at late vegetative/early reproductive stage at the time measurements were taken. Typically we assayed three different states: a well-watered state, a mild-drought state and a moderately severe drought state. In each case, we made comparisons to wild-type plants with the same degree of physical stress symptoms (wilting). To achieve this, staggered samplings were often required. Typically, for a given line, ten individual plants were assayed for each state.

The following physiological parameters were routinely measured: relative water content, ABA content, proline content, and photosynthesis rate. In some cases, measurements of chlorophyll levels, starch levels, carotenoid levels, and chlorophyll fluorescence were also made.

Analysis of results. In a given experiment, for a particular parameter, we typically compared about 10 samples from a given transgenic line with about 10 samples of the appropriate wild-type control at each drought state. The mean values for each physiological parameter were calculated for both the transgenic line and the wild-type pots. In each case, a P-value (calculated via a simple t-test) was determined, which indicated the significance of the difference between the two mean values. The results for each transgenic line across each planting for a particular project were then presented in a results table.

A typical procedure is described below; this corresponds to method used for the drought time-course experiment which we performed on wild-type plants during our baseline studies at the outset of the drought program.

Procedure. Seeds were stratified for 3 days at 4° C. in 0.1% agarose and sown on Metromix 200 in 2.25 inch pots (square or round). Plants were maintained in individual pots within flats grown under short days (10:14 L:D). Seedlings were watered as needed to maintain healthy plant growth and development. At 7 to 8 weeks after planting, plants were used in drought experiments.

Plants matched for equivalent growth development (rosette size) were removed from plastic flats and placed on absorbent paper. Pots containing plants used as well-watered controls were placed within a weigh boat and the dish placed on the absorbent paper. The purpose of the weigh boat was to retain any water that might leak from well-watered pots and affect pots containing plants undergoing the drought stress treatment.

On each day of sampling, up to 18 droughted plants and 6 well-watered controls (from each transgenic line) were picked from a randomly generated pool (given that they passed quality control standards). Biochemical analysis for photosynthesis, ABA, and proline was performed on the next three youngest, most fully expanded leaves. Relative water content was analyzed using the remaining rosette tissue.

Example IX Soil Drought Biochemical and Physiological Assays

Background. The purpose of these measurements was to determine the physiological state of plants in soil drought experiments.

Measurement of Photosynthesis. Photosynthesis was measured using a LICOR LI-6400. The LI-6400 uses infrared gas analyzers to measure carbon dioxide to generate a photosynthesis measurement. This method is based upon the difference of the CO₂ reference (the amount put into the chamber) and the CO₂ sample (the amount that leaves the chamber). Since photosynthesis is the process of converting CO₂ to carbohydrates, we expected to see a decrease in the amount of CO₂ sample. From this difference, a photosynthesis rate can be generated. In some cases, respiration may occur and an increase in CO₂ detected. To perform measurements, the LI-6400 was set-up and calibrated as per LI-6400 standard directions. Photosynthesis was measured in the youngest most fully expanded leaf at 300 and 1000 ppm CO₂ using a metal halide light source. This light source provided about 700 μE m⁻² s⁻¹.

Fluorescence was measured in dark and light adapted leaves using either a LI-6400 (LICOR) with a leaf chamber fluorometer attachment or an OS-1 (Opti-Sciences, Hudson, N.H.) as described in the manufacturer's literature. When the LI-6400 was used, all manipulations were performed under a dark shade cloth. Plants were dark adapted by placing in a box under this shade cloth until used. The OS-30 utilized small clips to create dark adapted leaves.

Measurement of Abscisic Acid and Proline. The purpose of this experiment was to measure ABA and proline in plant tissue. ABA is a plant hormone believed to be involved in stress responses and proline is an osmoprotectant.

Three of the youngest, most fully expanded mature leaves were harvested, frozen in liquid nitrogen, lyophilized, and a dry weight measurement taken. Plant tissue was then homogenized in methanol to which 500 ng of d6-ABA had been added to act as an internal standard. The homogenate was filtered to removed plant material and the filtrate evaporated to a small volume. To this crude extract, approximately 3 ml of 1% acetic acid was added and the extract was further evaporated to remove any remaining methanol. The volume of the remaining aqueous extract was measured and a small aliquot (usually 200 to 500 μl) removed for proline analysis (Protocol described below). The remaining extract was then partitioned twice against ether, the ether removed by evaporation and the residue methylated using ethereal diazomethane. Following removal of any unreacted diazomethane, the residue was dissolved in 100 to 200 μl ethyl acetate and analyzed by gas chromatography-mass spectrometry. Analysis was performed using an HP 6890 GC coupled to an HP 5973 MSD using a DB-5 ms gas capillary column. Column pressure was 20 psi. Initially, the oven temperature was 150° C. Following injection, the oven was heated at 5° C./min to a final temperature of 250° C. ABA levels were estimated using an isotope dilution equation and normalized to tissue dry weight.

Free proline content was measured according to Bates (Bates et al., 1973). The crude aqueous extract obtained above was brought up to a final volume of 500 μl using distilled water. Subsequently, 500 μl of glacial acetic was added followed by 500 μl of Chinard's Ninhydrin. The samples were then heated at 95 to 100° C. for 1 hour. After this incubation period, samples were cooled and 1.5 ml of toluene were added. The upper toluene phase was removed and absorbance measured at 515 nm. Amounts of proline were estimated using a standard curve generated using L-proline and normalized to tissue dry weight.

[n.b. Chinard's Ninhydrin was prepared by dissolving 2.5 g ninhydrin (triketohydrindene hydrate) in 60 ml glacial acetic acid at 70° C. to which 40 ml of 6 M phosphoric acid was added.]

Measurement of Relative Water Content (RWC). Relative Water Content (RWC) indicates the amount of water that is stored within the plant tissue at any given time. It was obtained by taking the field weight of the rosette minus the dry weight of the plant material and dividing by the weight of the rosette saturated with water minus the dry weight of the plant material. The resulting RWC value can be compared from plant to plant, regardless of plant size.

${{Relative}\mspace{14mu} {Water}\mspace{14mu} {Content}} = {\frac{{{Field}\mspace{14mu} {Weight}} - {{Dry}\mspace{14mu} {Weight}}}{{{Turgid}\mspace{14mu} {Weight}} - {{Dry}\mspace{14mu} {Weight}}} \times 100}$

After tissue had been removed for array and ABA/proline analysis, the rosette was cut from the roots using a small pair of scissors. The field weight was obtained by weighing the rosette. The rosette was then immersed in cold water and placed in an ice water bath in the dark. The purpose of this was to allow the plant tissue to take up water while preventing any metabolism which could alter the level of small molecules within the cell. The next day, the rosette was carefully removed, blotted dry with tissue paper, and weighed to obtain the turgid weight. Tissue was then frozen, lyophilized, and weighed to obtain the dry weight.

Starch determination. Starch was estimated using a simple iodine based staining procedure. Young, fully expanded leaves were harvested either at the end or beginning of a 12 h light period and placed in tubes containing 80% ethanol or 100% methanol. Leaves were decolorized by incubating tubes in a 70 to 80 C water bath until chlorophyll had been removed from leaf tissue. Leaves were then immersed in water to displace any residual methanol which may be present in the tissue. Starch was then stained by incubating leaves in an iodine stain (2 g KI, 1 g I₂ in 100 ml water) for one min and then washing with copious amounts of water. Tissue containing large amounts of starch stained dark blue or black; tissues depleted in starch were colorless.

Chlorophyll/carotenoid determination. For some experiments, chlorophyll was estimated in methanolic extracts using the method of Porra et al. (1989). Carotenoids were estimated in the same extract at 450 nm using an A(1%) of 2500. Chlorophyll was measured with a SPAD-502 (Minolta). Both carotenoid and chlorophyll content and amount could also be determined via HPLC. In this procedure pigments were extracted from leave tissue by homogenizing leaves in acetone:ethyl acetate (3:2). Water was added, the mixture centrifuged, and the upper phase removed for HPLC analysis. Samples were analyzed using a Zorbax C18 (non-endcapped) column (250×4.6) with a gradient of acetonitrile:water (85:15) to acetonitrile:methanol (85:15) in 12.5 minutes. After holding at these conditions for two minutes, solvent conditions were changed to methanol:ethyl acetate (68:32) in two minutes. Carotenoids and chlorophylls were quantified using peak areas and response factors calculated using lutein and beta-carotene as standards.

Quantification of protein level. Protein level quantification was performed for 35S::G481 and related projects. Plants were plated on selective MS media, and transplanted to vertical MS plates after one week of growth. After 17 days of growth (24 h light, 22 C), tissues were harvested from the vertical plates. The shoot tissue from 1 plant was harvested as one biological replicate for each line, and the root tissue from 2 plants were combined as I biological replicate. For each line analyzed, two biological replicates each of shoot and root tissue were analyzed. Whole cell protein extracts were prepared in a 96 well format and separated on a 4-20% SDS-PAGE gel, transferred to PVDF membrane for western blotting, and probed with a 1:2000 dilution of anti-G481 antibody in a 1% blocking solution in TBS-T. Protein levels for various samples were estimated by setting a level of one for pMEN65 wild type and three for line G481-6 to describe the amount of G481 protein visible on the blot. The protein level for each of the other lines tested was visually estimated on each blot relative to the pMEN65 and G481-6 standards.

Nuclear and cytoplasmically-enriched fractions. We developed a platform to prepare nuclear and cytoplasmic protein extracts in a 96-well format using a tungsten carbide beads for cell disruption in a mild detergent and a sucrose cushion to separate cytoplasmic from nuclear fractions. We used histone antibodies to demonstrate that this method effectively separated cytoplasmic from nuclear-enriched fractions. An alternate method (spun only) used the same disruption procedure, but simply pelleted the nuclei to separate them from the cytoplasm without the added purification of a sucrose cushion.

Quantification of mRNA level. Three shoot and three root biological replicates were typically harvested for each line, as described above in the protein quantification methods section. RNA was prepared using a 96-well format protocol, and cDNA synthesized from each sample. These preparations were used as templates for RT-PCR experiments. We measured the levels of transcript for a gene of interest (such as G481) relative to 18S RNA transcript for each sample using an ABI 7900 Real-Time RT-PCR machine with SYBR Green technology.

Phenotynic Analysis: Flowering time. Plants were grown in soil. Flowering time was determined based on either or both of (i) number to days after planting to the first visible flower bud. (ii) the total number of leaves (rosette or rosette plus cauline) produced by the primary shoot meristem.

Phenotynic Analysis: Heat stress. In preliminary experiments described in this report, plants were germinated growth chamber at 30 C with 24 h light for 11 d. Plants were allowed to recover in 22 C with 24 h light for three days, and photographs were taken to record health after the treatment. In a second experiment, seedlings were grown at 22 C for four days on selective media, and the plates transferred to 32 C for one week. They were then allowed to recover at 22 C for three days. Forty plants from two separate plates were harvested for each line, and both fresh weight and chlorophyll content measured.

Phenotypic Analysis: Dark-induced senescence. In preliminary experiments described in this report, plants were grown on soil for 27-30 days in 12 h light at 22 C. They were moved to a dark chamber at 22 C, and visually evaluated for senescence after 10-13 days. In some cases we used Fv/Fm as a measure of chlorophyll (Pourtau et al., 2004) on the youngest most fully-expanded leaf on each plant. The Fv/Fm mean for the 12 plants from each line was normalized to the Fv/Fm mean for the 12 matched controls.

Various Definitions Used in this Report: RWC Relative water content (field wt.-dry weight)/(turgid wt.-dry wt.)×100 ABA=Abscisic acid, μg/gdw Proline=Proline, μmole/gdw A 300=net assimilation rate, μmole CO₂/m²/s at 300 ppm CO₂ A 1000=net assimilation rate, μmole CO₂/m²/s at 1000 ppm CO₂ Chl SPAD=Chlorophyll estimated by a Minolta SPAD-502, ratio of 650 nm to 940 nm Total Chl=mg/gfw, estimated by HPLC Carot=mg/gfw, estimated by HPLC Fo=minimal fluorescence of a dark adapted leaf Fm=maximal fluorescence of a dark adapted leaf Fo′=minimal fluorescence of a light adapted leaf Fm′=maximal fluorescence of a light adapted leaf Fs=steady state fluorescence of a light adapted leaf Psi If=water potential (Mpa) of a leaf Psi p=turgor potential (Mpa) of a leaf Psi pi=osmotic potential (Mpa) of a leaf Fv/Fm=(Fm−Fo)/Fm; maximum quantum yield of PSII Fv′/Fm′=(Fm′−Fo′)/Fm′; efficiency of energy harvesting by open PSII reaction centers PhiPS2=(Fm′−Fs)/Fm′, actual quantum yield of PSII ETR=PhiPS2×light intensity absorbed×0.5; we use 100 μE/m²/s for an average light intensity and 85% as the amount of light absorbed qP=(Fm′−Fs)/(Fm′−Fo′); photochemical quenching (includes photosynthesis and photorespiration); proportion of open PSII qN=(Fm−Fm′)/(Fm−Fo′); non-photochemical quenching (includes mechanisms like heat dissipation) NPQ=(Fm−Fm′)/Fm′; non-photochemical quenching (includes mechanisms like heat dissipation)

Example X Disease Physiology, Plate Assays

Overview. A Sclerotinia plate-based assay was used as a pre-screen to identify top performing lines from each project (i.e., lines from transformation with a particular construct) that could be tested in subsequent soil-based assays. Top performing lines were also subjected to Botrytis cinerea plate assays as noted. Typically, eight lines were subjected to plate assays, from which the best lines were selected for subsequent soil-based assays. In projects where significant pathogen resistance was not obtained in plate based assays, lines were not submitted for soil assays.

Unless otherwise stated, all experiments were performed with the Arabidopsis thaliana ecotype Columbia (Col-0). Similar assays could be devised for other crop plants such as soybean or maize plants. Assays were usually performed on non-selected segregating T2 populations (in order to avoid the extra stress of selection). Control plants for assays on lines containing direct promoter-fusion constructs were wild-type plants or Col-0 plants transformed an empty transformation vector (pMEN65). Controls for 2-component lines (generated by supertransformation) were the background promoter-driver lines (i.e. promoter::LexA-GAL4TA lines), into which the supertransformations were initially performed.

Procedures. Prior to plating, seed for all experiments were surface sterilized in the following manner: (1) 5 minute incubation with mixing in 70% ethanol; (2) 20 minute incubation with mixing in 30% bleach, 0.01% Triton X-100; (3) five rinses with sterile water. Seeds were resuspended in 0.1% sterile agarose and stratified at 4° C. for 24 days.

Sterile seeds were sown on starter plates (15 mm deep) containing the following medium: 50% MS solution, 1% sucrose, 0.05% MES, and 1% Bacto-Agar. 40 to 50 seeds were sown on each plate. Plates were incubated at 22° C. under 24-hour light (95-110 μm⁻² s⁻¹) in a germination growth chamber. On day 10, seedlings were transferred to assay plates (25 mm deep plates with medium minus sucrose). Each assay plate had nine test seedlings and nine control seedlings on separate halves of the plate. Three or four plates were used per line, per pathogen. On day 14, seedlings were inoculated (specific methods below). After inoculation, plates were put in a growth chamber under a 12-hour light/12-hour dark schedule. Light intensity was lowered to 70-80 μE m⁻² s⁻¹ for the disease assay. Disease symptoms were evaluated starting four days post-inoculation (DPI) up to 10 DPI if necessary. For each plate, the number of dead test plants and control plants were counted. Plants were scored as “dead” if the center of the rosette collapsed (usually brown or water-soaked).

Sclerotinia inoculum preparation. A Sclerotinia liquid culture was started three days prior to plant inoculation by cutting a small agar plug (¼ sq. inch) from a 14- to 21-day old Sclerotinia plate (on Potato Dextrose Agar; PDA) and placing it into 100 ml of half-strength Potato Dextrose Broth (PDB). The culture was allowed to grown in the PDB at room temperature under 24-hour light for three days. On the day of seedling inoculation, the hyphal ball was retrieved from the medium, weighed, and ground in a blender with water (50 ml/gm tissue). After grinding, the mycelial suspension was filtered through two layers of cheesecloth and the resulting suspension was diluted 1:5 in water. Plants were inoculated by spraying to run-off with the mycelial suspension using a Preval aerosol sprayer.

Botrytis inoculum preparation. Botrytis inoculum was prepared on the day of inoculation. Spores from a 14- to 21-day old plate were resuspended in a solution of 0.05% glucose, 0.03M KH₂PO₄ to a final concentration of 10⁴ spores/ml. Seedlings were inoculated with a Preval aerosol sprayer, as with Sclerotinia inoculation.

Data Interpretation. After the plates were evaluated, each line was given one of the following qualitative scores:

(++) Substantially enhanced resistance compared to controls. The phenotype was very consistent across all plates for a given line.

(+) Enhanced resistance compared to controls. The response was consistent but was only moderately above the normal levels of variability observed for that assay.

(wt) No detectable difference from wild-type controls.

(−) Increased susceptibility compared to controls. The response was consistent but was only moderately above the normal levels of variability observed for that assay.

(−−) Substantially impaired performance compared to controls. The phenotype was consistent and growth was significantly above the normal levels of variability observed for that assay.

(n/d) Experiment failed, data not obtained, or assay not performed.

Example XI Disease Physiology, Soil Assays

Overview. Lines from transformation with a particular construct were tested in a soil-based assay for resistance to powdery mildew (Erysiphe cichoracearum) as noted below. Typically, eight lines per project were subjected to the Erysiphe assay.

Unless otherwise stated, all experiments were performed with the Arabidopsis thaliana ecotype Columbia (Col-0). Assays were usually performed on non-selected segregating T2 populations (in order to avoid the extra stress of selection). Control plants for assays on lines containing direct promoter-fusion constructs were wild-type plants or Col-0 plants transformed an empty transformation vector (pMEN65). Controls for 2-component lines (generated by supertransformation) were the background promoter-driver lines (i.e. promoter::LexA-GAL4TA lines), into which the supertransformations were initially performed.

In addition, positive hits from the Sclerotinia plate assay were subjected to a soil-based Sclerotinia assay as noted. This assay was based on hyphal plug inoculation of rosette leaves.

Procedures. Erysiphe inoculum was propagated on a pad4 mutant line in the Col-0 background, which is highly susceptible to Erysiphe (Reuber et al., 1998). The inoculum was maintained by using a small paintbrush to dust conidia from a 2-3 week old culture onto new plants (generally three weeks old). For the assay, seedlings were grown on plates for one week under 24-hour light in a germination chamber, then transplanted to soil and grown in a walk-in growth chamber under a 12-hour light/12-hour dark light regimen, 70% humidity. Each line was transplanted to two 13 cm square pots, nine plants per pot. In addition, three control plants were transplanted to each pot for direct comparison with the test line. Approximately 3.5 weeks after transplanting, plants were inoculated using settling towers as described by Reuber et al. (1998). Generally, three to four heavily infested leaves were used per pot for the disease assay. The level of fungal growth was evaluated eight to ten days after inoculation.

Data Interpretation. After the pots were evaluated, each line was given one of the following overall scores:

(+++) Highly enhanced resistance as compared to controls. The phenotype was very consistent.

(++) Substantially enhanced resistance compared to controls. The phenotype was very consistent in both pots for a given line.

(+) Enhanced resistance compared to controls. The response was consistent but was only moderately above the normal levels of variability observed.

(wt) No detectable difference from wild-type controls.

(−) Increased susceptibility compared to controls. The response was consistent but was only moderately above the normal levels of variability observed.

(−−) Substantially impaired performance compared to controls. The phenotype was consistent and growth was significantly above the normal levels of variability observed.

(n/d) Experiment failed, data not obtained, or assay not performed.

Example XII Experimental Results

This application provides experimental observations for a number of transcription factors for improved yield and/or increased tolerance to abiotic stresses such as water deficit-related tolerance, low nutrient tolerance, cold tolerance (for example, G481, G682, G867, G1073, G28, G47, G1274, G1792, G2999, G3086, G1988, G207, G922, G1760, and G2053 (SEQ ID NOs: 10, 550, 16, 18, 2, 6, 20, 24, 1794, 1836, 30, 178, 690, 22, and 1336, respectively), two transcription factors for disease resistance (G28, SEQ ID NO: 2, and G1792, SEQ ID NO: 24), and, for each of these transcription factors, a number of phylogenetically and closely related homologs derived from diverse gene sequences. A set of polynucleotides and polypeptides related to each lead transcription factor has been designated as a “study group” and related sequences in these clades have been subsequently analyzed using morphological and phenotypic studies.

Phenotypic Screens: promoter combinations. A panel of promoters was assembled based on domains of expression that had been well characterized in the published literature. These were chosen to represent broad non-constitutive patterns which covered the major organs and tissues of the plant. The following domain-specific promoters were picked, each of which drives expression in a particular tissue or cell-type: ARSK1 (root), RBCS3 (photosynthetic tissue, including leaf tissue), CUT1 (shoot epidermal, guard-cell enhanced), SUC2 (vascular), STM (apical meristem and mature-organ enhanced), AP1 (floral meristem enhanced), AS1 (young organ primordia) and RSI1 (young seedlings, and roots). Also selected was a stress inducible promoter, RD29A, which is able to up-regulate a transgene at drought onset.

The basic strategy was to test each polynucleotide with each promoter to give insight into the following questions: (i) mechanistically, in which part of the plant is activity of the polynucleotide sufficient to produce stress tolerance? (ii) Can we identify expression patterns which produce compelling stress tolerance while eliminating any undesirable effects on growth and development? (iii) Does a particular promoter give an enhanced or equivalent stress tolerance phenotype relative to constitutive expression? Each of the promoters in this panel is considered to be representative of a particular pattern of expression; thus, for example, if a particular promoter such as SUC2, which drives expression in vascular tissue, yields a positive result with a particular transcription factor gene, it would be predicted and expected that a positive result would be obtained with any other promoter that drives the same vascular pattern.

We now have many examples demonstrating the principle that use of a regulated promoter can confer substantial stress tolerance while minimizing deleterious effects. For example, the results from regulating G1792-related genes using regional specific promoters were especially persuasive. When overexpressed constitutively, these genes produced extreme dwarfing. However, when non-constitutive promoters were used to express these sequences ectopically, off-types were substantially ameliorated, and strong disease tolerance was still obtained (for example, with RBCS3::G1792 and RBCS3::G1795 lines). Another project worth highlighting is ARSK1::G867 where expression in the roots yielded drought tolerance without any apparent off-types.

Additionally, it is feasible to identify promoters which afford high levels of inducible expression. For instance, a major tactic in the disease program is to utilize pathogen inducible promoters; a set of these has now been identified for testing with each of the disease-resistance conferring transcription factors. This approach is expected to be productive as we have shown that inducible expression of G1792 via the dexamethasone system gives effective disease tolerance without off-types. By analogy, it would be useful to take a similar approach for the drought tolerance trait. So far the only drought regulated promoter that we have tested is RD29A, since its utility had been published (Kasuga et al., 1999).

Phenotypic Screens: effects of protein variants for distinct transcription factors. The effects of overexpressing a variety of different types of protein variants including: deletion variants, GAL4 fusions, variants with specific residues mutagenized, and forms in which domains were swapped with other proteins, have been examined. Together, these approaches have been informative, and have helped illuminate the role of specific residues (see for example, the site-directed mutagenesis experiments for G1274 or G1792), as well as giving new clues as to the basis of particular phenotypes. For example, overexpression lines for a G481 deletion variant exhibited drought tolerance, suggesting that the G481 drought phenotype might arise from dominant negative type interactions.

Phenotynic Screens: knockout and knock-down approaches. Thus far, both T-DNA alleles and RNAi methods have been used to isolate knockouts/knockdown lines for transcription factors of interest. In general, it was determined that the knockout (KO) approach to be more informative and easier to interpret than RNAi based strategies. In particular, RNAi approaches are hampered by the possibility that other related transcription factors might be directly or indirectly knocked-down (even when using a putative gene-specific construct). Thus, a set of RNAi lines showing an interesting phenotype requires a very substantial amount of molecular characterization to prove that the phenotypes are due to reduced activity of the targeted gene. We have found that KO lines have given some useful insights into the relative endogenous roles of particular genes within the CAAT family, and revealed the potential for obtaining stress tolerance traits via knock-down strategies (e.g., G481 knockout/knockdown approaches).

Table 36 summarizes experimental results with plants in which sequences of the invention have either been overexpressed, reduced, or knocked out. These modifications have yielded new and potentially valuable phenotypic traits, relative to control plants, in morphological, physiological or disease assays, as demonstrated in Arabidopsis, or alternatively in tomato or other plants where noted. The last column lists the trait that was experimentally observed in plants, relative to control plants, after: either transforming plants with each transcription factor polynucleotide GID (Gene IDentifier, found in the first column) under the listed regulatory control mechanism; or (ii) in the cases where the project is listed as a knockout, expression of the transcription factor was abolished; or (iii) in the cases where the project is listed as “RNAi (GS) or RNAi(clade), the transcription factor was knocked down using RNAi targeting either the gene sequence or the clade of related genes, respectively.

TABLE 36 Phenotypic traits conferred by transcription factors in morphological, physiological or disease assays TF family (amino acid coordinates SEQ of ID Phylogenetic characteristic NO: relationship; Construct SEQ ID Experimental conserved of closely related containing NO: of observation (trait GID domain) GID to: Expression system TF Construct compared to controls) G7 AP2 (58-125) 40 G7 Const. 35S prom. P165 3850 Greater tol. to dehydration G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 More tol. to drought* and better recovery from drought treatment* G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Late flowering G28 AP2 (145-208) 2 G28 Const. 35S prom. P26537 5019 Late flowering G28 AP2 (145-208) 2 G28 Const. 35S prom. P26378 4967 Late flowering G28 AP2 (145-208) 2 G28 2 comp. including P7826 4605 Late flowering P6506 (35S prom.) G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Darker green leaf color G28 AP2 (145-208) 2 G28 Const. 35S prom. P26537 5019 Darker green leaf color G28 AP2 (145-208) 2 G28 Const. 35S prom. P26378 4967 Darker green leaf color G28 AP2 (145-208) 2 G28 2 comp. including P7826 4605 Darker green leaf color P6506 (35S prom.) G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Greater resistance to Botrytis G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Greater resistance to Sclerotinia G28 AP2 (145-208) 2 G28 Const. 35S prom. P174 3854 Greater resistance to Erysiphe G28 AP2 (145-208) 2 G28 Root-specific P23541 4845 Early flowering ARSK1 prom. G28 AP2 (145-208) 2 G28 Epidermal-specific P23441 4835 Greater res. to CUT1 prom. Erysiphe G28 AP2 (145-208) 2 G28 Epidermal and P23543 4846 Greater res. to vascular-specific Erysiphe LTP1 prom. G28 AP2 (145-208) 2 G28 Leaf-specific P23544 4847 Greater res. to RBCS3 prom. Erysiphe G28 AP2 (145-208) 2 G28 Leaf-specific P23544 4847 Darker green leaf color RBCS3 prom. G28 AP2 (145-208) 2 G28 Protein-GFP C P26497 5015 Greater res. to terminal fusion, Sclerotinia 35S G1006 AP2 (113-177) 752 G28 Const. 35S prom. P417 3931 Greater res. to Erysiphe G1006 AP2 (113-177) 752 G28 Const. 35S prom. P417 3931 Greater res. to Sclerotinia G1006 AP2 (113-177) 752 G28 Const. 35S prom. P417 3931 Darker green leaf color G22 AP2 (88-152) 56 G28 Const. 35S prom. P806 3977 Late flowering G22 AP2 (88-152) 56 G28 Const. 35S prom. P806 3977 Greater tol. to NaCl (determined with 150 mM NaCl) G22 AP2 (88-152) 56 G28 2 comp. including P3376 4509 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G22 AP2 (88-152) 56 G28 2 comp. including P3376 4509 Significantly greater P5318 (STM soluble solids (Brix) in prom.) tomato plants G22 AP2 (88-152) 56 G28 2 comp. including P3376 4509 Significantly greater P5284 (RBCS3 lycopene in tomato prom.) plants G3659 AP2 (130-194) 2054 G28 Const. 35S prom. P23452 4836 Greater res. to Erysiphe G3659 AP2 (130-194) 2054 G28 Const. 35S prom. P23452 4836 Late flowering G3659 AP2 (130-194) 2054 G28 Const. 35S prom. P23452 4836 Greater res. to Sclerotinia G3659 AP2 (130-194) 2054 G28 Const. 35S prom. P23452 4836 Glossy leaves G3659 AP2 (130-194) 2054 G28 Const. 35S prom. P23452 4836 Darker green leaf color G3660 AP2 (119-183) 2056 G28 Const. 35S prom. P23418 4831 Glossy leaves G3660 AP2 (119-183) 2056 G28 Const. 35S prom. P23418 4831 Late flowering G3660 AP2 (119-183) 2056 G28 Const. 35S prom. P23418 4831 Greater res. to Sclerotinia G3660 AP2 (119-183) 2056 G28 Const. 35S prom. P23418 4831 Greater res. to Botrytis G3660 AP2 (119-183) 2056 G28 Const. 35S prom. P23418 4831 Greater res. to Erysiphe G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Greater res. to Erysiphe G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Greater res. to Sclerotinia G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Late flowering G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Glossy leaves G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Darker green leaf color G3717 AP2 (130-194) 2076 G28 Const. 35S prom. P23421 4833 Altered C/N sensing: Greater tol. to low nitrogen conditions in C/N sensing assay G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Greater res. to Erysiphe G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Greater res. to Sclerotinia G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Glossy leaves G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Darker green leaf color G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Late flowering G3718 AP2 (139-203) 2078 G28 Const. 35S prom. P23423 4834 Altered C/N sensing: Greater tol. to low nitrogen conditions in C/N sensing assay G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Greater res. to Erysiphe G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Greater res. to Erysiphe G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Greater res. to Sclerotinia G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Greater res. to Sclerotinia G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Late flowering G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Late flowering G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Altered leaf shape G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Altered leaf shape G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Glossy leaves G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Glossy leaves G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Darker green leaf color G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Darker green leaf color G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P25573 4912 Altered C/N sensing: Greater tol. to low nitrogen conditions in C/N sensing assay G3841 AP2 (102-166) 2158 G28 Const. 35S prom. P26576 5021 Altered C/N sensing: Greater tol. to low nitrogen conditions in C/N sensing assay G3843 AP2 (130-194) 2160 G28 — — n/d G3852 AP2 (102-167) 2170 G28 — — n/d G3844 AP2 (141-205) 2162 G28 — — n/d G3845 AP2 (101-165) 2164 G28 — — n/d G3846 AP2 (95-159) 2166 G28 — — n/d G3857 AP2 (98-162) 2174 G28 — — n/d G3858 AP2 (108-172) 2176 G28 — — n/d G3430 AP2 (145-209) 4 G28 Const. 35S prom. P21267 4768 Greater res. to Erysiphe G3430 AP2 (145-209) 4 G28 Const. 35S prom. P21267 4768 Greater res. to Sclerotinia G3430 AP2 (145-209) 4 G28 Const. 35S prom. P21267 4768 Late flowering G3430 AP2 (145-209) 4 G28 Const. 35S prom. P21267 4768 Darker green leaf color G3848 AP2 (149-213) 2168 G28 Const. 35S prom. P25571 4910 Greater res. to Erysiphe G3848 AP2 (149-213) 2168 G28 Const. 35S prom. P25571 4910 Greater res. to Sclerotinia G3848 AP2 (149-213) 2168 G28 Const. 35S prom. P25571 4910 Late flowering G3848 AP2 (149-213) 2168 G28 Const. 35S prom. P25571 4910 Glossy leaves G3848 AP2 (149-213) 2168 G28 Const. 35S prom. P25571 4910 Darker green leaf color G3856 AP2 (140-204) 2172 G28 Const. 35S prom. P25572 4911 Greater res. to Erysiphe G3856 AP2 (140-204) 2172 G28 Const. 35S prom. P25572 4911 Greater res. to Sclerotinia G3856 AP2 (140-204) 2172 G28 Const. 35S prom. P25572 4911 Glossy leaves G3856 AP2 (140-204) 2172 G28 Const. 35S prom. P25572 4911 Darker green leaf color G3661 AP2 (126-190) 2058 G28 Const. 35S prom. P23419 4832 Greater res. to Erysiphe G3661 AP2 (126-190) 2058 G28 Const. 35S prom. P23419 4832 Late flowering G3661 AP2 (126-190) 2058 G28 Const. 35S prom. P23419 4832 Glossy leaves G3864 AP2 (127-191) 2178 G28 — — n/d G3865 AP2 (125-189) 2180 G28 — — n/d G45 AP2 (152-217) 76 G45 Domain P25440 4905 Greater res. to swap/chimeric Erysiphe variant, 35S G45 AP2 (152-217) 76 G45 Domain P25440 4905 Late flowering swap/chimeric variant, 35S G45 AP2 (152-217) 76 G45 Domain P25440 4905 Darker green leaf color swap/chimeric variant, 35S G45 AP2 (152-217) 76 G45 Domain P25440 4905 C/N sensing: greater swap/chimeric sens. to low nitrogen variant, 35S conditions G45 AP2 (152-217) 76 G45 Domain P25440 4905 Greater res. to swap/chimeric Sclerotinia variant, 35S G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 More lignin G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 More lignin P6506 (35S prom.) G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 Altered stem morphology; wider stem diameter, large irregular vascular bundles with a much greater number of xylem vessels; xylem vessels within the bundles appeared narrow and more lignified G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Altered stem P6506 (35S prom.) morphology; wider stem diameter, large irregular vascular bundles with a much greater number of xylem vessels; xylem vessels within the bundles appeared narrow and more lignified G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 Better root growth under hyperosmotic stress with PEG G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Better root growth P6506 (35S prom.) under hyperosmotic stress with PEG G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 Late flowering G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Late flowering P5284 (RBCS3 prom.) G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 Altered architecture and inflorescence development; thick, fleshy inflorescences, reduced apical dominance, reduced internode elongation, stem branching pattern altered - primary shoot ‘kinked’ at each coflorescence node, reduced fertility, small siliques borne on short pedicels held vertically and close against the stem G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Altered architecture P6506 (35S prom.) and inflorescence development; thick, fleshy inflorescences, reduced apical dominance, reduced internode elongation, stem branching pattern altered - primary shoot ‘kinked’ at each coflorescence node, reduced fertility, small siliques borne on short pedicels held vertically and close against the stem G47 AP2 (10-75) 6 G47 Const. 35S prom. P894 3994 More tol. to drought* and better recovery from drought treatment* G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to P5311 (ARSK1 dehydration prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to P5288 (CUT1 dehydration prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 More tol. to drought* P5288 (CUT1 and better recovery prom.) from drought treatment* G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Significantly greater P5287 (LTP1 tomato plant volume prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to cold (8 C.) P5284 (RBCS3 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Late flowering P5284 (RBCS3 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Decreased sens. to P9002 (RD29A ABA prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to P9002 (RD29A dehydration prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Better recovery from P9002 (RD29A drought treatment* prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Late flowering P9002 (RD29A prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Larger leaf size P9002 (RD29A prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Larger leaf size P5318 (STM prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to cold (8 C.) P5318 (STM prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 More tol. to drought* P5318 (STM and better recovery prom.) from drought treatment* G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Late flowering P5318 (STM prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Altered sugar sensing; P5318 (STM greater tol. to sucrose prom.) (determined in 9.4% sucrose) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Late flowering P5290 (SUC2 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Larger leaf size P5290 (SUC2 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Darker green leaf color P5290 (SUC2 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Thicker stem P5290 (SUC2 prom.) G47 AP2 (10-75) 6 G47 2 comp. including P3853 4532 Greater tol. to P5290 (SUC2 dehydration prom.) G47 AP2 (10-75) 6 G47 GAL4 N-term P25186 4864 Greater tol. to (Super Active), dehydration 35S G47 AP2 (10-75) 6 G47 GAL4 N-term P25186 4864 Greater tol. to drought* (Super Active), 35S G47 AP2 (10-75) 6 G47 GAL4 N-term P25186 4864 Early flowering (Super Active), 35S G47 AP2 (10-75) 6 G47 GAL4 N-term P25186 4864 Greater tol. to 300 mM (Super Active), mannitol 35S G47 AP2 (10-75) 6 G47 Point mutation, P25735 4921 Greater tol. to cold (8 C.) 35S G47 AP2 (10-75) 6 G47 Point mutation, P25732 4920 Greater tol. to 35S dehydration G47 AP2 (10-75) 6 G47 Point mutation, P25732 4920 More tol. to drought* 35S and better recovery from drought treatment* G47 AP2 (10-75) 6 G47 Domain P25182 4863 Greater tol. to cold (8 C.) swap/chimeric variant, 35S G47 AP2 (10-75) 6 G47 Domain P25182 4863 Late flowering swap/chimeric variant, 35S G47 AP2 (10-75) 6 G47 Domain P25182 4863 Altered leaf shape swap/chimeric variant, 35S G47 AP2 (10-75) 6 G47 Domain P25182 4863 Altered leaf swap/chimeric orientation; narrow variant, 35S curled leaves held in an upward orientation G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Decreased apical dominance and bushy inflorescences G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 More lignin G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Greater tol. to cold (8 C.) G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Greater tol. to dehydration G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Thicker stem G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 More tol. to drought* and better recovery from drought treatment* G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Greater tol. to glyphosate G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Late flowering G2133 AP2 (10-77) 8 G47 Const. 35S prom. P1572 4192 Altered C/N sensing: much greater tol. to low nitrogen conditions in C/N sensing assay G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater tol. to cold (8 C.) P5326 (AP1 prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Altered sugar sensing; P5288 (CUT1 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater tol. to P5284 (RBCS3 dehydration prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater tol. to cold (8 C.) P5284 (RBCS3 prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Better recovery from P9002 (RD29A drought treatment* prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater tol. to P9002 (RD29A dehydration prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater tol. to cold (8 C.) P5318 (STM prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Late flowering P5290 (SUC2 prom.) G2133 AP2 (10-77) 8 G47 2 comp. including P4361 4552 Greater biomass P5290 (SUC2 prom.) G3646 AP2 (10-77) 2042 G47 — — n/d G3645 AP2 (10-75) 2040 G47 — — n/d G3643 AP2 (13-78) 2036 G47 Const. 35S prom. P23465 4839 More tol. to drought* and better recovery from drought treatment* G3643 AP2 (13-78) 2036 G47 Const. 35S prom. P23465 4839 Greater tol. to cold (8 C.) G3647 AP2 (13-78) 2044 G47 — — n/d G3644 AP2 (52-122) 2038 G47 Const. 35S prom. P23455 4837 Thicker stem G3644 AP2 (52-122) 2038 G47 Const. 35S prom. P23455 4837 Late flowering G3644 AP2 (52-122) 2038 G47 Const. 35S prom. P23455 4837 Greater biomass G3649 AP2 (15-87) 2046 G47 Const. 35S prom. P23456 4838 Late flowering G3649 AP2 (15-87) 2046 G47 Const. 35S prom. P23456 4838 Thicker stem G3649 AP2 (15-87) 2046 G47 Const. 35S prom. P23456 4838 Decreased apical dominance; short inflorescence internodes G3649 AP2 (15-87) 2046 G47 Const. 35S prom. P23456 4838 Greater tol. to cold (8 C.) G3649 AP2 (15-87) 2046 G47 Const. 35S prom. P23456 4838 More tol. to drought* and better recovery from drought treatment* G3651 AP2 (60-130) 2050 G47 — — n/d G3650 AP2 (75-139) 2048 G47 — — n/d G2115 AP2 (47-113) 1378 G2115 Const. 35S prom. P1507 4169 Greater tol. to cold (8 C.) G2067 AP2 (40-102) 1346 G2115 — — Only three lines tested; cold tol. not observed G2294 AP2 (32-95) 1452 G2115 — — Only three lines tested; cold tol. not observed G2294 AP2 (32-95) 1452 G2115 2 comp. including P4405 4561 Significantly greater P6506 (35S prom.) soluble solids (Brix) in tomato plants G2294 AP2 (32-95) 1452 G2115 2 comp. including P4405 4561 Significantly greater P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G2294 AP2 (32-95) 1452 G2115 2 comp. including P4405 4561 Significantly greater P5287 (LTP1 lycopene in tomato prom.) plants G2294 AP2 (32-95) 1452 G2115 2 comp. including P4405 4561 Significantly greater P6506 (35S prom.) tomato plant volume G3657 AP2 (47-109) 2052 G2115 — — n/d G207 MYB- 178 G207 Const. 35S prom. P800 3975 Greater res. to (R1)R2R3 Erysiphe (6-106) G207 MYB- 178 G207 Cons. 35S prom. P800 3975 Decreased germination (R1)R2R3 on glucose medium (6-106) G207 MYB- 178 G207 Knockout not Greater susceptibility (R1)R2R3 applicable to Botrytis (6-106) G227 MYB- 198 G207 Const. 35S prom. P1245 4080 One line showed (R1)R2R3 greater res. to Erysiphe (13-113) G227 MYB- 198 G207 Const. 35S prom. P1245 4080 Early flowering (R1)R2R3 (13-113) G230 MYB- 202 G207 Const. 35S prom. P810 3979 Altered light response; (R1)R2R3 greater shade tol.; lack (13-113) of shade avoidance phenotype G242 MYB- 214 G207 Const. 35S prom. P790 3969 Altered leaf insoluble (R1)R2R3 sugars; inc. leaf (6-106) arabinose G4218 MYB- 2264 G207 — — n/d (R1)R2R3 (31-131) G4219 MYB- 2266 G207 — — n/d (R1)R2R3 (31-131) G4220 MYB- 2268 G207 Const. 35S prom. P27042 5069 Greater res. to (R1)R2R3 Erysiphe (15-115) G4221 MYB- 2270 G207 Const. 35S prom. P27043 5070 Greater res. to (R1)R2R3 Erysiphe (5-106) G4222 MYB- 2272 G207 Const. 35S prom. P27044 5071 Greater res. to (R1)R2R3 Erysiphe (5-105) G4223 MYB- 2274 G207 Const. 35S prom. P27045 5072 One line showed (R1)R2R3 greater res. to Erysiphe (11-111) G4224 MYB- 2276 G207 — — n/d (R1)R2R3 (5-105) G4225 MYB- 2278 G207 — — n/d (R1)R2R3 (39-139) G4234 MYB- 2294 G207 Const. 35S prom. P27047 5073 Greater res. to (R1)R2R3 Erysiphe (17-117) G4235 MYB- 2296 G207 — — n/d (R1)R2R3 (15-115) G4236 MYB- 2298 G207 — — n/d (R1)R2R3 (20-120) G4237 MYB- 2300 G207 — — n/d (R1)R2R3 (10-110) G4238 MYB- 2302 G207 — n/d (R1)R2R3 (11-111) G4226 MYB- 2280 G207 — — n/d (R1)R2R3 (11-111) G4227 MYB- 2282 G207 — — n/d (R1)R2R3 (20-120) G4228 MYB- 2284 G207 Const. 35S prom. P27048 5074 Greater res. to (R1)R2R3 Erysiphe (21-121) G4229 MYB- 2286 G207 Const. 35S prom. P27049 5075 Greater res. to (R1)R2R3 Erysiphe (21-121) G4230 MYB- 2288 G207 — — n/d (R1)R2R3 (11-111) G4231 MYB- 2290 G207 — — n/d (R1)R2R3 (12-112) G4232 MYB- 2292 G207 — — n/d (R1)R2R3 (11-103) G462 IAA (11-19, 376 G462 Const. 35S prom. P1238 4078 Darker green plants 70-82, 98-114, 152-185) G461 IAA (11-19, 374 G462 Const. 35S prom. P40 3807 Wild-type in 70-82, appearance in 95-111, physiological assays 148-181) (only 3 lines tested) G4340 IAA (11-19, 2354 G462 — — n/d 57-69, 80-96, 134-167) G4346 IAA (12-20, 2356 G462 — — n/d 60-72, 82-98, 136-169) G4350 IAA (11-19, 2364 G462 — — n/d 54-66, 82-98, 131-164) G4347 IAA (10-18, 2358 G462 — — n/d 52-64, 81-96, 131-165) G4348 IAA (9-18, 2360 G462 — — n/d 52-64, 80-96, 134-167) G4349 IAA (8-16, 2362 G462 — — n/d 59-71, 87-103, 141-174) G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Late flowering G481 CAAT (20-109) 10 G481 Const. 35S prom. P26891 5063 Late flowering G481 CAAT (20-109) 10 G481 Const. 35S prom. P26496 5014 Late flowering G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Late flowering P6506 (35S prom.) G481 CAAT (20-109) 10 G481 Const. 35S prom. P26496 5014 Greater tol. to 300 mM mannitol G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Diurnal fluctuation of malate levels in young leaves G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Diurnal fluctuation of P6506 (35S prom.) malate levels in young leaves G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Photosynthesis rate increased G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Photosynthesis rate P6506 (35S prom.) increased G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater starch levels at specific timepoints and conditions G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater starch levels at P6506 (35S prom.) specific timepoints and conditions G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater proline levels in sink tissues (young leaves and inflorescences) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater proline levels P6506 (35S prom.) in sink tissues (young leaves and inflorescences) G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Altered sucrose levels; elevated sucrose levels in specific times and tissues G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Altered sucrose levels; P6506 (35S prom.) elevated sucrose levels in specific times and tissues G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Higher chlorophyll level G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Higher chlorophyll P6506 (35S prom.) level G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater tol. to cold (8 C.) G481 CAAT (20-109) 10 G481 Const. 35S prom. P25893 4937 Greater tol. to cold (8 C.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to cold (8 C.) P6506 (35S prom.) G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Decreased sens. to ABA G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Decreased sens. to P6506 (35S prom.) ABA G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater seedling vigor G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater water use efficiency G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 More tol. to drought* and better recovery from drought treatment* G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Greater non- photochemical quenching of chlorophyll fluorescence (NPQ) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater non- P6506 (35S prom.) photochemical quenching of chlorophyll fluorescence (NPQ) G481 CAAT (20-109) 10 G481 Const. 35S prom. P46 3811 Early flowering G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Early flowering P6506 (35S prom.) G481 CAAT (20-109) 10 G481 Const. 35S prom. P26496 5014 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to cold (8 C.) P5319 (AS1 prom.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Altered leaf orientation P5319 (AS1 prom.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater seedling vigor P5319 (AS1 prom.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 More tol. to drought* P5311 (ARSK1 and better recovery prom.) from drought treatment* G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Altered sugar sensing; P5287 (LTP1 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to P5287 (LTP1 dehydration prom.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to cold (8 C.) P5287 (LTP1 prom.) G481 CAAT (20-109) 10 G481 Leaf-specific P25287 4887 Greater tol. to RBCS3 prom. dehydration G481 CAAT (20-109) 10 G481 Leaf-specific P25896 4938 Greater tol. to cold (8 C.) RBCS3 prom. G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Better recovery from P9002 (RD29A drought treatment* prom.) G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Late flowering P5310 (RS1 prom.) G481 CAAT (20-109) 10 G481 Vascular-specific P21522 4824 Late flowering SUC2 prom. G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Late flowering P5290 (SUC2 prom.) G481 CAAT (20-109) 10 G481 Vascular-specific P21522 4824 Greater tol. to cold (8 C.) SUC2 prom. G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to cold (8 C.) P5290 (SUC2 prom.) G481 CAAT (20-109) 10 G481 Vascular-specific P21522 4824 Greater tol. to SUC2 prom. dehydration G481 CAAT (20-109) 10 G481 2 comp. including P6812 4601 Greater tol. to P5290 (SUC2 dehydration prom.) G481 CAAT (20-109) 10 G481 Vascular-specific P21522 4824 More tol. to drought* SUC2 prom. and better recovery from drought treatment* G481 CAAT (20-109) 10 G481 Vascular-specific P21522 4824 Darker green leaf color SUC2 prom. G481 CAAT (20-109) 10 G481 Protein-GFP C P25281 4886 Greater tol. to terminal fusion, hyperosmotic stress; 35S more tol. to 9.4% sucrose or 150 mM NaCl G481 CAAT (20-109) 10 G481 Protein-CFP C P26040 4941 Greater tol. to terminal fusion, dehydration 35S G481 CAAT (20-109) 10 G481 GAL4 C-term P21146 4746 Early flowering (Super Active), 35S G481 CAAT (20-109) 10 G481 GAL4 C-term P21146 4746 Greater seedling vigor (Super Active), 35S G481 CAAT (20-109) 10 G481 GAL4 C-term P21146 4746 Greater tol. to heat (32 C.) (Super Active), 35S G481 CAAT (20-109) 10 G481 GAL4 C-term P21146 4746 Greater tol. to NaCl (Super Active), (determined with 150 mM 35S NaCl) G481 CAAT (20-109) 10 G481 2 comp. P21281 4775 Early flowering hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G481 CAAT (20-109) 10 G481 Hemagglutinin P21287 4776 Early flowering (HA) epitope N- terminal tag, 35S G481 CAAT (20-109) 10 G481 2 comp. P26263 4964 Greater seedling vigor hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G481 CAAT (20-109) 10 G481 Point mutation, P25889 4934 Greater seedling vigor, 35S without marked changes in flowering time. G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 More tol. to drought* 35S and better recovery from drought treatment* G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Greater tol. to NaCl 35S (determined with 150 mM NaCl) G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Late flowering 35S G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Early flowering 35S G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Altered leaf shape 35S G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Darker green leaf color 35S G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Greater ABA level 35S G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Greater carotenoid 35S level G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Higher chlorophyll 35S level G481 CAAT (20-109) 10 G481 Deletion variant, P21274 4772 Higher proline level 35S G481 CAAT (20-109) 10 G481 Domain P25891 4935 Early flowering swap/chimeric variant, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21159 4747 Late flowering targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21300 4779 Late flowering targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Late flowering targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21159 4747 Greater tol. to heat (32 C.) targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21300 4779 Greater tol. to heat (32 C.) targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Greater tol. to heat (32 C.) targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21159 4747 Altered leaf shape targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21300 4779 Altered leaf shape targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Altered leaf shape targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21159 4747 Darker green leaf color targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21300 4779 Darker green leaf color targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Darker green leaf color targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Greater tol. to targeted to hyperosmotic stress; conserved domain, more tol. to 9.4% 35S sucrose, 300 mM mannitol or 150 mM NaCl G481 CAAT (20-109) 10 G481 RNAi (clade) P21159 4747 Greater seedling vigor targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21300 4779 Greater seedling vigor targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi (clade) P21305 4783 Greater seedling vigor targeted to conserved domain, 35S G481 CAAT (20-109) 10 G481 RNAi Gene- P21294 4777 Greater tol. to cold (8 C.) Specific (GS), 35S G481 CAAT (20-109) 10 G481 RNAi Gene- P21294 4777 More tol. to drought* Specific (GS), 35S and better recovery from drought treatment* G481 CAAT (20-109) 10 G481 Knockout not Early flowering applicable G481 CAAT (20-109) 10 G481 Knockout not Decreased tol. to NaCl applicable (determined with 150 mM NaCl) G482 CAAT (26-115) 12 G481 2 comp. including P5072 4594 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G482 CAAT (26-115) 12 G481 Const. 35S prom. P47 3812 Early flowering G482 CAAT (26-115) 12 G481 2 comp. including P5072 4594 Early flowering P6506 (35S prom.) G482 CAAT (26-115) 12 G481 Const. 35S prom. P47 3812 Greater tol. to 300 mM mannitol G482 CAAT (26-115) 12 G481 2 comp. including P5072 4594 Greater tol. to 300 nM P6506 (35S prom.) mannitol G482 CAAT (26-115) 12 G481 Const. 35S prom. P47 3812 Greater tol. to heat (32 C.) G482 CAAT (26-115) 12 G481 2 comp. including P5072 4594 Greater tol. to heat (32 C.) P6506 (35S prom.) G482 CAAT (26-115) 12 G481 2 comp. including P5072 4594 Early flowering P5290 (SUC2 prom.) G482 CAAT (26-115) 12 G481 Protein-CFP C- P26041 4942 Early flowering terminal fusion, 35S G482 CAAT (26-115) 12 G481 Knockout not More tol. to drought* applicable and better recovery from drought treatment* G482 CAAT (26-115) 12 G481 Knockout not Late flowering applicable G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater tol. to cold (8 C.) P6506 (35S prom.) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater tol. to P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G485 CAAT (20-109) 394 G481 Const. 35S prom. P1441 4145 More tol. to drought* and better recovery from drought treatment* G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Less sens. to ABA P6506 (35S prom.) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater tol. to NaCl P6506 (35S prom.) (determined with 150 mM NaCl) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Altered sugar sensing; P6506 (35S prom.) greater tol. to sucrose (determined in 9.4% sucrose) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Early flowering P6506 (35S prom.) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater tol. to cold (8 C.) P5319 (AS1 prom.) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater tol. to P5319 (AS1 dehydration prom.) G485 CAAT (20-109) 394 G481 2 comp. including P4190 4541 Greater seedling vigor P5319 (AS1 prom.) G485 CAAT (20-109) 394 G481 Protein-GFP C P26044 4944 Greater tol. to cold (8 C.) terminal fusion, 35S G485 CAAT (20-109) 394 G481 Protein-GFP C P26044 4944 Greater tol. to terminal fusion, dehydration 35S G485 CAAT (20-109) 394 G481 Domain P25892 4936 Late flowering swap/chimeric variant, 35S G485 CAAT (20-109) 394 G481 Domain P25892 4936 Darker green leaf color swap/chimeric variant, 35S G485 CAAT (20-109) 394 G481 Knockout not More tol. to drought* applicable and better recovery from drought treatment* G485 CAAT (20-109) 394 G481 Knockout not Less sens. to ABA applicable G485 CAAT (20-109) 394 G481 Knockout not Greater tol. to NaCl applicable (determined with 150 mM NaCl) G485 CAAT (20-109) 394 G481 Knockout not Late flowering applicable G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Better recovery from P6506 (35S prom.) drought treatment* G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Late flowering P6506 (35S prom.) G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Greater tol. to 300 mM P5284 (RBCS3 mannitol prom.) G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Greater tol. to cold (8 C.) P5284 (RBCS3 prom.) G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Greater tol. to cold (8 C.) P9002 (RD29A prom.) G1364 CAAT (29-118) 952 G481 2 comp. including P4357 4550 Greater tol. to NaCl P9002 (RD29A (determined with 150 mM prom.) NaCl) G1364 CAAT (29-118) 952 G481 Protein-CFP C- P26108 4953 More tol. to drought* terminal fusion, and better recovery 35S from drought treatment* G1364 CAAT (29-118) 952 G481 Protein-CFP C- P26108 4953 Late flowering terminal fusion, 35S G1364 CAAT (29-118) 952 G481 Protein-CFP C- P26108 4953 Darker green leaf color terminal fusion, 35S G2345 CAAT (28-117) 1476 G481 2 comp. including P8079 4607 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G2345 CAAT (28-117) 1476 G481 2 comp. including P8079 4607 Greater tol. to cold (8 C.) P6506 (35S prom.) G3470 CAAT (27-116) 1922 G481 GAL4 C-term P26500 5018 Early flowering (Super Active), 35S G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21341 4792 Greater tol. to cold (8 C.) G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21341 4792 More tol. to drought* and better recovery from drought treatment* G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21341 4792 Late flowering G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21341 4792 Greater tol. to dehydration G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Less sens. to ABA G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21341 4792 Darker green leaf color G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Darker green leaf color G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Greater tol. to hyperosmotic stress; more tol to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Greater seedling vigor G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Greater seedling vigor G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Greater tol. to NaCl (determined with 150 mM NaCl) G3470 CAAT (27-116) 1922 G481 Const. 35S prom. P21471 4818 Greater tol. to NaCl (determined with 150 mM NaCl) G3471 CAAT (26-115) 1924 G481 Const. 35S prom. P21342 4793 More tol. to drought* and better recovery from drought treatment* G3471 CAAT (26-115) 1924 G481 Const. 35S prom. P21342 4793 Darker green leaf color G3471 CAAT (26-115) 1924 G481 Const. 35S prom. P21342 4793 Late flowering G3472 CAAT (25-114) 1926 G481 Const. 35S prom. P21348 4797 More root hair G3472 CAAT (25-114) 1926 G481 Const. 35S prom. P21348 4797 Greater tol. to NaCl (determined with 150 mM NaCl) G3474 CAAT (25-114) 1930 G481 Const. 35S prom. P21344 4794 Early flowering G3474 CAAT (25-114) 1930 G481 Const. 35S prom. P21469 4817 Early flowering G3475 CAAT (23-112) 1932 G481 Const. 35S prom. P21347 4796 Early flowering G3475 CAAT (23-112) 1932 G481 Const. 35S prom. P21347 4796 Greater tol. to cold (8 C.) G3476 CAAT (26-115) 1934 G481 Const. 35S prom. P21345 4795 Greater tol. to cold (8 C.) G3476 CAAT (26-115) 1934 G481 Const. 35S prom. P21345 4795 More tol. to drought* and better recovery from drought treatment* G3476 CAAT (26-115) 1934 G481 Const. 35S prom. P21345 4795 Greater tol. to dehydration G3476 CAAT (26-115) 1934 G481 Const. 35S prom. P21345 4795 Early flowering G3478 CAAT (23-112) 1936 G481 Const. 35S prom. P21350 4798 Early flowering G3873 CAAT (29-118) 2184 G481 Const. 35S prom. P25777 4932 Late flowering G3874 CAAT (25-114) 2186 G481 Const. 35S prom. P25778 4933 Early flowering G3874 CAAT (25-114) 2186 G481 Const. 35S prom. P25778 4933 Greater seedling vigor G3875 CAAT (25-114) 2188 G481 Const. 35S prom. P26609 5042 Altered flowering time; some lines flowered early, others late G3875 CAAT (25-114) 2188 G481 Const. 35S prom. P26609 5042 Greater tol. to cold (8 C.) G3875 CAAT (25-114) 2188 G481 Const. 35S prom. P26609 5042 Darker green leaf color G3473 CAAT (23-113) 1928 G481 — — n/d G3394 CAAT (38-126) 1860 G481 Const. 35S prom. P23384 4830 Late flowering G3394 CAAT (38-126) 1860 G481 Const. 35S prom. P23481 4840 Late flowering G3394 CAAT (38-126) 1860 G481 Const. 35S prom. P21248 4756 Early flowering G3395 CAAT (19-108) 1862 G481 Const. 35S prom. P21253 4759 Altered flowering time; some lines flowered early, others late G3395 CAAT (19-108) 1862 G481 Const. 35S prom. P21253 4759 More tol. to drought* and better recovery from drought treatment* G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Greater tol. to cold (8 C.) G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Late flowering G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Less sens. to ABA G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Larger leaf size G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Altered leaf shape G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 Darker green leaf color G3396 CAAT (21-110) 1864 G481 Const. 35S prom. P23304 4826 More tol. to drought* and better recovery from drought treatment* G3396 CAAT (21-110) 1864 G481 GAL4 C-term P26499 5017 Early flowering (Super Active), 35S G3397 CAAT (23-112) 1866 G481 Const. 35S prom. P21265 4766 Early flowering G3397 CAAT (23-112) 1866 G481 Const. 35S prom. P21265 4766 Greater tol. to cold (8 C.) G3397 CAAT (23-112) 1866 G481 Const. 35S prom. P21265 4766 Greater seedling vigor G3398 CAAT (21-110) 1868 G481 Const. 35S prom. P21252 4758 Early flowering G3398 CAAT (21-110) 1868 G481 Const. 35S prom. P21252 4758 More tol. to drought* and better recovery from drought treatment* G3429 CAAT (40-124) 1880 G481 Const. 35S prom. P21251 4757 Late flowering G3429 CAAT (40-124) 1880 G481 Const. 35S prom. P21251 4757 Greater tol. to NaCl (determined with 150 mM NaCl) G3434 CAAT (18-107) 1886 G481 Const. 35S prom. P21466 4815 Greater tol. to dehydration G3434 CAAT (18-107) 1886 G481 Const. 35S prom. P21466 4815 Early flowering G3434 CAAT (18-107) 1886 G481 Const. 35S prom. P21466 4815 Greater tol. to NaCl (determined with 150 mM NaCl) G3434 CAAT (18-107) 1886 G481 Const. 35S prom. P21466 4815 More tol. to drought* and better recovery from drought treatment* G3434 CAAT (18-107) 1886 G481 Const. 35S prom. P21466 4815 Greater tol. to hyperosmotic stress; more tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G3434 CAAT (18-107) 1886 G481 Point mutation, P26921 5064 Greater biomass 35S G3434 CAAT (18-107) 1886 G481 Point mutation, P26921 5064 Late flowering 35S G3434 CAAT (18-107) 1886 G481 Point mutation, P26922 5065 Early flowering 35S G3435 CAAT (22-111) 1888 G481 Const. 35S prom. P21314 4784 More tol. to drought* and better recovery from drought treatment* G3435 CAAT (22-111) 1888 G481 Const. 35S prom. P21314 4784 Early flowering G3436 CAAT (20-109) 1890 G481 Const. 35S prom. P21381 4805 Early flowering G3436 CAAT (20-109) 1890 G481 Const. 35S prom. P21315 4785 Early flowering G3436 CAAT (20-109) 1890 G481 Const. 35S prom. P21381 4805 Greater tol. to heat (32 C.) G3436 CAAT (20-109) 1890 G481 Const. 35S prom. P21315 4785 Greater tol. to heat (32 C.) G3866 CAAT (30-126) 2182 G481 Const. 35S prom. P26548 5020 Late flowering G3866 CAAT (30-126) 2182 G481 Const. 35S prom. P26548 5020 Darker green leaf color G3866 CAAT (30-126) 2182 G481 Const. 35S prom. P26548 5020 Greater seedling vigor G3866 CAAT (30-126) 2182 G481 GAL4 C-term P26587 5025 Early flowering (Super Active), 35S G3866 CAAT (30-126) 2182 G481 GAL4 C-term P26587 5025 Greater tol. to (Super Active), dehydration 35S G3866 CAAT (30-126) 2182 G481 Point mutation, P26888 5060 Altered flowering time; 35S some lines flowered early, others flowered late G3866 CAAT (30-126) 2182 G481 Point mutation, P26889 5061 Altered flowering time; 35S some lines flowered early, others flowered late G3866 CAAT (30-126) 2182 G481 Point mutation, P26890 5062 Altered flowering time; 35S some lines flowered early, others flowered late G3866 CAAT (30-126) 2182 G481 Point mutation, P26888 5060 Darker green leaf color 35S G3866 CAAT (30-126) 2182 G481 Point mutation, P26889 5061 Darker green leaf color 35S G3866 CAAT (30-126) 2182 G481 Point mutation, P26890 5062 Darker green leaf color 35S G3866 CAAT (30-126) 2182 G481 Point mutation, P27228 5081 Darker green leaf color 35S G3866 CAAT (30-126) 2182 G481 Point mutation, P27229 5082 Darker green leaf color 35S G3876 CAAT (30-119) 2190 G481 Const. 35S prom. P25657 4913 Greater tol. to cold (8 C.) G3876 CAAT (30-119) 2190 G481 Const. 35S prom. P25657 4913 Greater tol. to dehydration G3876 CAAT (30-119) 2190 G481 Const. 35S prom. P25657 4913 More tol. to drought* and better recovery from drought treatment* G3437 CAAT (54-143) 1892 G481 — — n/d G4272 CAAT (22-118) 2338 G481 — — n/d G4276 CAAT (19-108) 2344 G481 — — n/d G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P15494 4713 More tol. to drought* and better recovery from drought treatment* G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P26883 5056 More tol. to drought* and better recovery from drought treatment* G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P15494 4713 Late flowering G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P26883 5056 Late flowering G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P15494 4713 Darker green leaf color G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P26883 5056 Darker green leaf color G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P26883 5056 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G2632 CAAT (166-223) 1614 G2632 Const. 35S prom. P15494 4713 Inc. sens. to cold (8 C.) G2632 CAAT (166-223) 1614 G2632 Protein-YFP C- P26053 4946 Darker green leaf color terminal fusion, 35S G2632 CAAT (166-223) 1614 G2632 Protein-YFP C- P26053 4946 Late flowering terminal fusion, 35S G926 CAAT (172-229) 692 G2632 Const. 35S prom. P26801 5053 Early flowering G926 CAAT (172-229) 692 G2632 Const. 35S prom. P398 3923 More tol. to drought* G926 CAAT (172-229) 692 G2632 Protein-YFP C- P26217 4958 More tol. to drought* terminal fusion, and better recovery 35S from drought treatment* G926 CAAT (172-229) 692 G2632 Protein-YFP C- P26217 4958 Darker green leaf color terminal fusion, 35S G926 CAAT (172-229) 692 G2632 RNAi (clade) P26887 5059 More tol. to drought* targeted to and better recovery conserved domain, from drought 35S treatment* G926 CAAT (172-229) 692 G2632 RNAi (clade) P26885 5057 Altered C/N sensing: targeted to greater tol. to low conserved domain, nitrogen conditions in 35S C/N sensing assay G926 CAAT (172-229) 692 G2632 RNAi (clade) P26886 5058 Altered C/N sensing: targeted to greater tol. to low conserved domain, nitrogen conditions in 35S C/N sensing assay G926 CAAT (172-229) 692 G2632 Knockout not Greater tol. to cold (8 C.) applicable G926 CAAT (172-229) 692 G2632 Knockout not Greater tol. to applicable hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G926 CAAT (172-229) 692 G2632 Knockout not More tol. to drought* applicable and better recovery from drought treatment* G926 CAAT (172-229) 692 G2632 Knockout not Greater seedling vigor applicable G926 CAAT (172-229) 692 G2632 Knockout not Less sens. to ABA applicable G3924 CAAT (163-222) 2226 G2632 Const. 35S prom. P26602 5036 Darker green leaf color G3924 CAAT (163-222) 2226 G2632 Const. 35S prom. P26602 5036 More tol. to drought* and better recovery from drought treatment* G3924 CAAT (163-222) 2226 G2632 Const. 35S prom. P26602 5036 Darker green leaf color G3924 CAAT (163-222) 2226 G2632 Const. 35S prom. P26602 5036 Glossy leaves G4261 CAAT (175-234) 2320 G2632 — — n/d G928 CAAT (179-238) 696 G928 Const. 35S prom. P143 3842 Greater tol. to cold (8 C.) G928 CAAT (179-238) 696 G928 Const. 35S prom. P143 3842 Better recovery from drought treatment* G928 CAAT (179-238) 696 G928 Const. 35S prom. P143 3842 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G928 CAAT (179-238) 696 G928 Protein-YFP C P26223 4960 Late flowering terminal fusion, 35S G928 CAAT (179-238) 696 G928 Protein-YFP C P26223 4960 Darker green leaf color terminal fusion, 35S G928 CAAT (179-238) 696 G928 Protein-YFP C P26223 4960 Greater seedling vigor terminal fusion, 35S G931 CAAT (172-231) 700 G928 Protein-YFP C- P26230 4961 Darker green leaf color terminal fusion, 35S G931 CAAT (172-231) 700 G928 Const. 35S prom. P1608 4204 Greater biomass G3926 CAAT (164-222) 2230 G928 Const. 35S prom. P26600 5035 Darker green leaf color G3926 CAAT (164-222) 2230 G928 Const. 35S prom. P26600 5035 Greater tol. to cold (8 C.) G3926 CAAT (164-222) 2230 G928 Const. 35S prom. P26600 5035 Long petiole G3926 CAAT (164-222) 2230 G928 Const. 35S prom. P26600 5035 Altered leaf orientation G3926 CAAT (164-222) 2230 G928 Const. 35S prom. P26600 5035 Greater seedling vigor G3921 CAAT (148-207) 2224 G928 — — n/d G4264 CAAT (155-214) 2326 G928 Const. 35S prom. P26593 5029 Greater tol. to cold (8 C.) G4264 CAAT (155-214) 2326 G928 Const. 35S prom. P26593 5029 Greater tol. to dehydration G4264 CAAT (155-214) 2326 G928 Const. 35S prom. P26593 5029 Greater seedling vigor G4264 CAAT (155-214) 2326 G928 Const. 35S prom. P26593 5029 Late flowering G4264 CAAT (155-214) 2326 G928 Const. 35S prom P26593 5029 Greater biomass G4264 CAAT (155-214) 2326 G928 Const. 35S prom. P26593 5029 Greater biomass G4265 CAAT (149-208) 2328 G928 — — n/d G4269 CAAT (103-162) 2334 G928 — — n/d G634 TH (62-147, 506 G634 Const. 35S prom. P1717 4237 More root mass 189-245) G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 More root mass 189-245) G634 TH (62-147, 506 G634 Const. 35S prom. P324 3895 Early flowering 189-245) G634 TH (62-147, 506 G634 Const. 35S prom. P1717 4237 Early flowering 189-245) G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 Early flowering 189-245) G634 TH (62-147, 506 G634 Const. 35S prom. P324 3895 More tol. to 189-245) dehydration G634 TH (62-147, 506 G634 Const. 35S prom. P1717 4237 More tol. to 189-245) dehydration G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 More tol. to 189-245) dehydration G634 TH (62-147, 506 G634 Const. 35S prom. P324 3895 More tol. to drought* 189-245) and better recovery from drought treatment* G634 TH (62-147, 506 G634 Const. 35S prom. P1717 4237 More tol. to drought* 189-245) and better recovery from drought treatment* G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 More tol. to drought* 189-245) and better recovery from drought treatment* G634 TH (62-147, 506 G634 Const. 35S prom. P324 3895 Greater trichome 189-245) density and size G634 TH (62-147, 506 G634 Const. 35S prom. P1717 4237 Greater trichome 189-245) density and size G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 Greater trichome 189-245) density and size G634 TH (62-147, 506 G634 Const. 35S prom. P1374 4125 Altered light response; 189-245) greater shade tol.; lack of shade avoidance phenotype G636 TH (55-145, 510 G636 Const. 35S prom. P865 3989 More tol. to drought* 405-498) and better recovery from drought treatment* G636 TH (55-145, 510 G636 Const. 35S prom. P865 3989 Greater trichome size 405-498) and density G636 TH (55-145, 510 G636 Const. 35S prom. P865 3989 Late flowering 405-498) G636 TH (55-145, 510 G636 Const. 35S prom. P865 3989 Darker green leaf color 405-498) G636 TH (55-145, 510 G636 Const. 35S prom. P865 3989 Premature senescence 405-498) G3917 TH (192-282, 2220 G636 — — n/d 508-601) G489 CAAT (68-164) 398 G489 Const. 35S prom. P51 3816 Greater tol. to cold (8 C.) G489 CAAT (68-164) 398 G489 Const. 35S prom. P51 3816 Inc. tol. to hyperosmotic stress; more root growth in 150 mM NaCl or 300 mM mannitol G489 CAAT (68-164) 398 G489 2 comp. including P3404 4510 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G489 CAAT (68-164) 398 G489 Const. 35S prom. P51 3816 More tol. to dehydration G489 CAAT (68-164) 398 G489 2 comp. including P3404 4510 More tol. to P6506 (35S prom.) dehydration G489 CAAT (68-164) 398 G489 Protein-YFP C- P26060 4949 Late flowering terminal fusion, 35S G489 CAAT (68-164) 398 G489 Protein-YFP C- P26060 4949 Larger leaf size terminal fusion, 35S G714 CAAT (58-150) 554 G489 Const. 35S prom. P111 3833 Late flowering G714 CAAT (58-150) 554 G489 Const. 35S prom. P111 3833 Inc. biomass, larger leaf size G714 CAAT (58-150) 554 G489 Protein-YFP C- P26061 4950 Late flowering terminal fusion, 35S G714 CAAT (58-150) 554 G489 Protein-YFP C- P26061 4950 Inc. biomass, larger terminal fusion, leaf size 35S G3547 CAAT (89-185) 2016 G489 — — n/d G3549 CAAT (93-189) 2020 G489 — — n/d G3550 CAAT (94-190) 2022 G489 Const. 35S prom. P26606 5039 Greater seedling vigor G3896 CAAT (89-185) 2208 G489 — — n/d G3542 CAAT (93-189) 2004 G489 Const. 35S prom. P26604 5038 Greater seedling vigor G3542 CAAT (93-189) 2004 G489 Const. 35S prom. P26604 5038 Darker green leaf color G3542 CAAT (93-189) 2004 G489 Const. 35S prom. P26604 5038 Late flowering G3544 CAAT (89-185) 2008 G489 Const. 35S prom. P26599 5034 Late flowering G3544 CAAT (89-185) 2008 G489 Const. 35S prom. P26599 5034 Darker green leaf color G3544 CAAT (89-185) 2008 G489 Const. 35S prom. P26599 5034 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G3545 CAAT (89-189) 2010 G489 — — n/d G3553 CAAT (62-158) 2028 G489 — — n/d G3554 CAAT (90-186) 2030 G489 — — n/d G3555 CAAT (54-150) 2032 G489 — — n/d G3894 CAAT (90-186) 2206 G489 Const. 35S prom. P26611 5044 Early flowering G3892 CAAT (62-158) 2202 G489 — — n/d G3893 CAAT (88-184) 2204 G489 — — n/d G3551 CAAT (87-187) 2024 G489 — — n/d G3552 CAAT (87-183) 2026 G489 Const. 35S prom. P26595 5030 Greater seedling vigor G3552 CAAT (87-183) 2026 G489 Const. 35S prom. P26595 5030 Greater tol. to cold (8 C.) G3552 CAAT (87-183) 2026 G489 Const. 35S prom. P26595 5030 Darker green leaf color G3552 CAAT (87-183) 2026 G489 Const. 35S prom. P26595 5030 Late flowering G4256 CAAT (84-180) 2310 G489 — — n/d G4257 CAAT (90-186) 2312 G489 — — n/d G1782 CAAT (178-237) 1162 G1782 Const. 35S prom. P966 4010 Greater biomass G1782 CAAT (178-237) 1162 G1782 Const. 35S prom. P966 4010 Darker green leaf color G1363 CAAT (171-230) 950 G1782 Const. 35S prom. P724 3956 Early flowering G1363 CAAT (171-230) 950 G1782 Const. 35S prom. P724 3956 Darker green leaf color G1363 CAAT (171-230) 950 G1782 Const. 35S prom. P724 3956 Greater resistance to Fusarium G1363 CAAT (171-230) 950 G1782 Protein-YFP C- P26121 4954 Late flowering terminal fusion, 35S G1363 CAAT (171-230) 950 G1782 Protein-YFP C- P26121 4954 Larger leaf size terminal fusion, 35S G1363 CAAT (171-230) 950 G1782 Protein-YFP C- P26121 4954 Darker green leaf color terminal fusion, 35S G3920 CAAT (149-208) 2222 G1782 Const. 35S prom. P26608 5041 More tol. to drought* and better recovery from drought treatment* G3920 CAAT (149-208) 2222 G1782 Const. 35S prom. P26608 5041 Greater seedling vigor G3925 CAAT (138-197) 2228 G1782 Const. 35S prom. P26597 5032 Darker green leaf color G3925 CAAT (138-197) 2228 G1782 Const. 35S prom. P26597 5032 Late flowering G4262 CAAT (142-201) 2322 G1782 — — n/d G4263 CAAT (137-196) 2324 G1782 — — n/d G4270 CAAT (131-191) 2336 G1782 — — n/d G3546 CAAT (78-175) 2012 G3546 Const. 35S prom. P26603 5037 Greater seedling vigor G3546 CAAT (78-175) 2012 G3546 Const. 35S prom. P26603 5037 Greater tol. to cold (8 C.) G3546 CAAT (78-175) 2012 G3546 Const. 35S prom. P26603 5037 Late flowering G3546 CAAT (78-175) 2012 G3546 Const. 35S prom. P26603 5037 Darker green leaf color G3546 CAAT (78-175) 2012 G3546 Const. 35S prom. P26603 5037 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G3911 CAAT (70-167) 2218 G3546 Const. 35S prom. P26591 5028 Greater tol. to cold (8 C.) G3911 CAAT (70-167) 2218 G3546 Const. 35S prom. P26591 5028 Greater seedling vigor G3909 CAAT (73-170) 2216 G3546 Const. 35S prom. P26596 5031 Greater seedling vigor G3909 CAAT (73-170) 2216 G3546 Const. 35S prom. P26596 5031 Late flowering G3909 CAAT (73-170) 2216 G3546 Const. 35S prom. P26596 5031 Darker green leaf color G3909 CAAT (73-170) 2216 G3546 Const. 35S prom. P26596 5031 Greater biomass G4258 CAAT (70-167) 2316 G3546 — — n/d G1334 CAAT (133-190) 936 G1334 Const. 35S prom. P714 3953 Darker green leaf color G1334 CAAT (133-190) 936 G1334 Const. 35S prom. P714 3953 Early flowering G1334 CAAT (133-190) 936 G1334 Const. 35S prom. P714 3953 Greater seedling vigor G1334 CAAT (133-190) 936 G1334 Const. 35S prom. P714 3953 Greater biomass G1334 CAAT (133-190) 936 G1334 Protein-YFP C- P26238 4962 Darker green leaf color terminal fusion, 35S G1334 CAAT (133-190) 936 G1334 Protein-YFP C- P26238 4962 Leaf orientation terminal fusion, 35S G927 CAAT (136-199) 694 G1334 Protein-YFP C- P26197 4957 Late flowering terminal fusion, 35S G927 CAAT (136-199) 694 G1334 Protein-YFP C- P26197 4957 Darker green leaf color terminal fusion, 35S G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 More tol. to P6506 (35S prom.) hyperosmotic stress; better germination in 9.4% sucrose, 300 mM mannitol, or 150 mM NaCl G1820 CAAT (70-133) 1200 G1820 Const. 35S prom. P1284 4097 Greater tol. to germination in cold (8 C.) G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 Greater tol. to P6506 (35S prom.) germination in cold (8 C.) G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 Early flowering P6506 (35S prom.) G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 Less sens. to ABA P6506 (35S prom.) G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 Inc. seed protein P6506 (35S prom.) content G1820 CAAT (70-133) 1200 G1820 2 comp. including P3372 4506 Decreased seed oil P6506 (35S prom.) content G1820 CAAT (70-133) 1200 G1820 Const. 35S prom. P1284 4097 More tol. to drought* and better recovery from drought treatment* G1820 CAAT (70-133) 1200 G1820 Protein-YFP C- P26064 4951 Darker green leaf color terminal fusion, 35S G1836 CAAT (24-110) 1212 G1836 2 comp. including P3603 4518 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G1836 CAAT (24-110) 1212 G1836 2 comp. including P3603 4518 Greater tol. to P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G1836 CAAT (24-110) 1212 G1836 2 comp. including P3603 4518 Less sens. to ABA P6506 (35S prom.) G1836 CAAT (24-110) 1212 G1836 2 comp. including P3603 4518 Greater tol. to cold (8 C.) P6506 (35S prom.) G1836 CAAT (24-110) 1212 G1836 2 comp. including P3603 4518 Late flowering P6506 (35S prom.) G1836 CAAT (24-110) 1212 G1836 Protein-YFP C- P26052 4945 Late flowering terminal fusion, 35S G1836 CAAT (24-110) 1212 G1836 Protein-YFP C- P26052 4945 Darker green leaf color terminal fusion, 35S G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Decreased apical dominance, more secondary meristems in the rosette G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Altered leaf shape G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Color: light green G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Leaf orientation G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Greater biomass G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Greater tol. to dehydration G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Greater seed protein content G1818 CAAT (24-116) 1196 G1836 Const. 35S prom. P1677 4219 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1818 CAAT (24-116) 1196 G1836 Protein-YFP C- P26159 4956 Color: light green terminal fusion, 35S G1818 CAAT (24-116) 1196 G1836 Protein-YFP C- P26159 4956 Late flowering terminal fusion, 35S G1818 CAAT (24-116) 1196 G1836 Protein-YFP C- P26159 4956 Greater biomass terminal fusion, 35S G3969 CAAT (36-125) 2244 G3969 Const. 35S prom. P26612 5045 Greater seedling vigor G3969 CAAT (36-125) 2244 G3969 Const. 35S prom. P26612 5045 Greater tol. to cold (8 C.) G3969 CAAT (36-125) 2244 G3969 Const. 35S prom. P26612 5045 Late flowering G3969 CAAT (36-125) 2244 G3969 Const. 35S prom. P26612 5045 Darker green leaf color G929 CAAT (98-157) 698 G929 Const. 35S prom. P399 3924 More tol. to drought* and better recovery from drought treatment* G929 CAAT (98-157) 698 G929 Const. 35S prom. P399 3924 Late flowering G929 CAAT (98-157) 698 G929 Const. 35S prom. P399 3924 Greater biomass G929 CAAT (98-157) 698 G929 Const. 35S prom. P399 3924 Altered leaf shape G929 CAAT (98-157) 698 G929 Const. 35S prom. P399 3924 Darker green leaf color G929 CAAT (98-157) 698 G929 Protein-YFP C P26219 4959 Late flowering terminal fusion, 35S G929 CAAT (98-157) 698 G929 Protein-YFP C P26219 4959 Darker green leaf color terminal fusion, 35S G2344 CAAT (100-159) 1474 G929 Const. 35S prom. P1627 4212 Darker green leaf color G4267 CAAT (110-169) 2330 G929 — — n/d G4267 CAAT (110-169) 2332 G929 — — n/d G483 CAAT (64-160) 390 G483 Const. 35S prom. P48 3813 Better recovery from drought treatment* G3548 CAAT (77-173) 2018 G483 Const. 35S prom. P26610 5043 Darker green leaf color G3548 CAAT (77-173) 2018 G483 Const. 35S prom. P26610 5043 Greater seedling vigor G3548 CAAT (77-173) 2018 G483 Const. 35S prom. P26610 5043 Late flowering G3899 CAAT (89-185) 2210 G483 — — n/d G3900 CAAT (70-166) 2212 G483 — — n/d G3907 CAAT (92-184) 2214 G483 — — n/d G1248 CAAT (43-126) 878 G1248 Const. 35S prom. P1446 4146 More tol. to drought* and better recovery from drought treatment* G1248 CAAT (43-126) 878 G1248 Const. 35S prom. P1446 4146 Greater tol. to cold (8 C.) G1248 CAAT (43-126) 878 G1248 Const. 35S prom. P1446 4146 Early flowering G1248 CAAT (43-126) 878 G1248 Const. 35S prom. P1446 4146 Darker green leaf color G3837 CAAT (34-123) 2152 G1248 — — n/d G3835 CAAT (3-92) 2150 G1248 — — n/d G3931 CAAT (23-111) 2234 G1248 — — n/d G4273 CAAT (28-117) 2340 G1248 — — n/d G620 CAAT (28-117) 494 G620 Const. 35S prom. P321 3894 Greater tol. to cold (8 C.) G1821 CAAT (57-146) 1202 G620 Const. 35S prom. P26819 5054 Greater tol. to cold (8 C.) G1821 CAAT (57-146) 1202 G620 Const. 35S prom. P1286 4099 Late flowering G1821 CAAT (57-146) 1202 G620 Const. 35S prom. P26819 5054 Early flowering G1821 CAAT (57-146) 1202 G620 Const. 35S prom. P26819 5054 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G1821 CAAT (57-146) 1202 G620 Protein-CFP C- P26037 4939 Greater tol. to terminal fusion, dehydration 35S G3939 CAAT (31-120) 2238 G620 — — G3937 CAAT (35-124) 2236 G620 — — n/d G3839 CAAT (48-137) 2156 G620 — — n/d G3074 CAAT (3-86) 1826 G3074 Const. 35S prom. P2712 4452 Greater tol. to dehydration G3074 CAAT (3-86) 1826 G3074 Const. 35S prom. P2712 4452 Better recovery from drought treatment* G4253 CAAT (10-86) 2304 G3074 — — n/d G4254 CAAT (10-86) 2306 G3074 — — n/d G4255 CAAT (10-86) 2308 G3074 — — n/d G1781 CAAT (35-124) 1160 G1781 Const. 35S prom. P965 4009 Early flowering G1781 CAAT (35-124) 1160 G1781 Const. 35S prom. P965 4009 Better recovery from drought treatment* G1781 CAAT (35-124) 1160 G1781 Protein-CFP C- P26043 4943 Late flowering terminal fusion, 35S G1819 CAAT (52-148) 1198 G1819 Const. 35S prom. P1285 4098 Early flowering G1819 CAAT (52-148) 1198 G1819 Protein-YFP C- P26065 4952 Late flowering terminal fusion, 35S G1819 CAAT (52-148) 1198 G1819 Const. 35S prom. P1285 4098 Altered leaf shape G1819 CAAT (52-148) 1198 G1819 Const. 35S prom. P1285 4098 Light green color G1819 CAAT (52-148) 1198 G1819 Protein-YFP C- P26065 4952 Darker green leaf color terminal fusion, 35S G1646 CAAT (66-162) 1100 G1646 Const. 35S prom. P964 4008 Greater biomass G1646 CAAT (66-162) 1100 G1646 Const. 35S prom. P964 4008 More seed oil content G1646 CAAT (66-162) 1100 G1646 Protein-YFP C- P26130 4955 Altered leaf terminal fusion, orientation, upright 35S leaves, slightly longer petioles G1646 CAAT (66-162) 1100 G1646 Protein-YFP C- P26130 4955 Altered leaf shape terminal fusion, 35S G715 CAAT (53-149) 556 G1646 Const. 35S prom. P15502 4716 More seed oil content G715 CAAT (53-149) 556 G1646 Protein-YFP C- P26057 4947 Altered leaf terminal fusion, orientation, upright 35S leaves G715 CAAT (53-149) 556 G1646 Protein-YFP C- P26057 4947 Greater biomass terminal fusion, 35S G3886 CAAT (59-155) 2198 G1646 Const. 35S prom. P26607 5040 Early flowering G3886 CAAT (59-155) 2198 G1646 Const. 35S prom. P26607 5040 Greater tol. to cold (8 C.) G3883 CAAT (54-150) 2192 G1646 Const. 35S prom. P26821 5055 Greater tol. to cold (8 C.) G3883 CAAT (54-150) 2192 G1646 Const. 35S prom. P26821 5055 Early flowering G3884 CAAT (47-143) 2194 G1646 — — n/d G3543 CAAT (55-153) 2006 G1646 Const. 35S prom. P26598 5033 Late flowering G3543 CAAT (55-153) 2006 G1646 Const. 35S prom. P26598 5033 Early flowering G3885 CAAT (54-150) 2196 G1646 — — n/d G3889 CAAT (54-152) 2200 G1646 Const. 35S prom. P26590 5027 Greater tol. to cold (8 C.) G3889 CAAT (54-152) 2200 G1646 Const. 35S prom. P26590 5027 Greater tol. to dehydration G3889 CAAT (54-152) 2200 G1646 Const. 35S prom. P26590 5027 Better recovery from drought treatment* G3889 CAAT (54-152) 2200 G1646 Const. 35S prom. P26590 5027 Early flowering G3889 CAAT (54-152) 2200 G1646 Const. 35S prom. P26590 5027 Greater seedling vigor G4259 CAAT (55-153) 2318 G1646 — — n/d G484 CAAT (11-99) 392 G484 Const. 35S prom. P49 3814 Greater tol. to dehydration G484 CAAT (11-99) 392 G484 Knockout not Altered seed applicable glucosinolate profile G2631 CAAT (11-99) 1612 G484 Const. 35S prom. P2011 4309 Greater tol. to dehydration G2631 CAAT (11-99) 1612 G484 Const. 35S prom. P2011 4309 Greater biomass G2631 CAAT (11-99) 1612 G484 Protein-CFP C- P26039 4940 Greater biomass terminal fusion, 35S G3940 CAAT (11-99) 2240 G484 — — n/d G4275 CAAT (11-99) 2342 G484 — — n/d G486 CAAT (3-66) 396 G486 Const. 35S prom. P50 3815 Darker green leaf color G486 CAAT (3-66) 396 G486 Const. 35S prom. P50 3815 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G486 CAAT (3-66) 396 G486 Const. 35S prom. P50 3815 Late flowering G486 CAAT (3-66) 396 G486 Protein-CFP C- P26277 4965 Late flowering terminal fusion, 35S G486 CAAT (3-66) 396 G486 Protein-CFP C- P26277 4965 Darker green leaf color terminal fusion, 35S G490 CAAT (56-145) 400 G486 Const. 35S prom. P912 3998 Larger leaf size, greater biomass G490 CAAT (56-145) 400 G486 Const. 35S prom. P912 3998 Early flowering G490 CAAT (56-145) 400 G486 Protein-YFP C- P26059 4948 Altered flowering time; terminal fusion, some lines flowered 35S early, others late G2539 NAC (54-178) 1562 G2539 Const. 35S prom. P13710 4633 Darker green leaf color G2539 NAC (54-178) 1562 G2539 Const. 35S prom. P13710 4633 Early flowering G1249 CAAT (13-89) 880 G1249 Const. 35S prom. P1184 4059 Early flowering G3075 CAAT (111-192) 1828 G3075 Const. 35S prom. P2797 4473 Early flowering G482 & CAAT (26-115) & 12 & G481-related Double Knockout not Late flowering G485 CAAT (20-109) 394 sequences, applicable double knockouts G5 AP2 (149-216) 38 G974 Const. 35S prom. P164 3849 Small plant G974 AP2 (80-147) 728 G974 Const. 35S prom. P1510 4171 Altered seed oil content G974 AP2 (80-147) 728 G974 Const. 35S prom. P1510 4171 Altered light response; greater shade tol.; lack of shade avoidance phenotype G583 HLH/MYC 462 G583 Knockout not Greater res. to Botrytis (445-502) applicable G664 MYB- 528 G664 Const. 35S prom. P98 3827 Better germination and (R1)R2R3 growth in cold (8 C.) (14-116) G664 MYB- 528 G664 Const. 35S prom. P98 3827 Altered light response; (R1)R2R3 greater shade tol.; lack (14-116) of shade avoidance phenotype G197 MYB- 166 G664 Const. 35S prom. P814 3980 No positive (R1)R2R3 physiological results (14-116) (only 3 lines generated) G255 MYB- 228 G664 Const. 35S prom. P787 3968 No positive (R1)R2R3 physiological results (14-116) (only 3 lines generated) G255 MYB- 228 G664 Const. 35S prom. P1277 4094 Early flowering (R1)R2R3 (14-116) G3529 MYB- 1994 G664 — — n/d (R1)R2R3 (14-116) G3527 MYB- 1990 G664 — — n/d (R1)R2R3 (13-117) G3528 MYB- 1992 G664 — — n/d (R1)R2R3 (13-117) G3503 MYB- 1960 G664 — — n/d (R1)R2R3 (14-116) G3504 MYB- 1962 G664 — — n/d (R1)R2R3 (14-116) G3505 MYB- 1964 G664 — — n/d (R1)R2R3 (14-116) G3506 MYB- 1966 G664 — — n/d (R1)R2R3 (14-116) G3507 MYB- 1968 G664 — — n/d (R1)R2R3 (14-116) G3508 MYB- 1970 G664 — — n/d (R1)R2R3 (14-116) G3509 MYB- 1972 G664 — — n/d (R1)R2R3 (14-116) G3531 MYB- 1996 G664 — — n/d (R1)R2R3 (14-116) G3532 MYB- 1998 G664 — — n/d (R1)R2R3 (14-116) G3533 MYB- 2000 G664 — — n/d (R1)R2R3 (14-116) G3534 MYB- 2002 G664 — — n/d (R1)R2R3 (14-116) G4637 MYB- 2366 G664 — — n/d (R1)R2R3 (14-116) G4638 MYB- 2368 G664 — — n/d (R1)R2R3 (14-116) G4639 MYB- 2370 G664 — — n/d (R1)R2R3 (14-116) G4640 MYB- 2372 G664 — — n/d (R1)R2R3 (76-178) G682 MYB- 550 G682 2 comp. including P5099 4595 Greater tol. to related (33-77) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G682 MYB- 550 G682 2 comp. including P108 3832 More tol. to low related (33-77) P6506 (35S prom.) nitrogen conditions G682 MYB- 550 G682 2 comp. including P5099 4595 More tol. to low related (33-77) P6506 (35S prom.) nitrogen conditions G682 MYB- 550 G682 2 comp. including P23516 4842 More tol. to low related (33-77) P6506 (35S prom.) nitrogen conditions G682 MYB- 550 G682 2 comp. including P23517 4843 More tol. to low related (33-77) P6506 (35S prom.) nitrogen conditions G682 MYB- 550 G682 2 comp. including P5099 4595 Altered C/N sensing: related (33-77) P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G682 MYB- 550 G682 2 comp. including P5099 4595 Greater tol. to related (33-77) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G682 MYB- 550 G682 2 comp. including P5099 4595 Less sens. to ABA related (33-77) P6506 (35S prom.) G682 MYB- 550 G682 Const. 35S prom. P108 3832 More root hair related (33-77) G682 MYB- 550 G682 2 comp. including P5099 4595 More root hair related (33-77) P6506 (35S prom.) G682 MYB- 550 G682 2 comp. including P5099 4595 Glabrous, lack of related (33-77) P6506 (35S prom.) trichomes G682 MYB- 550 G682 Const. 35S prom. P108 3832 Decreased anthocyanin related (33-77) G682 MYB- 550 G682 2 comp. including P5099 4595 Decreased anthocyanin related (33-77) P6506 (35S prom.) G682 MYB- 550 G682 Const. 35S prom. P108 3832 Photosynthesis rate related (33-77) reduced G682 MYB- 550 G682 2 comp. including P5099 4595 Photosynthesis rate related (33-77) P6506 (35S prom.) reduced G682 MYB- 550 G682 Const. 35S prom. P108 3832 Decreased chlorophyll related (33-77) G682 MYB- 550 G682 2 comp. including P5099 4595 Decreased chlorophyll related (33-77) P6506 (35S prom.) G682 MYB- 550 G682 2 comp. including P5099 4595 Better germination and related (33-77) P6506 (35S prom.) growth in heat (32 C.) G682 MYB- 550 G682 Const. 35S prom. P108 3832 More tol. to drought* related (33-77) and better recovery from drought treatment* G682 MYB- 550 G682 2 comp. including P5099 4595 More tol. to drought* related (33-77) P6506 (35S prom.) and better recovery from drought treatment* G682 MYB- 550 G682 2 comp. including P5099 4595 Greater tol. to NaCl related (33-77) P5288 (CUT1 (determined with 150 mM prom.) NaCl) G682 MYB- 550 G682 Epidermal-specific P23322 4827 Better recovery from related (33-77) CUT1 prom. drought treatment* G682 MYB- 550 G682 2 comp. including P5099 4595 Greater tol. to NaCl related (33-77) P5288 (CUT1 (determined with 150 mM prom.) NaCl) G682 MYB- 550 G682 Epidermal-specific P23322 4827 Decreased trichome related (33-77) CUT1 prom. density G682 MYB- 550 G682 Epidermal and P23328 4828 Greater tol. to cold (8 C.) related (33-77) vascular-specific LTP1 prom. G682 MYB- 550 G682 Epidermal and P23328 4828 Altered C/N sensing: related (33-77) vascular-specific greater tol. to low LTP1 prom. nitrogen conditions in C/N sensing assay G682 MYB- 550 G682 Epidermal and P23328 4828 More tol. to low related (33-77) vascular-specific nitrogen conditions LTP1 prom. G682 MYB- 550 G682 Epidermal and P23328 4828 Decreased trichome related (33-77) vascular-specific density LTP1 prom. G682 MYB- 550 G682 Epidermal and P23328 4828 Decreased anthocyanin related (33-77) vascular-specific LTP1 prom. G682 MYB- 550 G682 2 comp. including P5099 4595 Better recovery from related (33-77) P5284 (RBCS3 drought treatment* prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Greater tol. to cold (8 C.) related (33-77) P9002 (RD29A prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Better recovery from related (33-77) P9002 (RD29A drought treatment* prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Greater tol. to related (33-77) P5310 (RS1 dehydration prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Late flowering related (33-77) P5310 (RS1 prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Greater tol. to cold (8 C.) related (33-77) P5290 (SUC2 prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 More tol. to drought* related (33-77) P5290 (SUC2 and better recovery prom.) from drought treatment* G682 MYB- 550 G682 2 comp. including P23517 4843 Greater tol. to heat (32 C.) related (33-77) P5290 (SUC2 prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Greater biomass related (33-77) P5290 (SUC2 prom.) G682 MYB- 550 G682 2 comp. including P23517 4843 Decreased trichome related (33-77) P5290 (SUC2 density prom.) G682 MYB- 550 G682 GAL4 N-term P23482 4841 Greater tol. to related (33-77) (Super Active), dehydration 35S G682 MYB- 550 G682 GAL4 N-term P23482 4841 Greater tol. to low related (33-77) (Super Active), nitrogen conditions 35S G682 MYB- 550 G682 GAL4 C-term P21144 4744 Decreased anthocyanin related (33-77) (Super Active), 35S G682 MYB- 550 G682 GAL4 C-term P21144 4744 Greater tol. to low related (33-77) (Super Active), nitrogen conditions 35S G682 MYB- 550 G682 Protein-GFP C P25290 4888 Greater tol. to cold (8 C.) related (33-77) terminal fusion, 35S G682 MYB- 550 G682 Protein-GFP C P25290 4888 Greater tol. to low related (33-77) terminal fusion, nitrogen conditions 35S G682 MYB- 550 G682 Protein-GFP C P25290 4888 Altered sugar sensing; related (33-77) terminal fusion, greater tol. to sucrose 35S (determined in 9.4% sucrose) G682 MYB- 550 G682 Protein-GFP C P25290 4888 More tol. to drought* related (33-77) terminal fusion, and better recovery 35S from drought treatment* G682 MYB- 550 G682 RNAi (clade) P21299 4778 Less sens. to ABA related (33-77) targeted to conserved domain, 35S G682 MYB- 550 G682 RNAi (clade) P21299 4778 Better recovery from related (33-77) targeted to drought treatment* conserved domain, 35S G682 MYB- 550 G682 RNAi Gene- P21111 4742 Late flowering related (33-77) Specific (GS), 35S G682 MYB- 550 G682 RNAi Gene- P21111 4742 Greater tol. to NaCl related (33-77) Specific (GS), 35S (determined with 150 mM NaCl) G682 MYB- 550 G682 RNAi Gene- P21111 4742 Less sens. to ABA related (33-77) Specific (GS), 35S G682 MYB- 550 G682 Knockout not More tol. to drought* related (33-77) applicable and better recovery from drought treatment* G682 MYB- 550 G682 Knockout not Less sens. to ABA related (33-77) applicable G225 MYB- 194 G682 Const. 35S prom. P796 3973 Altered C/N sensing: (CPC) related (36-80) much greater tol. to low nitrogen conditions in C/N sensing assay G225 MYB- 194 G682 Const. 35S prom. P796 3973 More tol. to low (CPC) related (36-80) nitrogen conditions G225 MYB- 194 G682 Const. 35S prom. P796 3973 Greater tol. to heat (32 C.) (CPC) related (36-80) G225 MYB- 194 G682 Const. 35S prom. P796 3973 More root hairs (CPC) related (36-80) G225 MYB- 194 G682 Const. 35S prom. P796 3973 Glabrous, lack of (CPC) related (36-80) trichomes G226 MYB- 196 G682 2 comp. including P3359 4502 Less sens. to ABA related (38-82) P6506 (35S prom.) G226 MYB- 196 G682 2 comp. including P3359 4502 Greater tol. to low related (38-82) P6506 (35S prom.) nitrogen conditions G226 MYB- 196 G682 2 comp. including P3359 4502 More root hair related (38-82) P6506 (35S prom.) G226 MYB- 196 G682 2 comp. including P3359 4502 Altered C/N sensing: related (38-82) P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G226 MYB- 196 G682 2 comp. including P3359 4502 Greater tol. to related (38-82) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G226 MYB- 196 G682 2 comp. including P3359 4502 Decreased anthocyanin related (38-82) P6506 (35S prom.) G226 MYB- 196 G682 2 comp. including P3359 4502 Inc. seed protein related (38-82) P6506 (35S prom.) content G226 MYB- 196 G682 2 comp. including P3359 4502 Glabrous, lack of related (38-82) P6506 (35S prom.) trichomes G226 MYB- 196 G682 2 comp. including P3359 4502 Greater tol. to cold (8 C.) related (38-82) P5311 (ARSK1 prom.) G226 MYB- 196 G682 2 comp. including P3359 4502 Significantly greater related (38-82) P5324 (Cru prom.) tomato plant volume G1816 MYB- 1194 G682 2 comp. including P8223 4608 Altered C/N sensing: related (30-74) P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G1816 MYB- 1194 G682 2 comp. including P8223 4608 Greater tol. to low related (30-74) P6506 (35S prom.) nitrogen conditions G1816 MYB- 1194 G682 2 comp. including P8223 4608 Altered sugar sensing; related (30-74) P6506 (35S prom.) much less seedling stress in 5% glucose, more tol. to 9.4% sucrose G1816 MYB- 1194 G682 2 comp. including P8223 4608 More tol. to drought* related (30-74) P6506 (35S prom.) and better recovery from drought treatment* G1816 MYB- 1194 G682 2 comp. including P8223 4608 Ectopic root hairs, related (30-74) P6506 (35S prom.) more root hairs G1816 MYB- 1194 G682 2 comp. including P8223 4608 Glabrous leaves related (30-74) P6506 (35S prom.) G1816 MYB- 1194 G682 2 comp. including P8223 4608 Greater tol. to low related (30-74) P5288 (CUT1 nitrogen conditions prom.) G1816 MYB- 1194 G682 2 comp. including P8223 4608 Greater tol. to low related (30-74) P5290 (SUC2 nitrogen conditions prom.) G1816 MYB- 1194 G682 Protein-GFP C P25296 4889 Late flowering related (30-74) terminal fusion, 35S G1816 MYB- 1194 G682 Knockout not Greater tol. to NaCl related (30-74) applicable (determined with 150 mM NaCl) G1816 MYB- 1194 G682 Knockout not Greater trichome related (30-74) applicable density and more trichome branching G2718 MYB- 1654 G682 2 comp. including P8664 4613 Decreased anthocyanin related (32-76) P6506 (35S prom.) G2718 MYB- 1654 G682 2 comp. including P8664 4613 Altered sugar sensing; related (32-76) P6506 (35S prom.) greater tol. to sucrose (determined in 9.4% sucrose) G2718 MYB- 1654 G682 2 comp. including P8664 4613 Decreased trichome related (32-76) P6506 (35S prom.) density ranging from mild to glabrous G2718 MYB- 1654 G682 2 comp. including P8664 4613 Late flowering related (32-76) P6506 (35S prom.) G2718 MYB- 1654 G682 2 comp. including P8664 4613 Altered C/N sensing: related (32-76) P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G2718 MYB- 1654 G682 2 comp. including P8664 4613 Ectopic root hairs, related (32-76) P6506 (35S prom.) more root hairs G3930 MYB- 2232 G682 Const. 35S prom. P26589 5026 Altered C/N sensing: related (33-77) greater tol. to low nitrogen conditions in C/N sensing assay G3930 MYB- 2232 G682 Const. 35S prom. P26589 5026 Decreased trichome related (33-77) density G3930 MYB- 2232 G682 Const. 35S prom. P26589 5026 Early flowering related (33-77) G3930 MYB- 2232 G682 Const. 35S prom. P26589 5026 Greater seedling vigor related (33-77) G3445 MYB- 1896 G682 Const. 35S prom. P21352 4800 Late flowering related (25-69) G3445 MYB- 1896 G682 Const. 35S prom. P21352 4800 Less sens. to ABA related (25-69) G3445 MYB- 1896 G682 Const. 35S prom. P21352 4800 Decreased trichome related (25-69) density G3446 MYB- 1898 G682 Const. 35S prom. P21353 4801 Decreased trichome related (26-70) density G3446 MYB- 1898 G682 Const. 35S prom. P21353 4801 Better recovery from related (26-70) drought treatment* G3446 MYB- 1898 G682 Const. 35S prom. P21353 4801 Late flowering related (26-70) G3446 MYB- 1898 G682 Const. 35S prom. P21353 4801 Early flowering related (26-70) G3447 MYB- 1900 G682 Const. 35S prom. P21354 4802 More tol. to drought* related (26-70) and better recovery from drought treatment* G3447 MYB- 1900 G682 Const. 35S prom. P21354 4802 Decreased trichome related (26-70) density G3447 MYB- 1900 G682 Const. 35S prom. P21354 4802 Greater tol. to low related (26-70) nitrogen conditions G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Altered C/N sensing: related (26-70) greater tol. to low nitrogen conditions in C/N sensing assay G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Greater tol. to low related (26-70) nitrogen conditions G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 More root hair related (26-70) G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Decreased trichome related (26-70) density G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Decreased anthocyanin related (26-70) G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Greater tol. to cold (8 C.) related (26-70) G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Better recovery from related (26-70) drought treatment* G3448 MYB- 1902 G682 Const. 35S prom. P21355 4803 Color: light green related (26-70) G3449 MYB- 1904 G682 Const. 35S prom. P21356 4804 Altered C/N sensing: related (26-70) greater tol. to low nitrogen conditions in C/N sensing assay G3449 MYB- 1904 G682 Const. 35S prom. P21356 4804 Greater tol. to low related (26-70) nitrogen conditions G3449 MYB- 1904 G682 Const. 35S prom. P21356 4804 More root hair related (26-70) G3449 MYB- 1904 G682 Const. 35S prom. P21356 4804 Decreased trichome related (26-70) density G3449 MYB- 1904 G682 Const. 35S prom. P21356 4804 Greater tol. to cold (8 C.) related (26-70) G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 More tol. to drought* related (20-64) and better recovery from drought treatment* G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 Altered C/N sensing: related (20-64) greater tol. to low nitrogen conditions in C/N sensing assay G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 Greater tol. to low related (20-64) nitrogen conditions G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 More root hair related (20-64) G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 Decreased trichome related (20-64) density G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 Greater tol. to cold (8 C.) related (20-64) G3450 MYB- 1906 G682 Const. 35S prom. P21351 4799 Greater tol. to heat (32 C.) related (20-64) G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Greater tol. to cold (8 C.) related (32-76) G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Altered C/N sensing: related (32-76) greater tol. to low nitrogen conditions in C/N sensing assay G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Greater tol. to low related (32-76) nitrogen conditions G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Decreased anthocyanin related (32-76) G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 More root hair related (32-76) G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Color: Pale related (32-76) G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Decreased trichome related (32-76) density G3392 MYB- 14 G682 Const. 35S prom. P21255 4761 Greater tol. to related (32-76) hyperosmotic stress; more tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Greater tol. to cold (8 C.) related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Greater tol. to cold (8 C.) related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Altered C/N sensing: related (31-75) greater tol. to low nitrogen conditions in C/N sensing assay G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Altered C/N sensing: related (31-75) greater tol. to low nitrogen conditions in C/N sensing assay G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Greater tol. to low related (31-75) nitrogen conditions G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Greater tol. to low related (31-75) nitrogen conditions G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 More root hair related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 More root hair related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Decreased anthocyanin related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Decreased anthocyanin related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Color: Pale related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Color: Pale related (31-75) G3393 MYB- 1858 G682 Const. 35S prom. P21254 4760 Decreased trichome related (31-75) density G3393 MYB- 1858 G682 Const. 35S prom. P21256 4762 Decreased trichome related (31-75) density G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 Decreased anthocyanin related (31-75) G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 Greater tol. to low related (31-75) nitrogen conditions G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 More root hair related (31-75) G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 Color: Pale related (31-75) G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 Decreased trichome related (31-75) density G3444 MYB- 1894 G682 Const. 35S prom. P21320 4787 Better recovery from related (31-75) drought treatment* G3431 MYB- 1882 G682 Const. 35S prom. P21324 4788 Greater tol. to sucrose related (31-75) (determined in 9.4% sucrose) G3431 MYB- 1882 G682 Const. 35S prom. P21324 4788 Greater tol. to low related (31-75) nitrogen conditions G3431 MYB- 1882 G682 Const. 35S prom. P21324 4788 More tol. to cold (8 C.) related (31-75) G3431 MYB- 1882 G682 Const. 35S prom. P21324 4788 More root hair related (31-75) G3431 MYB- 1882 G682 Const. 35S prom. P21324 4788 Decreased trichome related (31-75) density G735 bZIP (153-237) 570 G735 Const. 35S prom. P121 3835 Greater res. to Botrytis G735 bZIP (153-237) 570 G735 Const. 35S prom. P121 3835 Late flowering G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 Greater tol. to cold (8 C.) 184-276) G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 Less sens. to ABA 184-276) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Less sens. to ABA 184-276) P6506 (35S prom.) G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 More root hair 184-276) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More root hair 184-276) P6506 (35S prom.) G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 Greater tol. to 184-276) hyperosmotic stress; better seedling vigor in 150 mM NaCl G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Greater tol. to 184-276) P6506 (35S prom.) hyperosmotic stress; better seedling vigor in 150 mM NaCl G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 Altered sugar sensing; 184-276) greater tol. to sucrose (determined in 9.4% sucrose) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Altered sugar sensing; 184-276) P6506 (35S prom.) greater tol. to sucrose (determined in 9.4% sucrose) G867 AP2 (59-124, 16 G867 Const. 35S prom. P383 3916 More tol. to drought* 184-276) and better recovery from drought treatment* G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More tol. to drought* 184-276) P6506 (35S prom.) and better recovery from drought treatment* G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More tol. to drought* 184-276) P5311 (ARSK1 and better recovery prom.) from drought treatment* G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Less sens. to ABA 184-276) P5311 (ARSK1 prom.) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Better recovery from 184-276) P5284 (RBCS3 drought treatment* prom.) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Greater tol. to NaCl 184-276) P5284 (RBCS3 (determined with 150 mM prom.) NaCl) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More tol. to drought* 184-276) P9002 (RD29A and better recovery prom.) from drought treatment* G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Greater tol. to NaCl 184-276) P9002 (RD29A (determined with 150 mM prom.) NaCl) G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More tol. to 184-276) P5290 (SUC2 hyperosmotic stress; prom.) greater tol. to 9.4% sucrose or 150 mM NaCl G867 AP2 (59-124, 16 G867 Vascular-specific P21524 4825 Less sens. to ABA 184-276) SUC2 prom. G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 Greater tol. to 184-276) P5290 (SUC2 dehydration prom.) G867 AP2 (59-124, 16 G867 Vascular-specific P21524 4825 Greater tol. to 184-276) SUC2 prom. dehydration G867 AP2 (59-124, 16 G867 2 comp. including P7140 4602 More tol. to drought* 184-276) P5290 (SUC2 and better recovery prom.) from drought treatment* G867 AP2 (59-124, 16 G867 Vascular-specific P21524 4825 More tol. to drought* 184-276) SUC2 prom. and better recovery from drought treatment* G867 AP2 (59-124, 16 G867 RNAi (clade) P21162 4749 Better recovery from 184-276) targeted to drought treatment* conserved domain, 35S G867 AP2 (59-124, 16 G867 RNAi (clade) P21303 4781 Late flowering 184-276) targeted to conserved domain, 35S G867 AP2 (59-124, 16 G867 RNAi (clade) P21303 4781 Greater biomass 184-276) targeted to conserved domain, 35S G867 AP2 (59-124, 16 G867 RNAi (clade) P21162 4749 Greater biomass 184-276) targeted to conserved domain, 35S G867 AP2 (59-124, 16 G867 RNAi (clade) P21304 4782 Greater biomass 184-276) targeted to conserved domain, 35S G867 AP2 (59-124, 16 G867 GAL4 C-term P21193 4750 Altered sugar sensing; 184-276) (Super Active), greater tol. to sucrose 35S (determined in 9.4% sucrose) G867 AP2 (59-124, 16 G867 GAL4 C-term P21193 4750 Multiple alterations 184-276) (Super Active), 35S G867 AP2 (59-124, 16 G867 GAL4 N-term P21201 4752 More tol. to drought* 184-276) (Super Active), and better recovery 35S from drought treatment* G867 AP2 (59-124, 16 G867 GAL4 N-term P21201 4752 Greater tol. to NaCl 184-276) (Super Active), (determined with 150 mM 35S NaCl) G867 AP2 (59-124, 16 G867 GAL4 N-term P21201 4752 Early flowering 184-276) (Super Active), 35S G867 AP2 (59-124, 16 G867 Protein-GFP C P25301 4890 More tol. to drought* 184-276) terminal fusion, and better recovery 35S from drought treatment* G867 AP2 (59-124, 16 G867 Deletion variant, P21275 4773 Greater tol. to 184-276) 35S dehydration G867 AP2 (59-124, 16 G867 Deletion variant, P21276 4774 Early flowering 184-276) 35S G867 AP2 (59-124, 16 G867 Deletion variant, P21276 4774 Decreased trichome 184-276) 35S density G867 AP2 (59-124, 16 G867 Deletion variant, P21275 4773 Greater tol. to cold (8 C.) 184-276) 35S G867 AP2 (59-124, 16 G867 Deletion variant, P21276 4774 Greater tol. to cold (8 C.) 184-276) 35S G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 More root hair 184-277) P6506 (35S prom.) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 More root mass 184-277) G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 More root mass 184-277) P6506 (35S prom.) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Roots have more root 184-277) hairs on methyl jasmonate-containing media G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Greater tol. to cold (8 C.) 184-277) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Less sens. to ABA 184-277) G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 Less sens. to ABA 184-277) P6506 (35S prom.) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 More tol. to 184-277) hyperosmotic stress; greater tol. to 9.4% sucrose or 150 mM NaCl G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 More tol. to 184-277) P6506 (35S prom.) hyperosmotic stress; greater tol. to 9.4% sucrose or 150 mM NaCl G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Greater tol. to NaCl 184-277) (determined with 150 mM NaCl) G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 Greater tol. to NaCl 184-277) P6506 (35S prom.) (determined with 150 mM NaCl) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Greater tol. to sucrose 184-277) (determined in 9.4% sucrose) G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 Greater tol. to sucrose 184-277) P6506 (35S prom.) (determined in 9.4% sucrose) G9 AP2 (62-127, 44 G867 Const. 35S prom. P167 3851 Late flowering 184-277) G9 AP2 (62-127, 44 G867 2 comp. including P7824 4604 Late flowering 184-277) P6506 (35S prom.) G993 AP2 (69-134, 746 G867 Const. 35S prom. P1268 4090 Greater tol. to cold (8 C.) 191-290) G993 AP2 (69-134, 746 G867 Const. 35S prom. P1268 4090 More tol to 191-290) hyperosmotic stress; more tol. to 9.4% sucrose or to 150 mM NaCl G993 AP2 (69-134, 746 G867 Const. 35S prom. P1268 4090 More root hair 191-290) G993 AP2 (69-134, 746 G867 Const. 35S prom. P1268 4090 Greater tol. to NaCl 191-290) (determined with 150 mM NaCl) G1930 AP2 (59-124, 1276 G867 Const. 35S prom. P1310 4106 Decreased trichome 179-273) density G1930 AP2 (59-124, 1276 G867 Const. 35S prom. P1310 4106 Greater tol. to cold (8 C.) 179-273) G1930 AP2 (59-124, 1276 G867 2 comp. including P3373 4507 Greater tol. to cold (8 C.) 179-273) P6506 (35S prom.) G1930 AP2 (59-124, 1276 G867 Const. 35S prom. P1310 4106 Late flowering 179-273) G1930 AP2 (59-124, 1276 G867 2 comp. including P3373 4507 Late flowering 179-273) P6506 (35S prom.) G1930 AP2 (59-124, 1276 G867 Const. 35S prom. P1310 4106 Better germination 179-273) under hyperosmotic stress in 150 mM NaCl or 9.4% sucrose G1930 AP2 (59-124, 1276 G867 Const. 35S prom. P1310 4106 Altered C/N sensing: 179-273) greater tol. to low nitrogen conditions in C/N sensing assay G3455 AP2 (74-143, 1914 G867 Const. 35S prom. P21495 4820 More root hair 201-300) G3455 AP2 (74-143, 1914 G867 Const. 35S prom. P21495 4820 Altered sugar sensing; 201-300) greater tol. to sucrose (determined in 9.4% sucrose) G3455 AP2 (74-143, 1914 G867 Const. 35S prom. P21495 4820 Decreased trichome 201-300) density G3451 AP2 (80-141, 1908 G867 Const. 35S prom. P21500 4821 More tol. to drought* 209-308) and better recovery from drought treatment* G3451 AP2 (80-141, 1908 G867 Const. 35S prom. P21500 4821 Altered sugar sensing; 209-308) greater tol. to sucrose (determined in 9.4% sucrose) G3451 AP2 (80-141, 1908 G867 Const. 35S prom. P21500 4821 More root hair 209-308) G3451 AP2 (80-141, 1908 G867 Const. 35S prom. P21500 4821 Early flowering 209-308) G3452 AP2 (51-116, 1910 G867 Const. 35S prom. P21501 4822 Greater tol. to cold (8 C.) 171-266) G3452 AP2 (51-116, 1910 G867 Const. 35S prom. P21501 4822 More tolerant to 171-266) hyperosmotic stress; greater tol. to 9.4% sucrose or 150 mM NaCl G3452 AP2 (51-116, 1910 G867 Const. 35S prom. P21501 4822 More root hair 171-266) G3452 AP2 (51-116, 1910 G867 Const. 35S prom. P21501 4822 Late flowering 171-266) G3452 AP2 (51-116, 1910 G867 Const. 35S prom. P21501 4822 Early flowering 171-266) G3453 AP2 (57-122, 1912 G867 Const. 35S prom. P23348 4829 Less sens. to ABA 177-272) G3391 AP2 (79-148, 1856 G867 Const. 35S prom. P21257 4763 Greater tol. to NaCl 215-300) (determined with 150 mM NaCl) G3391 AP2 (79-148, 1856 G867 Const. 35S prom. P21257 4763 Early flowering 215-300) G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Greater tol. to cold (8 C.) 177-266) G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Early flowering 177-266) G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Greater tol. to heat (32 C.) 177-266) G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Greater tol. to NaCl 177-266) (determined with 150 mM NaCl) G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Decreased apical 177-266) dominance; bushy inflorescences G3389 AP2 (64-129, 1854 G867 Const. 35S prom. P21260 4764 Greater tol. to drought* 177-266) G3388 AP2 (66-129, 1852 G867 Const. 35S prom. P21266 4767 Leaf orientation 181-274) G3432 AP2 (75-140, 1884 G867 Const. 35S prom. P21318 4786 More tol. to drought* 212-299) and better recovery from drought treatment* G3432 AP2 (75-140, 1884 G867 Const. 35S prom. P21318 4786 Decreased trichome 212-299) density G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 More chlorophyll P6506 (35S prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Photosynthesis rate P6506 (35S prom.) reduced G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Greater tol. to cold (8 C.) P6506 (35S prom.) G913 AP2 (62-128) 682 G913 Const. 35S prom. P929 4001 Altered leaf shape G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Altered leaf shape P6506 (35S prom.) G913 AP2 (62-128) 682 G913 Const. 35S prom. P929 4001 Glossy leaves G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Glossy leaves P6506 (35S prom.) G913 AP2 (62-128) 682 G913 Const. 35S prom. P929 4001 Darker green leaf color G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Darker green leaf color P6506 (35S prom.) G913 AP2 (62-128) 682 G913 Const. 35S prom. P929 4001 More tolerant to freezing G913 AP2 (62-128) 682 G913 Const. 35S prom. P929 4001 Late flowering G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Greater tol. to P9002 (RD29A dehydration prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Greater tol. to cold (8 C.) P9002 (RD29A prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 More tol. to drought* P9002 (RD29A and better recovery prom.) from drought treatment* G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Decreased proline P9002 (RD29A prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Photosynthesis rate P9002 (RD29A reduced prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Late flowering P9002 (RD29A prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Less sens. to ABA P9002 (RD29A prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Darker green leaf color P9002 (RD29A prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Greater tol. to NaCl P9002 (RD29A (determined with 150 mM prom.) NaCl) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Late flowering P5290 (SUC2 prom.) G913 AP2 (62-128) 682 G913 2 comp. including P3598 4516 Darker green leaf color P5290 (SUC2 prom.) G976 AP2 (87-153) 732 G913 Const. 35S prom. P409 3930 Darker green leaf color G976 AP2 (87-153) 732 G913 Const. 35S prom. P409 3930 Waxy leaves G976 AP2 (87-153) 732 G913 Const. 35S prom. P409 3930 Late flowering G2514 AP2 (16-82) 1544 G913 Const. 35S prom P2404.1 5102 Darker green leaf color G1753 AP2 (12-80) 1138 G913 Const. 35S prom. P3326 4499 Altered inflorescence architecture; inflorescences had short internodes, which led to a more compact bushier architecture G1753 AP2 (12-80) 1138 G913 Const. 35S prom. P3326 4499 Altered sugar sensing and/or inc. tol. to hyperosmotic stress; greater tol. to sucrose (determined in 9.4% sucrose) G1753 AP2 (12-80) 1138 G913 Const. 35S prom. P3326 4499 Inc. tol. to hyperosmotic stress (determined in 9.4% sucrose) G1753 AP2 (12-80) 1138 G913 Const. 35S prom. P3326 4499 Darker green leaf color G922 SCR (134-199, 690 G922 2 comp. including P4593 4578 Greater tol. to cold (8 C.) 332-401, P6506 (35S prom.) 405-478) G922 SCR (134-199, 690 G922 Const. 35S prom. P1898 4278 Less sens. to ABA 332-401, 405-478) G922 SCR (134-199, 690 G922 2 comp. including P4593 4578 Less sens. to ABA 332-401, P6506 (35S prom.) 405-478) G922 SCR (134-199, 690 G922 Const. 35S prom. P1898 4278 More tol. to drought* 332-401, and better recovery 405-478) from drought treatment* G922 SCR (134-199, 690 G922 Const. 35S prom. P1898 4278 More tol. to 332-401, hyperosmotic stress; 405-478) better germination on 9.4% sucrose or 150 mM NaCl G922 SCR (134-199, 690 G922 2 comp. including P4593 4578 More tol. to 332-401, P6506 (35S prom.) hyperosmotic stress; 405-478) better germination on 9.4% sucrose or 150 mM NaCl G922 SCR (134-199, 690 G922 2 comp. including P4593 4578 Greater tol. to cold (8 C.) 332-401, P6506 (35S prom.) 405-478) G3810 SCR (106-171, 2138 G922 Const. 35S prom. P25313 4891 Greater tol. to 305-374, dehydration 378-451) G3811 SCR (103-168, 2140 G922 Const. 35S prom. P25424 4899 Altered leaf shape 296-365, 369-442) G3811 SCR (103-168, 2140 G922 Const. 35S prom. P25424 4899 Darker green leaf color 296-365, 369-442) G3824 SCR (42-107, 2146 G922 — — n/d 235-304, 308-381) G3813 SCR (129-194, 2142 G922 — — n/d 290-359, 363-436) G3814 SCR (123-190, 2144 G922 — — n/d 332-400, 404-480) G3827 SCR (226-295, 2148 G922 — — n/d 299-365) G975 AP2 (4-71) 730 G975 Const. 35S prom. P408 3929 Altered leaf biochemistry; dark, shiny, waxy leaves, more fatty acids and wax in leaves G975 AP2 (4-71) 730 G975 Const. 35S prom. P408 3929 More tol. to drought* and better recovery from drought treatment* G975 AP2 (4-71) 730 G975 2 comp. including P3367 4503 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G975 AP2 (4-71) 730 G975 Const. 35S prom. P408 3929 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G975 AP2 (4-71) 730 G975 2 comp. including P3367 4503 Altered sugar sensing; P5288 (CUT1 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G975 AP2 (4-71) 730 G975 2 comp. including P3367 4503 Greater tol. to cold (8 C.) P5288 (CUT1 prom.) G1387 AP2 (4-71) 960 G975 — — n/d G2583 AP2 (4-71) 1590 G975 Const. 35S prom. P2002 4305 Glossy, shiny leaves G4294 AP2 (5-72) 2346 G975 — — n/d G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 Altered branching, (63-71, 71-216) short internodes G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 Greater to substantially (63-71, 71-216) greater plant size G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 Greater seed yield (63-71, 71-216) G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 More root hair (63-71, 71-216) G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 Greater root mass (63-71, 71-216) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to (63-71, 71-216) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G1073 AT-hook 18 G1073 Const. 35S prom. P25703 4919 Greater tol. to (63-71, 71-216) dehydration G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 More tol. to drought* (63-71, 71-216) and better recovery from drought treatment* G1073 AT-hook 18 G1073 Const. 35S prom. P25703 4919 More tol. to drought* (63-71, 71-216) and better recovery from drought treatment* G1073 AT-hook 18 G1073 2 comp. including P3369 4504 More tol. to drought* (63-71, 71-216) P6506 (35S prom.) and better recovery from drought treatment* G1073 AT-hook 18 G1073 Const. 35S prom. P448 3936 Large flower (63-71, 71-216) G1073 AT-hook 18 G1073 Const. 35S prom. P25703 4919 Large flower (63-71, 71-216) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Large flower (63-71, 71-216) P6506 (35S prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to cold (8 C.) (63-71, 71-216) P5326 (AP1 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to NaCl (63-71, 71-216) P5311 (ARSK1 (determined with 150 mM prom.) NaCl) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 More tol. to drought* (63-71, 71-216) P5311 (ARSK1 and better recovery prom.) from drought treatment* G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to NaCl (63-71, 71-216) P5311 (ARSK1 (determined with 150 mM prom.) NaCl) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to cold (8 C.) (63-71, 71-216) P5319 (AS1 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater seedling vigor (63-71, 71-216) P5319 (AS1 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 More tol. to (63-71, 71-216) P5288 (CUT1 hyperosmotic stress; prom.) greater tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G1073 AT-hook 18 G1073 2 comp. including P3369 4504 More tol. to (63-71, 71-216) P5288 (CUT1 hyperosmotic stress; prom.) greater tol. to 9.4% sucrose, 300 mM mannitol or 150 mM NaCl G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to heat (32 C.) (63-71, 71-216) P5284 (RBCS3 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Late flowering (63-71, 71-216) P5284 (RBCS3 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater biomass (63-71, 71-216) P5284 (RBCS3 prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to NaCl (63-71, 71-216) P5284 (RBCS3 (determined with 150 mM prom.) NaCl) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to (63-71, 71-216) P5318 (STM dehydration prom.) G1073 AT-hook 18 G1073 2 comp. including P3369 4504 More tol. to drought* (63-71, 71-216) P5318 (STM and better recovery prom.) from drought treatment* G1073 AT-hook 18 G1073 Vascular-specific P21521 4823 More tol. to drought* (63-71, 71-216) SUC2 prom. and better recovery from drought treatment* G1073 AT-hook 18 G1073 Vascular-specific P21521 4823 Greater biomass (63-69, 71-216) SUC2 prom. G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater biomass (63-69, 71-216) P5290 (SUC2 prom.) G1073 AT-hook 18 G1073 Vascular-specific P21521 4823 Greater tol. to cold (8 C.) (63-71, 71-216) SUC2 prom. G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Greater tol. to cold (8 C.) (63-71, 71-216) P5290 (SUC2 prom.) G1073 AT-hook 18 G1073 Vascular-specific P21521 4823 Late flowering (63-71, 71-216) SUC2 prom. G1073 AT-hook 18 G1073 2 comp. including P3369 4504 Late flowering (63-71, 71-216) P5290 (SUC2 prom.) G1073 AT-hook 18 G1073 GAL4 N-term P21199 4751 Late flowering (63-71, 71-216) (Super Active), 35S G1073 AT-hook 18 G1073 GAL4 N-term P21199 4751 Less sens. to ABA (63-71, 71-216) (Super Active), 35S G1073 AT-hook 18 G1073 GAL4 N-term P21199 4751 Altered leaf shape (63-71, 71-216) (Super Active), 35S G1073 AT-hook 18 G1073 GAL4 N-term P21199 4751 Darker green leaf color (63-71, 71-216) (Super Active), 35S G1073 AT-hook 18 G1073 GAL4 C-term P21145 4745 Greater tol. to (63-71, 71-216) (Super Active), dehydration 35S G1073 AT-hook 18 G1073 GAL4 C-term P21145 4745 More tol. to drought* (63-71, 71-216) (Super Active), and better recovery 35S from drought treatment* G1073 AT-hook 18 G1073 Protein-GFP C P25263 4884 More tol. to drought* (63-71, 71-216) terminal fusion, and better recovery 35S from drought treatment* G1073 AT-hook 18 G1073 RNAi (clade) P21301 4780 Greater tol. to (63-71, 71-216) targeted to dehydration conserved domain, 35S G1073 AT-hook 18 G1073 RNAi (clade) P21160 4748 Greater tol. to (63-71, 71-216) targeted to dehydration conserved domain, 35S G1073 AT-hook 18 G1073 RNAi Gene- P21117 4743 Greater tol, to (63-71, 71-216) Specific (GS), 35S dehydration G1073 AT-hook 18 G1073 RNAi Gene- P21117 4743 Greater tol, to NaCl (63-71, 71-216) Specific (GS), 35S (determined with 150 mM NaCl) G1073 AT-hook 18 G1073 Deletion variant, P21271 4770 Greater biomass (63-71, 71-216) 35S G1073 AT-hook 18 G1073 Deletion variant, P21272 4771 Altered leaf shape (63-71, 71-216) 35S G1073 AT-hook 18 G1073 Knockout not Greater tol. to drought* (63-71, 71-216) applicable G1073 AT-hook 18 G1073 Knockout not Greater root mass (63-71, 71-216) applicable G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Larger leaf size (67-75, 75-218) G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Altered leaf shape (67-75, 75-218) G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Less sens. to ABA (67-75, 75-218) G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Altered sugar sensing; (67-75, 75-218) greater tol. to sucrose (determined in 9.4% sucrose) G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Altered C/N sensing: (67-75, 75-218) greater tol. to low nitrogen conditions in C/N sensing assay G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Altered leaf (67-75, 75-218) glucosinolate composition; inc. M39497 G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 Altered light response; (67-75, 75-218) greater shade tol.; lack of shade avoidance phenotype G1069 AT-hook 802 G1073 Const. 35S prom. P1178 4058 More tol. to drought* (67-75, 75-218) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to cold (8 C.) (86-94, 94-247) P6506 (35S prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 More tol. to drought* (86-94, 94-247) P6506 (35S prom.) and better recovery from drought treatment* G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Altered leaf shape; (86-94, 94-247) P6506 (35S prom.) twisted and up-curled rosette leaves G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Smaller plants (86-94, 94-247) P6506 (35S prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Reduced fertility (86-94, 94-247) P6506 (35S prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Less sens. to ABA (86-94, 94-247) P6506 (35S prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to (86-94, 94-247) P5311 (ARSK1 dehydration prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to NaCl (86-94, 94-247) P5311 (ARSK1 (determined with 150 mM prom.) NaCl) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to NaCl (86-94, 94-247) P5311 (ARSK1 (determined with 150 mM prom.) NaCl) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Larger leaf size (86-94, 94-247) P5284 (RBCS3 prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 More tol. to drought* (86-94, 94-247) P5284 (RBCS3 and better recovery prom.) from drought treatment* G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Late flowering (86-94, 94-247) P5284 (RBCS3 prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Altered leaf shape (86-94, 94-247) P5284 (RBCS3 prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to NaCl (86-94, 94-247) P5284 (RBCS3 (determined with 150 mM prom.) NaCl) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to (86-94, 94-247) P9002 (RD29A dehydration prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 More tol. to drought* (86-94, 94-247) P9002 (RD29A and better recovery prom.) from drought treatment* G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Larger leaf size (86-94, 94-247) P9002 (RD29A prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 More root hair (86-94, 94-247) P9002 (RD29A prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Late flowering (86-94, 94-247) P9002 (RD29A prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Altered leaf shape (86-94, 94-247) P9002 (RD29A prom.) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to 300 mM (86-94, 94-247) P9002 (RD29A mannitol or to NaCl prom.) (determined with 150 mM NaCl) G1067 AT-hook 798 G1073 2 comp. including P7832 4606 Greater tol. to NaCl (86-94, 94-247) P9002 (RD29A (determined with 150 mM prom.) NaCl) G1667 AT-hook 1116 G1073 Const. 35S prom. P1079 4046 Inc. seed protein, (53-61, 61-204) decreased seed oil, inc. leaf ?-carotene levels G1075 AT-hook 804 G1073 Const. 35S prom. P450 3937 Reduced or absent (78-86, 86-229) flower petals, sepals or stamens G1075 AT-hook 804 G1073 Const. 35S prom. P450 3937 Reduced fertility (78-86, 86-229) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 Less sens. to ABA (80-88, 88-239) G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 Less sens. to ABA (80-88, 88-239) P6506 (35S prom.) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 Greater tol. to cold (8 C.) (80-88, 88-239) G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 Greater tol. to cold (8 C.) (80-88, 88-239) P6506 (35S prom.) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 Large flower (80-88, 88-239) G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 Large flower (80-88, 88-239) P6506 (35S prom.) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 Late flowering (80-88, 88-239) G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 Late flowering (80-88, 88-239) P6506 (35S prom.) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 Greater biomass (80-88, 88-239) G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 Greater biomass (80-88, 88-239) P6506 (35S prom.) G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 More tol. to drought* (80-88, 88-239) and better recovery from drought treatment* G2153 AT-hook 1420 G1073 Const. 35S prom. P1740 4245 More tol to (80-88,88-239) hyperosmotic stress; better germination in 9.4% sucrose or 150 mM NaCl G2153 AT-hook 1420 G1073 2 comp. including P4524 4568 More tol to (80-88, 88-239) P6506 (35S prom.) hyperosmotics stress; better germination in 9.4% sucrose or 150 mM NaCl G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Less sens. to ABA (72-80, 80-232) P6506 (35S prom.) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Large flower (72-80, 80-232) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Large flower (72-80, 80-232) P6506 (35S prom.) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Larger leaf size (72-80, 80-232) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Greater biomass (72-80, 80-232) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Late flowering (72-80, 80-232) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Late flowering (72-80, 80-232) P6506 (35S prom.) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Greater tol. to cold (8 C.) (72-80, 80-232) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Greater tol. to cold (8 C.) (72-80, 80-232) P6506 (35S prom.) G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 Greater tol. to (72-80, 80-232) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Greater tol. to (72-80, 80-232) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G2156 AT-hook 1424 G1073 Const. 35S prom. P1721 4238 More tol. to drought* (72-80, 80-232) and better recovery from drought treatment* G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Late flowering (72-80, 80-232) P5311 (ARSK1 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Greater tol. to (72-80, 80-232) P5311 (ARSK1 dehydration prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Larger leaf size (72-80, 80-232) P5284 (RBCS3 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Greater biomass (72-80, 80-232) P5284 (RBCS3 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Late flowering (72-80, 80-232) P5284 (RBCS3 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Less sens. to ABA (72-80, 80-232) P5284 (RBCS3 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Altered leaf shape (72-80, 80-232) P5284 (RBCS3 prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Late flowering (72-80, 80-232) P9002 (RD29A prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Less sens. to ABA (72-80, 80-232) P9002 (RD29A prom.) G2156 AT-hook 1424 G1073 2 comp. including P4418 4565 Greater biomass (72-80, 80-232) P9002 (RD29A prom.) G2157 AT-hook 1426 G1073 Const. 35S prom. P1722 4239 Altered leaf shape (88-96, 96-240) G2157 AT-hook 1426 G1073 Const. 35S prom. P1722 4239 Greater tol. to (88-96, 96-240) dehydration G2157 AT-hook 1426 G1073 Const. 35S prom. P1722 4239 Larger leaf size (88-96, 96-240) G2157 AT-hook 1426 G1073 2 comp. including P4419 4566 Significantly greater (88-96, 96-240) P5326 (AP1 tomato plant volume prom.) G2157 AT-hook 1426 G1073 2 comp. including P4419 4566 Significantly greater (88-96, 96-240) P5287 (LTP1 tomato plant volume prom.) G2157 AT-hook 1426 G1073 2 comp. including P4419 4566 Significantly greater (88-96, 96-240) P5318 (STM plant volume in tomato prom.) plants G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 More tol. to drought* (44-52, 52-195) and better recovery from drought treatment* G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Greater tol. to cold (8 C.) (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Greater tol. to NaCl (44-52, 52-195) (determined with 150 mM NaCl) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Larger leaf size (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Larger leaf size (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Greater biomass (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Greater biomass (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Darker green leaf color (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Darker green leaf color (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Delayed senescence (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Delayed senescence (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Decreased apical (44-52, 52-195) dominance; slightly short inflorescence internodes leading to a somewhat bushy architecture G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Decreased apical (44-52, 52-195) dominance; slightly short inflorescence internodes leading to a somewhat bushy architecture G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Late flowering (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Late flowering (44-52, 52-195) G3456 AT-hook 1916 G1073 Const. 35S prom. P21328 4789 Altered leaf shape; (44-52, 52-195) curled leaves G3456 AT-hook 1916 G1073 Const. 35S prom. P21467 4816 Altered leaf shape; (44-52, 52-195) curled leaves G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Greater tol. to cold (8 C.) (77-85, 85-228) G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Multiple alterations (77-85, 85-228) G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Late flowering (77-85, 85-228) G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Greater tol. to heat (32 C.) (77-85, 85-228) G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Larger leaf size (77-85, 85-228) G3459 AT-hook 1918 G1073 Const. 35S prom. P21331 4790 Greater tol. to NaCl (77-85, 85-228) (determined with 150 mM NaCl) G3460 AT-hook 1920 G1073 Const. 35S prom. P21332 4791 Greater biomass (74-82, 82-225) G3460 AT-hook 1920 G1073 Const. 35S prom. P21332 4791 More tol. to drought* (74-82, 82-225) and better recovery from drought treatment* G3460 AT-hook 1920 G1073 Const. 35S prom. P21332 4791 Greater tol. to heat (32 C.) (74-82, 82-225) G3460 AT-hook 1920 G1073 Const. 35S prom. P21332 4791 Darker green leaf color (74-82, 82-225) G3460 AT-hook 1920 G1073 Const. 35S prom. P21332 4791 Late flowering (74-82, 82-225) G3407 AT-hook 1876 G1073 Const. 35S prom. P21243 4753 Greater seedling vigor (63-71, 71-220) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Greater biomass (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Large flower (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Late flowering (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Larger leaf size (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Altered leaf shape (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 Greater tol. to cold (8 C.) (83-91, 91-237) G3400 AT-hook 1872 G1073 Const. 35S prom. P21244 4754 More tol. to drought* (83-91, 91-237) and better recovery from drought treatment* G3401 AT-hook 1874 G1073 Const. 35S prom. P21264 4765 More tol. to drought* (35-43, 43-186) and better recovery from drought treatment* G3401 AT-hook 1874 G1073 Const. 35S prom. P21264 4765 Late flowering (35-43, 43-186) G3401 AT-hook 1874 G1073 Const. 35S prom. P21264 4765 Larger leaf size (35-43, 43-186) G3401 AT-hook 1874 G1073 Const. 35S prom. P21264 4765 Altered sugar sensing; (35-43, 43-186) greater tol. to sucrose (determined in 9.4% sucrose) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 More tol. to drought* (99-107, and better recovery 107-253) from drought treatment* G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 Large flower (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 Greater tol. to (99-107, dehydration 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21269 4769 Greater biomass (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 Greater biomass (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21269 4769 Late flowering (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 Late flowering (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21269 4769 Larger leaf size (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 Larger leaf size (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21269 4769 More root hair (99-105, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 More root hair (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21269 4769 More root mass (99-107, 107-253) G3399 AT-hook 1870 G1073 Const. 35S prom. P21465 4814 More root mass (99-107, 107-253) G3556 AT-hook 2034 G1073 Const. 35S prom. P21493 4819 Greater tol. to (45-53, 53-196) dehydration G3556 AT-hook 2034 G1073 Const. 35S prom. P21493 4819 Greater tol. to NaCl (45-53, 53-196) (determined with 150 mM NaCl) G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Greater res. to Botrytis G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Greater res. to Erysiphe G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Greater res. to Sclerotinia G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Less sens. to ABA G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Late flowering G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Darker green leaf color G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Reduced sens. to ABA G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1266 AP2 (79-147) 884 G1266 Const. 35S prom. P483 3944 Altered leaf insoluble sugars, including rhamnose, arabinose, xylose, and mannose, and galactose G1274 WRKY 20 G1274 2 comp. including P8239 4609 Less sens. to ABA (110-166) P6506 (35S prom.) G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 Greater res. to (110-166) Erysiphe G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 Trilocular silique (110-166) G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 Greater seed number (110-166) G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 Altered sugar sensing; (110-166) greater tol. to sucrose (determined in 9.4% sucrose) G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 Greater tol. to cold (8 C.) (110-166) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to cold (8 C.) (110-166) P6506 (35S prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Large leaves, greater (110-166) P6506 (35S prom.) biomass G1274 WRKY 20 G1274 Const. 35S prom. P15038 4665 More tol. to drought* (110-166) and better recovery from drought treatment* G1274 WRKY 20 G1274 2 comp. including P8239 4609 More tol. to drought* (110-166) P6506 (35S prom.) and better recovery from drought treatment* G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to (110-166) P5311 (ARSK1 dehydration prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 More root hair (110-166) P5319 (AS1 prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 More root mass (110-166) P5319 (AS1 prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Altered C/N sensing: (110-166) P5288 (CUT1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 2 comp. including P8239 4609 Less sens. to ABA (110-166) P5284 (RBCS3 prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Altered C/N sensing: (110-166) P5284 (RBCS3 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to 300 mM (110-166) P5284 (RBCS3 mannitol prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Better recovery from (110-166) P9002 (RD29A drought treatment* prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to cold (8 C.) (110-166) P5318 (STM prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to (110-166) P5318 (STM dehydration prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Better recovery from (110-166) P5318 (STM drought treatment* prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Greater tol. to (110-166) P5290 (SUC2 dehydration prom.) G1274 WRKY 20 G1274 2 comp. including P8239 4609 Altered C/N sensing: (110-166) P5290 (SUC2 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 GAL4 N-term P25659 4915 Greater tol. to (110-166) (Super Active), dehydration 35S G1274 WRKY 20 G1274 GAL4 C-term P25658 4914 Decreased apical (110-166) (Super Active), dominance; short 35S bushy inflorescences G1274 WRKY 20 G1274 GAL4 C-term P25658 4914 Larger leaf size (110-166) (Super Active), 35S G1274 WRKY 20 G1274 GAL4 C-term P25658 4914 Greater tol. to (110-166) (Super Active), dehydration 35S G1274 WRKY 20 G1274 Point mutation, P25744 4929 Less sens. to ABA (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25744 4929 Greater tol. to (110-166) 35S dehydration G1274 WRKY 20 G1274 Point mutation, P25746 4931 Greater tol. to low (110-166) 35S nitrogen conditions G1274 WRKY 20 G1274 Point mutation, P25742 4927 Altered sugar sensing; (110-166) 35S greater tol. to sucrose (determined in 9.4% sucrose) G1274 WRKY 20 G1274 Point mutation, P25746 4931 Altered sugar sensing; (110-166) 35S greater tol. to sucrose (determined in 9.4% sucrose) G1274 WRKY 20 G1274 Point mutation, P25742 4927 Altered C/N sensing: (110-166) 35S greater tol. to low nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 Point mutation, P25743 4928 Altered C/N sensing: (110-166) 35S greater tol. to low nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 Point mutation, P25745 4930 Altered C/N sensing: (110-166) 35S greater tol. to low nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 Point mutation, P25742 4927 Greater tol. to cold (8 C.) (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25743 4928 Greater tol. to cold (8 C.) (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25745 4930 Greater tol. to cold (8 C.) (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25746 4931 Greater tol. to cold (8 C.) (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25742 4927 More tol. to drought* (110-166) 35S and show better recovery from drought treatment* G1274 WRKY 20 G1274 Point mutation, P25743 4928 More tol. to drought* (110-166) 35S and show better recovery from drought treatment* G1274 WRKY 20 G1274 Point mutation, P25745 4930 More tol. to drought* (110-166) 35S and show better recovery from drought treatment* G1274 WRKY 20 G1274 Point mutation, P25746 4931 More tol. to drought* (110-166) 35S and show better recovery from drought treatment* G1274 WRKY 20 G1274 Point mutation, P25742 4927 Larger leaf size (110-166) 35S G1274 WRXY 20 G1274 Point mutation, P25743 4928 Larger leaf size (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25745 4930 Larger leaf size (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25746 4931 Larger leaf size (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25744 4929 Larger leaf size (110-166) 35S G1274 WRKY 20 G1274 Point mutation, P25742 4927 Inflorescence: (110-166) 35S decreased apical dominance G1274 WRKY 20 G1274 Point mutation, P25743 4928 Inflorescence: (110-166) 35S decreased apical dominance G1274 WRKY 20 G1274 Point mutation, P25745 4930 Inflorescence: (110-166) 35S decreased apical dominance G1274 WRKY 20 G1274 Point mutation, P25746 4931 Inflorescence: (110-166) 35S decreased apical dominance G1274 WRKY 20 G1274 Point mutation, P25744 4929 Inflorescence: (110-166) 35S decreased apical dominance G1274 WRKY 20 G1274 Domain P25435 4901 Greater tol. to cold (8 C.) (110-166) swap/chimeric variant, 35S G1274 WRKY 20 G1274 Domain P25435 4901 Altered C/N sensing: (110-166) swap/chimeric greater tol. to low variant, 35S nitrogen conditions in C/N sensing assay G1274 WRKY 20 G1274 Domain P25435 4901 Greater tol. to (110-166) swap/chimeric dehydration variant, 35S G1274 WRKY 20 G1274 Domain P25435 4901 Larger leaf size (110-166) swap/chimeric variant, 35S G1274 WRKY 20 G1274 Domain P25435 4901 Altered sugar sensing; (110-166) swap/chimeric greater tol. to sucrose variant, 35S (determined in 9.4% sucrose) G1274 WRKY 20 G1274 Knockout not Altered C/N sensing: (110-166) applicable greater tol. to low nitrogen conditions in C/N sensing assay G1275 WRKY 894 G1274 Const. 35S prom. P486 3946 Greater tol. to cold (8 C.) (113-169) G1275 WRKY 894 G1274 Const. 35S prom. P486 3946 Greater tol. to heat (32 C.) (113-169) G1275 WRKY 894 G1274 Const. 35S prom. P486 3946 Reduced apical (113-169) dominance G1275 WRKY 894 G1274 Const. 35S prom. P486 3946 Smaller plants (113-169) G1275 WRKY 894 G1274 2 comp. including P3412 4511 More root mass (113-169) P5319 (AS1 prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Larger leaf size (113-169) P5319 (AS1 prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Greater tol. to cold (8 C.) (113-169) P5288 (CUT1 prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Better recovery from (113-169) P5288 (CUT1 drought treatment* prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Altered C/N sensing: (113-169) P5288 (CUT1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1275 WRKY 894 G1274 2 comp. including P3412 4511 Altered sugar sensing; (113-169) P5288 (CUT1 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G1275 WRKY 894 G1274 2 comp. including P3412 4511 More tol. to drought* (113-169) P9002 (RD29A and better recovery prom.) from drought treatment* G1275 WRKY 894 G1274 2 comp. including P3412 4511 Less sens. to ABA (113-169) P9002 (RD29A prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Greater tol. to low (113-169) P5318 (STM nitrogen conditions prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Altered C/N sensing: (113-169) P5290 (SUC2 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1275 WRKY 894 G1274 2 comp. including P3412 4511 Late flowering (113-169) P5290 (SUC2 prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Darker green leaf color (113-169) P5290 (SUC2 prom.) G1275 WRKY 894 G1274 2 comp. including P3412 4511 Decreased root mass (113-169) P5290 (SUC2 prom.) G194 WRKY 162 G1274 Const. 35S prom. P197 3863 Greater tol. to (174-230) dehydration G194 WRKY 162 G1274 Const. 35S prom. P197 3863 Small plant (174-230) G1758 WRKY 1144 G1274 Const. 35S prom. P1224 4071 Greater tol. to cold (8 C.) (109-165) G2517 WRKY 1548 G1274 Const. 35S prom. P1833 4268 Greater tol. to (117-177) dehydration G2517 WRKY 1548 G1274 Const. 35S prom. P1833 4268 Early flowering (117-177) G2517 WRKY 1548 G1274 Const. 35S prom. P1833 4268 More tol. to glyphosate (117-177) G179 WRKY (65-121) 138 G1274 Domain P25439 4904 Less sens. to ABA swap/chimeric variant, 35S G179 WRKY (65-121) 138 G1274 Domain P25439 4904 Altered sugar sensing; swap/chimeric greater tol. to sucrose variant, 35S (determined in 9.4% sucrose) G3723 WRKY 2088 G1274 Const. 35S prom. P25208 4868 Inflorescence: (112-168) decreased apical dominance G3723 WRKY 2088 G1274 Const. 35S prom. P25208 4868 Larger leaf size (112-168) G3723 WRKY 2088 G1274 Const. 35S prom. P25208 4868 Altered leaf shape (112-168) G3723 WRKY 2088 G1274 Const. 35S prom. P25208 4868 Greater seedling vigor (112-168) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Greater tol. to cold (8 C.) (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Less sens. to ABA (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Larger leaf size (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 More root mass (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Greater biomass (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Greater tol. to NaCl (107-163) (determined with 150 mM NaCl) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 More tol. to drought* (107-163) and better recovery from drought treatment* G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Late flowering (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Altered leaf shape (107-163) G3724 WRKY 2090 G1274 Const. 35S prom. P25384 4895 Greater tol. to (107-163) hyperosmotic stress; more tol. to 9.4% sucrose or to 150 mM NaCl G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Inflorescence: (111-167) decreased apical dominance G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Decreased tol. to cold (111-167) (8 C.) G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Late flowering (111-167) G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Early flowering (111-167) G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Altered leaf shape (111-167) G3803 WRKY 2134 G1274 Const. 35S prom. P25218 4874 Altered silique (111-167) development G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 Greater tol. to cold (8 C.) G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 More tol. to drought* and better recovery from drought treatment* G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 Less sens. to ABA G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 Greater tol. to NaCl (determined with 150 mM NaCl) G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 Inflorescence: decreased apical dominance G3721 WRKY (96-152) 2084 G1274 Const. 35S prom. P25368 4893 Greater tol. to 300 mM mannitol or to NaCl (determined with 150 mM NaCl) G3725 WRKY 2092 G1274 Const. 35S prom. P25210 4869 More root mass (158-214) G3726 WRKY 2094 G1274 Const. 35S prom. P25211 4870 Inflorescence: (135-191) decreased apical dominance G3726 WRKY 2094 G1274 Const. 35S prom. P25211 4870 Greater tol. to cold (8 C.) (135-191) G3726 WRKY 2094 G1274 Const. 35S prom. P25211 4870 More tol. to drought* (135-191) and better recovery from drought treatment* G3726 WRKY 2094 G1274 Const. 35S prom. P25211 4870 Early flowering (135-191) G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Altered C/N sensing: (137-193) greater tol. to low nitrogen conditions in C/N sensing assay G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Greater tol. to cold (8 C.) (137-193) G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Larger leaf size (137-193) G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Trilocular silique (137-193) G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Greater seed number (137-193) G3729 WRKY 2100 G1274 Const. 35S prom. P25214 4872 Greater biomass (137-193) G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Inflorescence: (107-163) decreased apical dominance G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Late flowering (107-163) G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Altered leaf shape (107-163) G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Leaf orientation (107-163) G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Trilocular silique (107-163) G3730 WRKY 2102 G1274 Const. 35S prom. P25215 4873 Greater seed number (107-163) G3719 WRKY (98-154) 2080 G1274 Const. 35S prom. P25204 4865 Inflorescence: decreased apical dominance G3720 WRKY 2082 G1274 Const. 35S prom. P25205 4866 Inflorescence: (135-191) decreased apical dominance G3720 WRKY 2082 G1274 Const. 35S prom. P25205 4866 Greater tol. to low (135-191) nitrogen conditions G3722 WRKY 2086 G1274 Const. 35S prom. P25207 4867 Inflorescence: (129-185) decreased apical dominance G3722 WRKY 2086 G1274 Const. 35S prom. P25207 4867 Altered C/N sensing: (129-185) greater tol. to low nitrogen conditions in C/N sensing assay G3727 WRKY 2096 G1274 Const. 35S prom. P25385 4896 Inflorescence: (102-158) decreased apical dominance G3727 WRKY 2096 G1274 Const. 35S prom. P25385 4896 Early flowering (102-158) G3727 WRKY 2096 G1274 Const. 35S prom. P25385 4896 Greater tol. to low (102-158) nitrogen conditions G3727 WRKY 2096 G1274 Const. 35S prom. P25385 4896 Trilocular silique (102-158) G3727 WRKY 2096 G1274 Const. 35S prom. P25385 4896 Greater seed number (102-158) G3728 WRKY 2098 G1274 Const. 35S prom. P25213 4871 Inflorescence: (108-164) decreased apical dominance G3728 WRKY 2098 G1274 Const. 35S prom. P25213 4871 Altered silique (108-164) development G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 More tol. to drought* (108-164) and better recovery from drought treatment* G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 Greater tol. to cold (8 C.) (108-164) G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 Greater tol. to cold (8 C.) (108-164) G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 Early flowering (108-164) G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 Altered leaf shape (108-164) G3804 WRKY 2136 G1274 Const. 35S prom. P25219 4875 Trilocular silique (108-164) G1543 HB (135-195) 1062 G1543 Const. 35S prom. P1051 4038 Altered architecture, compact plant G1543 HB (135-195) 1062 G1543 Const. 35S prom. P1051 4038 Darker green color G1543 HB (135-195) 1062 G1543 Const. 35S prom. P1051 4038 Decreased seed oil content G1543 HB (135-195) 1062 G1543 Const. 35S prom. P1051 4038 Altered leaf prenyl lipids; more chlorophyll a and b G1543 HB (135-195) 1062 G1543 2 comp. including P3424 4512 Significantly greater P5287 (LTP1 tomato plant volume prom.) G1543 HB (135-195) 1062 G1543 2 comp. including P3424 4512 Significantly greater P5297 (PG prom.) tomato plant volume G3524 HB (60-120) 1988 G1543 — — n/d G3510 HB (74-134) 1974 G1543 — — n/d G3490 HB (60-120) 1958 G1543 — — n/d G1752 AP2 (83-151) 1136 G1752 Const. 35S prom. P1636 4213 Greater res. to Erysiphe G1752 AP2 (83-151) 1136 G1752 Const. 35S prom. P1636 4213 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1752 AP2 (83-151) 1136 G1752 Const. 35S prom. P1636 4213 Greater tol. to 300 mM mannitol G1752 AP2 (83-151) 1136 G1752 2 comp. including P4390 4555 Significantly greater P6506 (35S prom.) tomato plant volume G1752 AP2 (83-151) 1136 G1752 2 comp. including P4390 4555 Significantly greater P5324 (Cru prom.) tomato plant volume G1752 AP2 (83-151) 1136 G1752 2 comp. including P4390 4555 Significantly greater P5297 (PG prom.) tomato plant volume G2512 AP2 (79-147) 1540 G1752 Const. 35S prom. P1829 4265 Greater res. to Erysiphe G2512 AP2 (79-147) 1540 G1752 Const. 35S prom. P1829 4265 Inc. leaf glucosinolate M39481 G2512 AP2 (79-147) 1540 G1752 Const. 35S prom. P1829 4265 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1760 MADS (2-57) 22 G1760 2 comp. including P3371 4505 Greater tol. to cold (8 C.) P6506 (35S prom.) G1760 MADS (2-57) 22 G1760 Const. 35S prom. P1461 4152 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1760 MADS (2-57) 22 G1760 2 comp. including P3371 4505 Altered C/N sensing: P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G1760 MADS (2-57) 22 G1760 2 comp. including P3371 4505 Altered sugar sensing; P6506 (35S prom.) greater tol. to sucrose (determined in 9.4% sucrose) G152 MADS (2-57) 110 G1760 Const. 35S prom. P896 3996 Only 3 lines produced, no positive physiological results at this time G153 MADS (2-57) 112 G1760 Const. 35S prom. P15260 4691 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G153 MADS (2-57) 112 G1760 Const. 35S prom. P15260 4691 Greater tol. to low nitrogen conditions G153 MADS (2-57) 112 G1760 Const. 35S prom. P15260 4691 Early flowering G860 MADS (2-57) 640 G1760 Const. 35S prom. P1269 4091 Only 3 lines produced, no positive physiological results at this time G3484 MADS (2-57) 1948 G1760 Const. 35S prom. P26744 5049 Reduced or delayed floral organ abscission G3484 MADS (2-57) 1948 G1760 Const. 35S prom. P26744 5049 Early flowering G3485 MADS (2-57) 1950 G1760 — — n/d G3980 MADS (2-57) 2246 G1760 Const. 35S prom. P26799 5052 Early flowering G3981 MADS (2-57) 2248 G1760 — — n/d G3479 MADS (2-57) 1938 G1760 Const. 35S prom. P26738 5048 Early flowering G3480 MADS (2-57) 1940 G1760 — — n/d G3481 MADS (2-57) 1942 G1760 — — n/d G3482 MADS (2-57) 1944 G1760 — — n/d G3483 MADS (2-57) 1946 G1760 — — n/d G3487 MADS (2-57) 1952 G1760 — — n/d G3488 MADS (2-57) 1954 G1760 — — n/d G3489 MADS (2-57) 1956 G1760 — — n/d G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P6071 4598 Greater tol. to cold (8 C.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered C/N sensing: P6506 (35S prom.) greater tol. to low nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 More tol. to nitrogen- limited medium G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More root hair P6506 (35S prom.) G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 More root hair G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More root mass P6506 (35S prom.) G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 More root mass G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Two lines of plants had higher chlorophyll content and higher total nitrogen concentration G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered leaf shape P6506 (35S prom.) G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Altered leaf shape G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P26498 5016 Altered leaf shape G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Darker green leaf P6506 (35S prom.) color, shiny leaves G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Darker green leaf color, shiny leaves G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P26498 5016 Darker green leaf color, shiny leaves G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Greater resistance to Erysiphe G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Greater resistance to Botrytis G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Greater resistance to Fusarium G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More tol. to P6506 (35S prom.) dehydration G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 More tol. to dehydration G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P26498 5016 More tol. to dehydration G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 Inc. seed oil content G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More tol. to drought* P6506 (35S prom.) and better recovery from drought treatment* G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P1695 4227 More tol. to drought* and better recovery from drought treatment* G1792 AP2 (16-80) 24 G1792 Const. 35S prom. P26498 5016 More tol. to drought* and better recovery from drought treatment* G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered sugar sensing; P5326 (AP1 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to P5326 (AP1 dehydration prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered C/N sensing: P5319 (AS1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered C/N sensing: P5288 (CUT1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to cold (8 C.) P5288 (CUT1 prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Better recovery from P5288 (CUT1 drought treatment* prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Late flowering P5288 (CUT1 prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to low P5287 (LTP1 nitrogen conditions prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More res. to Botrytis P5284 (RBCS3 prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Darker green leaf color P5284 (RBCS3 prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to NaCl P5284 (RBCS3 (determined with 150 mM prom.) NaCl) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 More tol. to drought* P9002 (RD29A and better recovery prom.) from drought treatment* G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Less sens. to ABA P9002 (RD29A prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to low P9002 (RD29A nitrogen conditions prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to NaCl P9002 (RD29A (determined with 150 mM prom.) NaCl) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered C/N sensing: P5310 (RS1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to cold (8 C.) P5318 (STM prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to 300 mM P5318 (STM mannitol prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to P5290 (SUC2 dehydration prom.) G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Altered C/N sensing: P5290 (SUC2 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 2 comp. including P6071 4598 Greater tol. to cold (8 C.) P5290 (SUC2 prom.) G1792 AP2 (16-80) 24 G1792 Deletion variant, P25437 4902 Gray leaf color 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 More tol. to drought* swap/chimeric and better recovery variant, 35S from drought treatment* G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Greater res. to swap/chimeric Erysiphe variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25447 4908 Greater res. to swap/chimeric Erysiphe variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Greater res. to swap/chimeric Erysiphe variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Greater res. to swap/chimeric Erysiphe variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Altered C/N sensing: swap/chimeric greater tol. to low variant, 35S nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Greater res. to swap/chimeric Sclerotinia variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Greater res. to swap/chimeric Sclerotinia variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Greater res. to swap/chimeric Sclerotinia variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25438 4903 Late flowering swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Late flowering swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25447 4908 Late flowering swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Late flowering swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Late flowering swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25438 4903 Altered leaf shape swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Altered leaf shape swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25447 4908 Altered leaf shape swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Altered leaf shape swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Altered leaf shape swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25438 4903 Glossy leaves swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Glossy leaves swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25447 4908 Glossy leaves swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Glossy leaves swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Glossy leaves swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25438 4903 Darker green leaf color swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25446 4907 Darker green leaf color swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25447 4908 Darker green leaf color swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25448 4909 Darker green leaf color swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Domain P25445 4906 Darker green leaf color swap/chimeric variant, 35S G1792 AP2 (16-80) 24 G1792 Glucocorticoid P6071 4598 More res. to Botrytis receptor (GR) fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G1792 AP2 (16-80) 24 G1792 Glucocorticoid P6071 4598 More res. to Fusarium receptor (GR) fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 More res. to Botrytis hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 More tol. to drought* hemagglutinin and better recovery (HA) epitope C- from drought terminal tag, 35S treatment* (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Greater res. to hemagglutinin Erysiphe (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Altered C/N sensing: hemagglutinin greater tol. to low (HA) epitope C- nitrogen conditions in terminal tag, 35S C/N sensing assay (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Greater tol. to cold (8 C.) hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Late flowering hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Glossy leaves hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Darker green leaf color hemagglutinin (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Greater res. to hemagglutinin Sclerotinia (HA) epitope C- terminal tag, 35S (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P25118 4853 Altered sugar sensing; hemagglutinin greater tol. to sucrose (HA) epitope C- (determined in 9.4% terminal tag, 35S sucrose) (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P26259 4963 Altered sugar sensing; hemagglutinin greater tol. to sucrose (HA) epitope C- (determined in 9.4% terminal tag, 35S sucrose) (w/ P5486) G1792 AP2 (16-80) 24 G1792 2 comp. P26259 4963 More tol. to drought* hemagglutinin and better recovery (HA) epitope C- from drought terminal tag, 35S treatment* (w/ P5486) G1792 AP2 (16-80) 24 G1792 2-components- P25119 4854 More tol. to drought* supertransformation- and better recovery TAP-C-terminus from drought (w/ P5486) treatment* G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 More tol. to drought* terminal fusion, and better recovery 35S from drought treatment* G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Greater res. to terminal fusion, Erysiphe 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Altered C/N sensing: terminal fusion, greater tol. to low 35S nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Greater tol. to terminal fusion, dehydration 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Greater res. to terminal fusion, Sclerotinia 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Greater tol. to cold (8 C.) terminal fusion, 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Late flowering terminal fusion, 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Altered leaf shape terminal fusion, 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Glossy leaves terminal fusion, 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Darker green leaf color terminal fusion, 35S G1792 AP2 (16-80) 24 G1792 Protein-GFP C P25271 4885 Greater tol. to low terminal fusion, nitrogen conditions 35S G1792 AP2 (16-80) 24 G1792 Point mutation, P25738 4923 Greater res. to 35S Erysiphe G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 Greater res. to 35S Erysiphe G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 Gray leaf color 35S G1792 AP2 (16-80) 24 G1792 Point mutation, P25740 4925 Gray leaf color 35S G1792 AP2 (16-80) 24 G1792 Point mutation, P25741 4926 Gray leaf color 35S G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 Altered C/N sensing: 35S greater tol. to low nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Point mutation, P25740 4925 Altered C/N sensing: 35S greater tol. to low nitrogen conditions in C/N sensing assay G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 Greater tol. to low 35S nitrogen conditions G1792 AP2 (16-80) 24 G1792 Point mutation, P25740 4925 Altered sugar sensing; 35S greater tol. to sucrose (determined in 9.4% sucrose) G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 Greater res. to Botrytis 35S and Erysiphe G1792 AP2 (16-80) 24 G1792 Point mutation, P25739 4924 More tol. to drought* 35S and better recovery from drought treatment* G1792 AP2 (16-80) 24 G1792 Point mutation, P25741 4926 More tol. to drought* 35S and better recovery from drought treatment* G1792 AP2 (16-80) 24 G1792 Direct disease- P27085 5076 Greater res. to inducible prom. Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27086 5077 Greater res. to inducible prom. Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27087 5078 Greater res. to inducible prom. Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27035 5067 Greater res. to inducible prom. Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27201 5080 Greater res. to inducible prom. Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27036 5068 Greater res. to Botrytis inducible prom. and Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27030 5066 Greater res. to Botrytis inducible prom. and Erysiphe fusion G1792 AP2 (16-80) 24 G1792 Direct disease- P27199 5079 Greater res. to Botrytis inducible prom. and Erysiphe fusion G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 More res. to Botrytis P5319 (AS1 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Altered C/N sensing: P5319 (AS1 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Greater tol. to cold (8 C.) P5319 (AS1 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Late flowering P5319 (AS1 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Greater tol. to P5288 (CUT1 dehydration prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Greater res. to P5288 (CUT1 Sclerotinia prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 More res. to Botrytis P5287 (LTP1 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Less sens. to ABA P5284 (RBCS3 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Greater tol. to cold (8 C.) P5284 (RBCS3 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 More tol. to drought* P5284 (RBCS3 and better recovery prom.) from drought treatment* G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Late flowering P5284 (RBCS3 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Late flowering P9002 (RD29A prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Greater tol. to low P9002 (RD29A nitrogen conditions prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Late flowering P5290 (SUC2 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Glossy leaves P5290 (SUC2 prom.) G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Altered C/N sensing: P5290 (SUC2 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G1791 AP2 (10-74) 1172 G1792 2 comp. including P4406 4562 Significantly greater P5297 (PG prom.) soluble solids (Brix) in tomato plants G1791 AP2 (10-74) 1172 G1792 Glucocorticoid P4406 4562 More res. to Botrytis receptor (GR) fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G1791 AP2 (10-74) 1172 G1792 Glucocorticoid P4406 4562 Greater res. to receptor (GR) Sclerotinia fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G1791 AP2 (10-74) 1172 G1792 Knockout not Altered C/N sensing: applicable greater tol. to low nitrogen conditions in C/N sensing assay G1791 AP2 (10-74) 1172 G1792 Knockout not Altered sugar sensing; applicable greater tol. to sucrose (determined in 9.4% sucrose) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5288 (CUT1 Erysiphe prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Late flowering P5288 (CUT1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Glossy leaves P5288 (CUT1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Darker green leaf color P5288 (CUT1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5288 (CUT1 Sclerotinia prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater tol. to P5288 (CUT1 dehydration prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 More res. to Botrytis P5287 (LTP1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5287 (LTP1 Erysiphe prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5287 (LTP1 Sclerotinia prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Late flowering P5287 (LTP1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Glossy leaves P5287 (LTP1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Darker green leaf color P5287 (LTP1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Early flowering P5287 (LTP1 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 More res. to Botrytis P5284 (RBCS3 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5284 (RBCS3 Erysiphe prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5284 (RBCS3 Sclerotinia prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Late flowering P5284 (RBCS3 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Glossy leaves P5284 (RBCS3 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Darker green leaf color P5284 (RBCS3 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater res. to P5290 (SUC2 Sclerotinia prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Less sens. to ABA P5290 (SUC2 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater tol. to 300 mM P5290 (SUC2 mannitol prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater tol. to P5290 (SUC2 dehydration prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Better recovery from P5290 (SUC2 drought treatment* prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Late flowering P5290 (SUC2 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Altered leaf shape P5290 (SUC2 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Glossy leaves P5290 (SUC2 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Darker green leaf color P5290 (SUC2 prom.) G1795 AP2 (11-75) 26 G1792 2 comp. including P6424 4600 Greater tol. to low P5290 (SUC2 nitrogen conditions prom.) G1795 AP2 (11-75) 26 G1792 Direct disease- P26467 5003 Altered C/N sensing: inducible prom. inc. tol. to low nitrogen fusion conditions in C/N sensing assay G1795 AP2 (11-75) 26 G1792 Direct disease- P26402 4973 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26396 4971 Greater res. to inducible prom. Erysiphe fusion G1795 AP2 (11-75) 26 G1792 Direct disease- P26398 4972 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26404 4974 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26409 4978 Greater res. to inducible prom. Sclerotinia fusion G1795 AP2 (11-75) 26 G1792 Direct disease- P26411 4980 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26407 4976 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26412 4981 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26410 4979 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26406 4975 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26408 4977 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26447 4986 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26448 4987 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26460 4997 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26472 5008 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26462 4999 Greater res. to inducible prom. Erysiphe fusion G1795 AP2 (11-75) 26 G1792 Direct disease- P26463 5000 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26465 5001 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26466 5002 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26467 5003 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26468 5004 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26469 5005 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26470 5006 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26471 5007 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26582 5024 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26579 5022 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26477 5011 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26479 5012 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26481 5013 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26442 4982 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26443 4983 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26445 4984 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26580 5023 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26446 4985 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26449 4988 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26450 4989 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26452 4990 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26453 4991 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26454 4992 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26474 5009 Greater res. to inducible prom. Sclerotinia fusion G1795 AP2 (11-75) 26 G1792 Direct disease- P26456 4993 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26457 4994 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26458 4995 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26708 5047 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26459 4996 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26461 4998 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26707 5046 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Direct disease- P26476 5010 Greater res. to inducible prom. Sclerotinia and fusion Erysiphe G1795 AP2 (11-75) 26 G1792 Glucocorticoid P6424 4600 More res. to Botrytis receptor (GR) fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G1795 AP2 (11-75) 26 G1792 Glucocorticoid P6424 4600 Greater res. to receptor (GR) Sclerotinia fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G30 AP2 (16-80) 66 G1792 Const. 35S prom. P893 3993 Glossy darker green leaves G30 AP2 (16-80) 66 G1792 Const. 35S prom. P893 3993 Altered light response; greater shade tol.; lack of shade avoidance phenotype; long cotyledon petioles and hypocotyls G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5318 (STM prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Glossy leaves P5318 (STM prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5318 (STM prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater res. to P5319 (AS1 Erysiphe prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Leaf orientation P5319 (AS1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater res. to P5319 (AS1 Sclerotinia prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5319 (AS1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5319 (AS1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to cold (8 C.) P5288 (CUT1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5288 (CUT1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5288 (CUT1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Leaf orientation P5288 (CUT1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to cold (8 C.) P5287 (LTP1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5287 (LTP1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5287 (LTP1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to low P5287 (LTP1 nitrogen conditions prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 More res. to Botrytis P5284 (RBCS3 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater res. to P5284 (RBCS3 Sclerotinia prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5284 (RBCS3 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5284 (RBCS3 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to NaCl P9002 (RD29A (determined with 150 mM prom.) NaCl) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P9002 (RD29A prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Less sens. to ABA P9002 (RD29A prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Glossy leaves P9002 (RD29A prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P9002 (RD29A prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to cold (8 C.) P5310 (RS1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5310 (RS1 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to cold (8 C.) P5290 (SUC2 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Altered C/N sensing: P5290 (SUC2 greater tol. to low prom.) nitrogen conditions in C/N sensing assay G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Glossy leaves P5290 (SUC2 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to 300 mM P5290 (SUC2 mannitol prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tot. to P5290 (SUC2 dehydration prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Late flowering P5290 (SUC2 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Darker green leaf color P5290 (SUC2 prom.) G30 AP2 (16-80) 66 G1792 2 comp. including P3852 4531 Greater tol. to low P5290 (SUC2 nitrogen conditions prom.) G30 AP2 (16-80) 66 G1792 Knockout not C/N sensing: greater applicable sens. G30 AP2 (16-80) 66 G1792 Glucocorticoid P3852 4531 More res. to Botrytis receptor (GR) fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G30 AP2 (16-80) 66 G1792 Glucocorticoid P25086 4849 Greater res. to receptor (GR) Sclerotinia fusion (dexamethasone- inducible), 35S prom. G30 AP2 (16-80) 66 G1792 Glucocorticoid P3852 4531 Greater res. to receptor (GR) Sclerotinia fusion (dexamethasone- inducible), 35S prom. (w/ P5486) G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Greater tol. to cold (8 C.) G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 More tol. to drought* and better recovery from drought treatment* G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Greater res. to Erysiphe G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Greater tol. to NaCl (determined with 150 mM NaCl) G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Greater sens. to heat (32 C.) G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Altered leaf shape G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Glossy leaves G3518 AP2 (13-77) 1982 G1792 Const. 35S prom. P21404 4809 Darker green leaf color G3519 AP2 (13-77) 1984 G1792 Const. 35S prom. P21405 4810 Greater res. to Erysiphe G3519 AP2 (13-77) 1984 G1792 Const. 35S prom. P21405 4810 Late flowering G3519 AP2 (13-77) 1984 G1792 Const. 35S prom. P21405 4810 Altered leaf shape G3519 AP2 (13-77) 1984 G1792 Const. 35S prom. P21405 4810 Glossy leaves G3519 AP2 (13-77) 1984 G1792 Const. 35S prom. P21405 4810 Darker green leaf color G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Greater res. to Erysiphe G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Greater res. to Sclerotinia G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Late flowering G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Altered leaf shape G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Glossy leaves G3520 AP2 (14-78) 1986 G1792 Const. 35S prom. P21406 4811 Darker green leaf color G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 More tol. to drought* and better recovery from drought treatment* G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 Greater res. to Erysiphe G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 Less sens. to ABA G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 Greater tol. to 300 mM mannitol G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 Greater tol. to cold (8 C.) G3380 AP2 (18-82) 1846 G1792 Const. 35S prom. P21460 4812 Late flowering G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Greater tol. to cold (8 C.) G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Greater res. to Erysiphe G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Greater res. to Sclerotinia G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 More tol. to drought* and better recovery from drought treatment* G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Late flowering G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Darker green leaf color G3381 AP2 (14-78) 1848 G1792 Const. 35S prom. P21461 4813 Greater tol. to hyperosmotic stress; more tol. to 300 mM mannitol or to NaCl (determined with 150 mM NaCl) G3383 AP2 (9-73) 1850 G1792 Const. 35S prom. P23523 4844 Greater tol. to cold (8 C.) G3383 AP2 (9-73) 1850 G1792 Const. 35S prom. P23523 4844 Greater tol. to dehydration G3383 AP2 (9-73) 1850 G1792 Const. 35S prom. P23523 4844 Greater tol. to 300 mM mannitol G3515 AP2 (11-75) 1976 G1792 Const. 35S prom. P21401 4806 More tol. to drought* and better recovery from drought treatment* G3515 AP2 (11-75) 1976 G1792 Const. 35S prom. P21401 4806 More root hair G3515 AP2 (11-75) 1976 G1792 Const. 35S prom. P21401 4806 More root mass G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Greater tol. to cold (8 C.) G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 More tol. to drought* and better recovery from drought treatment* G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Less sens. to ABA G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Greater tol. to dehydration G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Greater tol. to NaCl (determined with 150 mM NaCl) G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Inflorescence: decreased apical dominance G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Greater res. to Erysiphe G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Late flowering G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Altered leaf shape G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Darker green leaf color G3737 AP2 (8-72) 2104 G1792 Const. 35S prom. P25089 4850 Glossy leaves G3516 AP2 (6-70) 1978 G1792 Const. 35S prom. P21402 4807 Greater tol. to cold (8 C.) G3516 AP2 (6-70) 1978 G1792 Const. 35S prom. P21402 4807 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G3517 AP2 (13-77) 1980 G1792 Const. 35S prom. P21403 4808 Greater res. to Erysiphe G3517 AP2 (13-77) 1980 G1792 Const. 35S prom. P21403 4808 More res. to Botrytis G3517 AP2 (13-77) 1980 G1792 Const. 35S prom. P21403 4808 Greater tol. to cold (8 C..) G3517 AP2 (13-77) 1980 G1792 Const. 35S prom. P21403 4808 Greater tol. to heat (32 C.) G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Greater res. to Erysiphe G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Greater tol. to 300 mM mannitol G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Greater tol. to cold (8 C.) G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Greater tol. to dehydration G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Less sens. to ABA G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Altered inflorescence: decreased apical dominance G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Late flowering G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Altered leaf shape G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Glossy leaves G3739 AP2 (13-77) 2106 G1792 Const. 35S prom. P25090 4851 Darker green leaf color G3794 AP2 (6-70) 2132 G1792 Const. 35S prom. P25092 4852 Greater tol. to cold (8 C.) G3794 AP2 (6-70) 2132 G1792 Const. 35S prom. P25092 4852 Greater tol. to dehydration G3794 AP2 (6-70) 2132 G1792 Const. 35S prom. P25092 4852 Altered leaf shape G1945 AT-hook 28 G1945 Const. 35S prom. P2085 4343 Late flowering (56-64, 64-214) G1945 AT-hook 28 G1945 Const. 35S prom. P2085 4343 Altered leaf shape; (56-64, 64-214) large, broad, serrated, curling leaves G1945 AT-hook 28 G1945 Const. 35S prom. P2085 4343 Greater biomass (56-64, 64-214) G2155 AT-hook 1422 G1945 Const. 35S prom. P1742 4246 Late flowering (24-32, 32-180) G2155 AT-hook 1422 G1945 Const. 35S prom. P1742 4246 Greater biomass; very (24-32, 32-180) large plants G2155 AT-hook 1422 G1945 Const. 35S prom. P1742 4246 Late senescence (24-32, 32-180) G2155 AT-hook 1422 G1945 Const. 35S prom. P1742 4246 Increase in seed (24-32, 32-180) glucosinolate M39497 in T2 lines G2155 AT-hook 1422 G1945 Const. 35S prom. P1742 4246 Altered light response; (24-32, 32-180) greater shade tol.; lack of shade avoidance phenotype G3408 AT-hook 1878 G1945 Const. 35S prom. P21246 4755 Late developing, late (82-90, 90-247) flowering G3408 AT-hook 1878 G1945 Const. 35S prom. P21246 4755 Altered leaf shape; (82-90, 90-247) large, broad, curling leaves G3408 AT-hook 1878 G1945 Const. 35S prom. P21246 4755 Greater biomass (82-90, 90-247) G3408 AT-hook 1878 G1945 Const. 35S prom. P21246 4755 Greater tol. to drought* (82-90, 90-247) G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Altered sugar sensing; (5-50) greater tol. to sucrose (determined in 9.4% sucrose) G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Greater tol. to (5-50) dehydration G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Better recovery from (5-50) drought treatment* G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Late developing (5-50) G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 More root mass (5-50) G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Altered light response; (5-50) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; upright leaves, longer hypocotyls, elongated petioles G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Altered C/N sensing: (5-50) greater tol. to low nitrogen conditions in C/N sensing assay G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Greater tol. to cold (8 C.) (5-50) G1988 Z-CO-like 30 G1988 Const. 35S prom. P2499 4407 Improved yield (5-50) G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Greater tol. to cold (8 C.) (6-51) G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Long petiole (6-51) G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Altered light response; (6-51) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; upright leaves, longer hypocotyls, elongated and upright petioles G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Altered C/N sensing: (6-51) greater tol. to low nitrogen conditions in C/N sensing assay G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Long hypocotyls (6-51) G4004 Z-CO-like 2252 G1988 Const. 35S prom. P26748 5050 Late developing (6-51) G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Long petiole (6-51) G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Altered light response; (6-51) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; upright leaves, light green, elongated and upright petioles G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Late developing (6-51) G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Altered C/N sensing: (6-51) greater tol. to low nitrogen conditions in C/N sensing assay G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Some lines have (6-51) decreased tol. to cold (8 C.), but more lines are more tol to cold (8 C.) G4005 Z-CO-like 2254 G1988 Const. 35S prom. P26749 5051 Altered sugar sensing; (6-51) some lines have decreased tol. to 9.4% sucrose, but more lines are more tol to 9.4% sucrose G4007 Z-CO-like 2256 G1988 — — n/d (5-50) G4011 Z-CO-like 2260 G1988 Const. 35S prom P27405 5084 Altered sugar sensing; (8-49) greater tol. to sucrose (determined in 9.4% sucrose) G4011 Z-CO-like 2260 G1988 Const. 35S prom P27405 5084 More tol. to cold (8 C.) (8-49) G4011 Z-CO-like 2260 G1988 Const. 35S prom P27405 5084 Altered C/N sensing: (8-49) greater tol. to low nitrogen conditions in C/N sensing assay G4012 Z-CO-like 2262 G1988 Const. 35S prom P27406 5085 Altered light response; (15-56) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; upright leaves, longer hypocotyls, elongated and upright petioles G4012 Z-CO-like 2262 G1988 Const. 35S prom P27406 5085 Late flowering (15-56) G4012 Z-CO-like 2262 G1988 Const. 35S prom P27406 5085 Altered sugar sensing; (15-56) greater tol. to sucrose (determined in 9.4% sucrose) G4012 Z-CO-like 2262 G1988 Const. 35S prom P27406 5085 More tol. to cold (8 C.) (15-56) G4012 Z-CO-like 2262 G1988 Const. 35S prom P27406 5085 Altered C/N sensing: (15-56) greater tol. to low nitrogen conditions in C/N sensing assay G4298 Z-CO-like 2350 G1988 — — n/d (15-56) G4009 Z-CO-like 2258 G1988 — — n/d (6-51) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Long petiole (9-54) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Long hypocotyls (9-54) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Altered light response; (9-54) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; upright pale leaves, longer hypocotyls G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Late developing (9-54) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Altered sugar sensing; (9-54) greater tol. to sucrose (determined in 9.4% sucrose) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 More tol. to cold (8 C.) (9-54) G4299 Z-CO-like 2352 G1988 Const. 35S prom. P27428 5086 Altered C/N sensing: (9-54) greater tol. to low nitrogen conditions in C/N sensing assay G4000 Z-CO-like 2250 G1988 Const. 35S prom. P27404 5083 Altered light response; (20-61) greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; narrow upright leaves, longer hypocotyls G4000 Z-CO-like 2250 G1988 Const. 35S prom. P27404 5083 Late developing (20-61) G4000 Z-CO-like 2250 G1988 Const. 35S prom. P27404 5083 Some lines more sens. (20-61) to cold (8 C.) G4000 Z-CO-like 2250 G1988 Const. 35S prom. P27404 5083 Altered sugar sensing; (20-61) greater tol. to sucrose (determined in 9.4% sucrose) G4000 Z-CO-like 2250 G1988 Const. 35S prom. P27404 5083 Altered C/N sensing: (20-61) greater tol. to low nitrogen conditions in C/N sensing assay G4297 Z-CO-like 2348 G1988 — — n/d (14-55) G2053 NAC (6-152) 1336 G2053 Const. 35S prom. P2032 4323 Early flowering G2053 NAC (6-152) 1336 G2053 Const. 35S prom. P2032 4323 More root growth under hyperosmotic stress with PEG G2053 NAC (6-152) 1336 G2053 Const. 35S prom. P2032 4323 More tol. to drought* and better recovery from drought treatment* G2053 NAC (6-152) 1336 G2053 Const. 35S prom. P2032 4323 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G515 NAC (6-149) 410 G2053 Const. 35S prom. P2791 4469 Lethal when constitutively overexpressed G516 NAC (6-141) 412 G2053 Const. 35S prom. P279 3882 Greater tol. to cold (8 C.) G516 NAC (6-141) 412 G2053 Const. 35S prom. P279 3882 Greater tol. to hyperosmotic stress (300 mM mannitol) G517 NAC (6-153) 414 G2053 — — n/d G2999 ZF-HB (80-133, 1794 G2999 Const. 35S prom. P15277 4692 More root hair 198-261) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 More root hair 198-261) P6506 (35S prom.) G2999 ZF-HB (80-133, 1794 G2999 Const. 35S prom. P15277 4692 Greater tol. to cold (8 C.) 198-261) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Early flowering 198-261) P6506 (35S prom.) G2999 ZF-HB (80-133, 1794 G2999 Const. 35S prom. P15277 4692 Decreased root mass 198-261) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater tol. to 198-261) P6506 (35S prom.) hyperosmotic stress; more tol. to 9.4% sucrose or 150 mM NaCl G2999 ZF-HB (80-133, 1794 G2999 Const. 35S prom. P15277 4692 More tol. to drought* 198-261) and better recovery from drought treatment* G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 More tol. to drought* 198-261) P6506 (35S prom.) and better recovery from drought treatment* G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater tol. to 198-261) P5319 (AS1 dehydration prom.) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater tol. to 198-261) P5288 (CUT1 dehydration prom.) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Less sens. to ABA 198-261) P5284 (RBCS3 prom.) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater tol. to 300 mM 198-261) P5284 (RBCS3 mannitol or to NaCl prom.) (determined with 150 mM NaCl) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 More tol. to drought* 198-261) P5284 (RBCS3 and better recovery prom.) from drought treatment* G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater tol. to cold (8 C.) 198-261) P5318 (STM prom.) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Greater seedling vigor 198-261) P5290 (SUC2 prom.) G2999 ZF-HB (80-133, 1794 G2999 2 comp. including P8587 4612 Altered sugar sensing; 198-261) P5290 (SUC2 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Less sens. to ABA 198-261) (Super Active), 35S G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Early flowering 198-261) (Super Active), 35S G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Greater tol. to heat (32 C.) 198-261) (Super Active), 35S G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Greater tol. to 198-261) (Super Active), dehydration 35S G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Greater tol. to 198-261) (Super Active), hyperosmotic stress; 35S more tol. to 9.4% sucrose, 300 mM mannitol or to 150 mM NaCl G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Greater seedling vigor 198-261) (Super Active), 35S G2999 ZF-HB (80-133, 1794 G2999 GAL4 N-term P25173 4862 Greater tol. to NaCl 198-261) (Super Active), (determined with 150 mM 35S NaCl) G2999 ZF-HB (80-133, 1794 G2999 GAL4 C-term P25147 4855 Early flowering 198-261) (Super Active), 35S G2999 ZF-HB (80-133, 1794 G2999 Point mutation, P25736 4922 Less sens. to ABA 198-261) 35S G2999 ZF-HB (80-133, 1794 G2999 Point mutation, P25736 4922 More tol. to 198-261) 35S hyperosmotic stress; more tol. to 9.4% sucrose or 300 mM mannitol G2999 ZF-HB (80-133, 1794 G2999 Point mutation, P25736 4922 Greater tol. to 198-261) 35S dehydration G2999 ZF-HB (80-133, 1794 G2999 Point mutation, P25736 4922 Better recovery from 198-261) 35S drought treatment* G2989 ZF-HB (50-105, 1776 G2999 Const. 35S prom. P2425 4386 Greater tol. to 192-255) dehydration G2989 ZF-HB (50-105, 1776 G2999 Const. 35S prom. P2425 4386 More tol. to drought* 192-255) and better recovery from drought treatment* G2989 ZF-HB (50-105, 1776 G2999 Const. 35S prom. P2425 4386 Greater tol. to cold (8 C.) 192-255) G2990 ZF-HB (54-109, 1778 G2999 Const. 35S prom. P2426 4387 Altered C/N sensing: 200-263) greater tol. to low nitrogen conditions in C/N sensing assay G2990 ZF-HB (54-109, 1778 G2999 Const. 35S prom. P2426 4387 Greater tol. to cold (8 C.) 200-263) G2990 ZF-HB (54-109, 1778 G2999 Const. 35S prom. P2426 4387 More tol. to drought* 200-263) and better recovery from drought treatment* G2990 ZF-HB (54-109, 1778 G2999 Const. 35S prom. P2426 4387 Less sens. to ABA 200-263) G2990 ZF-HB (54-109, 1778 G2999 Knockout not Altered sugar sensing; 200-263) applicable greater tol. to sucrose (determined in 9.4% sucrose) G2991 ZF-HB (54-109, 1780 G2999 Const. 35S prom. P2423 4384 More root mass 179-242) G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Altered silique 156-219) development G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Greater tol. to NaCl 156-219) (determined with 150 mM NaCl) G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Less sens. to ABA 156-219) G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Altered C/N sensing: 156-219) greater sens. to low nitrogen conditions in C/N sensing assay G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Fewer lateral roots 156-219) G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Early flowering 156-219) G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 More tol. to drought* 156-219) and better recovery from drought treatment* G2992 ZF-HB (29-84, 1782 G2999 Const. 35S prom. P2427 4388 Smaller plants 156-219) G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Altered light response; 222-285) greater shade tol.; lack of shade avoidance phenotype; elongated hypocotyl and leaves in a vertical orientation G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Altered root branching 222-285) G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Late flowering 222-285) G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Lack of apical 222-285) dominance G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Greater sens. to 300 mM 222-285) mannitol or 150 mM NaCl G2993 ZF-HB (85-138, 1784 G2999 Const. 35S prom. P13792 4640 Inc. sens. to cold (8 C.) 222-285) G2994 ZF-HB (88-141, 1786 G2999 Const. 35S prom. P2434 4390 Decreased root mass 218-281) G2996 ZF-HB (73-126, 1788 G2999 Const. 35S prom. P2424 4385 Early flowering 191-254) G2996 ZF-HB (73-126, 1788 G2999 Const. 35S prom. P2424 4385 Decreased root mass 191-254) G2996 ZF-HB (73-126, 1788 G2999 Const. 35S prom. P2424 4385 Altered silique 191-254) development G2996 ZF-HB (73-126, 1788 G2999 Const. 35S prom. P2424 4385 Altered sugar sensing; 191-254) greater tol. to sucrose (determined in 9.4% sucrose) G2996 ZF-HB (73-126, 1788 G2999 Const. 35S prom. P2424 4385 Inc. sens. to mannitol 191-254) in root growth inhibition assays, (no secondary root growth) indicating this gene influences osmotic stress response G2997 ZF-HB (47-100, 1790 G2999 Const. 35S prom. P15364 4698 Greater tol. to cold (8 C.) 157-220) G2997 ZF-HB (47-100, 1790 G2999 Const. 35S prom. P15364 4698 Greater sens. to heat 157-220) (32 C.) G2997 ZF-HB (47-100, 1790 G2999 Const. 35S prom. P15364 4698 Altered silique 157-220) development G2998 ZF-HB (74-127, 1792 G2999 Const. 35S prom. P2431 4389 Wild-type in plate- 240-303) based physiological assays, more sens. to drought treatment* G2998 ZF-HB (74-127, 1792 G2999 Const. 35S prom. P2431 4389 More tol, to NaCl 240-303) (determined in 150 mM NaCl) G2998 ZF-HB (74-127, 1792 G2999 Const. 35S prom. P2431 4389 Late flowering 240-303) G3000 ZF-HB (58-111, 1796 G2999 Const. 35S prom. P23554 4848 Early flowering 181-244) G3002 ZF-HB (5-53, 1798 G2999 Const. 35S prom. P15113 4680 Greater tol. to cold (8 C.) 106-168) G3002 ZF-HB (5-53, 1798 G2999 Const. 35S prom. P15113 4680 Early flowering 106-168) G3003 Z-C2H2 1800 G2999 Const. 35S prom. P3291 4489 Late flowering (131-280) G3674 ZF-HB (61-114, 2060 G2999 Const. 35S prom. P25158 4856 Early flowering 226-289) G3674 ZF-HB (61-114, 2060 G2999 Const. 35S prom. P25158 4856 Early flowering 226-289) G3683 ZF-HB (72-125, 2068 G2999 Const. 35S prom. P25165 4859 Early flowering 193-256) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Greater tol. to cold (8 C.) 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Less sens. to ABA 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 More root hair 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Greater seedling vigor 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Better recovery from 172-235) drought treatment* G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Late flowering 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Early flowering 172-235) G3685 ZF-HB (43-95, 2070 G2999 Const. 35S prom. P25166 4860 Darker green leaf color 172-235) G3686 ZF-HB (38-88, 2072 G2999 Const. 35S prom. P25167 4861 More tol. to drought* 159-222) and better recovery from drought treatment* G3686 ZF-HB (38-88, 2072 G2999 Const. 35S prom. P25167 4861 Early flowering 159-222) G3686 ZF-HB (38-88, 2072 G2999 Const. 35S prom. P25167 4861 Greater tol. to cold (8 C.) 159-222) G3690 ZF-HB 2074 G2999 Const. 35S prom. P25407 4898 Late flowering (161-213, 318-381) G3690 ZF-HB 2074 G2999 Const. 35S prom. P25407 4898 Greater tol. to heat (32 C.) (161-213, 318-381) G3690 ZF-HB 2074 G2999 Const. 35S prom. P25407 4898 Altered leaf shape (161-213, 318-381) G3690 ZF-HB 2074 G2999 Const. 35S prom. P25407 4898 Greater tol. to 300 mM (161-213, mannitol 318-381) G3676 ZF-HB (40-89, 2062 G2999 Const. 35S prom. P25159 4857 Greater tol. to 162-225) dehydration G3676 ZF-HB (40-89, 2062 G2999 Const. 35S prom. P25159 4857 Early flowering 162-225) G3676 ZF-HB (40-89, 2062 G2999 Const. 35S prom. P25159 4857 Greater tol. to NaCl 162-225) (determined with 150 mM NaCl) G3680 ZF-HB (34-89, 2064 G2999 Const. 35S prom. P25405 4897 Early flowering 222-285) G3681 ZF-HB (22-77, 2066 G2999 Const. 35S prom. P25163 4858 Early flowering 208-271) G3681 ZF-HB (22-77, 2066 G2999 Const. 35S prom. P25163 4858 Greater tol. to NaCl 208-271) (determined with 150 mM NaCl) G3086 HLH/MYC 1836 G3086 Const. 35S prom. P15046 4668 Greater tol. to (307-365) dehydration G3086 HLH/MYC 1836 G3086 Const. 35S prom. P15046 4668 Greater tol. to cold (8 C.) (307-365) G3086 HLH/MYC 1836 G3086 Const. 35S prom. P15046 4668 Greater tol. to heat (32 C.) (307-365) G3086 HLH/MYC 1836 G3086 Const. 35S prom. P15046 4668 Greater tol. to NaCl (307-365) (determined with 150 mM NaCl) G3086 HLH/MYC 1836 G3086 Const. 35S prom. P15046 4668 More tol. to drought* (307-365) and better recovery from drought treatment* G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 More tol. to drought* (307-365) P6506 (35S prom.) and better recovery from drought treatment* G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 Altered sugar sensing; (307-365) P5284 (RBCS3 greater tol. to sucrose prom.) (determined in 9.4% sucrose) G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 Early flowering (307-365) P5310 (RS1 prom.) G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 Greater tol. to (307-365) P5318 (STM dehydration prom.) G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 More tol. to drought* (307-365) P5318 (STM and better recovery prom.) from drought treatment* G3086 HLH/MYC 1836 G3086 2 comp. including P8242 4610 Early flowering (307-365) P5290 (SUC2 prom.) G3086 HLH/MYC 1836 G3086 GAL4 N-term P25662 4918 Greater tol. to heat (32 C.) (307-365) (Super Active), 35S G3086 HLH/MYC 1836 G3086 GAL4 N-term P25662 4918 Greater tol. to 300 mM (307-365) (Super Active), mannitol 35S G3086 HLH/MYC 1836 G3086 GAL4 C-term P25660 4916 Less sens. to ABA (307-365) (Super Active), 35S G3086 HLH/MYC 1836 G3086 GAL4 C-term P25660 4916 Greater tol. to 300 mM (307-365) (Super Active), mannitol 35S G3086 HLH/MYC 1836 G3086 GAL4 C-term P25660 4916 Early flowering (307-365) (Super Active), 35S G3086 HLH/MYC 1836 G3086 GAL4 C-term P25660 4916 Greater tol. to heat (32 C.) (307-365) (Super Active), 35S G3086 HLH/MYC 1836 G3086 Knockout not Late flowering (307-365) applicable G1134 HLH/MYC 834 G3086 Const. 35S prom. P467 3942 Early flowering (187-245) G1134 HLH/MYC 834 G3086 Const. 35S prom. P467 3942 Less sens. to ABA (187-245) G1134 HLH/MYC 834 G3086 Const. 35S prom. P467 3942 More root mass (187-245) G1134 HLH/MYC 834 G3086 Const. 35S prom. P467 3942 Altered response to (187-245) ethylene: longer hypocotyls and lack of apical hook G1134 HLH/MYC 834 G3086 Const. 35S prom. P467 3942 Wrinkled, sickle- (187-245) shaped siliques G2555 HLH/MYC 1570 G3086 Const. 35S prom. P2069 4339 Greater tol. to heat (32 C.) (184-242) G2555 HLH/MYC 1570 G3086 Const. 35S prom. P2069 4339 Greater tol. to cold (8 C.) (184-242) G2555 HLH/MYC 1570 G3086 Const. 35S prom. P2069 4339 Altered light response (184-242) and/or shade tol.; constitutive photomorphogenesis G2555 HLH/MYC 1570 G3086 Const. 35S prom. P2069 4339 Inc. susceptibility to (184-242) Botrytis G2766 HLH/MYC 1676 G3086 Const. 35S prom. P2532 4417 Greater tol. to cold (8 C.) (234-292) G2766 HLH/MYC 1676 G3086 Const. 35S prom. P2532 4417 Greater sens. to heat (234-292) (32 C.) G2791 HLH/MYC 1698 G3086 Const. 35S prom. P2531 4416 Early flowering (102-159) G793 HLH/MYC 606 G3086 Const. 35S prom. P131 3838 Greater tol. to (147-204) dehydration G793 HLH/MYC 606 G3086 Const. 35S prom. P131 3838 Greater res. to (147-204) Sclerotinia G3765 HLH/MYC 2120 G3086 Const. 35S prom. P25241 4878 More tol. to drought* (147-205) and better recovery from drought treatment* G3765 HLH/MYC 2120 G3086 Const. 35S prom. P25241 4878 Early flowering (147-205) G3765 HLH/MYC 2120 G3086 Const. 35S prom. P25241 4878 Less sens. to ABA (147-205) G3766 HLH/MYC 2122 G3086 Const. 35S prom. P25242 4879 Early flowering (35-93) G3766 HLH/MYC 2122 G3086 Const. 35S prom. P25242 4879 Less sens. to ABA (35-93) G3766 HLH/MYC 2122 G3086 Const. 35S prom. P25242 4879 Greater tol. to cold (8 C.) (35-93) G3767 HLH/MYC 2124 G3086 Const. 35S prom. P25243 4880 Early flowering (146-204) G3767 HLH/MYC 2124 G3086 Const. 35S prom. P25243 4880 Greater tol. to (146-204) dehydration G3767 HLH/MYC 2124 G3086 Const. 35S prom. P25243 4880 Less sens. to ABA (146-204) G3767 HLH/MYC 2124 G3086 Const. 35S prom. P25243 4880 More root mass (146-204) G3768 HLH/MYC 2126 G3086 Const. 35S prom. P25244 4881 Early flowering (190-248) G3768 HLH/MYC 2126 G3086 Const. 35S prom. P25244 4881 Less sens. to ABA (190-248) G3769 HLH/MYC 2128 G3086 Const. 35S prom. P25245 4882 Early flowering (240-298) G3769 HLH/MYC 2128 G3086 Const. 35S prom. P25245 4882 Less sens. to ABA (240-298) G3771 HLH/MYC 2130 G3086 Const. 35S prom. P25246 4883 Early flowering (84-142) G3771 HLH/MYC 2130 G3086 Const. 35S prom. P25246 4883 Greater tol. to (84-142) dehydration G3771 HLH/MYC 2130 G3086 Const. 35S prom. P25246 4883 More tol. to drought* (84-142) and better recovery from drought treatment* G3742 HLH/MYC 2108 G3086 Const. 35S prom. P25661 4917 Greater tol. to cold (8 C.) (199-257) G3744 HLH/MYC 2110 G3086 Const. 35S prom. P25370 4894 More tol. to drought* (71-129) and better recovery from drought treatment* G3744 HLH/MYC 2110 G3086 Const. 35S prom. P25370 4894 Less sens. to ABA (71-129) G3744 HLH/MYC 2110 G3086 Const. 35S prom. P25370 4894 Greater biomass (71-129) G3744 HLH/MYC 2110 G3086 Const. 35S prom. P25370 4894 Altered sugar sensing; (71-129) greater tol. to sucrose (determined in 9.4% sucrose) G3744 HLH/MYC 2110 G3086 Const. 35S prom. P25370 4894 Late flowering (71-129) G3746 HLH/MYC 2112 G3086 Const. 35S prom. P25230 4876 Early developing (312-370) G3750 HLH/MYC 2114 G3086 Const. 35S prom. P25233 4877 Greater tol. to heat (32 C.) (136-193) G3750 HLH/MYC 2114 G3086 Const. 35S prom. P25233 4877 Early flowering (136-193) G3750 HLH/MYC 2114 G3086 Const. 35S prom. P25233 4877 Greater tol. to 300 mM (136-193) mannitol G3750 HLH/MYC 2114 G3086 Const. 35S prom. P25233 4877 Greater tol. to (136-193) dehydration G3750 HLH/MYC 2114 G3086 Const. 35S prom. P25233 4877 Less sens. to ABA (136-193) G3755 HLH/MYC 2116 G3086 Const. 35S prom. P25426 4900 Greater tol. to cold (8 C.) (97-155) G3755 HLH/MYC 2116 G3086 Const. 35S prom. P25426 4900 Late flowering (97-155) G3755 HLH/MYC 2116 G3086 Const. 35S prom. P25426 4900 Early flowering (97-155) G3760 HLH/MYC 2118 G3086 Const. 35S prom. P25360 4892 Greater tol. to cold (8 C.) (243-300) G3760 HLH/MYC 2118 G3086 Const. 35S prom. P25360 4892 Early flowering (243-300) G3760 HLH/MYC 2118 G3086 Const. 35S prom. P25360 4892 Less sens. to ABA (243-300) G3760 HLH/MYC 2118 G3086 Const. 35S prom. P25360 4892 Greater tol. to NaCl (243-300) (determined with 150 mM NaCl) G2 AP2 (129-195, 32 Const. 35S prom. P13435 4625 Late flowering 221-288) G3 AP2 (28-95) 34 Const. 35S prom. P1094 4055 Small plant G3 AP2 (28-95) 34 Const. 35S prom. P1094 4055 More sensitive to heat in a growth assay G3 AP2 (28-95) 34 2 comp. including P3375 4508 Significantly greater P5284 (RBCS3 lycopene in tomato prom.) plants G4 AP2 (121-183) 36 Const. 35S prom. P163 3848 Greater resistance to Botrytis G8 AP2 (151-217, 42 Const. 35S prom. P1218 4068 Late flowering 243-293) G8 AP2 (151-217, 42 Const. 35S prom. P1218 4068 Altered C/N sensing: 243-293) accumulated more anthocyanin in C/N sensing assay G12 AP2 (27-94) 46 Knockout not Inc. sens. to ACC applicable G12 AP2 (27-94) 46 Const. 35S prom. P1216 4067 Inc. leaf and hypocotyl necrosis; knockout seedlings germinated in the dark on 1- aminocyclopropane-1- carboxylic acid- containing media were more stunted than controls G15 AP2 (281-357, 48 Const. 35S prom. P15341 4694 Altered flower 383-451) morphology; partial conversion of stamens into petalloid organs, floral organs enlarged and fertility was poor, with few siliques G19 AP2 (76-143) 50 Const. 35S prom. P1 3792 Greater resistance to Erysiphe; repressed by methyl jasmonate and induced by ACC G20 AP2 (68-144) 52 Const. 35S prom. P171 3852 Reduced size G21 AP2 (97-164) 54 Const. 35S prom. P1576 4193 More tol. to high salt G24 AP2 (25-92) 58 Const. 35S prom. P969 4012 Altered necrosis; reduced size and necrotic patches G24 AP2 (25-92) 58 Const. 35S prom. P969 4012 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G24 AP2 (25-92) 58 Const. 35S prom. P969 4012 Smaller plant G24 AP2 (25-92) 58 Const. 35S prom. P969 4012 Altered light response; greater shade tol.; lack of shade avoidance phenotype G24 AP2 (25-92) 58 2 comp. including P4776 4589 Significantly greater P5319 (AS1 tomato plant volume prom.) G24 AP2 (25-92) 58 2 comp. including P4776 4589 Significantly greater P5324 (Cru prom.) tomato plant volume G25 AP2 (47-114) 60 Const. 35S prom. P804 3976 Fewer trichomes at seedling stage G25 AP2 (47-114) 60 Const. 35S prom. P804 3976 Expression induced by Fusarium infection G26 AP2 (67-134) 62 Const. 35S prom. P807 3978 Decreased germination and growth on 5% glucose medium G27 AP2 (37-104) 64 Const. 35S prom. P173 3853 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G27 AP2 (37-104) 64 Const. 35S prom. P173 3853 Late flowering G27 AP2 (37-104) 64 Const. 35S prom. P173 3853 Delayed senescence G27 AP2 (37-104) 64 Const. 35S prom. P173 3853 Darker green G27 AP2 (37-104) 64 Const. 35S prom. P173 3853 Smaller plant G32 AP2 (17-84) 68 Const. 35S prom. P1379 4128 Curled darker green, glossy leaves G38 AP2 (76-143) 70 Const. 35S prom. P179 3855 Reduced germination on glucose medium G38 AP2 (76-143) 70 Const. 35S prom. P179 3855 Altered light response; greater shade tol.; lack of shade avoidance phenotype G43 AP2 (104-172) 72 Const. 35S prom. P181 3856 Decreased germination and growth on glucose medium G44 AP2 (85-154) 74 Const. 35S prom. P182 3857 Altered light response; greater shade tol.; lack of shade avoidance phenotype G46 AP2 (107-175) 78 Const. 35S prom. P1090 4052 Greater biomass; overexpressors were larger, developed more rapidly, and yielded an greater quantity of seed compared to wild type controls G46 AP2 (107-175) 78 Const. 35S prom. P1090 4052 Inc. tol. to drought* G129 MADS (18-73) 80 Const. 35S prom. P3315 4496 Early flowering G129 MADS (18-73) 80 Const. 35S prom. P3315 4496 Altered leaf shape; up- curled leaves G129 MADS (18-73) 80 Const. 35S prom. P3315 4496 Homeotic transformation; transformations of sepals into carpelloid structures and petals into stamens G131 MADS (1-57) 82 Const. 35S prom. P15154 4682 Smaller plants G131 MADS (1-57) 82 Const. 35S prom. P15154 4682 Early flowering G133 MADS (1-57) 84 Const. 35S prom. P13813 4645 Early flowering G134 MADS (1-57) 86 Const. 35S prom. P2102 4347 Homeotic transformation; conversion of sepals to petals G134 MADS (1-57) 86 Const. 35S prom. P2102 4347 Greater sens. to cold (8 C.) G135 MADS (1-57) 88 Const. 35S prom. P2103 4348 Curled leaves G135 MADS (1-57) 88 Const. 35S prom. P2103 4348 Altered inflorescence determinacy; terminal flowers G135 MADS (1-57) 88 Const. 35S prom. P2103 4348 Loss of flower determinacy G135 MADS (1-57) 88 Const. 35S prom. P2103 4348 Early flowering G136 MADS (18-74) 90 Const. 35S prom. P2104 4349 Altered flower development; tiny petals G136 MADS (18-74) 90 Const. 35S prom. P2104 4349 Early flowering G136 MADS (18-74) 90 Const. 35S prom. P2104 4349 Small, upward curling leaves G136 MADS (18-74) 90 Const. 35S prom. P2104 4349 Smaller plant G137 MADS (1-57) 92 Const. 35S prom. P2105 4350 Early flowering G137 MADS (1-57) 92 Const. 35S prom. P2105 4350 Terminal flower formation G137 MADS (1-57) 92 Const. 35S prom. P2105 4350 Leaf curling G138 MADS (1-57) 94 Const. 35S prom. P2106 4351 Early flowering G140 MADS (16-72) 96 Const. 35S prom. P3310 4494 Homeotic transformation; sepals were converted towards a carpelloid identity G140 MADS (16-72) 96 Const. 35S prom. P3310 4494 Early flowering G142 MADS (2-57) 98 Const. 35S prom. P2109 4352 Early flowering G145 MADS (1-57) 100 Const. 35S prom. P15453 4704 Early flowering G145 MADS (1-57) 100 Const. 35S prom. P15453 4704 Terminal flowers G146 MADS (1-57) 102 Const. 35S prom. P2111 4353 Better growth in low nitrogen media G146 MADS (1-57) 102 Const. 35S prom. P2111 4353 Altered C:N sensing: reduced anthocyanin production on high sucrose/low nitrogen G146 MADS (1-57) 102 Const. 35S prom. P2111 4353 Early flowering G147 MADS (2-57) 104 Const. 35S prom. P895 3995 Early flowering G148 MADS (1-57) 106 Const. 35S prom. P13734 4636 Early flowering G148 MADS (1-57) 106 Const. 35S prom. P13734 4636 Terminal flower; inflorescences that often terminated in a cluster of siliques G151 MADS (2-57) 108 Const. 35S prom. P2113 4354 Larger seed size; T1 and T2 seed larger than controls G154 MADS (2-57) 114 Const. 35S prom. P1223 4070 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G155 MADS (1-57) 116 Const. 35S prom. P13436 4626 Greater sens. to glucose (5%) G155 MADS (1-57) 116 Const. 35S prom. P13436 4626 Early flowering G155 MADS (1-57) 116 Const. 35S prom. P13436 4626 Greater sens. to mannitol (300 mM) G155 MADS (1-57) 116 Const. 35S prom. P13436 4626 Terminal flower G156 MADS (2-57) 118 Knockout not Altered seed color; applicable pale seeds indicate reduced pigment levels, which would correlate with this transcription factor being a regulator of flavonoid biosynthesis G156 MADS (2-57) 118 Knockout not Altered seed oil applicable composition, increased levels of 18:1 fatty acids correlate with G156 having a role as a regulator of lipid biosynthesis G156 MADS (2-57) 118 Const. 35S prom. P183 3858 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G156 MADS (2-57) 118 2 comp. including P3354 4501 Significantly greater P5326 (AP1 lycopene in tomato prom.) plants G157 MADS (2-57) 120 Const. 35S prom. P184 3859 Altered flowering time (modest level of overexpression triggers early flowering, whereas a larger increase delays flowering) G158 MADS (2-57) 122 Const. 35S prom. P1479 4158 Inc. in leaf rhamnose G159 MADS (7-61) 124 2 comp. including P4955 4593 Significantly greater P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G159 MADS (7-61) 124 2 comp. including P4955 4593 Significantly greater P5287 (LTP1 lycopene in tomato prom.) plants G161 MADS (6-62) 126 Const. 35S prom. P1219 4069 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G162 MADS (2-57) 128 Const. 35S prom. P1958 4290 More seed oil content G162 MADS (2-57) 128 Const. 35S prom. P1958 4290 Inc. seed protein content G168 MADS (1-57) 130 Const. 35S prom. P1313 4107 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G172 MADS (12-68) 132 Const. 35S prom. P2410 4377 Early flowering G173 MADS (1-57) 134 Const. 35S prom. P2116 4355 Late flowering G175 WRKY 136 Const. 35S prom. P1235 4077 More tol. to (178-234, hyperosmotic stress; 372-428) inc. tol. to 150 mM NaCl or 9.4% sucrose G175 WRKY 136 Const. 35S prom. P1235 4077 More tol. to drought* (178-234, 372-428) G180 WRKY 140 Const. 35S prom. P191 3860 Decreased seed oil (118-174) content G180 WRKY 140 Const. 35S prom. P191 3860 Early flowering (118-174) G181 WRKY (98-154) 142 Const. 35S prom. P1036 4034 Early flowering G183 WRKY 144 Const. 35S prom. P1033 4032 Early flowering (307-368) G183 WRKY 144 Const. 35S prom. P1033 4032 Altered light response (307-368) and/or shade tol.; reduced hypocotyl elongation, constitutive photomorphogenesis G183 WRKY 144 Const. 35S prom. P1033 4032 Altered C/N sensing: (307-368) greater tol. to low nitrogen conditions in C/N sensing assay G184 WRKY 146 Const. 35S prom. P968 4011 Early flowering (295-352) G184 WRKY 146 Const. 35S prom. P968 4011 Small plant (295-352) G185 WRKY 148 Const. 35S prom. P1038 4035 Higher leaf (113-172) glucosinolate M39481 level G185 WRKY 148 Const. 35S prom. P1038 4035 Early flowering (113-172) G186 WRKY 150 Const. 35S prom. P1459 4151 More res. to Erysiphe (312-369) G187 WRKY 152 2 comp. including P6407 4599 Significantly greater (172-228) P5318 (STM soluble solids (Brix) in prom.) tomato plants G188 WRKY 154 Knockout not Greater susceptibility (175-222) applicable to Fusarium G188 WRKY 154 Knockout not More tol. to (175-222) applicable hyperosmotic stress; inc. tol. to 150 mM NaCl, 300 mM mannitol, 9.4% sucrose or 5% glucose G188 WRKY 154 Const. 35S prom. P194 3861 More tol. to drought* (175-222) and better recovery from drought treatment* G189 WRKY 156 Const. 35S prom. P970 4013 Greater leaf size (240-297) G189 WRKY 156 Const. 35S prom. P970 4013 Altered C/N sensing: (240-297) greater tol. to low nitrogen conditions in C/N sensing assay G190 WRKY 158 2 comp. including P5142 4596 Significantly greater (110-169) P5318 (STM lycopene in tomato prom.) plants G192 WRKY 160 Const. 35S prom. P196 3862 Late flowering (128-185) G192 WRKY 160 Const. 35S prom. P196 3862 Decreased seed oil (128-185) content G196 WRKY 164 Const. 35S prom. P1232 4075 Greater tol. to NaCl (223-283) (determined with 150 mM NaCl) G198 MYB- 168 Const. 35S prom. P794 3971 Late flowering (R1)R2R3 (14-117) G200 MYB- 170 Knockout not Altered C/N sensing: (R1)R2R3 applicable accumulated more (12-116) anthocyanin in C/N sensing assay G200 MYB- 170 Const. 35S prom. P1349 4112 Altered C/N sensing: (R1)R2R3 greater tol. to low (12-116) nitrogen conditions in C/N sensing assay G200 MYB- 170 Const. 35S prom. P1349 4112 Altered leaves; small, (R1)R2R3 light green pointed (12-116) leaves G200 MYB- 170 Const. 35S prom. P1349 4112 Early flowering (R1)R2R3 (12-116) G201 MYB- 172 Const. 35S prom. P3 3793 Higher seed protein (R1)R2R3 content (14-114) G201 MYB- 172 Const. 35S prom. P3 3793 Decreased seed oil (R1)R2R3 content (14-114) G202 MYB- 174 Const. 35S prom. P4 3794 Decreased seed oil (R1)R2R3 content (13-116) G206 MYB- 176 Const. 35S prom. P818 3982 Large seeds (R1)R2R3 (13-116) G208 MYB- 180 Const. 35S prom. P781 3964 Early flowering (R1)R2R3 (14-116) G211 MYB- 182 Const. 35S prom. P1564 4190 Altered leaf (R1)R2R3 biochemistry; inc. leaf (24-137) xylose G211 MYB- 182 Const. 35S prom. P1564 4190 Reduced apical (R1)R2R3 dominance (24-137) G211 MYB- 182 Const. 35S prom. P1564 4190 Darker green (R1)R2R3 (24-137) G211 MYB- 182 Const. 35S prom. P1564 4190 Altered leaf shape; (R1)R2R3 rounded, serrated (24-137) leaves with short petioles G211 MYB- 182 2 comp. including P4359 4551 Increased lycopene in (R1)R2R3 P5287 (LTP1 tomato fruit when (24-137) prom.) expressed under LTP1 promoter G211 MYB- 182 2 comp. including P4359 4551 Increased lycopene in (R1)R2R3 P5318 (STM tomato fruit when (24-137) prom.) expressed under STM promoter G212 MYB- 184 Const. 35S prom. P819 3983 Partially to fully (R1)R2R3 glabrous on adaxial (15-116) surface of leaves G214 MYB- 186 Const. 35S prom. P10 3795 Late flowering related (25-71) G214 MYB- 186 Const. 35S prom. P10 3795 Inc. leaf fatty acids related (25-71) G214 MYB- 186 Const. 35S prom. P10 3795 Altered seed prenyl related (25-71) lipids; inc. seed lutein G214 MYB- 186 Const. 35S prom. P10 3795 Altered leaf prenyl related (25-71) lipids; inc. leaf chlorophyll and carotenoids G217 MYB- 188 Const. 35S prom. P798 3974 Inc. seed 20:2 fatty related (8-55) acid in T2 lines G222 MYB- 190 Const. 35S prom. P795 3972 Decreased seed oil (R1)R2R3 content (13-119) G222 MYB- 190 Const. 35S prom. P795 3972 Higher seed protein (R1)R2R3 content (13-119) G224 PMR (7-114) 192 Const. 35S prom. P2716 4454 Inc. tol. to cold (8 C.) G224 PMR (7-114) 192 Const. 35S prom. P2716 4454 Altered light response; greater shade tol.; lack of shade avoidance phenotype; long petioles G224 PMR (7-114) 192 Const. 35S prom. P2716 4454 Greater germination and seedling vigor on 5% glucose G229 MYB- 200 Const. 35S prom. P14 3796 Decreased seed protein (R1)R2R3 (14-120) G229 MYB- 200 Const. 35S prom. P14 3796 Higher seed oil content (R1)R2R3 (14-120) G229 MYB- 200 Const. 35S prom. P14 3796 Up-regulation of genes (R1)R2R3 involved in secondary (14-120) metabolism G231 MYB- 204 Const. 35S prom. P15 3797 Inc. leaf unsaturated (R1)R2R3 fatty acids (14-118) G231 MYB- 204 Const. 35S prom. P15 3797 More seed oil content (R1)R2R3 (14-118) G231 MYB- 204 Const. 35S prom. P15 3797 Altered seed protein; (R1)R2R3 decreased seed protein (14-118) content G233 MYB- 206 Const. 35S prom. P16 3798 Greater resistance to (R1)R2R3 Erysiphe, Sclerotinia (13-115) or Botrytis G233 MYB- 206 Const. 35S prom. P16 3798 Decreased tol. to 5% (R1)R2R3 glucose in a sugar (13-115) sensing assay G233 MYB- 206 Const. 35S prom. P16 3798 Decreased seed oil (R1)R2R3 content (13-115) G233 MYB- 206 Knockout not Decreased seed oil (R1)R2R3 applicable content (13-115) G234 MYB- 208 Const. 35S prom. P201 3864 Altered C/N sensing: (R1)R2R3 greater tol. to low (14-115) nitrogen conditions in C/N sensing assay G234 MYB- 208 Const. 35S prom. P201 3864 Altered light response; (R1)R2R3 greater shade tol.; lack (14-115) of shade avoidance phenotype G234 MYB- 208 Const. 35S prom. P201 3864 Late flowering (R1)R2R3 (14-115) G234 MYB- 208 Const. 35S prom. P201 3864 Smaller plant (R1)R2R3 (14-115) G237 MYB- 210 Const. 35S prom. P17 3799 Altered C/N sensing: (R1)R2R3 greater tol. to low (11-113) nitrogen conditions in C/N sensing assay G237 MYB- 210 Const. 35S prom. P17 3799 Higher leaf insoluble (R1)R2R3 sugars levels (11-113) G237 MYB- 210 Const. 35S prom. P17 3799 More res. to Erysiphe (R1)R2R3 (11-113) G237 MYB- 210 2 comp. including P4877 4590 Significantly greater (R1)R2R3 P5303 (PD prom.) lycopene in tomato (11-113) plants G237 MYB- 210 2 comp. including P4877 4590 Significantly greater (R1)R2R3 P5297 (PG prom.) lycopene in tomato (11-113) plants G241 MYB- 212 Knockout not Inc. seed protein (R1)R2R3 applicable content (14-114) G241 MYB- 212 Knockout not Decreased seed oil (R1)R2R3 applicable content (14-114) G241 MYB- 212 Const. 35S prom. P817 3981 Altered sugar sensing; (R1)R2R3 reduced hypocotyl (14-114) elongation and cotyledon expansion on 5% glucose G241 MYB- 212 Const. 35S prom. P817 3981 Microarrays on (R1)R2R3 overexpressing lines (14-114) showed activation of stress tolerance response pathways including components: CBF1, CBF2 and several genes indicative of osmotic stress tolerance G246 MYB- 216 Const. 35S prom. P13836 4648 Early flowering (R1)R2R3 (57-159) G246 MYB- 216 Const. 35S prom. P13836 4648 Altered light response; (R1)R2R3 greater shade tol.; lack (57-159) of shade avoidance phenotype; pale, elongated hypocotyls, long petioles, internode elongation between rosette leaves, leaves positioned in a vertical orientation G247 MYB- 218 Const. 35S prom. P1246 4081 Altered trichome (R1)R2R3 distribution (ectopic (15-116) formation of trichomes on the abaxial leaf surface); reduced trichome density G248 MYB- 220 Const. 35S prom. P994 4024 Inc. susceptibility to (R1)R2R3 Botrytis (264-332) G249 MYB- 222 Const. 35S prom. P204 3865 Late flowering (R1)R2R3 (19-116) G249 MYB- 222 Const. 35S prom. P204 3865 Delayed senescence (R1)R2R3 (19-116) G253 MYB- 224 Const. 35S prom. P15484 4711 Smaller plants (R1)R2R3 (16-116) G253 MYB- 224 Const. 35S prom. P15484 4711 Heart shaped and (R1)R2R3 darker green leaves (16-116) G253 MYB- 224 Const. 35S prom. P15484 4711 Short inflorescence (R1)R2R3 internodes (16-116) G254 MYB- 226 Const. 35S prom. P205 3866 Altered sugar sensing; related (60-106) decreased germination and growth on 5% glucose G256 MYB- 230 Const. 35S prom. P792 3970 Better germination and (R1)R2R3 growth in cold (8 C.) (14-116) G258 MYB- 232 Const. 35S prom. P1447 4147 Smaller plant (R1)R2R3 (24-124) G259 HS (40-131) 234 Const. 35S prom. P1397 4134 Inc. ?-tocopherol in seeds of T2 lines G261 HS (15-106) 236 Const. 35S prom. P206 3867 Increased susceptibility to Botrytis G261 HS (15-106) 236 Const. 35S prom. P206 3867 Altered light response; greater shade tol.; lack of shade avoidance phenotype; seedlings were slightly larger than controls under white light G263 HS (14-105) 238 Const. 35S prom. P207 3868 Decreased root growth on sucrose medium, root specific expression G264 HS (23-114) 240 Const. 35S prom. P1330 4109 Significant inc. in leaf glucosinolate M39481 G268 AKR (186-689) 242 Const. 35S prom. P15573 4726 Inc. biomass; inc. leaf size and vegetative biomass G270 AKR (259-424) 244 2 comp. including P4398 4560 Significantly greater P5319 (AS1 tomato plant volume prom.) G271 AKR (41-106, 246 Const. 35S prom. P209 3869 Altered light response; 325-363) greater shade tol.; lack of shade avoidance phenotype G274 AKR (94-600) 248 Const. 35S prom. P211 3870 Altered leaf insoluble sugars; inc. leaf arabinose G275 AKR (308-813) 250 Const. 35S prom. P1709 4234 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G278 AKR (2-593) 252 Const. 35S prom. P841 3988 Inc. susceptibility to Sclerotinia G280 AT-hook 254 Const. 35S prom. P1701 4230 Smaller plant (97-104, 130-137-155-162, 185-192) G280 AT-hook 254 Const. 35S prom. P1701 4230 Altered leaf prenyl (97-104, lipids; inc. delta and 130-137-155-162, gamma tocopherol 185-192) G287 MISC (293-354) 256 Const. 35S prom. P13371 4614 Inc. biomass; inc. rosette biomass at later stages of development G291 MISC (132-160) 258 Const. 35S prom. P219 3871 More seed oil content G303 HLH/MYC 260 Const. 35S prom. P1410 4140 Inc. tol. to (96-155) hyperosmotic stress; better germination on 9.4% sucrose or 150 mM NaCl G303 HLH/MYC 260 Const. 35S prom. P1410 4140 More tol. to drought* (96-155) and better recovery from drought treatment* G303 HLH/MYC 260 Const. 35S prom. P1410 4140 Altered light response; (96-155) greater shade tol.; lack of shade avoidance phenotype G307 SCR (292-357, 262 Const. 35S prom. P224 3872 Altered leaf insoluble 417-502, sugars; increases in 505-580) galactose, decreases in arabinose and mannose G308 SCR (239-304, 264 Const. 35S prom. P225 3873 Altered sugar sensing; 364-449, no germination on 5% 452-527) glucose G309 SCR (223-288, 266 Const. 35S prom. P13437 4627 Late flowering 342-427, 431-505) G309 SCR (223-288, 266 Const. 35S prom. P13437 4627 Smaller plants 342-427, 431-505) G309 SCR (223-288, 266 Const. 35S prom. P13437 4627 Darker green leaves 342-427, 431-505) G312 SCR (289-355, 268 Const. 35S prom. P1975 4298 Greater tol. to NaCl 414-503, (determined with 150 mM 503-583) NaCl) G314 SCR (113-180) 270 Const. 35S prom. P2714 4453 Inc. biomass; inc. leaf size in T2 plants G319 Z-CO-like 272 Const. 35S prom. P2508 4411 Greater biomass; (12-42) broader leaves G319 Z-CO-like 272 Const. 35S prom. P2508 4411 Late flowering (12-42) G319 Z-CO-like 272 Const. 35S prom. P2508 4411 Wrinkled, short broad (12-42) leaves G324 RING/C3H2C3 274 Const. 35S prom. P3299 4491 Late flowering (245-291) G324 RING/C3H2C3 274 Const. 35S prom. P3299 4491 Inc. biomass; inc. leaf (245-291) size and vegetative biomass G325 Z-CO-like 276 Const. 35S prom. P1497 4163 Inc. tol. to (5-28, 48-71) hyperosmotic stress; better germination on 9.4% sucrose or 150 mM NaCl G325 Z-CO-like 276 Const. 35S prom. P1497 4163 More tol. to drought* (5-28, 48-71) G326 Z-CO-like 278 Const. 35S prom. P229 3874 Altered C/N sensing: (11-94, 354-400) accumulated more anthocyanin in C/N sensing assay G328 Z-CO-like 280 2 comp. including P3955 4533 Significantly greater (12-78) P5326 (AP1 lycopene in tomato prom.) plants G328 Z-CO-like 280 2 comp. including P3955 4533 Significantly greater (12-78) P5297 (PG prom.) lycopene in tomato plants G343 GATA/Zn 282 Const. 35S prom. P22 3800 Greater resistance to (178-214) glyphosate G343 GATA/Zn 282 Const. 35S prom. P22 3800 Smaller plant (178-214) G344 GATA/Zn 284 Const. 35S prom. P1465 4153 More sensitive to (166-192) chilling in germination assay G344 GATA/Zn 284 Const. 35S prom. P1465 4153 Altered sugar sensing (166-192) phenotype: more sensitive to glucose in a germination assay G346 GATA/Zn 286 Const. 35S prom. P23 3801 Altered leaf fatty acids (196-221) G346 GATA/Zn 286 Const. 35S prom. P23 3801 Decreased seed oil (196-221) content G347 Z-LSDlike 288 Const. 35S prom. P1750 4249 Altered C/N sensing: (9-39, 50-70, greater tol. to low 80-127) nitrogen conditions in C/N sensing assay G347 Z-LSDlike 288 Const. 35S prom. P1750 4249 Decreased seed oil (9-39, 50-70, content 80-127) G351 Z-C2H2 290 Const. 35S prom. P3312 4495 Altered light response; (77-97, 118-140) greater shade tol.; lack of shade avoidance phenotype; leaves in a vertical orientation, light green coloration G353 Z-C2H2 292 Const. 35S prom. P1344 4110 More tol. to (41-61, 84-104) hyperosmotic stress; inc. seedling vigor on PEG G353 Z-C2H2 292 Const. 35S prom. P1344 4110 More tol. to drought* (41-61, 84-104) and better recovery from drought treatment* G353 Z-C2H2 292 Const. 35S prom. P1344 4110 Smaller plant (41-61, 84-104) G353 Z-C2H2 292 Const. 35S prom. P1344 4110 Flower; short pedicels, (41-61, 84-104) downward pointing siliques G353 Z-C2H2 292 Const. 35S prom. P1344 4110 Altered leaf (41-61, 84-104) development G354 Z-C2H2 294 Const. 35S prom. P1762 4251 Smaller plant (42-62, 88-109) G354 Z-C2H2 294 Const. 35S prom. P1762 4251 Altered light response (42-62, 88-109) and/or shade tol.; constitutive photomorphogenesis, abnormal cotyledons, elongated, thickened hypocotyls, short petioles G354 Z-C2H2 294 Const. 35S prom. P1762 4251 Flower; short pedicels, (42-62, 88-109) downward pointing siliques G354 Z-C2H2 294 Const. 35S prom. P1762 4251 More tol. to drought* (42-62, 88-109) and better recovery from drought treatment* G355 Z-C2H2 296 Const. 35S prom. P1763 4252 Enhanced growth (49-69, 94-116) under limiting phosphate in root growth assay G355 Z-C2H2 296 Const. 35S prom. P1763 4252 Greater tol. to NaCl (49-69, 94-116) (determined with 150 mM NaCl) G359 Z-C2H2 298 Const. 35S prom. P2379 4363 Altered light response; (49-69) greater shade tol.; lack of shade avoidance phenotype G361 Z-C2H2 300 Const. 35S prom. P25 3802 Late flowering (43-63) G362 Z-C2H2 302 Const. 35S prom. P1498 4164 Late flowering (62-82) G362 Z-C2H2 302 Const. 35S prom. P1498 4164 Smaller plant (62-82) G362 Z-C2H2 302 Const. 35S prom. P1498 4164 Ectopic trichome (62-82) formation; high trichome densities on sepals and ectopic trichomes on carpels G362 Z-C2H2 302 Const. 35S prom. P1498 4164 More pigmentation in (62-82) seed and embryos and in other organs G363 Z-C2H2 304 2 comp. including Significantly greater (87-108) P5287 (LTP1 lycopene in tomato prom.) plants G366 Z-C2H2 306 Const. 35S prom. P2654 4443 Lethal when (40-60) constitutively overexpressed G370 Z-C2H2 308 Knockout not Smaller plant (97-117) applicable G370 Z-C2H2 308 Knockout not Shiny leaves (97-117) applicable G370 Z-C2H2 308 Knockout not More sens. to PEG; (97-117) applicable reduced seedling vigor G370 Z-C2H2 308 Const. 35S prom. P2396 4373 Ectopic trichome (97-117) formation G371 RING/C3HC4 310 Const. 35S prom. P245 3875 Inc. susceptibility to (21-74) Botrytis G372 RING/C3HC4 312 Const. 35S prom. P15367 4699 Inc. leaf size; longer (141-180) leaves G372 RING/C3HC4 312 Const. 35S prom. P15367 4699 Late flowering (141-180) G374 Z-ZPF (35-67, 314 Knockout not Embryo lethal 286-318) applicable G375 Z-Dof (75-103) 316 Const. 35S prom. P1499 4165 Altered light response; greater shade tol.; lack of shade avoidance phenotype; vertically oriented leaves, elongated hypocotyls G377 RING/C3H2C3 318 Const. 35S prom. P1354 4116 Altered light response; (85-128) greater shade tol.; lack of shade avoidance phenotype G378 RING/C3H2C3 320 Const. 35S prom P247 3876 Greater res. to (196-237) Erysiphe G380 RING/C3H2C3 322 Const. 35S prom P15009 4654 Late flowering (637-677) G383 GATA/Zn 324 2 comp. including P4352 4549 Significantly greater (77-102) P5318 (STM lycopene in tomato prom.) plants G384 HB (14-77) 326 Const. 35S prom. P27 3803 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G385 HB (60-123) 328 Const. 35S prom. P248 3877 Smaller plants G385 HB (60-123) 328 Const. 35S prom. P248 3877 Short inflorescence stems G385 HB (60-123) 328 Const. 35S prom. P248 3877 Darker green plants G386 HB (133-193) 330 Const. 35S prom. P15647 4732 More anthocyanin production G388 HB (98-158) 332 Knockout not Altered light response; applicable greater shade tol.; lack of shade avoidance phenotype G390 HB (18-81) 334 Const. 35S prom. P829 3986 Altered shoot development G390 HB (18-81) 334 Const. 35S prom. P829 3986 Early flowering G391 HB (25-85) 336 Const. 35S prom. P249 3878 Altered shoot development G394 HB (121-182) 338 Const. 35S prom. P786 3967 More sensitive to growth in cold (8 C.) G409 HB (64-124) 340 Const. 35S prom. P825 3985 Greater resistance to Erysiphe G416 HB (451-511) 342 Const. 35S prom. P1984 4300 Early flowering G418 HB (500-560) 344 Const. 35S prom. P821 3984 Greater tol. to Pseudomonas G418 HB (500-560) 344 Const. 35S prom. P821 3984 Decreased seed protein content G419 HB (392-452) 346 Const. 35S prom. P784 3966 More tol. to potassium- free medium G427 HB (307-370) 348 Const. 35S prom. P1900 4279 More seed oil content G427 HB (307-370) 348 Const. 35S prom. P1900 4279 Decreased seed protein content G427 HB (307-370) 348 Const. 35S prom. P1900 4279 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G428 HB (229-292) 350 Const. 35S prom. P29 3804 Higher leaf insoluble sugars G428 HB (229-292) 350 Const. 35S prom. P29 3804 Altered leaf shape G431 HB (286-335) 352 Const. 35S prom. P783 3965 Sterile plants G434 HB (39-99) 354 Const. 35S prom. P3308 4493 Late flowering G435 HB (4-67) 356 Const. 35S prom. P30 3805 Higher leaf insoluble sugars G435 HB (4-67) 356 Const. 35S prom. P30 3805 Altered light response; greater shade tol.; lack of shade avoidance phenotype G435 HB (4-67) 356 2 comp. including P3771 4529 Significantly greater P5284 (RBCS3 lycopene in tomato prom.) plants G438 HB (22-85) 358 Knockout not Altered stem applicable morphology; reduced lignin G438 HB (22-85) 358 Knockout not Altered architecture; applicable reduced branching G438 HB (22-85) 358 Const. 35S prom. P1687 4222 Inc. biomass at late stage of development G438 HB (22-85) 358 Const. 35S prom. P1687 4222 Larger, flatter leaves at late stage of development G440 AP2 (122-184) 360 Const. 35S prom. P258 3879 Greater resistance to Erysiphe G442 AP2 (66-138) 362 Const. 35S prom. P909 3997 Altered light response; greater shade tol.; lack of shade avoidance phenotype G446 ARF (53-389) 364 Const. 35S prom. P2621 4434 Altered branching; secondary branch terminated in pin-like structure G446 ARF (53-389) 364 Const. 35S prom. P2621 4434 Altered flowers; large, abnormal fourth whorl organs that were long and thin, in some cases appeared to comprise only a single carpel, and were capped by an excessively large stigma G446 ARF (53-389) 364 Const. 35S prom. P2621 4434 Altered leaf shape; curled leaves G447 ARF (22-356) 366 Const. 35S prom. P1196 4063 Reduced size G447 ARF (22-356) 366 Const. 35S prom. P1196 4063 Altered cotyledon shape G447 ARF (22-356) 366 Const. 35S prom. P1196 4063 Darker green leaves G450 IAA (6-14, 368 Const. 35S prom. P1228 4074 Inc. seed size 78-89, 112-128, 180-217) G450 IAA (6-14, 368 2 comp. including P4012 4538 Significantly greater 78-89, P5318 (STM soluble solids (Brix) in 112-128, prom.) tomato plants 180-217) G450 IAA (6-14, 368 2 comp. including P4012 4538 Significantly greater 78-89, P5318 (STM lycopene in tomato 112-128, prom.) plants 180-217) G451 IAA (12-20, 370 Const. 35S prom. P9081 5103 Decreased seed protein 57-68, content 76-92, 131-164) G456 IAA (7-14, 372 Const. 35S prom. P39 3806 Decreased seed protein 71-81, content 120-153, 185-221) G456 IAA (7-14, 372 Const. 35S prom. P39 3806 Greater seed oil 71-81, content 120-153, 185-221) G464 IAA (20-28, 378 Const. 35S prom. P42, 3808, Better germination and 71-82, P1226 4072 growth in heat (32 C.) 126-142, 187-224) G464 IAA (20-28, 378 Const. 35S prom. P42, 3808, Greater seed oil 71-82, P1226 4072 content 126-142, 187-224) G464 IAA (20-28, 378 Const. 35S prom. P42, 3808, Narrow rolled leaves 71-82, P1226 4072 126-142, 187-224) G464 IAA (20-28, 378 Const. 35S prom. P42, 3808, Decreased seed protein 71-82, P1226 4072 content 126-142, 187-224) G468 IAA (86-102, 380 Const. 35S prom. P2466 4399 Altered light response; 141-171) greater shade tol.; lack of shade avoidance phenotype G468 IAA (86-102, 380 Const. 35S prom. P2466 4399 Wrinkled leaves 141-171) G470 ARF (61-393) 382 Const. 35S prom. P44 3809 Altered fertility; short stamen filaments G475 SBP (53-127) 384 Const. 35S prom. P45 3810 Early flowering G477 SBP (108-233) 386 Const. 35S prom. P268 3880 Inc. susceptibility to Sclerotinia G477 SBP (108-233) 386 Const. 35S prom. P268 3880 Greater sens. to oxidative stress G478 SBP (186-281) 388 Const. 35S prom. P2017 4313 Altered light response; greater shade tol.; lack of shade avoidance phenotype; long petioles G478 SBP (186-281) 388 Const. 35S prom. P2017 4313 Altered sugar sensing; more sens. to 5% glucose G502 NAC (10-155) 402 Knockout not More sens. to osmotic applicable stress; reduced germination and slower growth in 150 mM NaCl or 5% glucose G504 NAC (16-178) 404 Const. 35S prom. P1511 4172 Altered seed oil composition; decreased seed oil composition and content; increase in 18:2 fatty acid and decrease in 20:1 fatty acid G505 NAC (20-170) 406 Const. 35S prom. P273 3881 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G509 NAC (13-169) 408 Knockout not Greater total seed oil applicable and protein content G519 NAC (10-131) 416 Const. 35S prom. P281 3883 More seed oil content G521 NAC (7-156) 418 Const. 35S prom. P282 3884 Altered leaf cell expansion; very small, slow growing, leaves, indicating defect in cell elongation G522 NAC (10-165) 420 2 comp. including P4942 4592 Significantly greater P6506 (35S prom.) soluble solids (Brix) in tomato plants G522 NAC (10-165) 420 2 comp. including P4942 4592 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G525 NAC (23-167) 422 Const. 35S prom. P56 3817 Greater tol. to Pseudomonas G525 NAC (23-167) 422 Const. 35S prom. P56 3817 Inc. leaf insoluble sugars G526 NAC (21-149) 424 Const. 35S prom. P285 3885 Increased sens. to hyperosmotic stress (300 mM mannitol or PEG) G536 GF14 (226-233) 426 Const. 35S prom. P292 3886 Decreased germination and growth on 5% glucose G545 Z-C2H2 428 Const. 35S prom. P59 3818 Inc. sens. to 150 mM (82-102, NaCl 136-154) G545 Z-C2H2 428 Const. 35S prom. P59 3818 Greater susceptibility (82-102, to Erysiphe 136-154) G545 Z-C2H2 428 Const. 35S prom. P59 3818 Greater susceptibility (82-102, to Pseudomonas 136-154) G545 Z-C2H2 428 Const. 35S prom. P59 3818 Greater susceptibility (82-102, to Fusarium 136-154) G545 Z-C2H2 428 Const. 35S prom. P59 3818 Greater tol. to (82-102, phosphate-free 136-154) medium G545 Z-C2H2 428 Const. 35S prom. P59 3818 Altered C/N sensing: (82-102, accumulated more 136-154) anthocyanin in C/N sensing assay G546 RING/C3H2C3 430 Const. 35S prom. Decreased sens. to (114-155) ABA G549 MISC (1-395) 432 Const. 35S prom. P2581 4424 Altered inflorescence determinacy; at lower inflorescence nodes, shoot-like structures developed in place of single flowers, whereas higher up the inflorescence, flowers had reduced fertility and had organs with bract-like features G549 MISC (1-395) 432 Const. 35S prom. P2581 4424 Smaller plants G549 MISC (1-395) 432 Const. 35S prom. P2581 4424 Early flowering G550 Z-Dof (134-180) 434 Const. 35S prom. P1987 4301 Altered flowers: early flowers were small with poor organ formation, late flowers were normal G550 Z-Dof (134-180) 434 Const. 35S prom. P1987 4301 More sens. to heat (32 C.) in a growth assay G550 Z-Dof (134-180) 434 Const. 35S prom. P1987 4301 Higher anthocyanin level G551 HB (73-133) 436 2 comp. including P4709 5104 Significantly greater P5318 (STM soluble solids (Brix) in prom.) tomato plants G557 bZIP (90-150) 438 Const. 35S prom. P1249 4083 Darker green G557 bZIP (90-150) 438 Const. 35S prom. P1249 4083 Early flowering G557 bZIP (90-150) 438 Const. 35S prom. P1249 4083 Accelerated inflorescence maturation leading to earlier development of seed pods compared to controls G558 bZIP (45-105) 440 2 comp. including P3573 4513 Significantly greater P5319 (AS1 soluble solids (Brix) in prom.) tomato plants G558 bZIP (45-105) 440 2 comp. including P3573 4513 Significantly greater P5318 (STM soluble solids (Brix) in prom.) tomato plants G558 bZIP (45-105) 440 2 comp. including P3573 4513 Significantly greater P5319 (AS1 lycopene in tomato prom.) plants G558 bZIP (45-105) 440 2 comp. including P3573 4513 Significantly greater P5326 (AP1 tomato plant volume prom.) G559 bZIP (203-264) 442 Const. 35S prom. P295 3887 Loss of apical dominance G559 bZIP (203-264) 442 Const. 35S prom. P295 3887 Reduced fertility G561 bZIP (248-308) 444 Const. 35S prom. P1364 4120 More seed oil content G561 bZIP (248-308) 444 Const. 35S prom. P1364 4120 Greater tol. to potassium-free medium G561 bZIP (248-308) 444 Const. 35S prom. P1364 4120 Larger plants, more biomass G562 bZIP (253-315) 446 Const. 35S prom. P297 3888 Late flowering G565 bZIP (NA) 448 Const. 35S prom. P1365 4121 Early flowering G567 bZIP (210-270) 450 Const. 35S prom. P915 3999 Greater total seed oil/ protein content G567 bZIP (210-270) 450 Const. 35S prom. P915 3999 Greater total seed oil/ protein content G567 bZIP (210-270) 450 Const. 35S prom. P915 3999 Altered sugar sensing; decreased seedling vigor on 5% glucose G567 bZIP (210-270) 450 2 comp. including P4762 5105 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G568 bZIP (215-265) 452 Const. 35S prom. P1258 4086 Altered architecture; altered branching G571 bZIP (160-220, 454 Knockout not Delayed senescence 441-452) applicable G571 bZIP (160-220, 454 Knockout not Late flowering 441-452) applicable G571 bZIP (160-220, 454 Const. 35S prom. P1557 4186 Altered light response; 441-452) greater shade tol.; lack of shade avoidance phenotype G578 bZIP (36-96) 456 Const. 35S prom. P73 3819 Lethal when constitutively overexpressed G580 bZIP (162-218) 458 Const. 35S prom. P1556 4185 Altered flower development; flowers pointed downwards, petals were sometimes reduced in size, and siliques were short and curled G580 bZIP (162-218) 458 Const. 35S prom. P1556 4185 Altered inflorescences; inflorescence internodes were narrow causing the plant to appear short and bushy G580 bZIP (162-218) 458 2 comp. including P3657 4527 Significantly greater P5318 (STM soluble solids (Brix) in prom.) tomato plants G580 bZIP (162-218) 458 2 comp. including P3657 4527 Significantly greater P6506 (35S prom.) lycopene in tomato plants G580 bZIP (162-218) 458 2 comp. including P3657 4527 Significantly greater P5318 (STM lycopene in tomato prom.) plants G581 HLH/MYC 460 Const. 35S prom. P1329 4108 Altered C/N sensing: (330-387) greater tol. to low nitrogen conditions in C/N sensing assay G581 HLH/MYC 460 Const. 35S prom. P1329 4108 Late flowering (330-387) G581 HLH/MYC 460 Const. 35S prom. P1329 4108 Inc. seed size (330-387) G581 HLH/MYC 460 Const. 35S prom. P1329 4108 More tol. to low (330-387) nitrogen conditions; seedlings had less anthocyanin on low nitrogen G581 HLH/MYC 460 Const. 35S prom. P1329 4108 Altered seed color (330-387) G584 HLH/MYC 464 Const. 35S prom. P308 3889 Large seeds (409-466) G585 HLH/MYC 466 Const. 35S prom. P1489 4160 Reduced trichome (439-496) density G590 HLH/MYC 468 Knockout not Early flowering (194-251) applicable G590 HLH/MYC 468 Knockout not More seed oil content (194-251) applicable G590 HLH/MYC 468 Knockout not Altered C/N sensing: (194-251) applicable greater tol. to low nitrogen conditions in C/N sensing assay G591 HLH/MYC 470 Const. 35S prom. P77 3820 Greater res. to (149-206) Erysiphe G591 HLH/MYC 470 Const. 35S prom. P77 3820 Late flowering (149-206) G592 HLH/MYC 472 Const. 35S prom. P310 3890 Early flowering (282-340) G594 HLH/MYC 474 Const. 35S prom. P311 3891 Inc. susceptibility to (144-202) Sclerotinia G597 AT-hook 476 Const. 35S prom. P1417 4142 Altered seed protein (97-105, content 137-144) G598 DBP (205-263) 478 Const. 35S prom. P315 3892 Greater seed oil content G598 DBP (205-263) 478 Const. 35S prom. P315 3892 Altered leaf insoluble sugars; inc. galactose in leaf cell walls G600 DBP (115-290) 480 Const. 35S prom. P1214 4066 Altered leaves; small, flat, short and grayish or light green rosette leaves G600 DBP (115-290) 480 Const. 35S prom. P1214 4066 Early flowering G600 DBP (115-290) 480 Const. 35S prom. P1214 4066 Smaller plants G602 DBP (110-162) 482 Const. 35S prom. P79 3821 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G605 AT-hook 484 Const. 35S prom. P80 3822 Altered leaf fatty acid (72-80) composition; decreased 18:3, higher 16:0 fatty acids. Note: G605 is a paralog of G1944, SEQ ID NO: 1286, and thus it is expected that G605 overexpression may similarly be used to improve plant yield and quality G611 PCOMB 486 Const. 35S prom. P13387 4617 More tol. to drought* (655-874) G615 TEO (88-147) 488 Const. 35S prom. P1020 4028 Altered plant architecture; cotyledon fusion G615 TEO (88-147) 488 Const. 35S prom. P1020 4028 Little or no pollen production, poor filament elongation G616 TEO (39-95) 490 Const. 35S prom. P320 3893 Greater res. to Erysiphe G618 TEO (32-89) 492 Const. 35S prom. P1227 4073 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G622 ABI3/VP-1 496 Knockout Not Decreased seed 18:2 (294-375) applicable fatty acid content G624 ABI3/VP-1 498 Const. 35S prom. P2461 4398 Greater tol. to NaCl (327-406) (determined with 150 mM NaCl) G624 ABI3/VP-1 498 Const. 35S prom. P2461 4398 Greater biomass (327-406) G624 ABI3/VP-1 498 Const. 35S prom. P2461 4398 More tol. to drought* (327-406) and better recovery from drought treatment* G624 ABI3/VP-1 498 Const. 35S prom. P2461 4398 Greater tol. to low (327-406) phosphate G624 ABI3/VP-1 498 Const. 35S prom. P2461 4398 Late flowering (327-406) G627 MADS (1-57) 500 Const. 35S prom. P1030 4031 Early flowering G629 bZIP (92-152) 502 Const. 35S prom. P83 3823 Altered leaf morphology G629 bZIP (92-152) 502 Const. 35S prom. P83 3823 Higher seed protein content G630 bZIP (74-146) 504 Const. 35S prom. P84 3824 Higher seed protein content G635 TH (239-323) 508 Const. 35S prom. P1080 4047 Altered coloration (variegated) G635 TH (239-323) 508 Const. 35S prom. P1080 4047 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G635 TH (239-323) 508 2 comp. including P3619 4522 Significantly greater P5303 (PD prom.) soluble solids (Brix) in tomato plants G635 TH (239-323) 508 2 comp. including P3619 4522 Significantly greater P5303 (PD prom.) lycopene in tomato plants G638 TH (119-206) 512 Const. 35S prom. P325 3896 Altered flower development; reduced petal number and homeotic conversion G643 TH (47-85) 514 Const. 35S prom. P1093 4054 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G646 Z-Dof (55-97) 516 Const. 35S prom. P2513 4412 Altered leaves; very narrow downward curled darker green leaves G651 Z-C2H2 (5-31, 518 Const. 35S prom. P2812, 44,784,683 Altered leaf shape and 162-182, P15159 gray leaves 208-231) G651 Z-C2H2 (5-31, 518 Const. 35S prom. P2812, 44,784,683 Inc. sens. to cold (8 C.) 162-182, P15159 in a germination assay 208-231) G651 Z-C2H2 (5-31, 518 Const. 35S prom. P2812, 44,784,683 Altered root branching; 162-182, P15159 little or no secondary 208-231) root growth G651 Z-C2H2 (5-31, 518 Const. 35S prom. P2812, 44,784,683 Reduced plant size 162-182, P15159 208-231) G651 Z-C2H2 (5-31, 518 Const. 35S prom. P2812, 44,784,683 Altered flower 162-182, P15159 morphology 208-231) G652 Z-CLDSH 520 Knockout not Altered seed prenyl (28-49, 137-151, applicable lipids; more alpha- 182-196) tocopherol G652 Z-CLDSH 502 Knockout not Inc. leaf glucosinolate (28-49, 137-151, applicable M39480 182-196) G652 Z-CLDSH 520 Knockout not Altered light response; (28-49, 137-151, applicable greater shade tol.; lack 182-196) of shade avoidance phenotype G652 Z-CLDSH 520 Const. 35S prom. P2373 4361 Delayed senescence (28-49, 137-151, 182-196) G653 Z-LIM (10-61, 522 Const. 35S prom. P91 3825 Altered C/N sensing: 109-160) greater tol. to low nitrogen conditions in C/N sensing assay G657 MYB- 524 Const. 35S prom. P866 3990 Altered C/N sensing: (R1)R2R3 greater tol. to low (35-187) nitrogen conditions in C/N sensing assay G663 MYB- 526 Const. 35S prom. P97 3826 More anthocyanins in (R1)R2R3 leaf, root, seed (9-111) G663 MYB- 526 Const. 35S prom. P97 3826 Decreased seed oil (R1)R2R3 (9-111) G663 MYB- 526 Const. 35S prom. P97 3826 Higher seed protein (R1)R2R3 content (9-111) G666 MYB- 530 Const. 35S prom. P100 3828 Early flowering (R1)R2R3 (14-116) G666 MYB- 530 Const. 35S prom. P100 3828 Inc. res. to Erysiphe (R1)R2R3 (14-116) G668 MYB- 532 Const. 35S prom. P101 3829 Higher seed protein (R1)R2R3 content (14-115) G668 MYB- 532 Const. 35S prom. P101 3829 Decreased seed oil (R1)R2R3 content (14-115) G668 MYB- 532 Const. 35S prom. P101 3829 Reduced seed color (R1)R2R3 (14-115) G669 MYB- 534 Const. 35S prom. P102 3830 Altered leaf (R1)R2R3 morphology; rounded (15-118) leaves G670 MYB- 536 Const. 35S prom. P334 3897 Small plant (R1)R2R3 (14-122) G671 MYB- 538 Const. 35S prom. P995 4025 Altered inflorescence (R1)R2R3 stem structure; bolts (15-115) terminated in flowers or aborted flowers, secondary bolts replaced by leaf-like structures, bolts of small plants oddly shaped, changing direction slightly at each node G671 MYB- 538 Const. 35S prom. P995 4025 Reduced petal (R1)R2R3 abscission (15-115) G671 MYB- 538 Const. 35S prom. P995 4025 Altered leaf shape; true (R1)R2R3 leaves curled under, (15-115) petioles were upright, some plants had curled cotyledons G671 MYB- 538 Const. 35S prom. P995 4025 Small plant (R1)R2R3 (15-115) G671 MYB- 538 Const. 35S prom. P995 4025 Reduced fertility (R1)R2R3 (15-115) G674 MYB- 540 Const. 35S prom. P1613 4206 Darker green, (R1)R2R3 upwardly oriented (20-120) leaves G675 MYB- 542 2 comp. including P4019 4539 Significantly greater (R1)R2R3 P5319 (AS1 lycopene in tomato (13-116) prom.) plants G675 MYB- 542 2 comp. including P4019 4539 Significantly greater (R1)R2R3 P5284 (RBCS3 lycopene in tomato (13-116) prom.) plants G675 MYB- 542 2 comp. including P4019 4539 Significantly greater (R1)R2R3 P5318 (STM lycopene in tomato (13-116) prom.) plants G675 MYB- 542 2 comp. including P4019 4539 Significantly greater (R1)R2R3 P6506 (35S prom.) tomato plant volume (13-116) G675 MYB- 542 2 comp. including P4019 4539 Significantly greater (R1)R2R3 P5326 (AP1 tomato plant volume (13-116) prom.) G676 MYB- 544 Const. 35S prom. P105 3831 Reduced trichome (R1)R2R3 number, ectopic (17-119) trichome formation G680 MYB- 546 Const. 35S prom. P336 3898 Altered sugar sensing; related (25-71) reduced germination on 5% glucose G680 MYB- 546 Const. 35S prom. P336 3898 Late flowering related (25-71) G681 MYB- 548 Const. 35S prom. P1671 4218 Increase in leaf (R1)R2R3 glucosinolate M39480 (14-120) G707 HB (109-169) 552 Const. 35S prom. P15470 4707 Altered C/N sensing G707 HB (109-169) 552 Const. 35S prom. P15470 4707 Darker green leaves G707 HB (109-169) 552 Const. 35S prom. P15470 4707 Inc. pigment production G707 HB (109-169) 552 Const. 35S prom. P15470 4707 Late flowering G718 SBP (169-242) 558 Const. 35S prom. P341 3899 Higher seed protein content G718 SBP (169-242) 558 Const. 35S prom. P341 3899 Altered leaf fatty acid composition G718 SBP (169-242) 558 Const. 35S prom. P341 3899 Higher seed lutein content G718 SBP (169-242) 558 Const. 35S prom. P341 3899 Decreased seed oil content G720 GARP (301-349) 560 Const. 35S prom. P2071 4340 More freezing tolerant G720 GARP (301-349) 560 Knockout not More susceptible to applicable freezing G728 GARP (206-255) 562 Const. 35S prom. P1414 4141 Inc. tol. to cold (8 C.) G729 GARP (224-272) 564 2 comp. including P4528 4570 Significantly greater P5297 (PG prom.) tomato plant volume G729 GARP (224-272) 564 2 comp. including P4528 4570 Significantly greater P5324 (Cru prom.) tomato plant volume G730 GARP (169-217) 566 Const. 35S prom. P13422 4621 Reduced secondary root growth G730 GARP (169-217) 566 Const. 35S prom. P13422 4621 Abaxialization of adaxial surfaces G732 bZIP (31-91) 568 Const. 35S prom. P120 3834 One OE line had higher, another lower, seed protein content G732 bZIP (31-91) 568 Const. 35S prom. P120 3834 One OE line had more, another less seed oil content G732 bZIP (31-91) 568 Const. 35S prom. P120 3834 Reduced apical dominance G732 bZIP (31-91) 568 Const. 35S prom. P120 3834 Abnormal flowers G736 Z-Dof (54-111) 572 Const. 35S prom. P344 3900 Late flowering G736 Z-Dof (54-111) 572 Const. 35S prom. P344 3900 Altered leaf shape; small, rounded leaves with long petioles G738 Z-Dof (351-393) 574 Const. 35S prom. P1774 4257 Late flowering G738 Z-Dof (351-393) 574 Const. 35S prom. P1774 4257 Higher anthocyanin levels in leaf petioles G738 Z-Dof (351-393) 574 Const. 35S prom. P1774 4257 Smaller plant G740 Z-CLDSH 576 Const. 35S prom. P345 3901 Slow growth (24-42, 232-268) G744 RING/C3H2C3 578 Const. 35S prom. P15010 4655 Late flowering (176-217) G748 Z-Dof (112-140) 580 Const. 35S prom. P346 3902 Altered seed prenyl lipids; more lutein content G748 Z-Dof (112-140) 580 Const. 35S prom. P346 3902 Altered stem morphology; more vascular bundles in stem G748 Z-Dof (112-140) 580 Const. 35S prom. P346 3902 Late flowering G752 RING/C3H2C3 582 Const. 35S prom. P15012 4656 Late flowering (439-479) G760 NAC (12-156) 584 Const. 35S prom. P1359 4118 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G760 NAC (12-156) 584 Const. 35S prom. P1359 4118 Hypersensitive to ACC G760 NAC (12-156) 584 Const. 35S prom. P1359 4118 Reduced size G772 NAC (27-176) 586 Const. 35S prom. P868 3991 Altered light response; greater shade tol.; lack of shade avoidance phenotype G773 NAC (17-159) 588 Const. 35S prom. P352 3903 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G776 NAC (27-175) 590 Const. 35S prom. P354 3904 Altered seed fatty acid composition; decreased levels of seed 20:1 and 22:1 fatty acids G777 HLH/MYC 592 Const. 35S prom. P356 3905 Decreased seed oil (41-99) G777 HLH/MYC 592 Const. 35S prom. P356 3905 Greater leaf rhamnose (41-99) level G778 HLH/MYC 594 Const. 35S prom. P357 3906 Higher seed 18:1 fatty (210-267) acid content G779 HLH/MYC 596 Const. 35S prom. P1192 4061 Reduced fertility (117-174) G779 HLH/MYC 596 Const. 35S prom. P1192 4061 Altered flower; (117-174) homeotic transformations G782 HLH/MYC 598 Const. 35S prom. P128 3836 Altered sugar sensing; (2-60) greater tol. to sucrose (determined in 9.4% sucrose) G783 HLH/MYC 600 Const. 35S prom. P129 3837 Altered sugar sensing; (24-82) greater tol. to sucrose (determined in 9.4% sucrose) G789 HLH/MYC 602 Const. 35S prom. P1650 4215 Early flowering (253-310) G789 HLH/MYC 602 Const. 35S prom. P1650 4215 More susceptible to (253-310) Sclerotinia G789 HLH/MYC 602 Const. 35S prom. P1650 4215 More sens. to oxidative (253-310) stress (glyphosate or rose bengal) G791 HLH/MYC 604 Const. 35S prom. P363 3908 Decrease in 18:1 seed (68-127) fatty acid G791 HLH/MYC 604 Const. 35S prom. P363 3908 Altered leaf cell wall (68-127) polysaccharide composition G791 HLH/MYC 604 Const. 35S prom. P363 3908 Decrease in 18:2 leaf (68-127) fatty acids G798 Z-Dof (19-47) 608 Const. 35S prom. P132 3839 Altered light response; greater shade tol.; lack of shade avoidance phenotype G798 Z-Dof (19-47) 608 Const. 35S prom. P132 3839 Altered leaf shape; wavy leaves at early stages of growth G801 PCF (32-93) 610 Const. 35S prom. P366 3909 Greater tol. to NaCl (determined with 150 mM NaCl) G802 PCF (60-140) 612 Const. 35S prom. P367 3910 Altered inflorescence stem morphology; presence of lignified cells outside the phloem bundles was observed in one of the overexpressing lines G805 PCF (51-114) 614 Const. 35S prom. P370 3911 Increased susceptibility to Sclerotinia G807 HS (27-118) 616 Const. 35S prom. P1654 4216 Inc. seedling vigor and growth rate in T1 and T2 plants; seedlings were reproducibly larger, grew faster and showed longer hypocotyl and petioles G807 HS (27-118) 616 Const. 35S prom. P1654 4216 Altered light response; greater shade tol.; lack of shade avoidance phenotype; longer hypocotyl and petioles G807 HS (27-118) 616 Const. 35S prom. P1654 4216 Greater tol. to cold (8 C.); seedlings were larger and greener with almost no anthocyanin G811 HS (17-108) 618 Const. 35S prom. P15160 4684 Darker green leaves G811 HS (17-108) 618 Const. 35S prom. P15160 4684 Smaller plants G812 HS (29-120) 620 2 comp. including P3650 4525 Significantly greater P5324 (Cru prom.) tomato plant volume G812 HS (29-120) 620 2 comp. including P3650 4525 Significantly greater P5303 (PD prom.) tomato plant volume G818 HS (71-162) 622 Const. 35S prom. P1786 4259 Altered light response; greater shade tol.; lack of shade avoidance phenotype G831 AKR (96-612) 624 Const. 35S prom. P927 4000 Smaller plant G837 AKR (250-754) 626 Const. 35S prom. P873 3992 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G839 AKR (60-185, 628 Const. 35S prom. P1791 4262 Greater tol. to 290-353) nitrogen-limited medium; seedlings were larger, greener and had more root growth on nitrogen- limited media G839 AKR (60-185, 628 Const. 35S prom. P1791 4262 Late flowering 290-353) G843 MISC (60-119, 630 2 comp. including P4559 4574 Significantly greater 270-350) P6506 (35S prom.) lycopene in tomato plants G843 MISC (60-119, 630 2 comp. including P4559 4574 Significantly greater 270-350) P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G843 MISC (60-119, 630 2 comp. including P4559 4574 Significantly greater 270-350) P5326 (AP1 lycopene in tomato prom.) plants G843 MISC (60-119, 630 2 comp. including P4559 4574 Significantly greater 270-350) P5284 (RBCS3 soluble solids (Brix) in prom.) tomato plants G846 SWI/SNF 632 Const. 35S prom. P15686 4736 Gamete lethal; flowers (222-531, macroscopically 679-719, normal but poor 840-923) fertility and very few siliques G849 BPF-1 (324-403, 634 Knockout not Greater seed oil 505-591) applicable content; inc. total seed protein and oil content G852 SCR (299-364, 636 Const. 35S prom. P2720 4455 Larger biomass; long 427-515, broad leaves, 519-592) substantially greater biomass G852 SCR (299-364, 636 Const. 35S prom. P2720 4455 Late flowering 427-515, 519-592) G859 MADS (1-57) 638 Const. 35S prom. P1688 4223 Late flowering G861 MADS (2-57) 642 Const. 35S prom. P379 3912 Increase in 16:1 seed fatty acids G864 AP2 (119-181) 644 Const. 35S prom. P380 3913 Better germination in heat (32 C.) G864 AP2 (119-181) 644 Const. 35S prom. P380 3913 Greater tol. to drought* G864 AP2 (119-181) 644 Const. 35S prom. P380 3913 More sens. to growth in cold (8 C.) G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Inc. susceptibility to Erysiphe G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Higher seed protein content G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Reduced seed oil G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Inc. susceptibility to Botrytis G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Early flowering G865 AP2 (36-103) 646 Const. 35S prom. P381 3914 Altered morphology; numerous secondary inflorescence meristems G866 WRKY (43-300) 648 Const. 35S prom. P382 3915 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Altered seed fatty acid composition; higher seed 18:1 and 18:2 fatty acids levels G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Higher leaf fucose content G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Greater res. to Erysiphe or Botrytis G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Small and spindly plant G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Abnormal anther development G869 AP2 (110-165) 650 Const. 35S prom. P384 3917 Altered leaf fatty acids; lower 16:0 levels and higher 16:3 levels G872 AP2 (18-84) 652 Const. 35S prom. P385 3918 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G877 WRKY 654 Knockout not Embroyo lethal (272-328, applicable phenotype: potenital 487-603) herbicide target G878 WRKY 656 Const. 35S prom. P1345 4111 Delayed senescence (250-305, 415-475) G878 WRKY 656 Const. 35S prom. P1345 4111 Late flowering (250-305, 415-475) G878 WRKY 656 Const. 35S prom. P1345 4111 Darker green (250-305, 415-475) G878 WRKY 656 Const. 35S prom. P1345 4111 Shorter stems (250-305, 415-475) G881 WRKY 658 Const. 35S prom. P388 3919 Greater susceptibility (176-233) to Erysiphe G881 WRKY 658 Const. 35S prom. P388 3919 Greater susceptibility (176-233) to Botrytis G881 WRKY 658 2 comp. including P5557 4597 Significantly increased (176-233) P5318 (STM lycopene in tomato prom.) plants G883 WRKY 660 Const. 35S prom. P389 3920 Decreased seed lutein (245-302) G8884 WRKY 662 Const. 35S prom. P1351 4114 Greater tol. to NaCl (227-285, (determined with 150 mM 407-465) NaCl) G884 WRKY 662 Const. 35S prom. P1351 4114 Reduced size (227-285, 407-465) G892 RING/C3H2 664 Knockout not Altered seed protein C3 (177-270) applicable content G892 RING/C3H2 664 Knockout not Altered seed oil C3 (177-270) applicable content G896 Z-LSDlike 666 Knockout not Greater susceptibility (18-39) applicable to Fusarium G898 RING/C3H 668 Const. 35S prom. P1540 4181 Early flowering C4 (148-185) G903 Z-C2H2 670 Const. 35S prom. P138 3840 Altered leaf (68-92) morphology; narrow, twisted leaves G904 RING/C3H2 672 Const. 35S prom. P2055 4333 Altered C/N sensing: C3 (117-158) greater tol. to low nitrogen conditions in C/N sensing assay G905 RING/C3H2C3 674 Const. 35S prom. P15014 4657 Late flowering (118-159) G905 RING/C3H2C3 674 Const. 35S prom. P15014 4657 Altered leaf shape; (118-159) narrow, curled leaves G905 RING/C3H2C3 674 Const. 35S prom. P15014 4657 Altered sugar sensing; (118-159) inc. seedling vigor on 5% glucose G910 Z-CO-like 676 Const. 35S prom. P1770 4255 Late flowering (14-37, 77-103) G911 RING/C3H2C3 678 Const. 35S prom. P141 3841 Better growth on (86-129) potassium-free medium G911 RING/C3H2C3 678 Const. 35S prom. P141 3841 Higher seed protein (86-129) content G911 RING/C3H2C3 678 Const. 35S prom. P141 3841 Decreased seed oil (86-129) content G912 AP2 (51-118) 680 Const. 35S prom. P393 3921 More tolerant to freezing G912 AP2 (51-118) 680 Const. 35S prom. P393 3921 More tol. to drought* and better recovery from drought treatment* G912 AP2 (51-118) 680 Const. 35S prom. P393 3921 Altered pigment; darker green color G912 AP2 (51-118) 680 Const. 35S prom. P393 3921 Altered sugar sensing; reduced cotyledon expansion in 5% glucose G912 AP2 (51-118) 680 Const. 35S prom. P393 3921 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G916 WRKY 684 Const. 35S prom. P1233 4076 More tol. to drought* (293-349) and better recovery from drought treatment* G916 WRKY 684 Const. 35S prom. P1233 4076 Altered sugar sensing; (293-349) greater tol. to sucrose (determined in 9.4% sucrose) G916 WRKY 684 Const. 35S prom. P1233 4076 Altered C/N sensing: (293-349) greater tol. to low nitrogen conditions in C/N sensing assay G916 WRKY 684 Const. 35S prom. P1233 4076 Altered light response; (293-349) greater shade tol.; lack of shade avoidance phenotype; pale plants, disproportionately long hypocotyls and narrow cotyledons G917 MADS (2-57) 686 Const. 35S prom. P1637 4214 Darker green G917 MADS (2-57) 686 Const. 35S prom. P1637 4214 Leaves slightly flatter and more rounded, shorter petioles G921 WRKY 688 Const. 35S prom. P396 3922 Inc. sens. to (146-203) hyperosmotic stress (150 mM NaCl or PEG) G921 WRKY 688 Const. 35S prom. P396 3922 Serrated leaves (146-203) G932 MYB- 702 Const. 35S prom. P400 3925 Altered C/N sensing: (R1)R2R3 greater tol. to low (14-118) nitrogen conditions in C/N sensing assay G932 MYB- 702 Const. 35S prom. P400 3925 Darker green color (R1)R2R3 (14-118) G932 MYB- 702 Const. 35S prom. P400 3925 Smaller plants (R1)R2R3 (14-118) G937 GARP (197-246) 704 Const. 35S prom. P1744 4248 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G937 GARP (197-246) 704 2 comp. including P4527 4569 Significantly greater P5297 (PG prom.) tomato plant volume G938 EIL (96-104) 706 Const. 35S prom. P401 3926 Higher seed 16:0, 18:0, 20:0, and 18:3 fatty acid levels, lower seed 18:2, 20:1, 22:1 fatty acid levels G939 EIL (97-106) 708 Const. 35S prom. P402 3927 Dwarfed plants with compact inflorescences G956 NAC (NA) 710 Const. 35S prom. Late flowering G957 NAC (12-182) 712 Const. 35S prom. P1727 4243 Altered leaf shape; wrinkled, curled leaves G958 NAC (7-156) 714 Const. 35S prom. P1517 4173 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G961 NAC (12-180) 716 Knockout not More seed oil content applicable G961 NAC (12-180) 716 Const. 35S prom P13824 4647 Altered seed development and germination; seeds frequently aborted; seeds that were obtained were dark with white patches were visible on the seed coat G962 NAC (53-175) 718 Const. 35S prom P1852 4272 Inc. 16:0 leaf fatty acids, decreased 18:3 leaf fatty acids in T2 lines G963 NAC (NA) 720 Const. 35S prom P1520 4174 Late flowering G964 HB (126-186) 722 Knockout not Altered C/N sensing: applicable greater tol. to low nitrogen conditions in C/N sensing assay G964 HB (126-186) 722 Const. 35S prom P144 3843 More tolerant to heat (32 C.) in germination assay G965 HB (423-486) 724 Const. 35S prom. P405 3928 Increase in seed 18:1 fatty acid G971 AP2 (120-186) 726 Const. 35S prom. P1247 4082 Late flowering G971 AP2 (120-186) 726 Const. 35S prom. P1247 4082 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G971 AP2 (120-186) 726 Const. 35S prom. P1247 4082 Altered light response; greater shade tol.; lack of shade avoidance phenotype G977 AP2 (5-72) 734 Const. 35S prom. P1266 4089 Small plants G977 AP2 (5-72) 734 Const. 35S prom. P1266 4089 Darker green plants G977 AP2 (5-72) 734 Const. 35S prom. P1266 4089 Darker green wrinkled or curled leaves G977 AP2 (5-72) 734 Const. 35S prom. P1266 4089 Reduced fertility G979 AP2 (63-139, 736 Knockout not Altered seed 165-233) applicable development, ripening, and germination, developed into small, poorly fertile plants G979 AP2 (63-139, 736 Const. 35S prom. P1350 4113 Altered C/N sensing: 165-233) greater tol. to low nitrogen conditions in C/N sensing assay G987 SCR (395-462, 738 Knockout not Altered leaf fatty acids; 525-613, applicable reduction in 16:3 fatty 1027-1102, acids 1162-1255) G987 SCR (395-462, 738 Knockout not Altered leaf prenyl 525-613, applicable lipids: chlorophyll, 1027-1102, tocopherol, carotenoid, 1162-1255) presence of two xanthophylls, tocopherol not normally found in leaves; reduced chlorophyll a and b G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Inc. seed protein 277-366, content 371-444) G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Decreased seed oil 277-366, content 371-444) G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Enlarged floral organs, 277-366, short pedicels 371-444) G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Altered architecture; 277-366, reduced lateral 371-444) branching G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Altered stem 277-366, morphology; thicker 371-444) stem, altered distribution of vascular bundles, irregular development of vascular bundles G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Altered light response; 277-366, greater shade tol.; lack 371-444) of shade avoidance phenotype G988 SCR (150-217, 740 Const. 35S prom. P1475 4155 Altered C/N sensing: 277-366, accumulated more 371-444) anthocyanin in C/N sensing assay G989 SCR (121-186, 742 Const. 35S prom. P1768 4254 Altered C/N sensing: 238-326, greater tol. to low 327-399) nitrogen conditions in C/N sensing assay G989 SCR (121-186, 742 2 comp. including P4539 4572 Significantly greater 238-326, P5324 (Cru prom.) tomato plant volume 327-399) G989 SCR (121-186, 742 2 comp. including P4539 4572 Significantly greater 238-326, P5318 (STM plant volume in tomato 327-399) prom.) plants G991 IAA (7-14, 744 Const. 35S prom. P836 3987 Slightly smaller plants 48-59, 82-115, 128-164) G994 MYB- 748 Const. 35S prom. P145 3844 Late flowering (R1)R2R3 (14-123) G994 MYB- 748 Const. 35S prom. P145 3844 Smaller plants (R1)R2R3 (14-123) G996 MYB- 750 Const. 35S prom. P146 3845 Altered sugar sensing; (R1)R2R3 reduced germination (14-114) on 5% glucose G1007 AP2 (23-90) 754 2 comp. including P4002 4537 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G1007 AP2 (23-90) 754 2 comp. including P4002 4537 Significantly greater P5326 (AP1 lycopene in tomato prom.) plants G1007 AP2 (23-90) 754 2 comp. including P4002 4537 Significantly greater P5324 (Cru prom.) soluble solids (Brix) in tomato plants G1011 MADS (2-57) 756 Const. 35S prom. P1712 4235 Floral organ abscission was delayed, with stamens, petals, and sepals persisting following pollination G1011 MADS (2-57) 756 Const. 35S prom. P1712 4235 Altered trichomes; greater trichome density on sepals and ectopic trichomes on carpels G1011 MADS (2-57) 756 Const. 35S prom. P1712 4235 Altered leaf shape; rounded leaves G1011 MADS (2-57) 756 Const. 35S prom. P1712 4235 Early flowering G1012 WRKY (30-86) 758 Const. 35S prom. P1505 4167 Decreased leaf rhamnose G1013 WRKY 760 Const. 35S prom. P2416 4380 Slow growth rate (114-170) G1013 WRKY 760 Const. 35S prom. P2416 4380 Altered flower (114-170) development; sporadic defects in flower development G1013 WRKY 760 Const. 35S prom. P2416 4380 Altered light response; (114-170) greater shade tol.; lack of shade avoidance phenotype: upright leaf orientation, upright cotyledons G1013 WRKY 760 Const. 35S prom. P2416 4380 Altered leaf shape; (114-170) narrow downward curled leaves G1013 WRKY 760 Const. 35S prom. P2416 4380 Altered C/N sensing: (114-170) greater tol. to low nitrogen conditions in C/N sensing assay G1017 ARF (9-382) 762 Const. 35S prom. P15458 4706 Greater tol. to NaCl (determined with 150 mM NaCl) G1020 AP2 (28-95) 764 Const. 35S prom. P424 3932 Very small T1 plants G1023 AP2 (128-196) 766 Const. 35S prom. P426 3933 Smaller plants G1033 HMG (49-121) 768 Const. 35S prom. P13786 4638 Premature leaf senescence G1033 HMG (49-121) 768 Const. 35S prom. P13786 4638 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G1037 GARP (11-134, 770 Knockout not Early flowering 200-248) applicable G1037 GARP (11-134, 770 Const. 35S prom. P15001 4652 Greater tol. to NaCl 200-248) (determined with 150 mM NaCl) G1038 GARP (198-247) 772 Const. 35S prom. P148 3846 Altered leaf shape; rounded darker green leaves G1038 GARP (198-247) 772 Const. 35S prom. P148 3846 Decreased insoluble sugars G1040 GARP (109-158) 774 Const. 35S prom. P432 3934 Smaller and more rounded seeds G1043 WRKY 776 Const. 35S prom. P1271 4093 Inc. res. to Erysiphe (120-179) G1043 WRKY 776 Const. 35S prom. P1271 4093 Dark green glossy (120-179) leaves G1047 bZIP (129-180) 778 Const. 35S prom. P979 4015 Greater resistance to Fusarium G1048 bZIP (138-190) 780 Const. 35S prom. P149, 3847, Altered light response; P1257 4085 greater shade tol.; lack of shade avoidance phenotype G1048 bZIP (138-190) 780 Const. 35S prom. P149, 3847, Greater resistance to P1257 4085 Erysiphe orontii G1048 bZIP (138-190) 780 Const. 35S prom. P149, 3847, Greater seed protein P1257 4085 content G1049 bZIP (77-132) 782 Const. 35S prom. P1092 4053 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1050 bZIP (372-425) 784 Const. 35S prom. P1369 4122 Delayed senescence G1051 bZIP (189-250) 786 Const. 35S prom. P1084 4049 Late flowering G1052 bZIP (201-261) 788 Const. 35S prom. P1370 4123 Late flowering G1052 bZIP (201-261) 788 Const. 35S prom. P1370 4123 Altered seed prenyl lipids; decreased lutein and inc. xanthophyll 1 G1053 bZIP (74-120) 790 Const. 35S prom. P934 4002 Smaller plants G1053 bZIP (74-120) 790 2 comp. including P3599 4517 Significantly greater P5324 (Cru prom.) soluble solids (Brix) in tomato plants G1053 bZIP (74-120) 790 2 comp. including P359 3907 Significantly greater P5326 (AP1 tomato plant volume prom.) G1053 bZIP (74-120) 790 2 comp. including P359 3907 Significantly greater P5319 (AS1 tomato plant volume prom.) G1062 HLH/MYC 792 Knockout not Altered seed shape; (300-357) applicable twisted and wrinkled G1062 HLH/MYC 792 Knockout not Altered light response; (300-357) applicable constitutive photomorphogenesis, twisted hypocotyl G1062 HLH/MYC 792 Knockout not Altered light response; (300-357) applicable greater shade tol.; lack of shade avoidance phenotype G1062 HLH/MYC 792 Knockout not Altered response to (300-357) applicable ethylene; more severely stunted G1062 HLH/MYC 792 Knockout not Slow growth (300-357) applicable G1063 HLH/MYC 794 Const. 35S prom. P1702 4231 Altered leaf shape, (125-182) darker green color G1063 HLH/MYC 794 Const. 35S prom. P1702 4231 Altered inflorescence (125-182) development G1063 HLH/MYC 794 Const. 35S prom. P1702 4231 Altered flower (125-182) development, ectopic carpel tissue G1064 PCF (116-179) 796 Const. 35S prom. P1703 4232 Greater sens. to Botrytis G1068 AT-hook 800 Const. 35S prom. P444 3935 Altered sugar sensing; (143-150) reduced cotyledon expansion in 5% glucose G1076 AT-hook 806 Const. 35S prom. P452 3938 Lethal when (82-90, 90-233) constitutively overexpressed G1078 BZIPT2 (1-53, 808 2 comp. including P3580 4514 Significantly greater 440-550) P5284 (RBCS3 lycopene in tomato prom.) plants G1079 BZIPT2 (1-50) 810 Const. 35S prom. P453 3939 Late flowering G1082 BZIPT2 (1-53, 812 Const. 35S prom. P1083 4048 Altered light response; 503-613) long hypocotyls G1084 BZIPT2 (1-53, 814 Const. 35S prom. P980 4016 Inc. susceptibility to 490-619) Botrytis G1089 BZIPT2 816 Knockout not Altered sugar sensing; (425-500) applicable greater tol. to sucrose (determined in 9.4% sucrose) G1089 BZIPT2 816 Const. 35S. prom. P1423 4144 Developmental defects (425-500) at seedling stage G1090 AP2 (17-84) 818 Const. 35S prom. P458 3940 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1095 RING/C3H2C3 820 Const. 35S prom. P1355 4117 Inc. sensitivity to (134-159) ACC; seedlings germinated in the dark on ACC-containing media were more severely stunted than controls G1100 RING/C3H2C3 822 Const. 35S prom. P1353 4115 Altered light response; (96-137) greater shade tol.; lack of shade avoidance phenotype G1100 RING/C3H2C3 822 Const. 35S prom. P1353 4115 Large darker green (96-137) rosettes at late stage of development G1100 RING/C3H2C3 822 Const. 35S prom. P1353 4115 Stunted inflorescence (96-137) growth and abnormal flowers G1100 RING/C3H2C3 822 Const. 35S prom. P1353 4115 Slower growth rate (96-137) G1108 RING/C3H2C3 824 Const. 35S prom. P15018 4658 Altered sugar sensing; (363-403) inc. tol. to 5% glucose G1113 RING/C3H2C3 826 Const. 35S prom. P15019 4659 Inc. biomass; flat broad (85-128) leaves, inc. vegetative biomass G1113 RING/C3H2C3 826 Const. 35S prom. P15019 4659 Late flowering (85-128) G1128 AT-hook 828 Const. 35S prom. P1704 4233 Altered leaves; darker (78-86) green, narrow contorted leaves G1128 AT-hook 828 Const. 35S prom. P1704 4233 Altered senescence; (78-86) premature leaf and flower senescence G1128 AT hook 828 Const. 35S prom. P1704 4233 Reduced fertility; little (78-86) or no seed development G1129 HLH/MYC 830 Const. 35S prom. P1298 4101 Altered light response; (175-233) greater shade tol.; lack of shade avoidance phenotype G1133 HLH/MYC 832 Const. 35S prom. P466 3941 Decreased leaf lutein (260-317) G1136 HLH/MYC 836 Const. 35S prom. P3298 4490 Late flowering (408-465) G1136 HLH/MYC 836 Const. 35S prom. P3298 4490 Increased sens. to low (408-465) nitrogen G1137 HLH/MYC 838 Const. 35S prom. P938 4003 Altered light response; (257-314) greater shade tol.; lack of shade avoidance phenotype G1140 MADS (2-57) 840 Const. 35S prom. P939 4004 Altered flower development G1142 HLH/MYC 842 Const. 35S prom. P1989 4302 Late flowering (63-120) G1142 HLH/MYC 842 Const. 35S prom. P1989 4302 Altered leaf shape; (63-120) narrow leaves G1143 HLH/MYC 844 Const. 35S prom. P1301 4102 Decreased seed oil (25-82) content G1145 bZIP (227-270) 846 Const. 35S prom. P1263 4087 Reduced seed size G1145 bZIP (227-270) 846 Const. 35S prom. P1263 4087 Small, wrinkled seed shape G1146 PAZ (886-896) 848 Const. 35S prom. P1372 4124 Altered leaf development G1150 PAZ (887-907) 850 Const. 35S prom. P15631 4730 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1150 PAZ (887-907) 850 Const. 35S prom. P15631 4730 Late flowering G1150 PAZ (887-907) 850 Const. 35S prom. P15631 4730 Inc. biomass; T2 plants had substantial inc. in rosette size, considerably more vegetative biomass G1181 HS (23-114) 852 Const. 35S prom. P471 3943 Small T1 plants G1190 AKR (85-593) 854 Const. 35S prom. P1891 4276 More seed oil content G1196 AKR (179-254) 856 Knockout not Greater susceptibility applicable to Botrytis G1198 bZIP (173-223) 858 Const. 35S prom. P941 4005 More seed oil content G1198 bZIP (173-223) 858 Const. 35S prom. P941 4005 Altered glucosinolate composition; inc. M39481 G1202 AKR (105-619) 860 Const. 35S prom. P1383 4129 Inc. 18:0 and 18:1 fatty acids levels, decreased 18:3 fatty acids in leaves, inc. in leaf ?- carotene G1206 ENBP (209-255, 862 Const. 35S prom. P1713 4236 More tol. to 613-886) dehydration G1206 ENBP (209-255, 862 Const. 35S prom. P1713 4236 More tol. to drought* 613-886) and better recovery from drought treatment* G1225 HLH/MYC 864 Const. 35S prom. P1959 4291 Early flowering (82-141) G1225 HLH/MYC 864 Const. 35S prom. P1959 4291 Altered sugar sensing; (82-141) greater tol. to sucrose (determined in 9.4% sucrose) or 5% glucose media G1226 HLH/MYC 866 Const. 35S prom. P1393 4133 More seed oil content (109-168) G1226 HLH/MYC 866 2 comp. including P3647 4524 Significantly greater (109-168) P5284 (RBCS3 lycopene in tomato prom.) plants G1228 HLH/MYC 868 Const. 35S prom. P1195 4062 Smaller plants (172-231) G1229 HLH/MYC 870 Const. 35S prom. P946 4006 Less seed oil content (96-155) G1242 SWI/SNF 872 Const. 35S prom. P1209 4064 Early flowering (96-180, 417-466, 519-580) G1246 MYB- 874 Const. 35S prom. P1567 4191 Altered C/N sensing: (R1)R2R3 greater tol. to low (27-139) nitrogen conditions in C/N sensing assay G1247 MYB- 876 Const. 35S prom. P2795 4471 Altered leaf shape; (R1)R2R3 narrow, darker leaves (18-141) G1247 MYB- 876 Const. 35S prom. P2795 4471 Smaller plants (R1)R2R3 (18-141) G1249 CAAT (13-89) 880 Const. 35S prom. P1184 4059 Early flowering G1255 Z-CO-like 882 Const. 35S prom. P1500 4166 Inc. susceptibility to (19-57) Botrytis G1255 Z-CO-like 882 Const. 35S prom. P1500 4166 Inc. seed size (19-57) G1255 Z-CO-like 882 Const. 35S prom. P1500 4166 Reduced apical (19-57) dominance G1255 Z-CO-like 882 Const. 35S prom. P1500 4166 Altered C/N sensing: (19-57) greater tol. to low nitrogen conditions in C/N sensing assay G1266 AP2 (79-147) 884 Const. 35S prom. P483 3944 Greater resistance to Erysiphe G1266 AP2 (79-147) 884 Const. 35S prom. P483 3944 Reduced sens. to ABA G1266 AP2 (79-147) 884 Const. 35S prom. P483 3944 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1266 AP2 (79-147) 884 Const. 35S prom. P483 3944 Altered leaf insoluble sugars, including rhamnose, arabinose, xylose, and mannose, and galactose G1267 WRKY (70-127) 886 Const. 35S prom. P1453 4149 Smaller plant G1267 WRKY (70-127) 886 Const. 35S prom. P1453 4149 Darker green shiny leaves G1269 MYB- 888 Const. 35S prom. P484 3945 Altered light response; related (37-83) greater shade tol.; lack of shade avoidance phenotype; long petioles, upturned leaves G1272 PAZ (800-837) 890 Knockout not Decreased seed applicable glucosinolate M39497 G1273 WRKY 892 2 comp. including P3994 4536 Significantly greater (163-218, P5326 (AP1 lycopene in tomato 347-403) prom.) plants G1276 AP2 (158-224, 896 Const. 35S prom. P2402 4375 Late flowering 250-305) G1277 AP2 (18-85) 898 Const. 35S prom. P487 3947 Smaller plants G1289 AKR (90-578) 900 Const. 35S prom. P1384 4130 Smaller plant G1290 AKR (270-366) 902 Const. 35S prom. P1405 4137 Altered light response when overexpressed in tomato plants; greater shade tol.; lack of shade avoidance phenotype; long internodes G1304 MYB- 904 Const. 35S prom. P2022 4315 Lethal when (R1)R2R3 constitutively (13-118) overexpressed G1305 MYB- 906 Const. 35S prom. P2024 4317 Greater heat tol.; (R1)R2R3 reduced chlorosis in (15-118) heat (32 C.) G1305 MYB- 906 Const. 35S prom. P2024 4317 Early flowering (R1)R2R3 (15-118) G1309 MYB- 908 Const. 35S prom. P984 4018 Smaller plants (R1)R2R3 (13-115) G1309 MYB- 908 Const. 35S prom. P984 4018 Higher leaf mannose (R1)R2R3 level (13-115) G1311 MYB- 910 Const. 35S prom. P972 4014 Reduced fertility (R1)R2R3 (11-112) G1311 MYB- 910 Const. 35S prom. P972 4014 Smaller plants (R1)R2R3 (11-112) G1313 MYB- 912 Const. 35S prom. P2027 4320 Greater seedling size (R1)R2R3 (32-135) G1314 MYB- 914 Const. 35S prom. P701 3948 Reduced seedling vigor (R1)R2R3 on high glucose (14-116) G1314 MYB- 914 Const. 35S prom. P701 3948 Smaller plants (R1)R2R3 (14-116) G1317 MYB- 916 Const. 35S prom. P703 3949 Smaller plants (R1)R2R3 (13-118) G1322 MYB- 918 Const. 35S prom. P1560 4188 Greater seedling vigor (R1)R2R3 in cold (8 C.) (26-130) G1322 MYB- 918 Const. 35S prom. P1560 4188 Smaller plant (R1)R2R3 (26-130) G1322 MYB- 918 Const. 35S prom. P1560 4188 Altered leaf (R1)R2R3 glucosinolates; more (26-130) M39480 G1322 MYB- 918 Const. 35S prom. P1560 4188 Altered light response (R1)R2R3 and/or shade tol.; (26-130) constitutive photomorphogenesis, photomorphogenesis in the dark G1322 MYB- 918 Const. 35S prom. P1560 4188 Altered C/N sensing: (R1)R2R3 greater tol. to low (26-130) nitrogen conditions in C/N sensing assay G1323 MYB- 920 Const. 35S prom. P987 4020 Decreased seed oil (R1)R2R3 content (15-116) G1323 MYB- 920 Const. 35S prom. P987 4020 Greater seed protein (R1)R2R3 content (15-116) G1323 MYB- 920 Const. 35S prom. P987 4020 Small darker green T1 (R1)R2R3 plants (15-116) G1324 MYB- 922 Const. 35S prom. P707 3950 Lower leaf lutein, (R1)R2R3 higher leaf xanthophyll (20-118) levels G1324 MYB- 922 2 comp. including P4914 4591 Significantly greater (R1)R2R3 P5297 (PG prom.) lycopene in tomato (20-118) plants G1326 MYB- 924 Const. 35S prom. P709 3951 Petals and sepals are (R1)R2R3 smaller (18-121) G1326 MYB- 924 Const. 35S prom. P709 3951 Smaller plant (R1)R2R3 (18-121) G1326 MYB- 924 Const. 35S prom. P709 3951 Reduced fertility (R1)R2R3 (18-121) G1327 MYB- 926 Const. 35S prom. P15372 4700 Darker green leaves (R1)R2R3 (14-116) G1328 MYB- 928 Const. 35S prom. P711 3952 Decreased seed lutein (R1)R2R3 (14-119) G1328 MYB- 928 2 comp. including P3592 4515 Significantly greater (R1)R2R3 P5284 (RBCS3 plant volume in tomato (14-119) prom.) plants G1330 MYB- 930 Const. 35S prom. P986 4019 Ethylene insensitive (R1)R2R3 when germinated in the (28-134) dark on ACC G1331 MYB- 932 Const. 35S prom. P2020 4314 Altered light response; (R1)R2R3 greater shade tol.; lack (8-109) of shade avoidance phenotype;; constitutive photomorphogenesis G1331 MYB- 932 Const. 35S prom. P2020 4314 Altered C/N sensing: (R1)R2R3 greater tol. to low (8-109) nitrogen conditions in C/N sensing assay G1332 MYB- 934 Const. 35S prom. P2026 4319 Reduced trichome (R1)R2R3 density (13-116) G1332 MYB- 934 Const. 35S prom. P2026 4319 Altered C/N sensing: (R1)R2R3 much greater tol. to (13-116) low nitrogen conditions in C/N sensing assay G1332 MYB- 934 Const. 35S prom. P2026 4319 Smaller plants (R1)R2R3 (13-116) G1335 Z-CLDSH 938 Const. 35S prom. P715 3954 Late flowering (24-43, 131-144, 185-203) G1335 Z-CLDSH 938 Const. 35S prom. P715 3954 Slow growth (24-43, 131-144, 185-203) G1337 Z-CO-like 940 Const. 35S prom. P716 3955 Altered sugar sensing; (9-75) greater sens. to sucrose (determined in 9.4% sucrose) G1337 Z-CO-like 940 Const. 35S prom. P716 3955 Sharp increase in leaf (9-75) 18:0 fatty acid composition G1340 TH (54-142) 942 Const. 35S prom. P1264 4088 Smaller plants G1341 BZIPT2 (1-34, 944 Const. 35S prom. P15340 4693 Narrow, darker green 288-398) leaves, leaf curling G1357 NAC (17-158) 946 Const. 35S prom. P2775 4467 Altered leaves; rounder and darker green leaves G1357 NAC (17-158) 946 Const. 35S prom. P2775 4467 More tol. to growth in cold (8 C.) G1357 NAC (17-158) 946 Const. 35S prom. P2775 4467 Inc. tol. to drought* G1357 NAC (17-158) 946 Const. 35S prom. P2775 4467 Insensitive to ABA G1357 NAC (17-158) 946 Const. 35S prom. P2775 4467 Late flowering G1361 NAC (59-200) 948 Const. 35S prom. P3303 4492 Altered leaf shape; long, narrow leaves G1361 NAC (59-200) 948 Const. 35S prom. P3303 4492 Late flowering G1380 AP2 (24-91) 954 Const. 35S prom. P1056 4039 Early flowering G1382 WRKY 956 Const. 35S prom. P1187 4060 Smaller plants (210-266, 385-437) G1384 AP2 (127-194) 958 Const. 35S prom. P2117 4356 Lethal when constitutively overexpressed G1389 TEO (30-87) 962 Const. 35S prom. P1755 4250 Inner rosette leaves were darker green, narrow, and curled in T1 plants G1399 AT-hook 964 Const. 35S prom. P1076 4045 Inc in percentage of (86-94) 16:0 leaf fatty acids G1411 AP2 (87-154) 966 Const. 35S prom. P737 3957 Altered architecture; loss of apical dominance G1412 NAC (13-162) 968 Knockout not Altered light response; applicable greater shade tol.; lack of shade avoidance phenotype G1412 NAC (13-162) 968 Const. 35S prom. P15243 4690 Less sens. to ABA G1412 NAC (13-162) 968 Const. 35S prom. P15243 4690 Altered sugar sensing; greater tol. to sucrose (determined in 9.4% sucrose) G1417 WRKY 970 Knockout not Altered seed oil; inc. in (239-296) applicable 18:2, decrease in 18:3 fatty acids G1417 WRKY 970 Knockout not Reduced seedling (239-296) applicable germination and vigor G1419 AP2 (69-137) 972 Const. 35S prom. P1057 4040 Greater seed protein content G1420 WRKY 974 Const. 35S prom. P1211 4065 Long flower organs (221-280) (sepal and petal) G1420 WRKY 974 Const. 35S prom. P1211 4065 Altered leaf shape; (221-280) darker green leaves, including pedicel, mildly serrated, narrow, and rather contorted leaves G1420 WRKY 974 Const. 35S prom. P1211 4065 Altered sugar sensing; (221-280) poor growth on 5% glucose G1420 WRKY 974 Const. 35S prom. P1211 4065 Altered light response; (221-280) greater shade tol.; lack of shade avoidance phenotype; long narrow cotyledons G1421 AP2 (84-146) 976 Const. 35S prom. P1270 4092 Darker green leaves, altered leaf shape G1423 MADS (6-62) 978 Const. 35S prom. P2422 4383 Altered leaf coloration; darker green leaves G1423 MADS (6-62) 978 Const. 35S prom. P2422 4383 Smaller plants G1425 NAC (20-173) 980 Const. 35S prom. P1361 4119 Altered flower and inflorescence development; short internodes; flowers occasionally failed to open, or had reductions in organ size and poor anther dehiscence G1425 NAC (20-173) 980 Const. 35S prom. P1361 4119 Altered light response; greater shade tol.; lack of shade avoidance phenotype G1435 GARP (146-194) 982 Const. 35S prom. P745 3958 Late flowering G1435 GARP (146-194) 982 Const. 35S prom. P745 3958 Inc. biomass, greater plant size G1444 GRF-like 984 2 comp. including P4397 4559 Significantly greater (17-101) P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G1444 GRF-like 984 2 comp. including P4397 4559 Significantly greater (17-101) P6506 (35S prom.) tomato plant volume G1444 GRF-like 984 2 comp. including P4397 4559 Significantly greater (17-101) P5319 (ASA tomato plant volume prom.) G1446 MISC (1-405) 986 Const. 35S prom. P2377 4362 Late flowering G1449 IAA (48-53, 988 Const. 35S prom. P751 3959 Altered flower 74-107, structure; changes in 122-152) floral organ number and identity, large petals G1449 IAA (48-53, 988 Const. 35S prom. P751 3959 Higher seed protein 74-107, content 122-152) G1451 ARF (22-357) 990 Cons. 35S prom. P2617 4432 Inc. plant size, more biomass, larger leaves G1451 ARF (22-357) 990 Cons. 35S prom. P2617 4432 Late flowering G1451 ARF (22-357) 990 Knockout not Altered seed oil applicable content; inc. seed oil and protein combined content G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 Reduced trichome density G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 Altered leaf shape, darker green color G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 Less sens. to ABA G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 Late flowering G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 More tol. to hyperosmotic stress; better germination on 9.4% sucrose, or 150 mM NaCl G1452 NAC (55-196) 992 Const. 35S prom. P1537 4180 More tol. to drought* and better recovery from drought treatment* G1453 NAC (13-160) 994 Const. 35S prom. P1523 4175 At flowering time, many plants developed more slowly than controls and formed bushy inflorescence stems with narrow internodes between flowers, flowers poorly formed or had contorted organs, reduced fertility G1462 NAC (14-273) 996 2 comp. including P4336 4545 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G1462 NAC (14-273) 996 2 comp. including P4336 4545 Significantly greater P5326 (AP1 lycopene in tomato prom.) plants G1463 NAC (9-156) 998 Const. 35S prom. P1528 4176 Premature senescence G1463 NAC (9-156) 998 2 comp. including P4337 4546 Significantly greater P5284 (RBCS3 plant volume in tomato prom.) plants G1463 NAC (9-156) 998 2 comp. including P4337 4546 Significantly greater P5318 (STM plant volume in tomato prom.) plants G1465 NAC (242-306) 1000 Const. 35S prom. P1530 4177 Inc. leaf 16:0, 16:1, 18:0 and 18:2, and decreased 16:3 and 18:3 fatty acid content G1465 NAC (242-306) 1000 Const. 35S prom. P1530 4177 Reduced seed oil content G1466 PMR (154-420) 1002 Const. 35S prom. P753 3960 Higher seed oil content G1468 Z-C2H2 1004 Const. 35S prom. P15660 4733 Late flowering (95-115, 170-190) G1468 Z-C2H2 1004 Const. 35S prom. P15660 4733 Greater biomass; inc. (95-115, number of axillary 170-190) rosette leaves G1468 Z-C2H2 1004 Const. 35S prom. P15660 4733 Grayish and narrow (95-115, leaves 170-190) G1468 Z-C2H2 1004 Const. 35S prom. P15660 4733 Slow growth rate (95-115, 170-190) G1471 Z-C2H2 1006 Const. 35S prom. P1600 4199 More seed oil content (49-70) G1472 Z-C2H2 1008 Const. 35S prom. P2704 4451 No shoot meristem (83-106) G1474 Z-C2H2 1010 Const. 35S prom. P1602 4200 Smaller plants (41-68) G1474 Z-C2H2 1010 Const. 35S prom. P1602 4200 Late flowering (41-68) G1474 Z-C2H2 1010 Const. 35S prom. P1602 4200 Altered inflorescence (41-68) architecture; reduced internode elongation G1476 Z-C2H2 1012 Const. 35S prom. P1603 4201 Faster seedling growth (37-57) G1476 Z-C2H2 1012 Const. 35S prom. P1603 4201 Elongated cotyledons (37-57) G1476 Z-C2H2 1012 Const. 35S prom. P1603 4201 Smaller plants (37-57) G1478 Z-CO-like 1014 Const. 35S prom. P1605 4202 Decreased seed protein (32-76) content G1478 Z-CO-like 1014 Const. 35S prom. P1605 4202 Late flowering (32-76) G1478 Z-CO-like 1014 Const. 35S prom. P1605 4202 More seed oil content (32-76) G1480 Z-CO-like 1016 Const. 35S prom. P1606 4203 Early flowering (50-73, 92-116) G1481 Z-CO-like 1018 2 comp. including P4562 4575 Significantly greater (5-27, 47-73) P5284 (RBCS3 soluble solids (Brix) in prom.) tomato plants G1482 Z-CO-like 1020 Const. 35S prom. P1964 4294 Higher leaf (5-63) anthocyanin level G1482 Z-CO-like 1020 Knockout not More root growth, (5-63) applicable which is anticipated to increase yield; can be achieved through knockout or knock- down approaches against G1482 putative orthologs from target crops, e.g., by antisense RNAi TILLING or homology driven gene replacement strategies G1483 Z-CO-like 1022 Const. 35S prom. P15499 4715 Altered C/N sensing: (17-66) greater sens. to low nitrogen conditions in C/N sensing assay G1488 GATA/Zn 1024 Const. 35S prom. P1099 4057 Inc. total seed protein (221-246) and oil content G1488 GATA/Zn 1024 Const. 35S prom. P1099 4057 Altered light response; (221-246) greater shade tol.; lack of shade avoidance phenotype; constitutive photomorphogenesis G1488 GATA/Zn 1024 Const. 35S prom. P1099 4057 Reduced apical (221-246) dominance, shorter stems G1493 GARP (242-289) 1026 Const. 35S prom. P2619 4433 Altered sugar sensing; greater seedling vigor on 5% glucose G1493 GARP (242-289) 1026 Const. 35S prom. P2619 4433 Late flowering G1493 GARP (242-289) 1026 Const. 35S prom. P2619 4433 Altered light response; greater shade tol.; lack of shade avoidance phenotype; greater petiole length G1494 HLH/MYC 1028 Const. 35S prom. P961 4007 Early flowering (254-311) G1494 HLH/MYC 1028 Const. 35S prom. P961 4007 Altered light response; (254-311) greater shade tol.; lack of shade avoidance phenotype; long hypocotyls, elongated cotyledon petioles, rosette leaves were generally very pale, narrow, upward pointing, and had long petioles G1494 HLH/MYC 1028 Const. 35S prom. P961 4007 Internodes between (254-311) rosette leaves extended (long internodes) G1494 HLH/MYC 1028 Const. 35S prom. P961 4007 Altered C/N sensing: (254-311) greater tol. to low nitrogen conditions in C/N sensing assay G1496 HLH/MYC 1030 Const. 35S prom. P1005 4026 Altered seed oil (188-246) content G1499 HLH/MYC 1032 Const. 35S prom. P1240 4079 Altered pigment; (122-179) darker green color G1499 HLH/MYC 1032 Const. 35S prom. P1240 4079 Altered plant (122-179) architecture; inflorescence bolts that terminated without an inflorescence G1499 HLH/MYC 1032 Const. 35S prom. P1240 4079 Altered floral organ (122-179) identity and development; in some cases, flowers were replaced with filamentous structures or carpelloid structures. Less severely affected lines produced flowers where sepals were converted to carpelloid tissue G1504 GATA/Zn 1034 2 comp. including P4350 4548 Significantly greater (193-206) P5318 (STM plant volume in tomato prom.) plants G1506 GATA/Zn 1036 Const. 35S prom. P1254 4084 Inc. in seed (7-33) glucosinolate M39502 and M39498 G1510 GATA/Zn 1038 Const. 35S prom. P15051 4669 Darker green leaves (230-263) G1510 GATA/Zn 1038 Const. 35S prom. P15051 4669 Altered light response; (230-263) greater shade tol.; lack of shade avoidance phenotype; longer hypocotyls G1512 RING/C3HC4 1040 Const. 35S prom. P1468 4154 Decreased seed oil, (39-93) decreased seed 18:1 fatty acid content, inc. seed 18:2 fatty acid, inc. leaf 18:2 fatty acid content G1517 RING/C3HC4 1042 Const. 35S prom. P1096 4056 Altered light response; (312-349) greater shade tol.; lack of shade avoidance phenotype G1519 RING/C3HC4 1044 Knockout not Embryo lethal (327-364) applicable phenotype: potential herbicide target G1521 RING/C3HC4 1046 Const. 35S prom. P1420 4143 Altered light response; (39-80) greater shade tol.; lack of shade avoidance phenotype; constitutive photomorphogenesis, in the dark cotyledons partially expand as if the plant is grown in the light G1526 SWI/SNF 1048 Knockout not More seed oil content (344-641, applicable 794-833, 893-976) G1531 RING/C3HC4 1050 Const. 35S prom. P1541 4182 Round leaves and (41-77) bushy compact inflorescence G1535 HB (109-169) 1052 Knockout not Altered C/N sensing: applicable greater tol. to low nitrogen conditions in C/N sensing assay G1535 HB (109-169) 1052 Const. 35S prom. P2726 4456 Slow growth rate G1535 HB (109-169) 1052 Const. 35S prom. P2726 4456 Altered leaves; smaller, narrower and darker green leaves G1535 HB (109-169) 1052 Const. 35S prom. P2726 4456 Altered sugar sensing; larger, darker green seedlings with higher germination efficiency on 5% glucose G1535 HB (109-169) 1052 Const. 35S prom. P2726 4456 Altered C/N sensing: greater sens. to low nitrogen conditions in C/N sensing assay G1537 HB (14-74) 1054 Const. 35S prom. P1047 4036 Inc. 18:1 leaf fatty acid levels G1538 HB (66-126) 1056 Const. 35S prom. P1048 4037 Greater tol. to NaCl (determined with 150 mM NaCl) G1538 HB (66-126) 1056 Const. 35S prom. P1048 4037 Altered light response; greater shade tol.; lack of shade avoidance phenotype; longer leaf petioles G1538 HB (66-126) 1056 Const. 35S prom. P1048 4037 Early flowering G1539 HB (76-136) 1058 Const. 35S prom. P2727 4457 Altered trichome structure; stem outgrowths in 1 line developed a trichome at their apex G1539 HB (76-136) 1058 Const. 35S prom. P2727 4457 Altered cell differentiation; patches of callus-like tissue on the stems and flower pedicels, and these appeared to partially differentiate with a carpelloid identity G1539 HB (76-136) 1058 Const. 35S prom. P2727 4457 Ectopic carpel development; in the inflorescence, growths developed from stems, pedicels and floral organs and took on a carpelloid identity G1540 HB (35-98) 1060 Const. 35S prom. P756 3961 Reduced cell differentiation in meristem G1545 HB (54-117) 1064 Const. 35S prom. P758 3962 Early flowering G1545 HB (54-117) 1064 Const. 35S prom. P758 3962 Smaller plants G1549 HB (75-135) 1066 Const. 35S prom. P2728 4458 Smaller plants G1549 HB (75-135) 1066 Const. 35S prom. P2728 4458 Slow growth rate G1549 HB (75-135) 1066 Const. 35S prom. P2728 4458 Late flowering G1549 HB (75-135) 1066 Const. 35S prom. P2728 4458 Altered leaf shape and coloration; serrated, darker leaves G1554 GARP (238-287) 1068 Const. 35S prom. P13386 4616 Late flowering G1554 GARP (238-287) 1068 Const. 35S prom. P13386 4616 Darker leaves G1556 GARP (19-67) 1070 Const. 35S prom. P16178 4739 Lethal when constitutively overexpressed G1557 GARP (19-67) 1072 Const. 35S prom. P13804 4643 Greater tol. to NaCl (determined with 150 mM NaCl) G1560 HS (61-152) 1074 Const. 35S prom. P1787 4260 Reduced fertility G1560 HS (61-152) 1074 Const. 35S prom. P1787 4260 Smaller plants G1560 HS (61-152) 1074 Const. 35S prom. P1787 4260 Abnormal flowers; petals and stamens, are poorly developed or absent, and flower buds are generally smaller and round- shaped G1585 HB (55-115) 1076 Const. 35S prom. P13394 4620 Altered cell differentiation; shoots initiated from the adaxial cotyledon surfaces G1585 HB (55-115) 1076 Const. 35S prom. P13394 4620 Altered leaf shape; upright, serrated G1587 HB (61-121) 1078 Const. 35S prom. P1968 4296 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1591 HB (8-68) 1080 Const. 35S prom. P16175 4737 Altered cell differentiation; filamentous carpelloid growths developed on flower pedicels G1591 HB (8-68) 1080 Const. 35S prom. P16175 4737 Altered leaf shape; narrow dark contorted leaves G1593 HB (227-290) 1082 Const. 35S prom. P2732 4459 Altered inflorescence architecture; shorter compact inflorescences, which had reduced internode elongation, and flowers bunched together at the tips, larger flowers G1593 HB (227-290) 1082 Const. 35S prom. P2732 4459 Altered leaf shape and coloration; dark, and lobes sometimes apparent in the leaf margins G1594 HB (308-343) 1084 Const. 35S prom. P1967 4295 Pale, large seed G1634 MYB- 1086 Const. 35S prom. P760 3963 More seed oil content related (29-79, 131-179) G1634 MYB- 1086 Const. 35S prom. P760 3963 Decreased seed protein related (29-79, content 131-179) G1635 MYB- 1088 Const. 35S prom. P988 4021 Reduced apical related (56-102) dominance G1635 MYB- 1088 Const. 35S prom. P988 4021 Reduced bolt related (56-102) elongation G1635 MYB- 1088 2 comp. including P3606 4520 Significantly greater related (56-102) P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G1635 MYB- 1088 2 comp. including P3606 4520 Significantly greater related (56-102) P5297 (PG prom.) soluble solids (Brix) in tomato plants G1635 MYB- 1088 2 comp. including P3606 4520 Significantly greater related (56-102) P5318 (STM lycopene in tomato prom.) plants G1635 MYB- 1088 2 comp. including P3606 4520 Significantly greater related (56-102) P5326 (AP1 tomato plant volume prom.) G1635 MYB- 1088 2 comp. including P3606 4520 Significantly greater related (56-102) P5303 (PD prom.) tomato plant volume G1637 MYB- 1090 Const. 35S prom. P991 4023 Altered seed protein related (108-156) content G1638 MYB- 1092 2 comp. including P3843 4530 Significantly greater related (27-77, P5297 (PG prom.) lycopene in tomato 141-189) plants G1640 MYB- 1094 Const. 35S prom. P983 4017 Greater seed oil (R1)R2R3 content (14-115) G1640 MYB- 1094 2 comp. including P3604 4519 Significantly greater (R1)R2R3 P5319 (AS1 tomato plant volume (14-115) prom.) G1641 MYB- 1096 Const. 35S prom. P1450 4148 Inc. leaf rhamnose, related (32-82, decreased leaf 141-189) arabinose, inc. seed glucosinolate M39489 G1641 MYB- 1096 Const. 35S prom. P1450 4148 Pale, spindly stems related (32-82, 141-189) G1645 MYB- 1098 Const. 35S prom. P1619 4209 Altered inflorescence (R1)R2R3 structure; reduced (90-210) apical dominance, flowers were frequently abnormal and had organs missing, reduced in size, or contorted, pollen production also appeared poor G1645 MYB- 1098 Const. 35S prom. P1619 4209 Altered leaf (R1)R2R3 development; leaves (90-210) misshapen and highly contorted G1645 MYB- 1098 Const. 35S prom. P1619 4209 Reduced germination (R1)R2R3 vigor (90-210) G1645 MYB- 1098 2 comp. including P4387 4554 Significantly greater (R1)R2R3 P5297 (PG prom.) soluble solids (Brix) in (90-210) tomato plants G1649 HLH/MYC 1102 Const. 35S prom. P1960 4292 Altered C/N sensing: (226-283) greater tol. to low nitrogen conditions in C/N sensing assay G1650 HLH/MYC 1104 2 comp. including P3979 4534 Significantly greater (274-331) P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G1650 HLH/MYC 1104 2 comp. including P3979 4534 Significantly greater (274-331) P5326 (AP1 tomato plant volume prom.) G1652 HLH/MYC 1106 Const. 35S prom. P1302 4103 More seed protein (147-204) content G1655 HLH/MYC 1108 Const. 35S prom. P1008 4027 Altered light response; (129-186) greater shade tol.; lack of shade avoidance phenotype G1660 DBP (362-476) 1110 Const. 35S prom. P2443 4392 Greater tol. to NaCl (determined with 150 mM NaCl) G1662 DBP (44-69, 1112 Const. 35S prom. P1961 4293 Altered light response 295-330) when overexpressed in tomato plants; greater shade tol.; lack of shade avoidance phenotype; long internodes G1666 HLH/MYC 1114 Knockout not Altered C/N sensing: (356-413) applicable greater tol. to low nitrogen conditions in C/N sensing assay G1666 HLH/MYC 1114 Knockout not Pale seeds, indicating (356-413) applicable this TF is a regulator of pigments such as flavonoids G1672 NAC (41-194) 1118 Const. 35S prom. P1073 4043 Altered seed oil content G1677 NAC (17-181) 1120 Const. 35S prom. P1074 4044 Altered seed protein G1677 NAC (17-181) 1120 Const. 35S prom. P1074 4044 Altered seed oil content G1700 RING/C3H2C3 1122 Knockout not Altered C/N sensing: (93-134) applicable greater tol. to low nitrogen conditions in C/N sensing assay G1706 RING/C3H2C3 1124 Knockout not Early flowering (180-212) applicable G1718 RING/C3H2C3 1126 Const. 35S prom. P15343 4695 Altered leaf coloration; (113-153) pale gray leaves G1730 RING/C3H2C3 1128 Const. 35S prom. P15024 4660 Inc. tol. to (103-144) hyperosmotic stress; seedlings more tol. to 300 mM mannitol G1730 RING/C3H2C3 1128 Const. 35S prom. P15024 4660 Altered sugar sensing; (103-144) seedlings larger, greener and had higher germination efficiency in 5% glucose G1730 RING/C3H2C3 1128 Const. 35S prom. P15024 4660 More tol. to drought* (103-144) G1743 RING/C3H2C3 1130 Const. 35S prom. P15028 4661 Altered light response; (94-136) greater shade tol.; lack of shade avoidance phenotype G1743 RING/C3H2C3 1130 Const. 35S prom. P15028 4661 Altered inflorescence (94-136) architecture; inflorescences had short internodes, which led to a more compact bushier architecture G1743 RING/C3H2C3 1130 Const. 35S prom. P15028 4661 Altered leaf shape, (94-136) darker green rounded leaves with short petioles G1749 AP2 (84-152) 1132 Const. 35S prom. P1457 4150 Altered necrosis; more formation of necrotic lesions G1750 AP2 (115-177) 1134 Const. 35S prom. P1034 4033 More seed oil content G1750 AP2 (115-177) 1134 Const. 35S prom. P1034 4033 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G1750 AP2 (115-177) 1134 Const. 35S prom. P1034 4033 Greater resistance to Erysiphe G1755 AP2 (71-133) 1140 2 comp. including P4407 4563 Significantly greater P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G1755 AP2 (71-133) 1140 2 comp. including P4407 4563 Significantly greater P5303 (PD prom.) soluble solids (Brix) in tomato plants G1755 AP2 (71-133) 1140 2 comp. including P4407 4563 Significantly greater P5303 (PD prom.) lycopene in tomato plants G1755 AP2 (71-133) 1140 2 comp. including P4407 4563 Significantly greater P5303 (PD prom.) tomato plant volume G1755 AP2 (71-133) 1140 2 comp. including P4407 4563 Significantly greater P5297 (PG prom.) tomato plant volume G1756 WRKY 1142 Const. 35S prom. P1377 4127 Inc. susceptibility to (138-200) Botrytis G1759 MADS (2-57) 1146 Const. 35S prom. P1308 4105 Altered sugar sensing; reduced germination and seedling vigor on 5% glucose G1765 NAC (20-140) 1148 Const. 35S prom. P1534 4179 Higher seed oil content G1767 SCR (225-290, 1150 Const. 35S prom. P1476 4156 Early flowering 355-450, 453-528) G1772 RING/C3HC4 1152 Const. 35S prom. P13862 4651 Smaller plants (123-176) G1773 RING/C3HC4 1154 Knockout not Altered C/N sensing: (139-184) applicable greater tol. to low nitrogen conditions in C/N sensing assay G1777 RING/C3HC4 1156 Const. 35S prom. P1554 4184 More seed oil content (124-247) G1777 RING/C3HC4 1156 Const. 35S prom. P1554 4184 Decreased seed protein (124-247) content G1779 GATA/Zn 1158 Const. 35S prom. P1726 4242 More tol. to cold (190-239) during growth (8 C.) G1784 PMR (60-248) 1164 2 comp. including P4035 4540 Significantly greater P5324 (Cru prom.) soluble solids (Brix) in tomato plants G1785 MYB- 1166 2 comp. including P4195 4542 Significantly greater (R1)R2R3 P5324 (Cru prom.) soluble solids (Brix) in (25-125) tomato plants G1786 MYB- 1168 Const. 35S prom. P1279 4096 Dark green, small (R1)R2R3 leaves with short (NA) petioles G1786 MYB- 1168 Const. 35S prom. P1279 4096 Altered light response; (R1)R2R3 greater shade tol.; lack (NA) of shade avoidance phenotype, constitutive morphogenesis G1789 MYB- 1170 Const. 35S prom. P1562 4189 Altered light response; related (12-62) greater shade tol.; lack of shade avoidance phenotype G1793 AP2 (179-255, 1174 Const. 35S prom. P1506 4168 Higher seed oil content 281-349) G1794 AP2 (182-249) 1176 Const. 35S prom. P2051 4330 Altered architecture, bushier plant, reduced apical dominance, very thick hypocotyls G1794 AP2 (182-249) 1176 Const. 35S prom. P2051 4330 Altered light response; greater shade tol.; lack of shade avoidance phenotype; constitutive photomorphogenesis, seedlings have open cotyledons and more root growth in the dark G1794 AP2 (182-249) 1176 Const. 35S prom. P2051 4330 More sensitive to PEG G1794 AP2 (182-249) 1176 Const. 35S prom. P2051 4330 Reduced root growth G1796 AP2 (54-121) 1178 Const. 35S prom. P2053 4332 Altered light response; greater shade tol.; lack of shade avoidance phenotype G1796 AP2 (54-121) 1178 Const. 35S prom. P2053 4332 Flower carpel alterations (thickened club-like carpels) G1796 AP2 (54-121) 1178 Const. 35S prom. P2053 4332 Short floral internodes G1796 AP2 (54-121) 1178 Const. 35S prom. P2053 4332 Dark curled leaves G1797 MADS (1-57) 1180 Const. 35S prom. P15510 4717 Early flowering G1797 MADS (1-57) 1180 Const. 35S prom. P15510 4717 Flower organs persisted following fertilization G1798 MADS (1-57) 1182 Const. 35S prom. P13690 4630 Early flowering G1798 MADS (1-57) 1182 Const. 35S prom. P13690 4630 Terminal flowers and floral organs were often reduced in size, stamens were typically short, and pollen production was very poor G1804 bZIP (357-407) 1184 Const. 35S prom. P1086 4050 Late flowering G1804 bZIP (357-407) 1184 Const. 35S prom. P1086 4050 Altered sugar sensing: more sensitive to 5% glucose in germination assays G1806 bZIP (165-225) 1186 Const. 35S prom. P1559 4187 Altered light response; greater shade tol.; lack of shade avoidance phenotype G1808 bZIP (140-200) 1188 Const. 35S prom. P1933 4282 More sens. to cold (8 C.) G1808 bZIP (140-200) 1188 2 comp. including P4601 4580 Significantly greater P5284 (RBCS3 soluble solids (Brix) in prom.) tomato plants G1809 bZIP (136-196) 1190 2 comp. including P3982 4535 Significantly greater P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G1815 MYB- 1192 2 comp. including P4728 4583 Significantly greater (R1)R2R3 P6506 (35S prom.) tomato plant volume (65-170) G1823 GARP (205-252) 1204 Const. 35S prom. P2616 4431 Early flowering G1825 GARP (55-103) 1206 Const. 35S prom. P13789 4639 Early flowering G1825 GARP (55-103) 1206 Const. 35S prom. P13789 4639 Altered leaf shape; flat rosette and cauline leaves that had mild serrations on the margins G1832 Z-C2H2 1208 Const. 35S prom. P2663 4446 Lethal when (67-87, 150-166, constitutively 213-233) overexpressed G1835 GATA/Zn 1210 Const. 35S prom. P1549 4183 Altered C/N sensing: (224-296) accumulated more anthocyanin in C/N sensing assay G1837 BZIPT2 (1-53, 1214 Const. 35S prom. P2473 4402 Greater tol. to NaCl 398-507) (determined with 150 mM NaCl) G1837 BZIPT2 (1-53, 1214 Const. 35S prom. P2473 4402 More tol. to cold (8 C.) 398-507) G1838 AP2 (230-304, 1216 Const. 35S prom. P1578 4195 More seed oil content 330-400) G1839 AP2 (118-182) 1218 Const. 35S prom. P1376 4126 Decreased apical dominance G1840 AP2 (87-154) 1220 Const. 35S prom. P15088 4676 Necrosis and death of patches of tissue induced in aerial part of the plant G1841 AP2 (83-150) 1222 Const. 35S prom. P1477 4157 Better germination under heat stress (32 C.) G1841 AP2 (83-150) 1222 Const. 35S prom. P1477 4157 Early flowering G1842 MADS (2-57) 1224 Const. 35S prom. P1685 4221 Early flowering G1843 MADS (2-57) 1226 Const. 35S prom. P1689 4224 Early flowering G1844 MADS (2-57) 1228 Const. 35S prom. P1690 4225 Early flowering G1844 MADS (2-57) 1228 Knockout not Early flowering applicable G1846 AP2 (16-83) 1230 Const. 35S prom. P2118 4357 Darker green leaves, poorly developed inflorescences G1850 HS (59-150) 1232 Const. 35S prom. P1399 4135 Lethal when constitutively overexpressed G1852 AKR (90-590) 1234 Const. 35S prom. P1401 4136 Better root growth under hyperosmotic stress in PEG G1855 AKR (102-613) 1236 Const. 35S prom. P1970 4297 Darker green G1855 AKR (102-613) 1236 Const. 35S prom. P1970 4297 Late flowering, late bolting, late senescing G1863 GRF-like 1238 Const. 35S prom. P1407 4139 Altered leaf shape, (76-187) larger leaves, and dark coloration G1863 GRF-like 1238 Const. 35S prom. P1407 4139 Late flowering (76-187) G1863 GRF-like 1238 Knockout not Greater sens. to NaCl (76-187) applicable (determined with 150 mM NaCl) G1865 GRF-like 1240 Const. 35S prom. P1387 4131 Darker leaves (45-162) G1865 GRF-like 1240 Const. 35S prom. P1387 4131 Broad leaves and (45-162) greatly increased number of leaves G1865 GRF-like 1240 2 comp. including P3645 4523 Significantly greater (45-162) P5324 (Cru prom.) tomato plant volume G1868 GRF-like 1242 Const. 35S prom. P1388 4132 Altered C/N sensing: (164-270) greater tol. to low nitrogen conditions in C/N sensing assay G1880 Z-C2H2 1244 Knockout not Greater resistance to (69-89, 111-139) applicable Botrytis G1884 Z-Dof (43-71) 1246 2 comp. including P4563 4576 Significantly greater P5287 (LTP1 lycopene in tomato prom.) plants G1888 Z-CO-like 1248 Const. 35S prom. P1496 4162 Smaller plant, darker (5-50) green leaves G1888 Z-CO-like 1248 Const. 35S prom. P1496 4162 Altered C/N sensing: (5-50) accumulated more anthocyanin in C/N sensing assay G1893 Z-C2H2 1250 Const. 35S prom. P2804 4474 Less sens. to ABA (73-185) G1893 Z-C2H2 1250 Const. 35S prom. P2804 4474 Seedlings contained (73-185) more anthocyanin G1893 Z-C2H2 1250 Const. 35S prom. P2804 4474 Altered leaf shape; (73-185) leaves were small with serrated margins, rectangular cotyledons G1895 Z-Dof (58-100) 1252 Const. 35S prom. P1778 4258 Late flowering G1895 Z-Dof (58-100) 1252 2 comp. including P4546 5106 Significantly greater P5326 (AP1 tomato plant volume prom.) G1895 Z-Dof (58-100) 1252 2 comp. including P4546 5106 Significantly greater P5319 (AS1 tomato plant volume prom.) G1897 Z-Dof (34-62) 1254 2 comp. including P4547 4573 Significantly greater P5324 (Cru prom.) tomato plant volume G1900 Z-Dof (54-106) 1256 Const. 35S prom. P1022 4029 Late flowering G1902 Z-Dof (31-59) 1258 Const. 35S prom. P1059 4041 More seed oil content G1903 Z-Dof (134-180) 1260 Const. 35S prom. P1060 4042 Decreased seed protein content G1903 Z-Dof (134-180) 1260 2 comp. including P3617 4521 Significantly greater P5287 (LTP1 lycopene in tomato prom.) plants G1903 Z-Dof (134-180) 1260 2 comp. including P3617 4521 Significantly greater P5324 (Cru prom.) tomato plant volume G1903 Z-Dof (134-180) 1260 2 comp. including P3617 4521 Significantly greater P5287 (LTP1 tomato plant volume prom.) G1909 Z-Dof (23-51) 1262 2 comp. including P4529 4571 Significantly greater P5324 (Cru prom.) tomato plant volume G1911 MYB- 1264 Const. 35S prom. P989 4022 Altered light response; related (12-62) greater shade tol.; lack of shade avoidance phenotype G1917 GATA/Zn 1266 Const. 35S prom. P1584 4198 Altered leaf shape; (153-179) leaves elongated and curled; with frilly, serrated margins G1919 RING/C3HC4 1268 Const. 35S prom. P1581 4196 Greater resistance to (214-287) Botrytis G1927 NAC (17-188) 1270 Const. 35S prom. P2029 4321 Greater resistance to Sclerotinia G1928 Z-C2H2 1272 Const. 35S prom. P16190 4741 Inc. tol. to cold (8 C.) (101-121, 178-198) G1929 Z-CO-like 1274 Const. 35S prom. P1772 4256 Darker green (31-53) G1929 Z-CO-like 1274 Const. 35S prom. P1772 4256 Later bolting, later (31-53) flowering, later senescing G1932 AP2 (9-71) 1278 Const. 35S prom. P2419 4382 Altered leaf shape; leaves were darker green with jagged leaf margins G1935 MADS (1-57) 1280 2 comp. including P4393 4556 Significantly greater P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G1936 PCF (64-129) 1282 Knockout not Greater susceptibility applicable to Sclerotinia G1936 PCF (64-129) 1282 Knockout not Greater susceptibility applicable to Botrytis G1938 PCF (74-143) 1284 Const. 35S prom. P1492 4161 Altered leaf shape; leaves curled, contorted G1938 PCF (74-143) 1284 Const. 35S prom. P1492 4161 Darker green leaves G1938 PCF (74-143) 1284 Const. 35S prom. P1492 4161 Slow growth rate G1938 PCF (74-143) 1284 Const. 35S prom. P1492 4161 More sensitive to osmotic stress G1944 AT-hook 1286 Const. 35S prom. P1305 4104 Early senescence (89-97) G1944 AT-hook 1286 Const. 35S prom. P1305 4104 35S::G1944 (89-97) Arabidopsis lines exhibited changes in plant size (reduced overall plant size), accelerated senescence and altered ethylene responses (35S::G1944 lines were more severely stunted in an ethylene insensitivity assay than wild-type, suggesting that G1944 may be involved in the ethylene signal transduction pathway), which together indicate that G1944 regulates components of energy metabolism; thus, G1944 overexpression can be expected to improve yield and quality, can be expected, for example, with a conditional or developmentally regulated promoter, to trigger the onset of senescence; and can be expected to increase leaf respiration and produce an increase in organic acids that act as precursors for osmolytes responsible for maintaining turgor and photosynthesis G1946 HS (37-128) 1288 Const. 35S prom. P1788 4261 More seed oil content G1946 HS (37-128) 1288 Const. 35S prom. P1788 4261 Decreased seed protein content G1946 HS (37-128) 1288 Const. 35S prom. P1788 4261 Early flowering G1946 HS (37-128) 1288 Const. 35S prom. P1788 4261 Greater root growth on phosphate-free media G1947 HS (19-110) 1290 Knockout not Reduced fertility applicable G1947 HS (19-110) 1290 Knockout not Extended period of applicable flowering G1948 AKR (1-367) 1292 Const. 35S prom. P1657 4217 More seed oil content G1948 AKR (1-367) 1292 Const. 35S prom. P1657 4217 Early development G1950 AKR (65-228) 1294 Const. 35S prom. P1406 4138 Greater resistance to Botrytis G1950 AKR (65-228) 1294 2 comp. including P3651 4526 Significantly greater P5326 (AP1 tomato plant volume prom.) G1950 AKR (65-228) 1294 2 comp. including P3651 4526 Significantly greater P5287 (LTP1 tomato plant volume prom.) G1950 AKR (65-228) 1294 2 comp. including P3651 4526 Significantly greater P5297 (PG prom.) tomato plant volume G1950 AKR (65-228) 1294 2 comp. including P3651 4526 Significantly greater P5303 (PD prom.) tomato plant volume G1954 HLH/MYC 1296 2 comp. including P4417 4564 Significantly greater (191-250) P5326 (AP1 soluble solids (Brix) in prom.) tomato plants G1957 ABI3/VP-1 1298 Const. 35S prom. P13803 4642 Lethal due to meristem (52-143) defects G1958 GARP (230-278) 1300 Knockout not Smaller plant and root applicable mass G1958 GARP (230-278) 1300 Knockout not More seed oil content applicable G1958 GARP (230-278) 1300 Knockout not Greater seed protein applicable content. G1958 GARP (230-278) 1300 2 comp. including P3663 4528 Significantly greater P6506 (35S prom.) tomato plant volume G1958 GARP (230-278) 1300 2 comp. including P3663 4528 Significantly greater P5319 (AS1 tomato plant volume prom.) G1958 GARP (230-278) 1300 2 comp. including P3663 4528 Significantly greater P5324 (Cru prom.) tomato plant volume G1965 Z-Dof (27-55) 1302 Const. 35S prom. P1028 4030 Lethal when constitutively overexpressed G1968 Z-C2H2 1304 Const. 35S prom. P2647 4441 More tol. to cold (8 C.) (64-84, 368-390) G1968 Z-C2H2 1304 Const. 35S prom. P2647 4441 Altered C/N sensing: (64-84, 368-390) greater sens. to low nitrogen conditions in C/N sensing assay G1983 Z-C3H (71-147) 1306 Const. 35S prom. P2401 4374 Darker green leaves G1983 Z-C3H (71-147) 1306 Const. 35S prom. P2401 4374 Smaller plants G1983 Z-C3H (71-147) 1306 Const. 35S prom. P2401 4374 Late flowering G1985 Z-C2H2 1308 Const. 35S prom. P2643 4439 Phase change and (37-57) floral reversion; inflorescence meristem apparently reverted back to initiating leaf primordia once it entered flower initiation phase G1985 Z-C2H2 1308 Const. 35S prom. P2643 4439 Aerial rosettes (37-57) G1990 Z-C2H2 1310 Const. 35S prom. P15567 4723 Lethal when (184-204, constitutively 261-283) overexpressed G1993 Z-C2H2 1312 Const. 35S prom. P2641 4438 Short petioles and (23-43) round leaf shape G1993 Z-C2H2 1312 Const. 35S prom. P2641 4438 Smaller plants (23-43) G1995 Z-C2H2 1314 Const. 35S prom. P2360 4359 Altered light response; (93-113) greater shade tol.; lack of shade avoidance phenotype G1995 Z-C2H2 1314 Const. 35S prom. P2360 4359 Greater trichome (93-113) number on sepals, ectopic trichomes on carpels yield enhanced production of leaf, flower, and outer ovule epidermis products G1995 Z-C2H2 1314 Const. 35S prom. P2360 4359 Slightly less tol. to low (93-113) nitrogen or low phosphorus G1995 Z-C2H2 1314 Const. 35S prom. P2360 4359 Aerial rosettes (93-113) occurred when a secondary inflorescence meristem developed in a manner comparable to a primary shoot meristem during the vegetative phase of growth, with aerial rosette-like structures and floral organs being bract-like G1998 Z-CO-like 1316 Const. 35S prom. P2505 4410 Late flowering (5-71) G1999 Z-CO-like 1318 Const. 35S prom. P2501 4408 Late flowering (15-55) G2007 MYB- 1320 Const. 35S prom. P1678 4220 Late flowering (R1)R2R3 (14-118) G2010 SBP (53-127) 1322 Const. 35S prom. P1278 4095 Early flowering G2011 HS (55-146) 1324 Const. 35S prom. P1813 4264 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2030 AKR (30-130) 1326 Const. 35S prom. P1797 4263 Early flowering G2035 AKR (58-259) 1328 Const. 35S prom. P13693 4631 Greater tol. to NaCl (determined with 150 mM NaCl) G2041 SWI/SNF 1330 Const. 35S prom. P13846 4649 Greater tol. to NaCl (670-906, (determined with 150 mM 1090-1175) NaCl) G2051 NAC (7-158) 1332 Const. 35S prom. P15643 4731 Greater tol. to cold (8 C.) G2052 NAC (7-158) 1334 2 comp. including P4423 4567 Significantly greater P5326 (AP1 tomato plant volume prom.) G2052 NAC (7-158) 1334 2 comp. including P4423 4567 Significantly greater P5303 (PD prom.) tomato plant volume G2052 NAC (7-158) 1334 2 comp. including P4423 4567 Significantly greater P5287 (LTP1 tomato plant volume prom.) G2057 TEO (46-103) 1338 Const. 35S prom. P1089 4051 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G2059 AP2 (184-251) 1340 Const. 35S prom. P1482 4159 Altered seed oil and protein content; inc. seed oil and protein combined content G2060 WRKY 1342 Const. 35S prom. P1294 4100 Greater tol. to NaCl (204-263) (determined with 150 mM NaCl) G2063 MADS (7-63) 1344 Const. 35S prom. P2074 4341 Greater tol. to cold (8 C.) G2070 bZIP (45-137) 1348 Const. 35S prom. P1935 4283 Greater sens. to cold (8 C.) G2071 bZIP (307-358) 1350 Const. 35S prom. P13443 4628 Early flowering G2072 bZIP (90-149) 1352 2 comp. including P4603 4581 Significantly greater P5319 (AS1 lycopene in tomato prom.) plants G2084 RING/C3HC4 1354 Const. 35S prom. P1582 4197 Altered leaf shape; (41-172) short petioles, and rounded, slightly darker green leaves G2085 GATA/Zn 1356 Const. 35S prom. P1725 4241 Inc. seed size and (214-241) altered seed color G2085 GATA/Zn 1356 Const. 35S prom. P1725 4241 Greater trichome (214-241) density G2085 GATA/Zn 1356 Const. 35S prom. P1725 4241 Small darker green (214-241) leaves G2094 GATA/Zn 1358 Const. 35S prom. P1839 4270 Inc. leaf arabinose (43-68) G2105 TH (100-153) 1360 Const. 35S prom. P1937 4284 Large, pale seeds G2106 AP2 (56-139, 1362 Const. 35S prom. P13733 4635 Late flowering 165-233) G2107 AP2 (27-94) 1364 Const. 35S prom. P1831 4267 Darker green G2107 AP2 (27-94) 1364 Const. 35S prom. P1831 4267 Greater tol. to mannitol (300 mM) G2107 AP2 (27-94) 1364 2 comp. including P7170 4603 Darker green P5486 (35S prom., 35S::oEnh::LexA GaL4::TA::GR) G2107 AP2 (27-94) 1364 2 comp. including P7170 4603 Greater tol. to mannitol P9002 (RD29A (300 mM) prom.) G2107 AP2 (27-94) 1364 2 comp. including P7170 4603 Greater tol. to cold (8 C.) P9002 (RD29A prom.) G2108 AP2 (18-85) 1366 2 comp. including P4196 4543 Significantly greater P5297 (PG prom.) soluble solids (Brix) in tomato plants G2108 AP2 (18-85) 1366 2 comp. including P4196 4543 Significantly greater P5297 (PG prom.) lycopene in tomato plants G2109 MADS (1-57) 1368 Const. 35S prom. P2418 4381 Much less sensitive to ABA in a germination assay than wild-type G2110 WRKY 1370 Const. 35S prom. P2048 4329 Greater tol. to NaCl (239-298) (determined with 150 mM NaCl) G2110 WRKY 1370 Const. 35S prom. P2048 4329 More tol. to drought* (239-298) and better recovery from drought treatment* G2111 MADS (1-57) 1372 Const. 35S prom. P15002 4653 Altered sugar sensing response; decreased growth and small, pale seedlings on glucose medium G2113 AP2 (55-122) 1374 Const. 35S prom. P1699 4229 Altered light response; greater shade tol.; lack of shade avoidance phenotype; vertically oriented leaves with long petioles, elongated hypocotyls G2114 AP2 (221-295, 1376 Const. 35S prom. P1697 4228 Inc. seed size 323-393) G2116 bZIP (150-210) 1380 2 comp. including P4605 4582 Significantly greater P5297 (PG prom.) lycopene in tomato plants G2117 bZIP (46-106) 1382 Const. 35S prom. P1939 4285 Inc. seed protein content G2117 bZIP (46-106) 1382 Const. 35S prom. P1939 4285 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G2123 GF14 (99-109) 1384 Const. 35S prom. P1767 4253 More seed oil content G2124 TEO (75-132) 1386 Const. 35S prom. P1625 4211 Narrow curled leaves with elongated petioles G2129 bZIP (71-140) 1388 Const. 35S prom. P2472 4401 Early flowering G2130 AP2 (101-169) 1390 Const. 35S prom. P1508 4170 Better germination in heat (32 C.) G2131 AP2 (50-121, 1392 Const. 35S prom. P1835 4269 Altered C/N sensing: 146-217) greater tol. to low nitrogen conditions in C/N sensing assay G2132 AP2 (84-151) 1394 2 comp. including P4229 4544 Significantly greater P5297 (PG prom.) soluble solids (Brix) in tomato plants G2136 MADS (43-100) 1396 Const. 35S prom. P2046 4328 Decreased leaf 18:3 fatty acids G2137 WRKY 1398 2 comp. including P4743 4586 Significantly greater (109-168) P5318 (STM soluble solids (Brix) in prom.) tomato plants G2138 AP2 (76-148) 1400 Const. 35S prom. P1577 4194 More seed oil content G2140 HLH/MYC 1402 Const. 35S prom. P2062 4335 Less sens. to ABA (170-227) G2140 HLH/MYC 1402 Const. 35S prom. P2062 4335 Inc. tol. to (170-227) hyperosmotic stress; better germination on 150 mM NaCl or 9.4% sucrose G2140 HLH/MYC 1402 Const. 35S prom. P2062 4335 More tol. to drought* (170-227) G2141 HLH/MYC 1404 2 comp. including P4753 4587 Significantly greater (306-364) P5297 (PG prom.) soluble solids (Brix) in tomato plants G2141 HLH/MYC 1404 2 comp. including P4753 4587 Significantly greater (306-364) P5297 (PG prom.) lycopene in tomato plants G2142 HLH/MYC 1406 Const. 35S prom. P2444 4393 More tolerant to (42-100) phosphate deprivation in a root growth assay G2142 HLH/MYC 1406 Const. 35S prom. P2444 4393 Early flowering (42-100) G2143 HLH/MYC 1408 Const. 35S prom. P1905 4280 Altered inflorescence (122-179) development G2143 HLH/MYC 1408 Const. 35S prom. P1905 4280 Altered leaf shape, (122-179) darker green color G2143 HLH/MYC 1408 Const. 35S prom. P1905 4280 Altered flower (122-179) development, ectopic carpel tissue G2144 HLH/MYC 1410 Const. 35S prom. P1906 4281 Early flowering (207-265) G2144 HLH/MYC 1410 Const. 35S prom. P1906 4281 Pale green leaves, (207-265) altered leaf shape G2144 HLH/MYC 1410 Const. 35S prom. P1906 4281 Altered light response; (207-265) greater shade tol.; lack of shade avoidance phenotype; long cotyledons, long hypocotyls, pale, narrow, flat leaves that had long petioles G2144 HLH/MYC 1410 Const. 35S prom. P1906 4281 Altered C/N sensing: (207-265) greater tol. to low nitrogen conditions in C/N sensing assay G2145 HLH/MYC 1412 Const. 35S prom. P2064 4336 Altered C/N sensing: (170-227) greater tol. to low nitrogen conditions in C/N sensing assay G2145 HLH/MYC 1412 2 comp. including P4754 4588 Significantly greater (170-227) P5284 (RBCS3 lycopene in tomato prom.) plants G2146 HLH/MYC 1414 Const. 35S prom. P2386 4366 Insensitive to ABA (132-189) G2146 HLH/MYC 1414 Const. 35S prom. P2386 4366 More branching, short (132-189) internodes, inflorescences were shorter and bushier than wild type G2146 HLH/MYC 1414 Const. 35S prom. P2386 4366 Darker green (132-189) appearance G2146 HLH/MYC 1414 Const. 35S prom. P2386 4366 Late flowering (132-189) G2147 HLH/MYC 1416 Const. 35S prom. P1840 4271 Inc. leaf 16:0 fatty (163-220) acids and inc. leaf 18:2 fatty acids T2 lines G2150 HLH/MYC 1418 2 comp. including P4598 4579 Significantly greater (194-252) P5287 (LTP1 soluble solids (Brix) in prom.) tomato plants G2184 NAC (17-147) 1428 Const. 35S prom. P2623 4435 Early flowering G2192 bZIP-NIN 1430 Const. 35S prom. P1944 4286 Altered seed fatty acid (600-700) composition G2207 bZIP-NIN 1432 Const. 35S prom. P2490 4406 More tol. to (180-227, hyperosmotic stress; 546-627) better germination on 150 mM NaCl or 9.4% sucrose G2207 bZIP-NIN 1432 Const. 35S prom. P2490 4406 Less sensitive to ABA (180-227, 546-627) G2207 bZIP-NIN 1432 Const. 35S prom. P2490 4406 Narrow darker green (180-227, leaves 546-627) G2207 bZIP-NIN 1432 Const. 35S prom. P2490 4406 Late flowering (180-227, 546-627) G2213 bZIP-NIN 1434 Const. 35S prom. P2475 4403 Lethal when (156-205) constitutively overexpressed G2215 bZIP-NIN 1436 Const. 35S prom. P1948 4287 Altered light response; (150-246) greater shade tol.; lack of shade avoidance phenotype G2226 RING/C3H2C3 1438 Const. 35S prom. P15030 4662 Altered inflorescence (103-144) architecture; inflorescences had reduced internode elongation and short bushy overall stature, fertility reduced G2226 RING/C3H2C3 1438 Const. 35S prom. P15030 4662 Smaller plants (103-144) G2226 RING/C3H2C3 1438 Const. 35S prom. P15030 4662 Small darker green (103-144) rounded leaves G2227 RING/C3H2C3 1440 Const. 35S prom. P15063 4671 Smaller plants (199-239) G2227 RING/C3H2C3 1440 Const. 35S prom. P15063 4671 Narrow, curled, twisted (199-239) leaves G2239 RING/C3H2C3 1442 Const. 35S prom. P15351 4696 Altered C/N sensing: (128-169) greater tol. to low nitrogen conditions in C/N sensing assay G2251 RING/C3H2C3 1444 Const. 35S prom. P15069 4672 Reduced plant size (89-132) G2251 RING/C3H2C3 1444 Const. 35S prom. P15069 4672 Round and darker (89-132) green leaves G2251 RING/C3H2C3 1444 Const. 35S prom. P15069 4672 Short inflorescence (89-132) internodes G2251 RING/C3H2C3 1444 Const. 35S prom. P15069 4672 Late flowering (89-132) G2269 RING/C3H2C3 1446 Const. 35S prom. P15073 4674 Late flowering (136-177) G2290 WRKY 1448 Const. 35S prom. P2043 4327 Dwarfing and reduced (147-205) lignin in stem based on phloroglucinol stain (lignin was absent from intervasicular regions) G2291 AP2 (113-180) 1450 Const. 35S prom. P1692 4226 Altered inflorescence: decreased apical dominance, some secondary shoots grew to the same length as the primary shoot G2295 MADS (1-57) 1454 Const. 35S prom. P2042 4326 Early flowering G2295 MADS (1-57) 1454 Const. 35S prom. P2042 4326 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G2296 WRKY (85-145) 1456 2 comp. including P4741 4585 Significantly greater P5324 (Cru prom.) soluble solids (Brix) in tomato plants G2298 AP2 (4-70) 1458 Const. 35S prom. P2052 4331 Lethal when constitutively overexpressed G2311 MYB- 1460 Const. 35S prom. P2789 4468 Early flowering related (6-54) G2313 MYB- 1462 2 comp. including P4382 4553 Significantly greater related (111-159) P5319 (AS1 lycopene in tomato prom.) plants G2317 MYB- 1464 Const. 35S prom. P15033 4663 More tol. to NaCl (150 mM); related (51-97) enhanced root growth and greener than wild-type on NaCl (150 mM) G2317 MYB- 1464 Const. 35S prom. P15033 4663 More tol. to cold (8 C.) related (51-97) G2319 MYB- 1466 Const. 35S prom. P13388, 46,184,629 Greater tol. to NaCl related (44-90) P13446 (determined with 150 mM NaCl) G2319 MYB- 1466 Const. 35S prom. P13388, 46,184,629 Late flowering related (44-90) P13446 G2334 GRF-like 1468 Const. 35S prom. P15569 4725 Large leaves with (82-194) considerably more vegetative biomass G2334 GRF-like 1468 Const. 35S prom. P15569 4725 Late flowering (82-194) G2334 GRF-like 1468 Const. 35S prom. P15569 4725 Darker green leaves (82-194) G2340 MYB- 1470 Const. 35S prom. P1620 4210 Altered seed (R1)R2R3 glucosinolate profile (14-120) G2343 MYB- 1472 Const. 35S prom. P1610 4205 More seed oil content (R1)R2R3 (14-116) G2346 SBP (59-135) 1478 Const. 35S prom. P2025 4318 Enlarged seedling size G2347 SBP (60-136) 1480 Const. 35S prom. P1618 4208 Early flowering G2348 SBP (123-218) 1482 Const. 35S prom. P1617 4207 Early flowering G2371 ABI3/VP-1 1484 Const. 35S prom. P1856 4273 Darker green leaves (25-127) G2371 ABI3/VP-1 1484 Const. 35S prom. P1856 4273 Pale seed coloration (25-127) G2372 ARF (18-378) 1486 Const. 35S prom. P2596 4427 Reduced plant size G2372 ARF (18-378) 1486 Const. 35S prom. P2596 4427 Darker green leaves G2372 ARF (18-378) 1486 Const. 35S prom. P2596 4427 Early flowering G2372 ARF (18-378) 1486 Const. 35S prom. P2596 4427 Altered inflorescence determinacy and reduced fertility; inflorescences poorly developed and yielded few siliques, many of the lines exhibited terminal flowers G2373 TH (290-350) 1488 Const. 35S prom. P1949 4288 Early flowering G2375 TH (51-148) 1490 Const. 35S prom. P2833 4484 Small, narrow leaves G2375 TH (51-148) 1490 Const. 35S prom. P2833 4484 Smaller plants G2379 TH (19-110, 1492 Const. 35S prom. P1951 4289 Altered sugar sensing; 173-232) greater tol. to sucrose (determined in 9.4% sucrose) G2382 TH (90-177, 1494 Const. 35S prom. P2176 4358 Less sens. to ABA 246-333) G2383 TEO (89-149) 1496 Const. 35S prom. P1724 4240 Early senescence G2394 RING/C3H2C3 1498 Const. 35S prom. P15077 4675 Inc. tol. to NaCl (355-395) (determined with 150 mM NaCl) G2404 RING/C3H2C3 1500 Const. 35S prom. P15354 4697 Inc. tol. to NaCl (319-359) (determined with 150 mM NaCl) G2417 GARP (235-285) 1502 2 comp. including P4394 4557 Significantly greater P5287 (LTP1 lycopene in tomato prom.) plants G2423 MYB- 1504 2 comp. including P8576 4611 Inc. res. to Botrytis (R1)R2R3 P5486 (35S prom, (20-122) 35S::oEnh::LexA GaL4::TA::GR.) G2425 MYB- 1506 2 comp. including P4396 4558 Significantly greater (R1)R2R3 P5287 (LTP1 soluble solids (Brix) in (12-119) prom.) tomato plants G2425 MYB- 1506 2 comp. including P4396 4558 Significantly greater (R1)R2R3 P5326 (AP1 tomato plant volume (12-119) prom.) G2425 MYB- 1506 2 comp. including P4396 4558 Significantly greater (R1)R2R3 P5303 (PD prom.) tomato plant volume (12-119) G2425 MYB- 1506 2 comp. including P4396 4558 Significantly greater (R1)R2R3 P5284 (RBCS3 soluble solids (Brix) in (12-119) prom.) tomato plants G2430 GARP (425-478) 1508 Const. 35S prom. P1857 4274 More tol. to heat (32 C.) G2430 GARP (425-478) 1508 Const. 35S prom. P1857 4274 Greater leaf size, faster development G2432 Z-Dof (64-106) 1510 Const. 35S prom. P2502 4409 Shade tolerant phenotype; narrow, upward pointing leaves G2432 Z-Dof (64-106) 1510 Const. 35S prom. P2502 4409 Infertile flowers G2432 Z-Dof (64-106) 1510 Const. 35S prom. P2502 4409 Late flowering G2436 Z-CO-like 1512 Const. 35S prom. P2076 4342 Late flowering (16-111) G2443 Z-CO-like 1514 Const. 35S prom. P3320 4497 Early flowering (20-86) G2452 MYB- 1516 Const. 35S prom. P2023 4316 Pale; altered light related (28-79, response; greater shade 146-194) tol.; lack of shade avoidance phenotype G2452 MYB- 1516 Const. 35S prom. P2023 4316 More secondary rosette related (28-79, leaves, more secondary 146-194) shoots G2453 YABBY 1518 Const. 35S prom. P2750, 44,614,498 Better recovery from (52-91, 161-207) P3322 drought treatment* G2453 YABBY 1518 Const. 35S prom. P2750, 44,614,498 Greater pigment (52-91, 161-207) P3322 production G2453 YABBY 1518 Const. 35S prom. P2750, 44,614,498 Inc. tol. to NaCl (52-91, 161-207) P3322 (determined with 150 mM NaCl) G2453 YABBY 1518 Const. 35S prom. P2750, 44,614,498 Darker green curled (52-91, 161-207) P3322 leaves G2453 YABBY 1518 Const. 35S prom. P2750, 44,614,498 Smaller plants (52-91, 161-207) P3322 G2455 YABBY 1520 Const. 35S prom. P2584 4425 Altered light response; (10-48, 107-154) greater shade tol.; lack of shade avoidance phenotype G2455 YABBY 1520 Const. 35S prom. P2584 4425 Narrow, downward (10-48, 107-154) curled leaves G2456 YABBY 1522 Const. 35S prom. P2752 4462 Curled and darker (25-63, 148-195) green leaves G2456 YABBY 1522 Const. 35S prom. P2752 4462 Greater pigment (25-63, 148-195) production G2456 YABBY 1522 Const. 35S prom. P2752 4462 Smaller plants (25-63, 148-195) G2457 YABBY 1524 Const. 35S prom. P15094 4677 Multiple flower (21-59, 110-157) alterations; floral internodes short and floral organs narrow or absent, tiny contorted siliques that yielded few seeds G2457 YABBY 1524 Const. 35S prom. P15094 4677 Altered leaf shape; (21-59, 110-157) narrow, curled leaves G2457 YABBY 1524 Const. 35S prom. P15094 4677 Inc. tol. to NaCl (21-59, 110-157) (determined with 150 mM NaCl) G2459 YABBY 1526 Const. 35S prom. P15446 4703 Smaller plants (11-49, 100-147) G2459 YABBY 1526 Const. 35S prom. P15446 4703 Curled leaves (11-49, 100-147) G2459 YABBY 1526 Const. 35S prom. P15446 4703 Greater pigment (11-49, 100-147) production G2465 GARP (219-269) 1528 Const. 35S prom. P1858 4275 Later bolting, later flowering and later senescing G2467 HS (28-119) 1530 Const. 35S prom. P2744 4460 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2467 HS (28-119) 1530 Const. 35S prom. P2744 4460 Early senescence G2492 ENBP (197-211, 1532 Const. 35S prom. P13700 4632 Smaller plants 616-860) G2505 NAC (9-137) 1534 Const. 35S prom. P1533 4178 Altered light response; greater shade tol.; lack of shade avoidance phenotype, when grown under white light G2505 NAC (9-137) 1534 Const. 35S prom. P1533 4178 Inc. tol. to drought* G2505 NAC (9-137) 1534 2 comp. including P4342 4547 Significantly greater P5284 (RBCS3 lycopene in tomato prom.) plants G2509 AP2 (89-156) 1536 Const. 35S prom. P2039 4325 Decreased seed oil content G2509 AP2 (89-156) 1536 Const. 35S prom. P2039 4325 Inc. seed protein content G2509 AP2 (89-156) 1536 Const. 35S prom. P2039 4325 Altered seed prenyl lipids; inc. in seed alpha-tocopherol G2509 AP2 (89-156) 1536 Const. 35S prom. P2039 4325 Reduced apical dominance G2509 AP2 (89-156) 1536 Const. 35S prom. P2039 4325 Early flowering G2510 AP2 (42-109) 1538 Const. 35S prom. P2038 4324 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 Late flowering P6506 (35S prom.) G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 Darker green, glossy P6506 (35S prom.) leaves G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 Inc. tol. to cold (8 C.) P6506 (35S prom.) G2513 AP2 (27-94) 1542 Const. 35S prom. P1830 4266 More tol. to heat (32 C.) G2513 AP2 (27-94) 1542 Const. 35S prom. P1830 4266 More tol. to heat (32 C.) G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 More tol. to NaCl (150 mM) P9002 (RD29A prom.) G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 Late flowering P9002 (RD29A prom.) G2513 AP2 (27-94) 1542 2 comp. including P4566 4577 Glossy leaves P9002 (RD29A prom.) G2513 AP2 (27-94) 1542 Knockout not Late flowering applicable G2513 AP2 (27-94) 1542 Knockout not Inc. tol. to cold (8 C.) applicable G2513 AP2 (27-94) 1542 Knockout not Darker green leaves applicable G2515 MADS (1-57) 1546 Const. 35S prom. P13372 4615 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2515 MADS (1-57) 1546 Const. 35S prom. P13372 4615 Early flowering G2515 MADS (1-57) 1546 Const. 35S prom. P13372 4615 Altered inflorescence determinacy; flowers exhibited numerous non-specific abnormalities, an occasional line displayed terminal flowers G2515 MADS (1-57) 1546 Const. 35S prom. P13372 4615 Altered flower morphology; small inflorescences G2515 MADS (1-57) 1546 Const. 35S prom. P13372 4615 Smaller plant G2520 HLH/MYC 1550 Const. 35S prom. P2066 4337 Altered seed prenyl (139-197) lipids; altered tocopherol composition G2520 HLH/MYC 1550 Const. 35S prom. P2066 4337 Altered C/N sensing: (139-197) much greater tol. to low nitrogen conditions in C/N sensing assay G2520 HLH/MYC 1550 Const. 35S prom. P2066 4337 Altered light response; (139-197) greater shade tol.; lack of shade avoidance phenotype; curled cotyledons, long hypocotyls, slightly pale inflorescences G2522 AT-hook 1552 Const. 35S prom. P1743 4247 Altered C/N sensing: (101-109, greater tol. to low 196-240) nitrogen conditions in C/N sensing assay G2525 DBP (196-308) 1554 Const. 35S prom. P15096 4678 Inc. sens. to cold (8 C.) G2531 NAC (52-212) 1556 Const. 35S prom. P2030 4322 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G2535 NAC (11-114) 1558 Const. 35S prom. P1735 4244 Altered C/N sensing: accumulated more anthocyanin in C/N sensing assay G2536 NAC (5-135) 1560 Const. 35S prom. P15377 4701 Larger leaf size G2536 NAC (5-135) 1560 Const. 35S prom. P15377 4701 Inc. biomass; greater plant size G2536 NAC (5-135) 1560 Const. 35S prom. P15377 4701 Delayed senescence G2543 HB (31-91) 1564 Const. 35S prom. P1897 4277 Inc. sens. to cold (8 C.) G2550 HB (345-408) 1566 Const. 35S prom. P16180 4740 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2550 HB (345-408) 1566 Const. 35S prom. P16180 4740 Darker green curled, narrow leaves G2550 HB (345-408) 1566 Const. 35S prom. P16180 4740 Altered inflorescence architecture; short, compact, bushy inflorescences, reduced internode elongation, flowers bunched together at the tips G2552 HLH/MYC 1568 Const. 35S prom. P2068 4338 Increase leaf (124-181) glucosinolate M39480 G2557 HLH/MYC 1572 Const. 35S prom. P1993 4303 Altered leaf shape, (272-329) darker green color G2557 HLH/MYC 1572 Const. 35S prom. P1993 4303 Altered flower (272-329) development, ectopic carpel tissue G2559 DBP (60-170) 1574 Const. 35S prom. P15538 4722 Late flowering G2565 GARP (243-292) 1576 Const. 35S prom. P15481 4710 Reduced plant size G2565 GARP (243-292) 1576 Const. 35S prom. P15481 4710 Grayish leaf coloration and altered leaf shape; in some lines leaves were broad and flat, whereas in other lines they were pointed, narrow and curled G2567 ARF (18-384) 1578 Const. 35S prom. P2597 4428 More tol. to cold (8 C.) G2570 GARP (235-283) 1580 Const. 35S prom. P15454 4705 Lethal when constitutively overexpressed G2571 AP2 (133-200) 1582 Const. 35S prom. P1998 4304 Altered light response; greater shade tol.; lack of shade avoidance phenotype G2571 AP2 (133-200) 1582 Const. 35S prom. P1998 4304 Altered coloration G2571 AP2 (133-200) 1582 Const. 35S prom. P1998 4304 Altered branching patterns, disorganized rosette phyllotaxy and stunted shoot outgrowth G2571 AP2 (133-200) 1582 Const. 35S prom. P1998 4304 Altered leaf and flower development; twisted rosette leaves and short stems, many flowers with abnormal organs, sympodial in the inflorescence G2574 WRKY 1584 Const. 35S prom. P2414 4379 Premature leaf (225-284) senescence G2574 WRKY 1584 Const. 35S prom. P2414 4379 Smaller plants (225-284) G2575 WRKY 1586 Const. 35S prom. P15034 4664 Altered leaf shape; (137-192) narrow cotyledons and leaves and leaf serrations G2575 WRKY 1586 Const. 35S prom. P15034 4664 Altered inflorescence (137-192) architecture; short floral internodes and relatively few seeds G2579 AP2 (52-119) 1588 Const. 35S prom. P15040 4666 Altered silique size and shape; flat stumpy club-like siliques G2579 AP2 (52-119) 1588 Const. 35S prom. P15040 4666 Increased carpel size and infertile; wide carpels G2579 AP2 (52-119) 1588 Const. 35S prom. P15040 4666 Smaller plants G2579 AP2 (52-119) 1588 Const. 35S prom. P15040 4666 Altered leaf shape; narrow curled leaves with short petioles G2579 AP2 (52-119) 1588 Const. 35S prom. P15040 4666 Inc. tol. to cold (8 C.) G2585 WRKY 1592 Const. 35S prom. P2412 4378 Larger seed (103-162) G2587 WRKY 1594 Const. 35S prom. P2096 4346 Lethal when (108-165) constitutively overexpressed G2589 MADS (1-57) 1596 Const. 35S prom. P2004 4306 Darker green G2590 MADS (2-57) 1598 Const. 35S prom. P2005 4307 Altered C/N sensing: greater tol. to low nitrogen conditions in C/N sensing assay G2592 TUBBY 1600 Const. 35S prom. P2910 4485 Inc. sens. to cold (8 C.) (119-429) G2603 TUBBY 1602 Const. 35S prom. P2438 4391 Inc. tol. to cold; (104-389) seedlings are larger and greener when germinated at 8 C. G2603 TUBBY 1602 Const. 35S prom. P2438 4391 Late developing (104-389) G2604 Z-LSDlike 1604 Const. 35S prom. P2644 4440 Altered C/N sensing: (34-64, 73-103) greater tol. to low nitrogen conditions in C/N sensing assay G2604 Z-LSDlike 1604 Const. 35S prom. P2644 4440 Late flowering (34-64, 73-103) G2604 Z-LSDlike 1604 Const. 35S prom. P2644 4440 Altered leaf surface, (34-64, 73-103) gray leaves G2616 HB (79-139) 1606 Const. 35S prom. P15472 4708 Smaller plants G2616 HB (79-139) 1606 Const. 35S prom. P15472 4708 Altered inflorescence architecture and flower development; inflorescences were short and bushy, some flowers replaced by sterile filamentous structures G2617 Z-C2H2 1608 Const. 35S prom. P2806 4476 Less sens. to ABA (57-77) G2617 Z-C2H2 1608 Const. 35S prom. P2806 4476 Faster growth rate for (57-77) seedlings and early stage plants G2617 Z-C2H2 1608 Const. 35S prom. P2806 4476 Short petioles, short (57-77) pedicels G2617 Z-C2H2 1608 Const. 35S prom. P2806 4476 Wrinkled, curled, (57-77) rounded leaves G2628 bZIP (36-105) 1610 Const. 35S prom. P2483 4405 Early flowering G2628 bZIP (36-105) 1610 Const. 35S prom. P2483 4405 Rounded leaves G2628 bZIP (36-105) 1610 Const. 35S prom. P2483 4405 Smaller plants G2633 SCR (197-262, 1616 Const. 35S prom. P2381 4364 Early flowering 325-413, 417-489) G2636 NAC (14-146) 1618 Const. 35S prom. P2793 4470 Altered morphology; alterations in rosette leaf initiation by the shoot meristem; lobed leaves; adventitious shoots on the adaxial surface of lobed cotyledons G2639 SRS (114-167) 1620 Const. 35S prom. P15568 4724 Short inflorescence internodes G2639 SRS (114-167) 1620 Const. 35S prom. P15568 4724 Early flowering G2639 SRS (114-167) 1620 Const. 35S prom. P15568 4724 Altered flower morphology and poorly fertile; inflorescences were bushy and carried flowers that displayed a variety of non- specific defects G2640 SRS (146-189) 1622 Const. 35S prom. P2675 4448 Altered light response; greater shade tol.; lack of shade avoidance phenotype; seedlings were slightly larger than controls under white light G2640 SRS (146-189) 1622 Const. 35S prom. P2675 4448 Altered flower morphology and poor fertility; organs often poorly developed G2640 SRS (146-189) 1622 Const. 35S prom. P2675 4448 Smaller plants G2640 SRS (146-189) 1622 Const. 35S prom. P2675 4448 Darker green leaves with glossy surfaces G2640 SRS (146-189) 1622 Const. 35S prom. P2675 4448 Short inflorescence internodes G2649 SRS (112-155) 1624 Const. 35S prom. P15495 4714 Short inflorescence internodes G2649 SRS (112-155) 1624 Const. 35S prom. P15495 4714 Darker green, glossy leaf surface and elongated leaf shape G2649 SRS (112-155) 1624 Const. 35S prom. P15495 4714 Altered flower morphology; inflorescences were short, bushy, flowers were poorly fertile, Siliques very narrow, curled and yielded relatively few seeds G2649 SRS (112-155) 1624 Const. 35S prom. P15495 4714 Smaller plants G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 T2 plants developed excessive numbers of small axillary rosette leaves G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 Shade tolerant phenotype; long narrow leaves; elongated petioles; long hypocotyls; leaves were held in a more upright orientation than controls G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 Inc. size; larger seedlings and mature plants G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 More tol. to cold (8 C.) G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 Inc. number of axillary meristems in the rosettes G2650 TEO (34-91) 1626 Const. 35S prom. P2603 4430 Early flowering G2655 HLH/MYC 1628 Const. 35S prom. P2452 4394 Poorly developed and (119-178) greenish roots G2661 HLH/MYC 1630 Const. 35S prom. P2454 4395 Altered sugar sensing; (40-97) more tol. to 5% glucose G2661 HLH/MYC 1630 Const. 35S prom. P2454 4395 Darker plants (40-97) G2679 CPP (107-177) 1632 Const. 35S prom. P15056 4670 Enhanced seedling vigor G2682 CPP (67-181) 1634 Const. 35S prom. P15043 4667 Curled leaves G2682 CPP (67-181) 1634 Const. 35S prom. P15043 4667 Smaller plants G2686 WRKY 1636 Const. 35S prom. P2095 4345 Altered light response; (122-173) greater shade tol.; lack of shade avoidance phenotype G2686 WRKY 1636 Const. 35S prom. P2095 4345 Rounded leaves with (122-173) slightly lobed margins G2690 AP2 (46-108, 1638 Const. 35S prom. P2093 4344 Narrow, darker green 176-275) leaves that roll down at the margins G2691 AP2 (78-146) 1640 Const. 35S prom. P2408 4376 Greater tol. to NaCl (determined with 150 mM NaCl) G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Late flowering G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Greater seedling size G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Altered inflorescence architecture; some inflorescences had a very leafy appearance; an inc. number of coflorescence nodes, and a higher order of branching G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Altered leaf shape, darker green leaves G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Multiple flower alterations; some sepals were enlarged and bract-like, petals and stamens were somewhat contorted, pollen production was low, and carpels were wider than in wild type G2694 OTHER (1-446) 1642 Const. 35S prom. P13429 4623 Altered light response; greater shade tol.; lack of shade avoidance phenotype; long petioles, narrow leaf blades, leaves held in a more vertical orientation G2699 SCR (107-172, 1644 Const. 35S prom. P3279 4486 Altered leaf shape; 243-333, long petioles and large 333-407) leaves G2701 MYB- 1646 Const. 35S prom. P2012 4310 More tol. to related (31-81, hyperosmotic stress; 127-175) better germination on 150 mM NaCl or 9.4% sucrose G2701 MYB- 1646 Const. 35S prom. P2012 4310 More tol. to drought* related (31-81, and better recovery 127-175) from drought treatment* G2702 MYB- 1648 Const. 35S prom. P13807 4644 Altered light response; (R1)R2R3 greater shade tol.; lack (31-131) of shade avoidance phenotype G2702 MYB- 1648 Const. 35S prom. P13807 4644 Smaller plants (R1)R2R3 (31-131) G2702 MYB- 1648 Const. 35S prom. P13807 4644 Dark, round leaves (R1)R2R3 with short petioles (31-131) G2713 TUBBY 1650 Const. 35S prom. P1982 4299 More tol. to NaCl; (123-445) seedlings have longer roots in 150 mM NaCl G2717 MYB- 1652 Const. 35S prom. P2796 4472 More tol. to related (6-54) hyperosmotic stress; better germination on 150 mM NaCl or 9.4% sucrose G2717 MYB- 1652 Const. 35S prom. P2796 4472 More tol. to drought* related (6-54) G2719 MYB- 1656 Const. 35S prom. P2009 4308 Altered sugar sensing; (R1)R2R3 greater tol. to sucrose (56-154) (determined in 9.4% sucrose) G2719 MYB- 1656 Const. 35S prom. P2009 4308 Altered C/N sensing: (R1)R2R3 much greater tol. to (56-154) low nitrogen conditions in C/N sensing assay G2723 MYB- 1658 Const. 35S prom. P2770 4464 Late flowering related (10-60) G2724 MYB- 1660 Const. 35S prom. P2014 4311 Darker green leaves (R1)R2R3 (7-113) G2741 GARP (149-197) 1662 Const. 35S prom. P2384 4365 Late flowering G2741 GARP (149-197) 1662 Const. 35S prom. P2384 4365 Inc. biomass; larger plants at late stages of development G2743 GARP (201-249) 1664 Const. 35S prom. P2390 4369 Late flowering G2743 GARP (201-249) 1664 Const. 35S prom. P2390 4369 Altered flower development; sepals, petals and stamens were reduced in size, pollen production was poor G2747 ABI3/VP-1 1666 Const. 35S prom. P2470 4400 Long petioles and (19-113) slightly narrow elongated leaf blades, little or no secondary root formation G2754 SWI/SNF 1668 Const. 35S prom. P13851 4650 Altered light response; (198-393, greater shade tol.; lack 554-638) of shade avoidance phenotype; seedlings slightly pale in coloration, long hypocotyls, elongated petioles, and leaves held in a more upright orientation G2754 SWI/SNF 1668 Const. 35S prom. P13851 4650 Early flowering (198-393, 554-638) G2757 TH (35-123, 1670 Const. 35S prom. P13712 4634 Smaller plants 348-434) G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 Altered light response; (141-201) greater shade tol.; lack of shade avoidance phenotype G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 Late flowering (141-201) G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 More sens. to cold (8 C.) (141-201) G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 More sens. to 5% (141-201) glucose G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 More anthocyanin (141-201) accumulation in seedlings G2763 HLH/MYC 1672 Const. 35S prom. P2387 4367 Darker green leaves (141-201) G2765 HLH/MYC 1674 Const. 35S prom. P2829 4482 Retarded growth at (128-185) early stages G2768 DBP (288-346) 1678 Const. 35S prom. P15431 4702 Greater leaf size G2768 DBP (288-346) 1678 Const. 35S prom. P15431 4702 Greater petal number, loss of floral determinacy G2771 HLH/MYC 1680 Const. 35S prom. P15182 4686 Altered leaf; narrow, (345-402) long and curled and darker green leaves G2771 HLH/MYC 1680 Const. 35S prom. P15182 4686 More tol. to cold (8 C.) (345-402) G2771 HLH/MYC 1680 Const. 35S prom. P15182 4686 Late flowering (345-402) G2771 HLH/MYC 1680 Const. 35S prom. P15182 4686 Altered light response; (345-402) greater shade tol.; lack of shade avoidance phenotype; elongated hypocotyl and pale in coloration G2774 HLH/MYC 1682 Const. 35S prom. P16177 4738 Altered light response; (158-215) greater shade tol.; lack of shade avoidance phenotype G2776 HLH/MYC 1684 Const. 35S prom. P2456 4396 Altered sugar sensing; (145-202) seedlings on 9.4% sucrose were larger with green cotyledons G2776 HLH/MYC 1684 Const. 35S prom. P2456 4396 More tol. to drought* (145-202) G2777 HLH/MYC 1686 Const. 35S prom. P2559 4423 Early flowering (273-331) G2779 HLH/MYC 1688 Const. 35S prom. P15228 4688 Pale leaves (148-206) G2779 HLH/MYC 1688 Const. 35S prom. P15228 4688 Early flowering (148-206) G2783 ACBF-like 1690 Const. 35S prom. P2554 4421 Early senescence (63-124, 151-235, 262-318) G2783 ACBF-like 1690 Const. 35S prom. P2554 4421 Smaller plants (63-124, 151-235, 262-318) G2784 DBP (139-260) 1692 Const. 35S prom. P15148 4681 Altered inflorescence architecture; secondary shoots that grew downwards G2784 DBP (139-260) 1692 Const. 35S prom. P15148 4681 Slow growth rate G2784 DBP (139-260) 1692 Const. 35S prom. P15148 4681 Darker green and curled leaves G2784 DBP (139-260) 1692 Const. 35S prom. P15148 4681 Inc. tol. to cold (8 C.) G2789 AT-hook 1694 Const. 35S prom. P2058 4334 Altered sugar sensing; (59-67, 67-208) greater tol. to sucrose (determined in 9.4% sucrose) G2789 AT-hook 1694 Const. 35S prom. P2058 4334 More tol. to drought* (59-67, 67-208) and better recovery from drought treatment* G2789 AT-hook 1694 Const. 35S prom. P2058 4334 Less sens. to ABA (59-67, 67-208) G2789 AT-hook 1694 Const. 35S prom. P2058 4334 Altered light response; (59-67, 67-208) greater shade tol.; lack of shade avoidance phenotype G2789 AT-hook 1694 Const. 35S prom. P2058 4334 Altered C/N sensing: (59-67, 67-208) much greater tol. to low nitrogen conditions in C/N sensing assay G2790 HLH/MYC 1696 Const. 35S prom. P2395 4372 More sens. to cold (8 C.) (141-198) G2802 NAC (48-196) 1700 Const. 35S prom. P2771 4465 With P2771 (with antisense construct); altered flowering time; transformants harboring antisense clone exhibited early flowering, transformants harboring a sense clone exhibited late flowering G2802 NAC (48-196) 1700 Const. 35S prom. P15486 4712 With P15486 (sense construct); altered flowering time; transformants harboring antisense clone exhibited early flowering, transformants harboring a sense clone exhibited late flowering G2805 NAC (2-169) 1702 Const. 35S prom. P2773 4466 Early flowering G2826 Z-C2H2 1704 Const. 35S prom. P2757 4463 Aerial rosettes at (75-95) coflorescence nodes, indicating a disruption in phase change in the inflorescence G2826 Z-C2H2 1704 Const. 35S prom. P2757 4463 Ectopic trichome (75-95) formation; flowers had inc. trichome density on sepals and possessed ectopic trichomes on the carpels G2830 Z-C2H2 1706 Const. 35S prom. P3286 4487 Altered sugar sensing; (245-266) greater tol. to sucrose (determined in 9.4% sucrose) G2830 Z-C2H2 1706 Knockout not More seed oil content (245-266) applicable G2832 Z-C2H2 1708 Const. 35S prom. P2668 4447 Early flowering (11-31, 66-86, 317-337) G2832 Z-C2H2 1708 Const. 35S prom. P2668 4447 Pale gray leaf color (11-31, 66-86, 317-337) G2834 Z-C2H2 1710 Const. 35S prom. P2805 4475 Slow growth rate (246-266, 335-356) G2837 Z-C2H2 1712 Const. 35S prom. P3288 4488 Altered leaf shape, (140-160) darker green leaves G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Late flowering (57-77) G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Altered size; greater (57-77) seedling size G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Altered leaves; aerial (57-77) rosettes G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Darker green leaves (57-77) G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Greater trichome (57-77) density G2838 Z-C2H2 1714 Const. 35S prom. P15184 4687 Multiple flower (57-77) alterations; in some lines flowers had shoot like characteristics, and sepals from some flowers had a bract- like appearance G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 Altered sugar sensing; (34-60, 85-113) greater tol. to sucrose (determined in 9.4% sucrose) G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 More tol. to drought* (34-60, 85-113) and better recovery from drought treatment* G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 Short petioles (34-60, 85-113) G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 Small, contorted leaves (34-60, 85-113) that were up-curled at margins G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 Altered inflorescence; (34-60, 85-113) poorly developed flowers with downward-pointing short pedicels G2839 Z-C2H2 1716 Const. 35S prom. P2831 4483 Smaller plants (34-60, 85-113) G2846 HLH/MYC 1718 Const. 35S prom. P2553 4420 Darker green, narrow (267-324) curled leaves G2846 HLH/MYC 1718 Const. 35S prom. P2553 4420 Late flowering (267-324) G2846 HLH/MYC 1718 Const. 35S prom. P2553 4420 Smaller plants (267-324) G2847 HLH/MYC 1720 Const. 35S prom. P15106 4679 Darker green leaves (206-263) G2847 HLH/MYC 1720 Const. 35S prom. P15106 4679 Smaller plants (206-263) G2850 HLH/MYC 1722 Const. 35S prom. P13433 4624 Curled, darker green (320-376) leaves G2851 HLH/MYC 1724 Const. 35S prom. P2457 4397 Small, darker green, (250-307) curled and wrinkled leaves G2851 HLH/MYC 1724 Const. 35S prom. P2457 4397 Slow growing (250-307) G2851 HLH/MYC 1724 Const. 35S prom. P2457 4397 Smaller plants (250-307) G2854 ACBF-like 1726 Const. 35S prom. P2558 4422 Less sensitive to ABA (110-250) G2854 ACBF-like 1726 Const. 35S prom. P2558 4422 Altered sugar sensing; (110-250) greater tol. to sucrose (determined in 9.4% sucrose) G2854 ACBF-like 1726 Const. 35S prom. P2558 4422 More tol. to drought* (110-250) and better recovery from drought treatment* G2859 HLH/MYC 1728 Const. 35S prom. P2546 4419 Altered leaf shape, flat (150-208) and mild serrations, and light green leaves G2859 HLH/MYC 1728 Const. 35S prom. P2546 4419 Inflorescence (150-208) architecture; inflorescences became increasingly proliferated and bushy as the plants aged, exhibited very thin stems, long narrow curled cauline leaves, and carried flowers that were rather small and had poorly developed organs G2859 HLH/MYC 1728 Const. 35S prom. P2546 4419 Altered light response; (150-208) greater shade tol.; lack of shade avoidance phenotype; long hypocotyls, cotyledons; light green plants G2865 HLH/MYC 1730 Const. 35S prom. P2541 4418 Less sens. to ABA (88-153) G2866 IAA (84-100, 1732 Const. 35S prom. P15600 4728 Curled leaves 139-168) G2869 ARF (26-409) 1734 Const. 35S prom. P15601 4729 Lethal when constitutively overexpressed G2884 GARP (228-276) 1736 Const. 35S prom. P15666 4735 Abnormal embryo development G2884 GARP (228-276) 1736 Const. 35S prom. P15666 4735 Smaller plants G2884 GARP (228-276) 1736 Const. 35S prom. P15666 4735 Multiple flower defects and low fertility; feeble inflorescences that gave rise to few poorly developed flowers and contorted siliques G2884 GARP (228-276) 1736 Const. 35S prom. P15666 4735 Altered light response; greater shade tol.; lack of shade avoidance phenotype; long hypocotyls G2885 GARP (196-243) 1738 Const. 35S prom. P2599 4429 Altered cell differentiation; callus- like outgrowths were seen on the stems from 2 lines G2885 GARP (196-243) 1738 Const. 35S prom. P2599 4429 Decreased tol. to cold (8 C.) G2887 NAC (4-180) 1740 Const. 35S prom. P2826 4481 Lethal when constitutively overexpressed G2888 Z-C2H2 1742 Const. 35S prom. P2656 4444 Altered light response; (41-61, 120-140) greater shade tol.; lack of shade avoidance phenotype G2888 Z-C2H2 1742 Const. 35S prom. P2656 4444 Dark narrow curled (41-61, 120-140) leaves G2893 MYB- 1744 Const. 35S prom. P2016 4312 Darker green plants (R1)R2R3 (19-120) G2893 MYB- 1744 Const. 35S prom. P2016 4312 Reduced fertility; floral (R1)R2R3 organs generally (19-120) underdeveloped; but two lines formed large flowers with inc. stamen and carpel number G2893 MYB- 1744 2 comp. including P4729 4584 Significantly greater (R1)R2R3 P5326 (AP1 lycopene in tomato (19-120) prom.) plants G2893 MYB- 1744 2 comp. including P4729 4584 Significantly greater (R1)R2R3 P5318 (STM tomato fruit weight (19-120) prom.) when expressed under the STM promoter G2898 HMG (59-131) 1746 Const. 35S prom. P2589 4426 Altered sugar sensing; seedlings were larger and had better germination in 5% glucose G2907 PCGL (12-120, 1748 Const. 35S prom. P15595 4727 Accelerated 854-923) senescence G2913 ARID (43-127) 1750 Const. 35S prom. P13392 4619 Altered C/N sensing: more tol. to low nitrogen conditions in C/N sensing assay G2930 HLH/MYC 1752 Const. 35S prom. P2519 4413 More tol. to cold (8 C.) (57-120) G2933 HLH/MYC 1754 Const. 35S prom. P2392 4371 Larger seeds (68-128) G2933 HLH/MYC 1754 Const. 35S prom. P2392 4371 More tol. to cold (8 C.) (68-128) G2934 HLH/MYC 1756 Const. 35S prom. P3327 4500 Smaller plants (39-99) G2958 IAA (88-104, 1758 Const. 35S prom. P15168 4685 Altered inflorescence 143-172) architecture; compact inflorescence stems in which internodes were short G2958 IAA (88-104, 1758 Const. 35S prom. P15168 4685 Altered light response; 143-172) greater shade tol.; lack of shade avoidance phenotype G2958 IAA (88-104, 1758 Const. 35S prom. P15168 4685 Altered leaf; darker 143-172) green curled leaves G2958 IAA (88-104, 1758 Const. 35S prom. P15168 4685 Smaller plants 143-172) G2964 Z-C3H (41-63, 1760 Const. 35S prom. P2808 4477 Late flowering 201-235) G2964 Z-C3H (41-63, 1760 Const. 35S prom. P2808 4477 Aerial rosettes 201-235) G2967 Z-C2H2 1762 Const. 35S prom. P2633 4436 Early flowering (66-88, 358-385) G2969 Z-C2H2 1764 Const. 35S prom. P2368 4360 Altered sugar sensing; (128-150) seedlings were larger, greener and had more root growth in 9.4% sucrose G2969 Z-C2H2 1764 Const. 35S prom. P2368 4360 Less sensitive to ABA (128-150) in germination assays G2969 Z-C2H2 1764 Const. 35S prom. P2368 4360 More tol. to drought* (128-150) G2972 Z-C2H2 (8-32, 1766 Const. 35S prom. P2635 4437 More tol. to low 129-149, phosphate conditions 277-294) G2979 E2F (192-211) 1768 Const. 35S prom. P15531 4721 Late flowering G2979 E2F (192-211) 1768 Const. 35S prom. P15531 4721 Greater biomass G2979 E2F (192-211) 1768 Const. 35S prom. P15531 4721 Greater flower organ size and number G2981 E2F (155-173) 1770 Const. 35S prom. P2702 4449 Altered C/N sensing: more tol. to low nitrogen conditions in C/N sensing assay G2982 E2F (107-124) 1772 Const. 35S prom. P2703 4450 More tol. to dehydration G2982 E2F (107-124) 1772 Const. 35S prom. P2703 4450 More tol. to drought* G2983 HB (88-148) 1774 Const. 35S prom. P13821 4646 Ectopic carpel formation G2983 HB (88-148) 1774 Const. 35S prom. P13821 4646 Altered cell proliferation G2983 HB (88-148) 1774 Const. 35S prom. P13821 4646 Altered growth pattern, proliferation and root hair density G2983 HB (88-148) 1774 Const. 35S prom. P13821 4646 Altered cell differentiation, trichome cell fate G3008 EIL (10-275) 1802 Const. 35S prom. P15232 4689 Inc. biomass; large leaf size G3017 HLH/MYC 1804 Const. 35S prom. P13799 4641 Smaller plants (136-193) G3021 HLH/MYC 1806 Const. 35S prom. P2520 4414 Late flowering (91-148) G3021 HLH/MYC 1806 Const. 35S prom. P2520 4414 Altered inflorescence (91-148) architecture; stunted inflorescences in which the floral internodes were narrow G3021 HLH/MYC 1806 Const. 35S prom. P2520 4414 Altered leaf; uneven (91-148) surface texture, darker green leaves G3032 GARP (285-333) 1808 Const. 35S prom. P15514 4718 Altered light response; greater shade tol.; lack of shade avoidance phenotype; altered leaf orientation; pale color, vertically oriented leaves G3032 GARP (285-333) 1808 Const. 35S prom. P15514 4718 Early flowering G3044 HLH/MYC 1810 Const. 35S prom. P2528 4415 Early flowering (226-284) G3044 HLH/MYC 1810 Const. 35S prom. P2528 4415 Long, narrow, pale (226-284) leaves, with mildly serrated margins at later stages of growth G3054 Z-C3H (77-96, 1812 Const. 35S prom. P2388 4368 Less sens. to ABA 149-168) G3055 Z-C3H (97-115, 1814 Const. 35S prom. P2820 4480 Less sens. to ABA 178-197, 266-287) G3059 Z-C3H 1816 Const. 35S prom. P2649 4442 Accelerated (219-287) senescence G3059 Z-C3H 1816 Const. 35S prom. P2649 4442 Curled, contorted, dark (219-287) leaves G3059 Z-C3H 1816 Const. 35S prom. P2649 4442 Altered inflorescence (219-287) architecture; stunted inflorescences that were hooked at their apices G3059 Z-C3H 1816 Const. 35S prom. P2649 4442 Altered cotyledon (219-287) shape; oval cotyledons G3059 Z-C3H 1816 Const. 35S prom. P2649 4442 Smaller plants (219-287) G3060 Z-C3H (42-61, 1818 Const. 35S prom. P2819 4479 Altered flowering time; 219-237) some lines flowered early, and others flowered late G3061 Z-C2H2 1820 Const. 35S prom. P2657 4445 Early flowering (73-90, 174-193) G3067 Z-C2H2 1822 Const. 35S prom. P2391 4370 Insensitive to ABA in (198-219) germination assays G3070 Z-C2H2 1824 Const. 35S prom. P15661 4734 Gray leaf coloration (129-150) G3076 bZIP-ZW2 1830 Const. 35S prom. P13423 4622 Inc. tol. to dehydration (70-100, 182-209) G3083 bZIP-ZW2 1832 Const. 35S prom. P2480 4404 Greater tol. to NaCl (75-105, (determined with 150 mM 188-215) NaCl) G3084 IAA (94-110, 1834 Const. 35S prom. P13746 4637 Downward curled and 148-177) twisted leaves G3091 PLATZ (34-131) 1838 Const. 35S prom. P15518 4719 Darker green leaves with altered leaf shape: mild serrations on leaf margins and uneven surface texture G3094 PLATZ (7-143) 1840 Const. 35S prom. P15479 4709 Serrated leaves and long petioles G3094 PLATZ (7-143) 1840 Const. 35S prom. P15479 4709 Altered flower morphology; narrow sepals and petals G3095 PLATZ (16-151) 1842 Const. 35S prom. P15520 4720 Narrow darker green leaves with serrations on the margins G3095 PLATZ (16-151) 1842 Const. 35S prom. P15520 4720 Slow growth rate G3111 RING/C3H2C3 1844 Const. 35S prom. P15071 4673 Narrow, downward (111-152) curled, darker green leaves G3111 RING/C3H2C3 1844 Const. 35S prom. P15071 4673 Late flowering (111-152) G3111 RING/C3H2C3 1844 Const. 35S prom. P15071 4673 Accelerated (111-152) senescence G3967 VAR (1-1174) 2242 Knockout not Better recovery from applicable drought treatment* G1073 AT-hook 18 G1073 and Double P448, 3936 and Greater biomass, and (63-71, 71-216) and G1274 transcription factor P15038 4665 additive effect relative G1274 and 20 overexpression; to either parent WRKY const. 35S prom. overexpressor line (110-166) G1073 AT-hook 18 G1073 and Double P448 and 3936 and Early flowering; and (63-71, 71-216) and G3086 transcription factor P15046 4668 G3086 OE overcomes G3086 and 1836 overexpression; delayed flowering HLH/MYC const. 35S prom. associated with G1073 (307-365) OE G481 CAAT (20-109) 10 G481 and Double P46 and 3811 and Early flowering; and and and G3086 transcription factor P15046 4668 G3086 OE overcomes G3086 HLH/MYC 1836 overexpression; delayed flowering (307-365) const. 35S prom. associated with G481 OE G481 CAAT (20-109) 10 G481 and Double P46 and 3811 and Greater seedling vigor; and and and G1274 transcription factor P15038 4665 novel phenotype not G1274 WRKY 20 overexpression; typically seen in either (110-166) const. 35S prom. single parental overexpressor line G481 CAAT (20-109 10 G481 Double P46 and 3811 and Late flowering was and, and AT- and G1073 transcription factor P448 3936 enhanced compared to G1073 hook (63-71, 18 overexpression; either parental line 71-216) const. 35S prom. G481 CAAT (20-109 10 G481 and Double P46 and 3811 and Darker green leaves, and and AT- and G1073 transcription factor P448 3936 additive phenotype G1073 hook (63-71, 18 overexpression; compared to either 71-216) const. 35S prom. parental overexpressor line G481 CAAT (20-109) 10 G481 and Double P46 and 3811 and Darker green leaves, and and and G867 transcription factor P26372 4966 additive phenotype G867 AP2 (59-124, 16 overexpression; compared to either 184-276) const. 35S prom. parental overexpressor line G28 AP2 (145-208) 2 G28 and Double P174 and 3854 and More res. to Botrytis; and and and G1266 transcription factor P26385 4969 additive phenotype G1266 AP2 (79-147) 884 overexpression; relative to either const. 35S prom. parental overexpressor line G28 AP2 (145-208) 2 G28 and Double P174 and 3854 and Greater res. to and and and G1266 transcription factor P26385 4969 Fusarium; new G1266 AP2 (79-147) 884 overexpression; phenotype not const. 35S prom. previously observed in either parental overexpressor line G28 AP2 (145-208) 2 G28 and Double P174 and 3854 and Greater res. to and and and G1266 transcription factor P26385 4969 Sclerotinia; additive G1266 AP2 (79-147) 884 overexpression; phenotype relative to const. 35S prom. either parental overexpressor line G28 AP2 (145-208) 2 G28 and Double P174 and 3854 and Greater res. to and and and G1919 transcription factor P26383 4968 Fusarium; new G1919 RING/C3HC4 1268 overexpression; phenotype not (214-287) const. 35S prom. previously observed in either parental overexpressor line G1073 AT-hook 18 G1073 and Double P448 and 3936 and Reduced apical and (63-71, 71-216) and G1274 transcription factor P15038 4665 dominance G1274 and 20 overexpression; characteristic of G1274 WRKY const. 35S prom. OE lines, indicating (110-166) that G1274 OE can overcome increased branching effects of G1073 OE G47, AP2 (10-75), 6, G47, G481 Double and triple P26388, 4970, Water deficit G481 CAAT 10, and, G1073 transcription factor P46, and 3811, and (determined in a and, (20-109) and overexpression; P448 3936 drought assay*) tol. G1073 and AT- 18 const. 35S prom. was more marked than hook (63-71, was typically obtained 71-216) with any of the parental overexpressor lines G481, CAAT (20-109), 10, G481 Triple P46, 3811, Greater tol. to water G1073 AT- 18, G1073 and transcription factor P448, and 3936, and deficit (determined in a and hook (63-71, and G3086 overexpression, P15046 4668 drought assay*); G3086 71-216) 1836 const. 35S prom. flowered at the same and time as wild-type in HLH/MYC contrast to late (307-365) flowering in double G1073-G481 OEs; thus, G3086 OE mitigates delayed flowering or maturation associated with G481 and G1073 OE Abbreviations for Table 36: At: Arabidopsis thaliana; Bo: Brassica oleracea; Cs: Br: Brassica rapa; Citrus sinensis; Dc: Daucus carota; Gm: Glycine max; Os: Oryza sativa; Ga: Gossypium arboreum; Gh: Gossypium hirsutum; Gr: Gossypium raimondii; Mt: Medicago truncatula; Nb: Nicotiana benthamiana; Nt: Nicotiana tabacum; Pt: Populus trichocarpa; Sc: Saccharomyces cerevisiae; Sl: Solanum lycopersicum; So: Saccharum officinarum; St: Solanum tuberosum; Ta: Triticum aestivum; Vv: Vitis vinifera; Ze: Zinnia elegans; Zm: Zea mays ABA = abscisic acid; ACC = 1-aminocyclopropane 1-carboxylic acid; OE = overexpress(ed), overexpression or overexpressor(s); inc. = increase(d); tol. = tolerance; res. = resistance; sens. = sensitive; const. = constitutive; prom. = promoter; 35S = cauliflower mosaic virus 35S promoter; PEG = polyethylene glycol *drought tolerance determined in soil-based assays as opposed to plate-based drought or dehydration assays

In this Example, unless otherwise indicted, morphological and physiological traits are disclosed in comparison to wild-type control plants. That is, a transformed plant that is described as large and/or drought tolerant is large and more tolerant to drought with respect to a wild-type control plant. When a plant is said to have a better performance than controls, it generally showed less stress symptoms than control plants. The better performing lines may, for example, produce less anthocyanin, or be larger, green, or more vigorous in response to a particular stress, as noted below. Better performance generally implies greater tolerance to a particular biotic or abiotic stress, less sensitivity to ABA, or better recovery from a stress (as in the case of a drought treatment) than controls.

Example XIII Transformation of Eudicots for Greater Biomass, Disease Resistance or Abiotic Stress Tolerance

Crop species including tomato and soybean plants that overexpress any of a considerable number of the transcription factor polypeptides of the invention have been shown experimentally to produce plants with increased drought tolerance and/or biomass in field trials. For example, tomato plants overexpressing the G2153 polypeptide have been found to be larger than wild-type control tomato plants. For example, soy plants overexpressing a number of G481, G682, G867 and G1073, their orthologs or putative orthologs, and other sequences listed above have been shown to be more water deficit-tolerant than control plants. These observations indicate that these genes, when overexpressed, will result in larger yields than non-transformed plants in both stressed and non-stressed conditions.

Thus, transcription factor polynucleotide sequences listed in the Sequence Listing recombined into, for example, one of the expression vectors of the invention, or another suitable expression vector, may be transformed into a plant for the purpose of modifying plant traits for the purpose of improving yield and/or quality. The expression vector may contain a constitutive, tissue-specific or inducible promoter operably linked to the transcription factor polynucleotide. The cloning vector may be introduced into a variety of plants by means well known in the art such as, for example, direct DNA transfer or Agrobacterium tumefaciens-mediated transformation. It is now routine to produce transgenic plants using most eudicot plants (see Weissbach and Weissbach, (1989); Gelvin et al. (1990); Herrera-Estrella et al. (1983); Bevan (1984); and Klee (1985)). Methods for analysis of traits are routine in the art and examples are disclosed above.

Numerous protocols for the transformation of tomato and soy plants have been previously described, and are well known in the art. Gruber et al. (1993), and Glick and Thompson (1993) describe several expression vectors and culture methods that may be used for cell or tissue transformation and subsequent regeneration. For soybean transformation, methods are described by Miki et al. (1993); and U.S. Pat. No. 5,563,055, (Townsend and Thomas), issued Oct. 8, 1996.

There are a substantial number of alternatives to Agrobacterium-mediated transformation protocols, other methods for the purpose of transferring exogenous genes into soybeans or tomatoes. One such method is microprojectile-mediated transformation, in which DNA on the surface of microprojectile particles is driven into plant tissues with a biolistic device (see, for example, Sanford et al. (1987); Christou et al. (1992); Sanford (1993); Klein et al. (1987); U.S. Pat. No. 5,015,580 (Christou et al), issued May 14, 1991; and U.S. Pat. No. 5,322,783 (Tomes et al.), issued Jun. 21, 1994).

Alternatively, sonication methods (see, for example, Zhang et al. (1991)); direct uptake of DNA into protoplasts using CaCl₂ precipitation, polyvinyl alcohol or poly-L-ornithine (Hain et al. (1985); Draper et al. (1982)); liposome or spheroplast fusion (see, for example, Deshayes et al. (1985); Christou et al. (1987)); and electroporation of protoplasts and whole cells and tissues (see, for example, Donn et al.(1990); D'Halluin et al. (1992); and Spencer et al. (1994)) have been used to introduce foreign DNA and expression vectors into plants.

After a plant or plant cell is transformed (and the latter regenerated into a plant), the transformed plant may be crossed with itself or a plant from the same line, a non-transformed or wild-type plant, or another transformed plant from a different transgenic line of plants. Crossing provides the advantages of producing new and often stable transgenic varieties. Genes and the traits they confer that have been introduced into a tomato or soybean line may be moved into distinct line of plants using traditional backcrossing techniques well known in the art. Transformation of tomato plants may be conducted using the protocols of Koornneef et al (1986), and in U.S. Pat. No. 6,613,962, the latter method described in brief here. Eight day old cotyledon explants are precultured for 24 hours in Petri dishes containing a feeder layer of Petunia hybrida suspension cells plated on MS medium with 2% (w/v) sucrose and 0.8% agar supplemented with 10 μM α-naphthalene acetic acid and 4.4 μM 6-benzylaminopurine. The explants are then infected with a diluted overnight culture of Agrobacterium tumefaciens containing an expression vector comprising a polynucleotide of the invention for 5-10 minutes, blotted dry on sterile filter paper and cocultured for 48 hours on the original feeder layer plates. Culture conditions are as described above. Overnight cultures of Agrobacterium tumefaciens are diluted in liquid MS medium with 2% (w/v/) sucrose, pH 5.7) to an OD₆₀₀ of 0.8.

Following cocultivation, the cotyledon explants are transferred to Petri dishes with selective medium comprising MS medium with 4.56 μM zeatin, 67.3 μM vancomycin, 418.9 μM cefotaxime and 171.6 μM kanamycin sulfate, and cultured under the culture conditions described above. The explants are subcultured every three weeks onto fresh medium. Emerging shoots are dissected from the underlying callus and transferred to glass jars with selective medium without zeatin to form roots. The formation of roots in a kanamycin sulfate-containing medium is a positive indication of a successful transformation.

Transformation of soybean plants may be conducted using the methods found in, for example, U.S. Pat. No. 5,563,055 (Townsend et al., issued Oct. 8, 1996), described in brief here. In this method soybean seed is surface sterilized by exposure to chlorine gas evolved in a glass bell jar. Seeds are germinated by plating on 1/10 strength agar solidified medium without plant growth regulators and culturing at 28° C. with a 16 hour day length. After three or four days, seed may be prepared for cocultivation. The seedcoat is removed and the elongating radical removed 3-4 mm below the cotyledons.

Overnight cultures of Agrobacterium tumefaciens harboring the expression vector comprising a polynucleotide of the invention are grown to log phase, pooled, and concentrated by centrifugation. Inoculations are conducted in batches such that each plate of seed is treated with a newly resuspended pellet of Agrobacterium. The pellets are resuspended in 20 ml inoculation medium. The inoculum is poured into a Petri dish containing prepared seed and the cotyledonary nodes are macerated with a surgical blade. After 30 minutes the explants are transferred to plates of the same medium that has been solidified. Explants are embedded with the adaxial side up and level with the surface of the medium and cultured at 22° C. for three days under white fluorescent light. These plants may then be regenerated according to methods well established in the art, such as by moving the explants after three days to a liquid counter-selection medium (see U.S. Pat. No. 5,563,055).

The explants may then be picked, embedded and cultured in solidified selection medium. After one month on selective media transformed tissue becomes visible as green sectors of regenerating tissue against a background of bleached, less healthy tissue. Explants with green sectors are transferred to an elongation medium. Culture is continued on this medium with transfers to fresh plates every two weeks. When shoots are 0.5 cm in length they may be excised at the base and placed in a rooting medium.

Example XIV Transformation of Monocots for Greater Biomass, Disease Resistance or Abiotic Stress Tolerance

Cereal plants such as, but not limited to, corn, wheat, rice, sorghum, barley, switchgrass or Miscanthus may be transformed with the present polynucleotide sequences, including monocot or eudicot-derived sequences such as those presented in the present Tables, cloned into a vector such as pGA643 and containing a kanamycin-resistance marker, and expressed constitutively under, for example, the CaMV 35S or COR15 promoters, or with tissue-specific or inducible promoters. The expression vectors may be one found in the Sequence Listing, or any other suitable expression vector may be similarly used. For example, pMEN020 may be modified to replace the NptII coding region with the BAR gene of Streptomyces hygroscopicus that confers resistance to phosphinothricin. The KpnI and BglII sites of the Bar gene are removed by site-directed mutagenesis with silent codon changes.

The cloning vector may be introduced into a variety of cereal plants by means well known in the art including direct DNA transfer or Agrobacterium tumefaciens-mediated transformation. The latter approach may be accomplished by a variety of means, including, for example, that of U.S. Pat. No. 5,591,616, in which monocotyledon callus is transformed by contacting dedifferentiating tissue with the Agrobacterium containing the cloning vector.

The sample tissues are immersed in a suspension of 3×10⁹ cells of Agrobacterium containing the cloning vector for 3-10 minutes. The callus material is cultured on solid medium at 25° C. in the dark for several days. The calli grown on this medium are transferred to Regeneration medium. Transfers are continued every 2-3 weeks (2 or 3 times) until shoots develop. Shoots are then transferred to Shoot-Elongation medium every 2-3 weeks. Healthy looking shoots are transferred to rooting medium and after roots have developed, the plants are placed into moist potting soil.

The transformed plants are then analyzed for the presence of the NPTII gene/kanamycin resistance by ELISA, using the ELISA NPTII kit from SPrime-3Prime Inc. (Boulder, Colo.).

It is also routine to use other methods to produce transgenic plants of most cereal crops (Vasil (1994)) such as corn, wheat, rice, sorghum (Cassas et al. (1993)), and barley (Wan and Lemeaux (1994)). DNA transfer methods such as the microprojectile method can be used for corn (Fromm et al. (1990); Gordon-Kamm et al. (1990); Ishida (1990)), wheat (Vasil et al. (1992); Vasil et al. (1993); Weeks et al. (1993)), and rice (Christou (1991); Hiei et al. (1994); Aldemita and Hodges (1996); and Hiei et al. (1997)). For most cereal plants, embryogenic cells derived from immature scutellum tissues are the preferred cellular targets for transformation (Hiei et al. (1997); Vasil (1994)). For transforming corn embryogenic cells derived from immature scutellar tissue using microprojectile bombardment, the A188XB73 genotype is the preferred genotype (Fromm et al. (1990); Gordon-Kamm et al. (1990)). After microprojectile bombardment the tissues are selected on phosphinothricin to identify the transgenic embryogenic cells (Gordon-Kamm et al. (1990)). Transgenic plants are regenerated by standard corn regeneration techniques (Fromm et al. (1990); Gordon-Kamm et al. (1990)).

Example XV Transcription Factor Expression and Analysis of Disease Resistance or Abiotic Stress Tolerance

Northern blot analysis, RT-PCR or microarray analysis of the regenerated, transformed plants may be used to show expression of a transcription factor polypeptide or the invention and related genes that are capable of inducing disease resistance, abiotic stress tolerance, and/or larger size.

To verify the ability to confer stress resistance, mature plants overexpressing a transcription factor of the invention, or alternatively, seedling progeny of these plants, may be challenged by a stress such as a disease pathogen, drought, heat, cold, high salt, or desiccation. Alternatively, these plants may challenged in a hyperosmotic stress condition that may also measure altered sugar sensing, such as a high sugar condition. By comparing control plants (for example, wild type) and transgenic plants similarly treated, the transgenic plants may be shown to have greater tolerance to the particular stress.

After a eudicot plant, monocot plant or plant cell has been transformed (and the latter regenerated into a plant) and shown to have greater size or tolerance to abiotic stress, or produce greater yield relative to a control plant under the stress conditions, the transformed monocot plant may be crossed with itself or a plant from the same line, a non-transformed or wild-type monocot plant, or another transformed monocot plant from a different transgenic line of plants.

These experiments would demonstrate that transcription factor polypeptides of the invention can be identified and shown to confer larger size, greater yield, greater disease resistance and/or abiotic stress tolerance in eudicots or monocots, including tolerance or resistance to multiple stresses.

Example XVI Sequences that Confer Significant Improvements to Non-Arabidopsis Species

The function of specific transcription factors of the invention, including closely-related orthologs, have been analyzed and may be further characterized and incorporated into crop plants. The ectopic overexpression of these sequences may be regulated using constitutive, inducible, or tissue specific regulatory elements. Genes that have been examined and have been shown to modify plant traits (including increasing biomass, disease resistance and/or abiotic stress tolerance) encode transcription factor polypeptides found in the Sequence Listing. In addition to these sequences, it is expected that newly discovered polynucleotide and polypeptide sequences closely related to polynucleotide and polypeptide sequences found in the Sequence Listing can also confer alteration of traits in a similar manner to the sequences found in the Sequence Listing, when transformed into a any of a considerable variety of plants of different species, and including eudicots and monocots. The polynucleotide and polypeptide sequences derived from monocots (e.g., the rice sequences) may be used to transform both monocot and eudicot plants, and those derived from eudicots (e.g., the Arabidopsis and soy genes) may be used to transform either group, although it is expected that some of these sequences will function best if the gene is transformed into a plant from the same group as that from which the sequence is derived.

As an example of a first step to determine drought-related tolerance, seeds of these transgenic plants are subjected to germination assays to measure sucrose sensing. Sterile monocot seeds, including, but not limited to, corn, rice, wheat, rye and sorghum, as well as eudicots including, but not limited to soybean and alfalfa, are sown on 80% MS medium plus vitamins with 9.4% sucrose; control media lack sucrose. All assay plates are then incubated at 22° C. under 24-hour light, 120-130 μEin/m²/s, in a growth chamber. Evaluation of germination and seedling vigor is then conducted three days after planting. Plants overexpressing sequences of the invention may be found to be more tolerant to high sucrose by having better germination, longer radicals, and more cotyledon expansion. These methods have been used to show that overexpressors of numerous sequences of the invention are involved in sucrose-specific sugar sensing. It is expected that structurally similar orthologs of these sequences, including those found in the Sequence Listing, are also involved in sugar sensing, an indication of altered osmotic stress tolerance.

Plants overexpressing the transcription factor sequences of the invention may also be subjected to soil-based drought assays to identify those lines that are more tolerant to water deprivation than wild-type control plants. A number of the lines of plants overexpressing transcription factor polypeptides of the invention, including newly discovered closely-related species, will be significantly larger and greener, with less wilting or desiccation, than wild-type control plants, particularly after a period of water deprivation is followed by rewatering and a subsequent incubation period. The sequence of the transcription factor may be overexpressed under the regulatory control of constitutive, tissue specific or inducible promoters, or may comprise a GAL4 transactivation domain fused to either the N— or the C terminus of the polypeptide. The results presented in Examples above indicate that these transcription factors may confer disease resistance or abiotic stress tolerance when they are overexpressed under the regulatory control of non-constitutive promoters or a transactivation domain fused to the clade member, without having a significant adverse impact on plant morphology and/or development. The lines that display useful traits may be selected for further study or commercial development.

Monocotyledonous plants, including rice, corn, wheat, rye, sorghum, barley and others, may be transformed with a plasmid containing a transcription factor polynucleotide. The transcription factor gene sequence may include eudicot or monocot-derived sequences such as those presented herein. These transcription factor genes may be cloned into an expression vector containing a kanamycin-resistance marker, and then expressed constitutively or in a tissue-specific or inducible manner.

The cloning vector may be introduced into monocots by, for example, means described in the previous Example, including direct DNA transfer or Agrobacterium tumefaciens-mediated transformation. The latter approach may be accomplished by a variety of means, including, for example, that of U.S. Pat. No. 5,591,616, in which monocotyledon callus is transformed by contacting dedifferentiating tissue with the Agrobacterium containing the cloning vector.

The sample tissues are immersed in a suspension of 3×10⁻⁹ cells of Agrobacterium containing the cloning vector for 3-10 minutes. The callus material is cultured on solid medium at 25° C. in the dark for several days. The calli grown on this medium are transferred to Regeneration medium. Transfers are continued every 2-3 weeks (2 or 3 times) until shoots develop. Shoots are then transferred to Shoot-Elongation medium every 2-3 weeks. Healthy looking shoots are transferred to rooting medium and after roots have developed, the plants are placed into moist potting soil.

The transformed plants are then analyzed for the presence of the NPTII gene/kanamycin resistance by ELISA, using the ELISA NPTII kit from SPrime-3Prime Inc. (Boulder, Colo.).

Northern blot analysis, RT-PCR or microarray analysis of the regenerated, transformed plants may be used to show expression of a transcription factor polypeptide of the invention that is capable of conferring abiotic stress tolerance, disease resistance, or increased size or yield, in the transformed plants.

To verify the ability to confer abiotic stress tolerance, mature plants or seedling progeny of these plants expressing a monocot-derived equivalog gene may be challenged using methods described in the above Examples. By comparing wild type plants and the transgenic plants, the latter are shown be more tolerant to abiotic stress, more resistant to disease, and/or have greater biomass, as compared to wild type control plants similarly treated.

It is expected that the same methods may be applied to identify other useful and valuable sequences of the present transcription factor clades, and the sequences may be derived from a diverse range of species.

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All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

The present invention is not limited by the specific embodiments described herein. The invention now being fully described, it will be apparent to one of ordinary skill in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the appended claims. Modifications that become apparent from the foregoing description and accompanying figures fall within the scope of the claims. 

1. An nucleic acid construct comprising a recombinant nucleic acid sequence encoding a polypeptide, wherein: the polypeptide shares an amino acid identity with any of SEQ ID NO: 2n, where n=1 to 1186, or SEQ ID NO: 2373-3791, wherein the percent amino acid identity is selected from the group consisting of at least about 54%, at least about 55%, at least about 56%, at least about 57%, at least about 58%, at least about 59%, at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, and about 100%; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps at least as stringent as 6×SSC at 65° C. of 10-30 minutes for each wash step; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps of 0.2× to 2×SSC and 0.1% SDS at 50° C. to 65° C. for 10-30 minutes per wash step; wherein when the polypeptide is overexpressed in a plant, the polypeptide regulates transcription and confers at least one regulatory activity resulting in an altered trait in the plant as compared to a control plant, and the altered trait is selected from the group consisting of: greater resistance to Erysiphe; greater resistance to Sclerotinia; greater resistance to Botrytis; greater resistance to Fusarium; greater susceptibility to Sclerotinia; greater susceptibility to Botrytis; greater tolerance to Pseudomonas; greater tolerance to dehydration; greater tolerance to drought; greater tolerance to salt; greater tolerance to water deficit conditions; greater tolerance to hyperosmotic stress; greater tolerance to low nitrogen conditions; greater tolerance to low phosphate conditions; greater tolerance to low potassium conditions; greater tolerance to cold; greater tolerance to heat; greater tolerance to sucrose; greater tolerance to mannitol; greater tolerance to glucose; greater tolerance to polyethylene glycol; greater tolerance to glyphosate; greater tolerance to oxidative stress; greater tolerance to freezing; better recovery from drought; more sensitive to cold; more sensitive to low nitrogen conditions; more sensitive to low phosphate conditions; more sensitive to sucrose; more sensitive to mannitol; more sensitive to glucose; more sensitive to drought; more sensitive to heat; more sensitive to hyperosmotic stress; more sensitive to oxidative stress; more sensitive to ethylene; ethylene insensitive when germinated in the dark on 1-aminocyclopropane 1-carboxylic acid; hypersensitive to 1-aminocyclopropane 1-carboxylic acid; decreased sensitivity to ABA; altered C/N sensing; higher starch level; higher proline level; decreased proline level; darker green color; lighter green color; gray color; greater photosynthetic capacity; reduced photosynthesis; increased chlorophyll level; more chlorophyll a and b; higher total nitrogen concentration level; decreased chlorophyll level; more pigment; greater anthocyanin level; greater leaf anthocyanin level; more anthocyanin in leaf petioles; decreased anthocyanin level; greater carotenoid level; greater ABA level; greater seed oil content; greater seed protein content; greater seed oil content; greater seed protein content; greater total seed oil and protein content; increased seed alpha-tocopherol level; higher seed lutein content; decreased seed lutein content; increased seed xanthophyll I level; increase in seed 16:1 fatty acids level; increased seed 18:1 fatty acids level; increased seed 18:2 fatty acids and decrease in seed 18:3 fatty acids level; increased seed 18:1 and 18:2 fatty acids level; increased seed 16:0, 18:0, 20:0, and 18:3 fatty acids, decreased seed 18:2, 20:1, 22:1 fatty acids level; decreased seed 20:1 and 22:1 fatty acids level; decrease in seed 18:1 seed fatty acids level; decrease in 18:2 fatty acids level; altered seed glucosinolate profile; up-regulation of genes involved in secondary metabolism; altered leaf prenyl lipids; reduced chlorophyll a and b levels; increased leaf insoluble sugars level; decreased leaf insoluble sugars level; increased galactose level in leaf cell wall; increased leaf xanthophyll; increased leaf rhamnose level; increased leaf mannose; increased leaf fucose level; increased leaf glucosinolate M39480 level; increased leaf glucosinolate M39481 level; decreased leaf rhamnose level; decreased leaf lutein level; more leaf fatty acids; altered leaf fatty acid composition; reduced leaf 16:3 fatty acids; increased in percentage of 16:0 leaf fatty acids; leaf 16:0 level decreased and leaf 16:3 level increased; greater seedling vigor; faster seedling growth; slower growth; late flowering; late developing; early flowering; early developing; glossy leaves; waxy leaves; more lignin; reduced lignin; reduced internode elongation; short internodes; long internodes; defect in cell elongation; greater internode distance; altered cotyledon shape; elongated cotyledons; cotyledon fusion; thicker stem; altered distribution of stem vascular bundles; reduced branching; curled leaves; serrated leaves; curled leaves; ovoid leaves; flat leaves; heart-shaped leaves; longer leaves; narrower leaves; wrinkled leaves; lobed leaves; light green leaves; larger, flatter leaves at late stage of development; greater number of leaves; altered flowers; abnormal flowers; sporadic defects in flower development; reduced fertility; flowers that do not open; floral organs with bract-like features; bolts that terminate without an inflorescence; aerial rosettes; reduced floral organ abscission; delayed floral organ abscission; reductions in flower organ size; larger floral organs; long flower organs; long sepal and petal; poor anther dehiscence; little pollen production; no pollen production; poor filament elongation; homeotic transformations; bushy inflorescences; altered inflorescences; flowers bunched together; short inflorescence stems; stunted inflorescence growth; numerous secondary inflorescence meristems; altered inflorescence determinacy; homeotic transformation; terminal flower formation; increased carpel size; wider carpels; ectopic carpel tissue; filamentous carpelloid growths on flower pedicels; loss of flower determinacy; floral organ abscission delayed; altered seed color; pale seeds; smaller seeds; rounded seeds; wrinkled seeds; wrinkled sickle-shaped siliques; reduced flower petal number; reduced flower sepal number; reduced flower stamen number; smaller petals and sepals; delayed senescence; premature senescence; premature leaf senescence; premature flower senescence; trilocular silique; more root mass; reduced secondary root growth; greater leaf and hypocotyl necrosis; short pedicels; short inflorescence stems; altered leaf cell expansion; reduced cell differentiation in meristem; increased necrosis; lethal when constitutively overexpressed; embryo lethal; altered light response; long cotyledons; open cotyledons; oval cotyledons; long hypocotyls; long petioles; leaves in a more upright orientation; constitutive photomorphogenesis; more root growth in the dark; greater biomass; larger plants; large darker green rosettes at late stage of development; larger seeds; larger leaves; smaller plants; more root hairs; fewer trichomes; greater trichome size and density; greater trichome density; ectopic trichome formation; ectopic formation of trichomes on abaxial leaf surfaces; greater trichome density on sepals and ectopic trichomes on carpels.
 2. The nucleic acid construct of claim 1, wherein the stringent conditions comprising two wash steps of 0.5×SSC, 0.1% SDS at 65° C. of 10-30 minutes for each wash step.
 3. The nucleic acid construct of claim 1, wherein expression of the polypeptide is regulated by a constitutive, inducible, or tissue-specific promoter.
 4. A recombinant host cell comprising a nucleic acid construct of claim
 1. 5. A transgenic plant having an altered trait as compared to a control plant, wherein the transgenic plant comprises: at least one nucleic acid construct comprising a recombinant nucleic acid sequence encoding a polypeptide, wherein: the polypeptide shares an amino acid identity with any of SEQ ID NO: 2n, where n=1 to 1186, or SEQ ID NO: 2373-3791, wherein the percent amino acid identity is selected from the group consisting of at least about 54%, at least about 55%, at least about 56%, at least about 57%, at least about 58%, at least about 59%, at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%,at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, and about 100%; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps at least as stringent as 6×SSC at 65° C. of 10-30 minutes for each wash step; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps of 0.2× to 2×SSC and 0.1% SDS at 50° C. to 65° C. for 10-30 minutes per wash step; wherein when the polypeptide is overexpressed in a plant, the polypeptide regulates transcription and confers at least one regulatory activity resulting in the altered trait in the plant as compared to a control plant; and the altered trait is selected from the group consisting of: greater resistance to Erysiphe; greater resistance to Sclerotinia; greater resistance to Botrytis; greater resistance to Fusarium; greater susceptibility to Sclerotinia; greater susceptibility to Botrytis; greater tolerance to Pseudomonas; greater tolerance to dehydration; greater tolerance to drought; greater tolerance to salt; greater tolerance to water deficit conditions; greater tolerance to hyperosmotic stress; greater tolerance to low nitrogen conditions; greater tolerance to low phosphate conditions; greater tolerance to low potassium conditions; greater tolerance to cold; greater tolerance to heat; greater tolerance to sucrose; greater tolerance to mannitol; greater tolerance to glucose; greater tolerance to polyethylene glycol; greater tolerance to glyphosate; greater tolerance to oxidative stress; greater tolerance to freezing; better recovery from drought; more sensitive to cold; more sensitive to low nitrogen conditions; more sensitive to low phosphate conditions; more sensitive to sucrose; more sensitive to mannitol; more sensitive to glucose; more sensitive to drought; more sensitive to heat; more sensitive to hyperosmotic stress; more sensitive to oxidative stress; more sensitive to ethylene; ethylene insensitive when germinated in the dark on 1-aminocyclopropane 1-carboxylic acid; hypersensitive to 1-aminocyclopropane 1-carboxylic acid; decreased sensitivity to ABA; altered C/N sensing; higher starch level; higher proline level; decreased proline level; darker green color; lighter green color; gray color; greater photosynthetic capacity; reduced photosynthesis; increased chlorophyll level; more chlorophyll a and b; higher total nitrogen concentration level; decreased chlorophyll level; more pigment; greater anthocyanin level; greater leaf anthocyanin level; more anthocyanin in leaf petioles; decreased anthocyanin level; greater carotenoid level; greater ABA level; greater seed oil content; greater seed protein content; greater seed oil content; greater seed protein content; greater total seed oil and protein content; increased seed alpha-tocopherol level; higher seed lutein content; decreased seed lutein content; increased seed xanthophyll I level; increase in seed 16:1 fatty acids level; increased seed 18:1 fatty acids level; increased seed 18:2 fatty acids and decrease in seed 18:3 fatty acids level; increased seed 18:1 and 18:2 fatty acids level; increased seed 16:0, 18:0, 20:0, and 18:3 fatty acids, decreased seed 18:2, 20:1, 22:1 fatty acids level; decreased seed 20:1 and 22:1 fatty acids level; decrease in seed 18:1 seed fatty acids level; decrease in 18:2 fatty acids level; altered seed glucosinolate profile; up-regulation of genes involved in secondary metabolism; altered leaf prenyl lipids; reduced chlorophyll a and b levels; increased leaf insoluble sugars level; decreased leaf insoluble sugars level; increased galactose level in leaf cell wall; increased leaf xanthophyll; increased leaf rhamnose level; increased leaf mannose; increased leaf fucose level; increased leaf glucosinolate M39480 level; increased leaf glucosinolate M39481 level; decreased leaf rhamnose level; decreased leaf lutein level; more leaf fatty acids; altered leaf fatty acid composition; reduced leaf 16:3 fatty acids; increased in percentage of 16:0 leaf fatty acids; leaf 16:0 level decreased and leaf 16:3 level increased; greater seedling vigor; faster seedling growth; slower growth; late flowering; late developing; early flowering; early developing; glossy leaves; waxy leaves; more lignin; reduced lignin; reduced internode elongation; short internodes; long internodes; defect in cell elongation; greater internode distance; altered cotyledon shape; elongated cotyledons; cotyledon fusion; thicker stem; altered distribution of stem vascular bundles; reduced branching; curled leaves; serrated leaves; curled leaves; ovoid leaves; flat leaves; heart-shaped leaves; longer leaves; narrower leaves; wrinkled leaves; lobed leaves; light green leaves; larger, flatter leaves at late stage of development; greater number of leaves; altered flowers; abnormal flowers; sporadic defects in flower development; reduced fertility; flowers that do not open; floral organs with bract-like features; bolts that terminate without an inflorescence; aerial rosettes; reduced floral organ abscission; delayed floral organ abscission; reductions in flower organ size; larger floral organs; long flower organs; long sepal and petal; poor anther dehiscence; little pollen production; no pollen production; poor filament elongation; homeotic transformations; bushy inflorescences; altered inflorescences; flowers bunched together; short inflorescence stems; stunted inflorescence growth; numerous secondary inflorescence meristems; altered inflorescence determinacy; homeotic transformation; terminal flower formation; increased carpel size; wider carpels; ectopic carpel tissue; filamentous carpelloid growths on flower pedicels; loss of flower determinacy; floral organ abscission delayed; altered seed color; pale seeds; smaller seeds; rounded seeds; wrinkled seeds; wrinkled sickle-shaped siliques; reduced flower petal number; reduced flower sepal number; reduced flower stamen number; smaller petals and sepals; delayed senescence; premature senescence; premature leaf senescence; premature flower senescence; trilocular silique; more root mass; reduced secondary root growth; greater leaf and hypocotyl necrosis; short pedicels; short inflorescence stems; altered leaf cell expansion; reduced cell differentiation in meristem; increased necrosis; lethal when constitutively overexpressed; embryo lethal; altered light response; long cotyledons; open cotyledons; oval cotyledons; long hypocotyls; long petioles; leaves in a more upright orientation; constitutive photomorphogenesis; more root growth in the dark; greater biomass; larger plants; large darker green rosettes at late stage of development; larger seeds; larger leaves; smaller plants; more root hairs; fewer trichomes; greater trichome size and density; greater trichome density; ectopic trichome formation; ectopic formation of trichomes on abaxial leaf surfaces; greater trichome density on sepals and ectopic trichomes on carpels.
 6. The transgenic plant of claim 5, wherein the transgenic plant is a eudicot.
 7. The transgenic plant of claim 5, wherein the transgenic plant is a legume.
 8. The transgenic plant of claim 5, wherein the transgenic plant is a monocot.
 9. The transgenic plant of claim 5, wherein the transgenic plant is a transgenic seed comprising the recombinant nucleic acid sequence.
 10. The transgenic plant of claim 5, wherein the transgenic plant overexpresses two or more transcription factor polypeptides, wherein: (a) the two or more transcription factor polypeptides include the pair of SEQ ID NOs: 18 and 20, or the pair of SEQ ID NOs: 18 and 1836, or the pair of SEQ ID NOs: 10 and 20, or the pair of SEQ ID NOs: 10 and 18, or the pair of SEQ ID NOs: 10 and 16, or the pair of SEQ ID NOs: 2 and 884, or the pair of SEQ ID NOs: 2 and 1268, or the pair of SEQ ID NOs: 18 and 20, or each of SEQ ID NOs: 6, 10, and 18, or each of SEQ ID NOs: 10, 18, and
 1836. 11. A method for conferring to a plant an altered trait as compared to a control plant, the method comprising: (a) providing at least one nucleic acid construct comprising a recombinant nucleic acid sequence encoding a polypeptide, wherein: the polypeptide shares an amino acid identity with any of SEQ ID NO: 2n, where n=1 to 1186, or SEQ ID NO: 2373-3791, wherein the percent amino acid identity is selected from the group consisting of at least about 54%, at least about 55%, at least about 56%, at least about 57%, at least about 58%, at least about 59%, at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, and about 100%; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps at least as stringent as 6×SSC at 65° C. of 10-30 minutes for each wash step; or the recombinant nucleic acid sequence specifically hybridizes to the complement of the sequence set forth in SEQ ID NO: 2n−1, where n=1 to 1186, or SEQ ID NO: 3792 to 5106 under stringent conditions comprising two wash steps of 0.2× to 2×SSC and 0.1% SDS at 50° C. to 65° C. for 10-30 minutes per wash step; wherein when the polypeptide is overexpressed in a plant, the polypeptide regulates transcription and confers at least one regulatory activity resulting in the altered trait in the plant as compared to a control plant; and (b) transforming a target plant with the at least one nucleic acid construct to produce a transgenic plant having the altered trait as compared to the control plant; wherein the altered trait is selected from the group consisting of: greater resistance to Erysiphe; greater resistance to Sclerotinia; greater resistance to Botrytis; greater resistance to Fusarium; greater susceptibility to Sclerotinia; greater susceptibility to Botrytis; greater tolerance to Pseudomonas; greater tolerance to dehydration; greater tolerance to drought; greater tolerance to salt; greater tolerance to water deficit conditions; greater tolerance to hyperosmotic stress; greater tolerance to low nitrogen conditions; greater tolerance to low phosphate conditions; greater tolerance to low potassium conditions; greater tolerance to cold; greater tolerance to heat; greater tolerance to sucrose; greater tolerance to mannitol; greater tolerance to glucose; greater tolerance to polyethylene glycol; greater tolerance to glyphosate; greater tolerance to oxidative stress; greater tolerance to freezing; better recovery from drought; more sensitive to cold; more sensitive to low nitrogen conditions; more sensitive to low phosphate conditions; more sensitive to sucrose; more sensitive to mannitol; more sensitive to glucose; more sensitive to drought; more sensitive to heat; more sensitive to hyperosmotic stress; more sensitive to oxidative stress; more sensitive to ethylene; ethylene insensitive when germinated in the dark on 1-aminocyclopropane 1-carboxylic acid; hypersensitive to 1-aminocyclopropane 1-carboxylic acid; decreased sensitivity to ABA; altered C/N sensing; higher starch level; higher proline level; decreased proline level; darker green color; lighter green color; gray color; greater photosynthetic capacity; reduced photosynthesis; increased chlorophyll level; more chlorophyll a and b; higher total nitrogen concentration level; decreased chlorophyll level; more pigment; greater anthocyanin level; greater leaf anthocyanin level; more anthocyanin in leaf petioles; decreased anthocyanin level; greater carotenoid level; greater ABA level; greater seed oil content; greater seed protein content; greater seed oil content; greater seed protein content; greater total seed oil and protein content; increased seed alpha-tocopherol level; higher seed lutein content; decreased seed lutein content; increased seed xanthophyll I level; increase in seed 16:1 fatty acids level; increased seed 18:1 fatty acids level; increased seed 18:2 fatty acids and decrease in seed 18:3 fatty acids level; increased seed 18:1 and 18:2 fatty acids level; increased seed 16:0, 18:0, 20:0, and 18:3 fatty acids, decreased seed 18:2, 20:1, 22:1 fatty acids level; decreased seed 20:1 and 22:1 fatty acids level; decrease in seed 18:1 seed fatty acids level; decrease in 18:2 fatty acids level; altered seed glucosinolate profile; up-regulation of genes involved in secondary metabolism; altered leaf prenyl lipids; reduced chlorophyll a and b levels; increased leaf insoluble sugars level; decreased leaf insoluble sugars level; increased galactose level in leaf cell wall; increased leaf xanthophyll; increased leaf rhamnose level; increased leaf mannose; increased leaf fucose level; increased leaf glucosinolate M39480 level; increased leaf glucosinolate M39481 level; decreased leaf rhamnose level; decreased leaf lutein level; more leaf fatty acids; altered leaf fatty acid composition; reduced leaf 16:3 fatty acids; increased in percentage of 16:0 leaf fatty acids; leaf 16:0 level decreased and leaf 16:3 level increased; greater seedling vigor; faster seedling growth; slower growth; late flowering; late developing; early flowering; early developing; glossy leaves; waxy leaves; more lignin; reduced lignin; reduced internode elongation; short internodes; long internodes; defect in cell elongation; greater internode distance; altered cotyledon shape; elongated cotyledons; cotyledon fusion; thicker stem; altered distribution of stem vascular bundles; reduced branching; curled leaves; serrated leaves; curled leaves; ovoid leaves; flat leaves; heart-shaped leaves; longer leaves; narrower leaves; wrinkled leaves; lobed leaves; light green leaves; larger, flatter leaves at late stage of development; greater number of leaves; altered flowers; abnormal flowers; sporadic defects in flower development; reduced fertility; flowers that do not open; floral organs with bract-like features; bolts that terminate without an inflorescence; aerial rosettes; reduced floral organ abscission; delayed floral organ abscission; reductions in flower organ size; larger floral organs; long flower organs; long sepal and petal; poor anther dehiscence; little pollen production; no pollen production; poor filament elongation; homeotic transformations; bushy inflorescences; altered inflorescences; flowers bunched together; short inflorescence stems; stunted inflorescence growth; numerous secondary inflorescence meristems; altered inflorescence determinacy; homeotic transformation; terminal flower formation; increased carpel size; wider carpels; ectopic carpel tissue; filamentous carpelloid growths on flower pedicels; loss of flower determinacy; floral organ abscission delayed; altered seed color; pale seeds; smaller seeds; rounded seeds; wrinkled seeds; wrinkled sickle-shaped siliques; reduced flower petal number; reduced flower sepal number; reduced flower stamen number; smaller petals and sepals; delayed senescence; premature senescence; premature leaf senescence; premature flower senescence; trilocular silique; more root mass; reduced secondary root growth; greater leaf and hypocotyl necrosis; short pedicels; short inflorescence stems; altered leaf cell expansion; reduced cell differentiation in meristem; increased necrosis; lethal when constitutively overexpressed; embryo lethal; altered light response; long cotyledons; open cotyledons; oval cotyledons; long hypocotyls; long petioles; leaves in a more upright orientation; constitutive photomorphogenesis; more root growth in the dark; greater biomass; larger plants; large darker green rosettes at late stage of development; larger seeds; larger leaves; smaller plants; more root hairs; fewer trichomes; greater trichome size and density; greater trichome density; ectopic trichome formation; ectopic formation of trichomes on abaxial leaf surfaces; greater trichome density on sepals and ectopic trichomes on carpels.
 12. The method of claim 11, wherein the methods further comprises the step of: (c) selecting a transgenic plant that ectopically expresses the polypeptide, and/or has the altered trait relative to the control plant.
 13. The method of claim 11, wherein the stringent conditions comprising two wash steps of 0.5×SSC, 0.1% SDS at 65° C. of 10-30 minutes for each wash step.
 14. The method of claim 11, wherein the method steps further comprises the step of: (c) selfing or crossing the transgenic plant with itself or another plant, respectively, to produce a transgenic seed. 